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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7003R-2

Symbol CG7003 Full Name CG7003

CG No CG7003 Old CG No CG7003

Synonyms Msh6, CG7003

Accession No (Link to NCBI) NM_140498.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGATGCATCCAAGAGCGAAA

Primer Seq. 3'

aatctagaggtaccATCATCGTAGGAAGCATCCTG

Predicted Fragment Size

IR fragment full Seq

0001 CCGNTGCATC CAAGAGCGAA AAGGAGAATC TCCAGAACCA GCAGCCAAAA GTGAAGGATG 
0061 GTAAGAAAGA GGCTTCCAAG CCGGCGGCCA AGAGGAAGTT GCCCATTTCC GACGATGAGC 
0121 CGATCAGTGG TCAACGCAAG CGGAAGCGCA TCGTTCAGCC GGAATCGGAC AGTGAGCCGG 
0181 AAATGGAGGT AACCAAGTCG GAGGACGACT TCTCCGATTG CGCCTCCGAC TACTGAACCG 
0241 GATGAGAATG AAGCCAGCGA TGATAGCGTT AGNAGCGGCG CGGNAAGGAG GTNGTCGCCT 
0301 AGCGAGAACG ACATGTCCGT GGACAGTCCG ACACCCAAGA AATCGCGCAA AAAGTCTAAG 
0361 ATTCTGAATA ACAACAATAA CAATGGAGCC ATCGAGCAAG AAGGTCAAGT CTAGAGTCGA 
0421 CCATCCAGTT GGCCGAGGGN NTGCAACTCT TTCAAGAAAA GCTGAAGAAC CTGCAGAGCA 
0481 ATGCCAAGCA GGATGCTTCC TACGATGAT

in silico PCR Fragment

0001 CCGATGCATC CAAGAGCGAA AAGGAGAATC TCCAGAACCA GCAGCCAAAA GTGAAGGATG 
0061 GTAAGAAAGA GGCTTCCAAG CCGGCGGCCA AGAGGAAGTT GCCCATTTCC GACGATGAGC 
0121 CGGCCAGTGG TCAACGCAAG CGGAAGCGCA TCGTTCAGCC GGAATCGGAC AGTGAGCCGG 
0181 AAATGGAGGT AACCAAGTCG GAGGACGACT TCTCCGATTG CGCCTCCGAC TAC-GAACCG 
0241 GATGAGAATG AAGCCAGCGA TGATAGCGTT AGCAGCGGCG CGG--AGGAG GT-GTCGCCT 
0301 AGCGAGAACG ACATGTCCGT GGACAGTCCG ACACCCAAGA AATCGCGCAA AAAGTCTAAG 
0361 ATTCTGAATA ACAACAATAA CAAT-GAGCC ATCGAGCAAG AAGGTCAAG- CTAGAGTCGA 
0421 CCATCCAGTT GGCCGAGGG- -TGCAAC-CT TTCAAGAAAA GCTGAAGAAC CTGCAGAGCA 
0481 ATGCCAAGCA GGATGCTTCC TACGATGAT

Assemble Data


7003R-2.IR_full       1   CCGNTGCATCCAAGAGCGAAAAGGAGAATCTCCAGAACCAGCAGCCAAAAGTGAAGGATG 60
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7003R-2.in silico     1   CCGATGCATCCAAGAGCGAAAAGGAGAATCTCCAGAACCAGCAGCCAAAAGTGAAGGATG 60


7003R-2.IR_full       61  GTAAGAAAGAGGCTTCCAAGCCGGCGGCCAAGAGGAAGTTGCCCATTTCCGACGATGAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7003R-2.in silico     61  GTAAGAAAGAGGCTTCCAAGCCGGCGGCCAAGAGGAAGTTGCCCATTTCCGACGATGAGC 120


7003R-2.IR_full       121 CGATCAGTGGTCAACGCAAGCGGAAGCGCATCGTTCAGCCGGAATCGGACAGTGAGCCGG 180
                          ||  ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7003R-2.in silico     121 CGGCCAGTGGTCAACGCAAGCGGAAGCGCATCGTTCAGCCGGAATCGGACAGTGAGCCGG 180


7003R-2.IR_full       181 AAATGGAGGTAACCAAGTCGGAGGACGACTTCTCCGATTGCGCCTCCGACTACTGAACCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
7003R-2.in silico     181 AAATGGAGGTAACCAAGTCGGAGGACGACTTCTCCGATTGCGCCTCCGACTAC-GAACCG 240


7003R-2.IR_full       241 GATGAGAATGAAGCCAGCGATGATAGCGTTAGNAGCGGCGCGGNAAGGAGGTNGTCGCCT 300
                          |||||||||||||||||||||||||||||||| ||||||||||  ||||||| |||||||
7003R-2.in silico     241 GATGAGAATGAAGCCAGCGATGATAGCGTTAGCAGCGGCGCGG--AGGAGGT-GTCGCCT 300


7003R-2.IR_full       301 AGCGAGAACGACATGTCCGTGGACAGTCCGACACCCAAGAAATCGCGCAAAAAGTCTAAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7003R-2.in silico     301 AGCGAGAACGACATGTCCGTGGACAGTCCGACACCCAAGAAATCGCGCAAAAAGTCTAAG 360


7003R-2.IR_full       361 ATTCTGAATAACAACAATAACAATGGAGCCATCGAGCAAGAAGGTCAAGTCTAGAGTCGA 420
                          |||||||||||||||||||||||| |||||||||||||||||||||||| ||||||||||
7003R-2.in silico     361 ATTCTGAATAACAACAATAACAAT-GAGCCATCGAGCAAGAAGGTCAAG-CTAGAGTCGA 420


7003R-2.IR_full       421 CCATCCAGTTGGCCGAGGGNNTGCAACTCTTTCAAGAAAAGCTGAAGAACCTGCAGAGCA 480
                          |||||||||||||||||||  |||||| ||||||||||||||||||||||||||||||||
7003R-2.in silico     421 CCATCCAGTTGGCCGAGGG--TGCAAC-CTTTCAAGAAAAGCTGAAGAACCTGCAGAGCA 480


7003R-2.IR_full       481 ATGCCAAGCAGGATGCTTCCTACGATGAT 509
                          |||||||||||||||||||||||||||||
7003R-2.in silico     481 ATGCCAAGCAGGATGCTTCCTACGATGAT 509

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_140498.1	CG7003-RA (CG7003), mRNA
0.62	3	3	2	4	NM_139485.1	CG12187-RA (CG12187), mRNA
0.2	1	4	3	4	NM_132259.2	CG11219-RA (PIP82), mRNA
0	0	3	3	9	NM_139488.2	CG1240-RA (CG1240), mRNA
0	0	3	2	6	NM_143452.1	CG1911-RA (CAP-D2), mRNA
0	0	3	2	2	NM_206606.1	CG3806-RB, transcript variant B (eIF2B-epsilon), mRNA
0	0	3	2	2	NM_130605.3	CG3806-RA, transcript variant A (eIF2B-epsilon), mRNA
0	0	2	12	13	NM_078549.3	CG32688-RA, transcript variant A (Hk), mRNA
0	0	2	4	21	NM_167487.1	CG32577-RA (disco-r), mRNA
0	0	2	3	4	NM_143700.3	CG17724-RA, transcript variant A (CG17724), mRNA
0	0	2	3	3	NM_132213.1	CG2260-RA (CG2260), mRNA
0	0	2	2	5	NM_001014486.1	CG4952-RG, transcript variant G (dac), mRNA
0	0	2	2	5	NM_165159.1	CG4952-RC, transcript variant C (dac), mRNA
0	0	2	2	5	NM_165160.1	CG4952-RA, transcript variant A (dac), mRNA
0	0	2	2	5	NM_165162.1	CG4952-RB, transcript variant B (dac), mRNA
0	0	2	2	5	NM_165161.1	CG4952-RD, transcript variant D (dac), mRNA
0	0	2	2	5	NM_001014487.1	CG4952-RF, transcript variant F (dac), mRNA
0	0	2	2	5	NM_165163.1	CG4952-RE, transcript variant E (dac), mRNA
0	0	2	2	3	NM_079649.2	CG18740-RA (mor), mRNA
0	0	2	2	3	NM_057430.3	CG5271-RA (RpS27A), mRNA
0	0	2	2	3	NM_132606.1	CG3754-RA (CG3754), mRNA
0	0	2	2	2	NM_135088.2	CG6907-RA (CG6907), mRNA
0	0	1	8	9	NM_132413.1	CG15295-RA (CG15295), mRNA
0	0	1	8	8	NM_167222.1	CG32688-RB, transcript variant B (Hk), mRNA
0	0	1	6	9	NM_166230.1	CG4816-RA, transcript variant A (qkr54B), mRNA
0	0	1	6	9	NM_166231.1	CG4816-RC, transcript variant C (qkr54B), mRNA
0	0	1	6	9	NM_079048.2	CG4816-RB, transcript variant B (qkr54B), mRNA
0	0	1	5	5	NM_140597.1	CG13070-RA (CG13070), mRNA
0	0	1	4	14	NM_169588.1	CG31317-RA, transcript variant A (stumps), mRNA
0	0	1	4	14	NM_169589.1	CG31317-RB, transcript variant B (stumps), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance
+

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