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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6896R-2

Symbol MYPT-75D Full Name MYPT-75D

CG No CG6896 Old CG No CG6896

Synonyms CG6896, MYPT-75D

Accession No (Link to NCBI) NM_140792.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCAGGAGGATGCCAGCTACA

Primer Seq. 3'

aatctagaggtaccATTCGCACCGTACTCCAGCAG

Predicted Fragment Size

IR fragment full Seq

0001 NCCATGGGAG GATGCCAGCT ACACCAAGCA GCTCAAGATG GAGCACGCCG ATCTGGTGGC 
0061 CGAGATGCAG ACCGTGGAGA GCCTGCCCAC CCACGAGCGA CTCCAGCTGG CCAGACTACG 
0121 TCGTGCGCAG CAGCTGNAAG CTGTCCCGCC AGAAGGACAA GGAGTGGGCC AAGTTGCAGC 
0181 GGGCGAAGGG CNAACACGGC GAGCGGCGGC AGCGGAACCG GATCGCTGGG CAACGGGCTG 
0241 ATGAACGGCA ACGGAGATAC CACGCACCAC TTCCGGTACG CCAATGGTTC GGGCACGTTG 
0301 AGCGGCAGGC GGCACATATC CTTCGAGAAC AGCGTGGTGC TCCTGGAGGC GGCATCCCGG 
0361 AACGACATGC CCGAGGTGGC CGCCCTGCTG CAGCACGGAA TCACGCCGGA TGCCGCAAAC 
0421 GAGGATGGAC TGACGGCGAT GCATCAGGCG TGCATCGATA ACAACGTCGA GATGCTGCAG 
0481 CTGCTGCTGG AGTACGGTGC GAAT

in silico PCR Fragment

0001 GCCA--GGAG GATGCCAGCT ACACCAAGCA GCTCAAGATG GAGCACGCCG ATCTGGTGGC 
0061 CGAGATGCAG ACCGTGGAGA GCCTGCCCAC CCACGAGCGA CTCCAGCTGG CCAGACTACG 
0121 TCGTGCGCAG CAGCTG-AAG CTGTCCCGCC AGAAGGACAA GGAGTGGGCC AAGTTGCAGC 
0181 GGGCGAAGGG C-AACACGGC GAGCGGCGGC AGCGGAACCG GATCGCTGGG CAACGGGCTG 
0241 ATGAACGGCA ACGGAGATAC CACGCACCAC TTCCGGCACG CCAATGGTTC GGGCACGTTG 
0301 AGCGGCAGGC GGCACATATC CTTCGAGAAC AGCGTGGTGC TCCTGGAGGC GGCATCCCGG 
0361 AACGACATGC CCGAGGTGGC CGCCCTGCTG CAGCACGGAA TCACGCCGGA TGCCGCAAAC 
0421 GAGGATGGAC TGACGGCGAT GCATCAGGCG TGCATCGATA ACAACGTCGA GATGCTGCAG 
0481 CTGCTGCTGG AGTACGGTGC GAAT

Assemble Data


6896R-2.IR_full        1   CCATGGGAGGATGCCAGCTACACCAAGCAGCTCAAGATGGAGCACGCCGATCTGGTGGC 59
                           |||  ||||||||||||||||||||||||||||||||||||||||||||||||||||||
6896R-2.in silico      1   CCA--GGAGGATGCCAGCTACACCAAGCAGCTCAAGATGGAGCACGCCGATCTGGTGGC 59


6896R-2.IR_full       61  CGAGATGCAGACCGTGGAGAGCCTGCCCACCCACGAGCGACTCCAGCTGGCCAGACTACG 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6896R-2.in silico     61  CGAGATGCAGACCGTGGAGAGCCTGCCCACCCACGAGCGACTCCAGCTGGCCAGACTACG 119


6896R-2.IR_full       121 TCGTGCGCAGCAGCTGNAAGCTGTCCCGCCAGAAGGACAAGGAGTGGGCCAAGTTGCAGC 179
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
6896R-2.in silico     121 TCGTGCGCAGCAGCTG-AAGCTGTCCCGCCAGAAGGACAAGGAGTGGGCCAAGTTGCAGC 179


6896R-2.IR_full       181 GGGCGAAGGGCNAACACGGCGAGCGGCGGCAGCGGAACCGGATCGCTGGGCAACGGGCTG 239
                          ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
6896R-2.in silico     181 GGGCGAAGGGC-AACACGGCGAGCGGCGGCAGCGGAACCGGATCGCTGGGCAACGGGCTG 239


6896R-2.IR_full       241 ATGAACGGCAACGGAGATACCACGCACCACTTCCGGTACGCCAATGGTTCGGGCACGTTG 299
                          |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
6896R-2.in silico     241 ATGAACGGCAACGGAGATACCACGCACCACTTCCGGCACGCCAATGGTTCGGGCACGTTG 299


6896R-2.IR_full       301 AGCGGCAGGCGGCACATATCCTTCGAGAACAGCGTGGTGCTCCTGGAGGCGGCATCCCGG 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6896R-2.in silico     301 AGCGGCAGGCGGCACATATCCTTCGAGAACAGCGTGGTGCTCCTGGAGGCGGCATCCCGG 359


6896R-2.IR_full       361 AACGACATGCCCGAGGTGGCCGCCCTGCTGCAGCACGGAATCACGCCGGATGCCGCAAAC 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6896R-2.in silico     361 AACGACATGCCCGAGGTGGCCGCCCTGCTGCAGCACGGAATCACGCCGGATGCCGCAAAC 419


6896R-2.IR_full       421 GAGGATGGACTGACGGCGATGCATCAGGCGTGCATCGATAACAACGTCGAGATGCTGCAG 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6896R-2.in silico     421 GAGGATGGACTGACGGCGATGCATCAGGCGTGCATCGATAACAACGTCGAGATGCTGCAG 479


6896R-2.IR_full       481 CTGCTGCTGGAGTACGGTGCGAAT 503
                          ||||||||||||||||||||||||
6896R-2.in silico     481 CTGCTGCTGGAGTACGGTGCGAAT 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	9	45	NM_140792.1	CG6896-RA (MYPT-75D), mRNA
0.2	1	2	7	37	NM_206622.2	CG32776-RB, transcript variant B (CG32776), mRNA
0.2	1	2	7	37	NM_001038740.1	CG32776-RD, transcript variant D (CG32776), mRNA
0.2	1	2	5	8	NM_141658.3	CG8301-RA (CG8301), mRNA
0	0	6	17	74	NM_132246.2	CG10555-RA (CG10555), mRNA
0	0	6	17	53	NM_132235.2	CG32717-RB, transcript variant B (sdt), mRNA
0	0	5	12	25	NM_001038747.1	CG32717-RF, transcript variant F (sdt), mRNA
0	0	5	9	42	NM_206653.1	CG32717-RE, transcript variant E (sdt), mRNA
0	0	5	9	42	NM_132236.3	CG32717-RA, transcript variant A (sdt), mRNA
0	0	5	9	20	NM_132115.1	CG14440-RA (CG14440), mRNA
0	0	5	5	20	NM_057385.4	CG11121-RA (so), mRNA
0	0	5	3	3	NM_134768.3	CG17660-RA (CG17660), mRNA
0	0	4	17	63	NM_057678.3	CG31240-RA (repo), mRNA
0	0	4	13	37	NM_001032408.1	CG33956-RD, transcript variant D (kay), mRNA
0	0	4	11	22	NM_136910.1	CG13165-RA (CG13165), mRNA
0	0	4	9	51	NM_143409.1	CG11873-RA (CG11873), mRNA
0	0	4	8	59	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	4	8	35	NM_206514.1	CG14307-RK, transcript variant K (fru), mRNA
0	0	4	8	35	NM_169818.1	CG14307-RH, transcript variant H (fru), mRNA
0	0	4	8	35	NM_169817.1	CG14307-RG, transcript variant G (fru), mRNA
0	0	4	8	30	NM_168361.1	CG16711-RB, transcript variant B (CG16711), mRNA
0	0	4	8	30	NM_140090.1	CG16711-RA, transcript variant A (CG16711), mRNA
0	0	4	5	6	NM_132504.1	CG11695-RA (CG11695), mRNA
0	0	4	4	14	NM_001042798.1	CG32717-RH, transcript variant H (sdt), mRNA
0	0	3	26	64	NM_079218.2	CG10491-RA, transcript variant A (vn), mRNA
0	0	3	26	64	NM_001014565.1	CG10491-RB, transcript variant B (vn), mRNA
0	0	3	21	86	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0	0	3	21	86	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0	0	3	18	64	NM_166873.1	CG3638-RB, transcript variant B (CG3638), mRNA
0	0	3	18	64	NM_166872.1	CG3638-RD, transcript variant D (CG3638), mRNA

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