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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6947R-1 |
| Symbol |
CG6947 |
Full Name |
CG6947 |
| CG No |
CG6947 |
Old CG No |
CG6947 |
| Synonyms |
CG6947 |
| Accession No (Link to NCBI) |
NM_140276.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTACAACCCCAAGAATGGCAAG |
| Primer Seq. 3' |
aatctagaggtaccCACACATAGAACAAGGAGCAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TACAACCCCA AGAATGGCAA GTGCACAATG GCAGGACGAA GTCTCTGCAA GCTGAGTCTT
0061 TTAGCTCCAC TCGCTGAGGC CACCAATGTG TGCTCCACCG AGGTGAACGG AGCGTACATA
0121 GCCAATAGTG GTTCTTGTGG CGAGTTCTAC ATCTGCGACG AGCAGATTGC CTATCCACAG
0181 AAGTGCGATT TAGGAAGCTT CTTCAATGAA ACCCTGGCCG CCTGCATACC GGATGCAAAC
0241 TCCACGTGCT GGCAGAACCT GTGCATCAAT AAGACCAATG GTGTCTTTGT CGAAAATGAG
0301 GCGAACTGCG GTAGCTACNT ACGTGTGCTC CAATGGAGAA GCCACTCTTC AGACTTGTCC
0361 CCAAGGCAGC TTTTTCAACA CCTCAGTTGC CGCCTGTACT GTCGATCAAG GAAACTCACA
0421 ATGCTGGGTT AACTACTGCA TTGGACAGGA TGATGGGTCC GCTGTGGCCG ACAAGTCCAA
0481 CTGCTCCTTG TTCTATGTGT G |
| in silico PCR Fragment |
0001 TACAACCCCA AGAATGGCAA GTGCACAATG GCAGGACGAA GTCTCTGCAA GCTGAGTCTT
0061 TTAGCTCCAC TCGCTGAGGC CACCAATGTG TGCTCCACCG AGGTGAACGG AGCGTACATA
0121 GCCAATAGTG GTTCTTGTGG CGAGTTCTAC ATCTGCGACG AGCAGATTGC CTATCCACAG
0181 AAGTGCGATT TAGGAAGCTT CTTCAATGAA ACCCTGGCCG CCTGCATACC GGATGCAAAC
0241 TCCACGTGCT GGCAGAACCT GTGCATCAAT AAGACCAATG GTGTCTTTGT CGAAAATGAG
0301 GCGAACTGCG GTAGCTAC-T ACGTGTGCTC CAATGGAGAA GCCACTCTTC AGACTTGTCC
0361 CCAAGGCAGC TTTTTCAACA CCTCAGTTGC CGCCTGTACT GTCGATCAAG GAAACTCACA
0421 ATGCTGGGTT AACTACTGCA TTGGACAGGA TGATGGGTCC GCTGTGGCCG ACAAGTCCAA
0481 CTGCTCCTTG TTCTATGTGT G |
| Assemble Data |
<PRE>
6947R-1.IR_full 1 TACAACCCCAAGAATGGCAAGTGCACAATGGCAGGACGAAGTCTCTGCAAGCTGAGTCTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 1 TACAACCCCAAGAATGGCAAGTGCACAATGGCAGGACGAAGTCTCTGCAAGCTGAGTCTT 60
6947R-1.IR_full 61 TTAGCTCCACTCGCTGAGGCCACCAATGTGTGCTCCACCGAGGTGAACGGAGCGTACATA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 61 TTAGCTCCACTCGCTGAGGCCACCAATGTGTGCTCCACCGAGGTGAACGGAGCGTACATA 120
6947R-1.IR_full 121 GCCAATAGTGGTTCTTGTGGCGAGTTCTACATCTGCGACGAGCAGATTGCCTATCCACAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 121 GCCAATAGTGGTTCTTGTGGCGAGTTCTACATCTGCGACGAGCAGATTGCCTATCCACAG 180
6947R-1.IR_full 181 AAGTGCGATTTAGGAAGCTTCTTCAATGAAACCCTGGCCGCCTGCATACCGGATGCAAAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 181 AAGTGCGATTTAGGAAGCTTCTTCAATGAAACCCTGGCCGCCTGCATACCGGATGCAAAC 240
6947R-1.IR_full 241 TCCACGTGCTGGCAGAACCTGTGCATCAATAAGACCAATGGTGTCTTTGTCGAAAATGAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 241 TCCACGTGCTGGCAGAACCTGTGCATCAATAAGACCAATGGTGTCTTTGTCGAAAATGAG 300
6947R-1.IR_full 301 GCGAACTGCGGTAGCTAC<span class="snp"><tt>N</tt></span>TACGTGTGCTCCAATGGAGAAGCCACTCTTCAGACTTGTCC 360
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 301 GCGAACTGCGGTAGCTAC<span class="snp"><tt>-</tt></span>TACGTGTGCTCCAATGGAGAAGCCACTCTTCAGACTTGTCC 360
6947R-1.IR_full 361 CCAAGGCAGCTTTTTCAACACCTCAGTTGCCGCCTGTACTGTCGATCAAGGAAACTCACA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 361 CCAAGGCAGCTTTTTCAACACCTCAGTTGCCGCCTGTACTGTCGATCAAGGAAACTCACA 420
6947R-1.IR_full 421 ATGCTGGGTTAACTACTGCATTGGACAGGATGATGGGTCCGCTGTGGCCGACAAGTCCAA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6947R-1.in silico 421 ATGCTGGGTTAACTACTGCATTGGACAGGATGATGGGTCCGCTGTGGCCGACAAGTCCAA 480
6947R-1.IR_full 481 CTGCTCCTTGTTCTATGTGTG 501
|||||||||||||||||||||
6947R-1.in silico 481 CTGCTCCTTGTTCTATGTGTG 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
4 |
NM_140276.2 |
CG6947-RA (CG6947), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_167078.1 |
CG17717-RB, transcript variant B (CG17717), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_132099.2 |
CG17717-RA, transcript variant A (CG17717), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_142786.1 |
CG13847-RA (CG13847), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_143449.1 |
CG18110-RA (CG18110), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_134534.2 |
CG17052-RA (CG17052), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137269.2 |
CG7747-RA (CG7747), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_164375.1 |
CG18497-RC, transcript variant C (spen), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_164374.1 |
CG18497-RA, transcript variant A (spen), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_079979.2 |
CG18497-RB, transcript variant B (spen), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014521.1 |
CG30048-RB, transcript variant B (CG30048), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165895.3 |
CG30048-RA, transcript variant A (CG30048), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079567.2 |
CG18802-RA (alpha-Man-II), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001038955.1 |
CG33959-RC, transcript variant C (Octbeta3R), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001038956.1 |
CG33959-RE, transcript variant E (Octbeta3R), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001038957.1 |
CG33959-RB, transcript variant B (Octbeta3R), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001038954.1 |
CG33959-RD, transcript variant D (Octbeta3R), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001038958.1 |
CG33959-RA, transcript variant A (Octbeta3R), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165340.1 |
CG9317-RB, transcript variant B (CG9317), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136208.2 |
CG9317-RA, transcript variant A (CG9317), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_057992.2 |
CG5562-RA (gbb), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_057251.3 |
CG3647-RB, transcript variant B (stc), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_057250.3 |
CG3647-RA, transcript variant A (stc), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_141027.2 |
CG10565-RA (CG10565), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_143245.2 |
CG14247-RA (CG14247), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_168404.1 |
CG32056-RB, transcript variant B (CG32056), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_168403.1 |
CG32056-RC, transcript variant C (CG32056), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| +- |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| |
|
| DB |
|
| + |
|
| DC |
|
| + |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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|
