NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6928R-3

Symbol

CG6928

Full Name

CG6928

CG No

CG6928

Old CG No

CG6928

Synonyms

CG6928

Accession No (Link to NCBI)

NM_168486.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATGCAGTTGCGGATCTGATA

Primer Seq. 3'

aatctagaggtaccCGGATTCGACCATCCAGCTTA

Predicted Fragment Size

IR fragment full Seq

0001 GATGCAGTTG CGGATCTGAT AGCCGGAGTG ACGGTCGGCC TGACGGTGTT GCCCCAAGGA 
0061 CTGGCCTACG CTACGCTGGC GGGACTGGAG CCCCAATATG GTCTGTACTC CGCCTTTGTG 
0121 GGCGGTATTA TATACGCCAT GCTGGGCAGC TGCCGGCAGG TGACCATTGG ACCCACGGCT 
0181 TTGCTCGCAC TGATGACAAG CCGGCACACG GGATTTGGAC TGGGATCGGG TCCAGCGTAC 
0241 GCGAGTACTT TTGTGCCTCA TCTCTGGTGT TGTGGAGCTG GGTATGGACA GTGCTGAAAC 
0301 TGGGTGCCCT GGGTGGACCT GATATCACTG CCAGTTACCG TGGGATTCAC CTCGGCCACC 
0361 GCCGTCATCA TTGGCACCTC GGCAGCTAAA GGGTTTGCTG GGACTGGCGT GGATGGATCC 
0421 GGGTCTGATT TCATCAATAC CATGCGCTCC GTGTTCGGAA AATCTACACA AAGTTAGAAC 
0481 TGGTGATTTT ACCCTTGGTT TGACTTCCAT TATAGTTTTG CTACTTCTGC GGAAACTAAA 
0541 AGACGTTAAG CTGGATGGTC GAATCCG

in silico PCR Fragment

0001 GATGCAGTTG CGGATCTGAT AGCCGGAGTG ACGGTCGGCC TGACGGTGTT GCCCCAAGGA 
0061 CTGGCCTACG CTACGCTGGC GGGACTGGAG CCCCAATATG GTCTGTACTC CGCCTTTGTG 
0121 GGCGGTATTA TATACGCCAT GCTGGGCAGC TGCCGGCAGG TGACCATTGG ACCCACGGCT 
0181 TTGCTCGCAC TGATGACAAG CCGGCACACG GGATTTGGAC TGGGATCGGG TCCAGCGTAC 
0241 GCGA-TACTT TTGTGCCTCA TCTCTGGTGT TGTGGAGCTG GGTATGG-CA GTGCTGAAAC 
0301 TGGGTGCCCT -GGTGGACCT GATATCACTG CCAGTTACCG TGGGATTCAC CTCGGCCACC 
0361 GCCGTCATCA TTGGCACCTC -GCAGCTAAA GGGTTTGCTG GGACT-GCGT GG-TGGATCC 
0421 GGGTCTGATT TCATCAATAC CATGCGCTCC GTGTTCGG-A AATCTACACA AAGTTAGAAC 
0481 TGGTGATTTT ACCCTTGGTT TGACTTCCAT TATAGTTTTG CTACTTCTGC GGAAACTAAA 
0541 AGACGTTAAG CTGGATGGTC GAATCCG

Assemble Data

<PRE>

6928R-3.IR_full       1   GATGCAGTTGCGGATCTGATAGCCGGAGTGACGGTCGGCCTGACGGTGTTGCCCCAAGGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6928R-3.in silico     1   GATGCAGTTGCGGATCTGATAGCCGGAGTGACGGTCGGCCTGACGGTGTTGCCCCAAGGA 60


6928R-3.IR_full       61  CTGGCCTACGCTACGCTGGCGGGACTGGAGCCCCAATATGGTCTGTACTCCGCCTTTGTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6928R-3.in silico     61  CTGGCCTACGCTACGCTGGCGGGACTGGAGCCCCAATATGGTCTGTACTCCGCCTTTGTG 120


6928R-3.IR_full       121 GGCGGTATTATATACGCCATGCTGGGCAGCTGCCGGCAGGTGACCATTGGACCCACGGCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6928R-3.in silico     121 GGCGGTATTATATACGCCATGCTGGGCAGCTGCCGGCAGGTGACCATTGGACCCACGGCT 180


6928R-3.IR_full       181 TTGCTCGCACTGATGACAAGCCGGCACACGGGATTTGGACTGGGATCGGGTCCAGCGTAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6928R-3.in silico     181 TTGCTCGCACTGATGACAAGCCGGCACACGGGATTTGGACTGGGATCGGGTCCAGCGTAC 240


6928R-3.IR_full       241 GCGA<span class="snp"><tt>G</tt></span>TACTTTTGTGCCTCATCTCTGGTGTTGTGGAGCTGGGTATGG<span class="snp"><tt>A</tt></span>CAGTGCTGAAAC 300
                          ||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
6928R-3.in silico     241 GCGA<span class="snp"><tt>-</tt></span>TACTTTTGTGCCTCATCTCTGGTGTTGTGGAGCTGGGTATGG<span class="snp"><tt>-</tt></span>CAGTGCTGAAAC 300


6928R-3.IR_full       301 TGGGTGCCCT<span class="snp"><tt>G</tt></span>GGTGGACCTGATATCACTGCCAGTTACCGTGGGATTCACCTCGGCCACC 360
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
6928R-3.in silico     301 TGGGTGCCCT<span class="snp"><tt>-</tt></span>GGTGGACCTGATATCACTGCCAGTTACCGTGGGATTCACCTCGGCCACC 360


6928R-3.IR_full       361 GCCGTCATCATTGGCACCTC<span class="snp"><tt>G</tt></span>GCAGCTAAAGGGTTTGCTGGGACT<span class="snp"><tt>G</tt></span>GCGTGG<span class="snp"><tt>A</tt></span>TGGATCC 420
                          ||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
6928R-3.in silico     361 GCCGTCATCATTGGCACCTC<span class="snp"><tt>-</tt></span>GCAGCTAAAGGGTTTGCTGGGACT<span class="snp"><tt>-</tt></span>GCGTGG<span class="snp"><tt>-</tt></span>TGGATCC 420


6928R-3.IR_full       421 GGGTCTGATTTCATCAATACCATGCGCTCCGTGTTCGG<span class="snp"><tt>A</tt></span>AAATCTACACAAAGTTAGAAC 480
                          ||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
6928R-3.in silico     421 GGGTCTGATTTCATCAATACCATGCGCTCCGTGTTCGG<span class="snp"><tt>-</tt></span>AAATCTACACAAAGTTAGAAC 480


6928R-3.IR_full       481 TGGTGATTTTACCCTTGGTTTGACTTCCATTATAGTTTTGCTACTTCTGCGGAAACTAAA 540
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6928R-3.in silico     481 TGGTGATTTTACCCTTGGTTTGACTTCCATTATAGTTTTGCTACTTCTGCGGAAACTAAA 540


6928R-3.IR_full       541 AGACGTTAAGCTGGATGGTCGAATCCG 567
                          |||||||||||||||||||||||||||
6928R-3.in silico     541 AGACGTTAAGCTGGATGGTCGAATCCG 567

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	542	0	0	4	NM_140282.2	CG6928-RB, transcript variant B (CG6928), mRNA
100	542	0	0	4	NM_168486.1	CG6928-RA, transcript variant A (CG6928), mRNA
0	0	3	2	2	NM_142395.1	CG7431-RA (CG7431), mRNA
0	0	2	4	11	NM_142208.2	CG6125-RB, transcript variant B (CG6125), mRNA
0	0	2	4	11	NM_169656.1	CG6125-RA, transcript variant A (CG6125), mRNA
0	0	2	4	3	NM_135977.1	CG15133-RA (CG15133), mRNA
0	0	2	3	3	NM_168601.1	CG3297-RC, transcript variant C (mnd), mRNA
0	0	2	3	3	NM_168600.1	CG3297-RB, transcript variant B (mnd), mRNA
0	0	2	3	3	NM_079350.4	CG3297-RA, transcript variant A (mnd), mRNA
0	0	1	4	11	NM_137418.2	CG5002-RA (CG5002), mRNA
0	0	1	3	3	NM_142221.1	CG18516-RA (CG18516), mRNA
0	0	1	3	2	NM_141216.2	CG14650-RA (CG14650), mRNA
0	0	1	2	5	NM_132173.2	CG12153-RA (Hira), mRNA
0	0	1	2	5	NM_138010.3	CG16783-RA (fzr2), mRNA
0	0	1	2	3	NM_142504.2	CG17836-RB, transcript variant B (CG17836), mRNA
0	0	1	2	3	NM_133012.2	CG32560-RA (CG32560), mRNA
0	0	0	6	3	NM_143061.1	CG13648-RA (tnc), mRNA
0	0	0	5	6	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	0	5	5	NM_169057.1	CG31547-RB, transcript variant B (CG31547), mRNA
0	0	0	5	5	NM_169058.1	CG31547-RA, transcript variant A (CG31547), mRNA
0	0	0	5	4	NM_001015316.1	CG17514-PA.3 (CG17514), mRNA
0	0	0	5	2	NM_001038712.1	CG33978-RA (CG33978), mRNA
0	0	0	5	2	NM_167879.1	CG32331-RA (CG32331), mRNA
0	0	0	4	12	NM_166021.1	CG6671-RC, transcript variant C (AGO1), mRNA
0	0	0	4	12	NM_166020.1	CG6671-RA, transcript variant A (AGO1), mRNA
0	0	0	4	11	NM_079010.2	CG6671-RB, transcript variant B (AGO1), mRNA
0	0	0	4	7	NM_138045.2	CG4057-RB, transcript variant B (tamo), mRNA
0	0	0	4	7	NM_166664.1	CG4057-RA, transcript variant A (tamo), mRNA
0	0	0	4	4	NM_141308.1	CG12170-RA (CG12170), mRNA
0	0	0	4	4	NM_206691.2	CG1817-RA, transcript variant A (Ptp10D), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Pathways (updated: 2024/04/26)

KEGG Pathway

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