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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6947R-3 
 Symbol CG6947  Full Name CG6947 
 CG No CG6947  Old CG No CG6947 
 Synonyms CG6947 
 Accession No (Link to NCBI) NM_140276.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Zahoor MK, Poidevin M, Lecerf C, Garrido D, Montagne J.<br> A Drosophila genetic screen for suppressors of S6kinase-dependent growth identifies the F-box subunit Archipelago/FBXW7.<br> Mol Genet Genomics (2019) 294(3) 573-582 [ PubMed ID = 30656413 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTACAACCCCAAGAATGGCAAG 
 Primer Seq. 3' 
aatctagaggtaccCACACATAGAACAAGGAGCAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TACAACCCCA AGAATGGCAA GTGCACAATG GCAGGACGAA GTCTCTGCAA GCTGAGTCTT 
0061 TTAGCTCCAC TCGCTGAGGC CACCAATGTG TGCTCCACCG AGGTGAACGG AGCGTACATA 
0121 GCCAATAGTG GTTCTTGTGG CGAGTTCTAC ATCTGCGACG AGCAGATTGC CTATCCACAG 
0181 AAGTGCGATT TAGGAAGCTT CTTCAATGAA ACCCTGGCCG CCTGCATACC GGATGCAAAC 
0241 TCCACGTGCT GGCAGAACCT GTGCATCAAT AAGACCAATG GTGTCTTTGT CGAAAATGAG 
0301 GCGAACTGCG GTAGCTACNT ACGTGTGCTC CAATGGAGAA GCCACTCTTC AGACTTGTCC 
0361 CCAAGGCAGC TTTTTCAACA CCTCAGTTGC CGCCTGTACT GTCGATCAAG GAAACTCACA 
0421 ATGCTGGGTT AACTACTGCA TTGGACAGGA TGATGGGTCC GCTGTGGCCG ACAAGTCCAA 
0481 CTGCTCCTTG TTCTATGTGT G 
 in silico PCR Fragment 
0001 TACAACCCCA AGAATGGCAA GTGCACAATG GCAGGACGAA GTCTCTGCAA GCTGAGTCTT 
0061 TTAGCTCCAC TCGCTGAGGC CACCAATGTG TGCTCCACCG AGGTGAACGG AGCGTACATA 
0121 GCCAATAGTG GTTCTTGTGG CGAGTTCTAC ATCTGCGACG AGCAGATTGC CTATCCACAG 
0181 AAGTGCGATT TAGGAAGCTT CTTCAATGAA ACCCTGGCCG CCTGCATACC GGATGCAAAC 
0241 TCCACGTGCT GGCAGAACCT GTGCATCAAT AAGACCAATG GTGTCTTTGT CGAAAATGAG 
0301 GCGAACTGCG GTAGCTAC-T ACGTGTGCTC CAATGGAGAA GCCACTCTTC AGACTTGTCC 
0361 CCAAGGCAGC TTTTTCAACA CCTCAGTTGC CGCCTGTACT GTCGATCAAG GAAACTCACA 
0421 ATGCTGGGTT AACTACTGCA TTGGACAGGA TGATGGGTCC GCTGTGGCCG ACAAGTCCAA 
0481 CTGCTCCTTG TTCTATGTGT G 
 Assemble Data 
<PRE>

6947R-3.IR_full       1   TACAACCCCAAGAATGGCAAGTGCACAATGGCAGGACGAAGTCTCTGCAAGCTGAGTCTT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     1   TACAACCCCAAGAATGGCAAGTGCACAATGGCAGGACGAAGTCTCTGCAAGCTGAGTCTT 60


6947R-3.IR_full       61  TTAGCTCCACTCGCTGAGGCCACCAATGTGTGCTCCACCGAGGTGAACGGAGCGTACATA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     61  TTAGCTCCACTCGCTGAGGCCACCAATGTGTGCTCCACCGAGGTGAACGGAGCGTACATA 120


6947R-3.IR_full       121 GCCAATAGTGGTTCTTGTGGCGAGTTCTACATCTGCGACGAGCAGATTGCCTATCCACAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     121 GCCAATAGTGGTTCTTGTGGCGAGTTCTACATCTGCGACGAGCAGATTGCCTATCCACAG 180


6947R-3.IR_full       181 AAGTGCGATTTAGGAAGCTTCTTCAATGAAACCCTGGCCGCCTGCATACCGGATGCAAAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     181 AAGTGCGATTTAGGAAGCTTCTTCAATGAAACCCTGGCCGCCTGCATACCGGATGCAAAC 240


6947R-3.IR_full       241 TCCACGTGCTGGCAGAACCTGTGCATCAATAAGACCAATGGTGTCTTTGTCGAAAATGAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     241 TCCACGTGCTGGCAGAACCTGTGCATCAATAAGACCAATGGTGTCTTTGTCGAAAATGAG 300


6947R-3.IR_full       301 GCGAACTGCGGTAGCTAC<span class="snp"><tt>N</tt></span>TACGTGTGCTCCAATGGAGAAGCCACTCTTCAGACTTGTCC 360
                          ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     301 GCGAACTGCGGTAGCTAC<span class="snp"><tt>-</tt></span>TACGTGTGCTCCAATGGAGAAGCCACTCTTCAGACTTGTCC 360


6947R-3.IR_full       361 CCAAGGCAGCTTTTTCAACACCTCAGTTGCCGCCTGTACTGTCGATCAAGGAAACTCACA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     361 CCAAGGCAGCTTTTTCAACACCTCAGTTGCCGCCTGTACTGTCGATCAAGGAAACTCACA 420


6947R-3.IR_full       421 ATGCTGGGTTAACTACTGCATTGGACAGGATGATGGGTCCGCTGTGGCCGACAAGTCCAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-3.in silico     421 ATGCTGGGTTAACTACTGCATTGGACAGGATGATGGGTCCGCTGTGGCCGACAAGTCCAA 480


6947R-3.IR_full       481 CTGCTCCTTGTTCTATGTGTG 501
                          |||||||||||||||||||||
6947R-3.in silico     481 CTGCTCCTTGTTCTATGTGTG 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  1  4  NM_140276.2  CG6947-RA (CG6947), mRNA 
0   0  3  3  2  NM_167078.1  CG17717-RB, transcript variant B (CG17717), mRNA 
0   0  3  3  2  NM_132099.2  CG17717-RA, transcript variant A (CG17717), mRNA 
0   0  1  4  5  NM_142786.1  CG13847-RA (CG13847), mRNA 
0   0  1  3  9  NM_001043114.1  CG33484-RD, transcript variant D (zormin), mRNA 
0   0  1  3  9  NM_001043112.1  CG33484-RB, transcript variant B (zormin), mRNA 
0   0  1  3  9  NM_206240.1  CG33484-RA, transcript variant A (zormin), mRNA 
0   0  1  3  5  NM_143449.1  CG18110-RA (CG18110), mRNA 
0   0  1  3  3  NM_134534.2  CG17052-RA (CG17052), mRNA 
0   0  1  3  2  NM_137269.2  CG7747-RA (CG7747), mRNA 
0   0  1  2  5  NM_164375.1  CG18497-RC, transcript variant C (spen), mRNA 
0   0  1  2  5  NM_164374.1  CG18497-RA, transcript variant A (spen), mRNA 
0   0  1  2  5  NM_079979.2  CG18497-RB, transcript variant B (spen), mRNA 
0   0  1  2  4  NM_001014521.1  CG30048-RB, transcript variant B (CG30048), mRNA 
0   0  1  2  4  NM_165895.3  CG30048-RA, transcript variant A (CG30048), mRNA 
0   0  1  2  3  NM_079567.2  CG18802-RA (alpha-Man-II), mRNA 
0   0  1  2  3  NM_001038955.1  CG33959-RC, transcript variant C (Octbeta3R), mRNA 
0   0  1  2  3  NM_001038956.1  CG33959-RE, transcript variant E (Octbeta3R), mRNA 
0   0  1  2  3  NM_001038957.1  CG33959-RB, transcript variant B (Octbeta3R), mRNA 
0   0  1  2  3  NM_001038954.1  CG33959-RD, transcript variant D (Octbeta3R), mRNA 
0   0  1  2  3  NM_001038958.1  CG33959-RA, transcript variant A (Octbeta3R), mRNA 
0   0  1  2  2  NM_165340.1  CG9317-RB, transcript variant B (CG9317), mRNA 
0   0  1  2  2  NM_136208.2  CG9317-RA, transcript variant A (CG9317), mRNA 
0   0  0  6  5  NM_057992.2  CG5562-RA (gbb), mRNA 
0   0  0  5  5  NM_057251.3  CG3647-RB, transcript variant B (stc), mRNA 
0   0  0  5  5  NM_057250.3  CG3647-RA, transcript variant A (stc), mRNA 
0   0  0  4  3  NM_141027.2  CG10565-RA (CG10565), mRNA 
0   0  0  4  3  NM_143245.2  CG14247-RA (CG14247), mRNA 
0   0  0  4  3  NM_168404.1  CG32056-RB, transcript variant B (CG32056), mRNA 
0   0  0  4  3  NM_168403.1  CG32056-RC, transcript variant C (CG32056), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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