NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6885R-1

Symbol

CG6885

Full Name

CG6885

CG No

CG6885

Old CG No

CG6885

Synonyms

anon-WO0118547.731, CG6885

Accession No (Link to NCBI)

NM_140799.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGACATCGAGGATAACTTCCGT

Primer Seq. 3'

aatctagaggtaccAGTGGAACCTTCTGATGGAGC

Predicted Fragment Size

IR fragment full Seq

0001 GACATCGAGG ATAACTTCCG TGTTTTTCGC CATCAGTTTT TCGTCAACGG TGCAAGGAGA 
0061 GCGGATTGCA CCACCTGTGA AAGTCGAGTG CCAGTCAGCG AACCATACCA TCATCATTGG 
0121 CGAAATGATA TCGAGAACAA TAGAAGCCAT TGCATTCAAA TTGGTTCTGA TGAAAAGGAT 
0181 ATCCTGAAGA AGATCGAGGA CCAGGCCATC GAAGAGTTTA TCTTGTGTGA TGGAAGCATT 
0241 GCGGCCAGGA CCAACGACTT TCTTCTGGAT GCTGGGATGG ATGCAGTTNC CGCAACTGCT 
0301 GCGATTTTTA AGTTTTGGTA CAGAAAAATT GGAGACCACA GTTGGATTTT ACGTGGATGT 
0361 GAAAAAGGAG CGAATGTACT ACGAAAGCAG TCCGTTAAAT ATCGAAAACC ATTTCGATAT 
0421 TGGTGAGGCG GTGGACATGA TATTCTCCAT GCTGCTGGAG AAGATCAGCA ACTACGTGCT 
0481 GCTCCATCAG AAGGTTCCAC T

in silico PCR Fragment

0001 GACATCGAGG ATAACTTCCG TGTGTTTCGC CACCAGTTTT TCGTCAACGG TGCAAGGAGA 
0061 GCGGATTGCA CAACCTGTGA AAGTCGAGTG CCAGTCAGCG AACCATACCA TCATCATTGG 
0121 CGAAATGATA TCGAGAACAA TAGAAGCCAT TGCATTCAAA TTGGTTCTGA TGAAAAGGAT 
0181 ATCCTGAAGA GGATCGAGGA CCAGGCCATC GAAGAGTTTA TCTTGTGTGA TGGAAGCATT 
0241 GCGGCCAGGA CCAACGACTT TCTTCTGGAT GCTGGGATGG ATGCAGTT-C CGCAACTGCT 
0301 GCGATTTTTA AGTTTTGGTA CAGAAAAATT GGAGACCACA GTTGGATTTT ACGTGGATGT 
0361 GAAAAAGGAG CGAATGTACT ACGAAAGCAG TCCGTTAAAT ATCGAAAACC ATTTCGATAT 
0421 TGGTGAGGCG GTGGACATGA TATTCTCCAT GCTGCTGGAG AAGATCAGCA ACTACGTGCT 
0481 GCTCCATCAG AAGGTTCCAC T

Assemble Data

<PRE>

6885R-1.IR_full       1   GACATCGAGGATAACTTCCGTGT<span class="snp"><tt>T</tt></span>TTTCGCCA<span class="snp"><tt>T</tt></span>CAGTTTTTCGTCAACGGTGCAAGGAGA 60
                          |||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||
6885R-1.in silico     1   GACATCGAGGATAACTTCCGTGT<span class="snp"><tt>G</tt></span>TTTCGCCA<span class="snp"><tt>C</tt></span>CAGTTTTTCGTCAACGGTGCAAGGAGA 60


6885R-1.IR_full       61  GCGGATTGCAC<span class="snp"><tt>C</tt></span>ACCTGTGAAAGTCGAGTGCCAGTCAGCGAACCATACCATCATCATTGG 120
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
6885R-1.in silico     61  GCGGATTGCAC<span class="snp"><tt>A</tt></span>ACCTGTGAAAGTCGAGTGCCAGTCAGCGAACCATACCATCATCATTGG 120


6885R-1.IR_full       121 CGAAATGATATCGAGAACAATAGAAGCCATTGCATTCAAATTGGTTCTGATGAAAAGGAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6885R-1.in silico     121 CGAAATGATATCGAGAACAATAGAAGCCATTGCATTCAAATTGGTTCTGATGAAAAGGAT 180


6885R-1.IR_full       181 ATCCTGAAGA<span class="snp"><tt>A</tt></span>GATCGAGGACCAGGCCATCGAAGAGTTTATCTTGTGTGATGGAAGCATT 240
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
6885R-1.in silico     181 ATCCTGAAGA<span class="snp"><tt>G</tt></span>GATCGAGGACCAGGCCATCGAAGAGTTTATCTTGTGTGATGGAAGCATT 240


6885R-1.IR_full       241 GCGGCCAGGACCAACGACTTTCTTCTGGATGCTGGGATGGATGCAGTT<span class="snp"><tt>N</tt></span>CCGCAACTGCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||
6885R-1.in silico     241 GCGGCCAGGACCAACGACTTTCTTCTGGATGCTGGGATGGATGCAGTT<span class="snp"><tt>-</tt></span>CCGCAACTGCT 300


6885R-1.IR_full       301 GCGATTTTTAAGTTTTGGTACAGAAAAATTGGAGACCACAGTTGGATTTTACGTGGATGT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6885R-1.in silico     301 GCGATTTTTAAGTTTTGGTACAGAAAAATTGGAGACCACAGTTGGATTTTACGTGGATGT 360


6885R-1.IR_full       361 GAAAAAGGAGCGAATGTACTACGAAAGCAGTCCGTTAAATATCGAAAACCATTTCGATAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6885R-1.in silico     361 GAAAAAGGAGCGAATGTACTACGAAAGCAGTCCGTTAAATATCGAAAACCATTTCGATAT 420


6885R-1.IR_full       421 TGGTGAGGCGGTGGACATGATATTCTCCATGCTGCTGGAGAAGATCAGCAACTACGTGCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6885R-1.in silico     421 TGGTGAGGCGGTGGACATGATATTCTCCATGCTGCTGGAGAAGATCAGCAACTACGTGCT 480


6885R-1.IR_full       481 GCTCCATCAGAAGGTTCCACT 501
                          |||||||||||||||||||||
6885R-1.in silico     481 GCTCCATCAGAAGGTTCCACT 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140799.1	CG6885-RA (CG6885), mRNA
0	0	3	5	3	NM_143428.2	CG14514-RA (Brd8), mRNA
0	0	3	4	2	NM_134534.2	CG17052-RA (CG17052), mRNA
0	0	3	3	4	NM_080363.5	CG17213-RA (Gr33a), mRNA
0	0	2	5	9	NM_001043289.1	CG34100-RA, transcript variant A (mld), mRNA
0	0	2	4	2	NM_164944.1	CG31869-RA (CG31869), mRNA
0	0	1	5	5	NM_137085.2	CG13016-RA (CG13016), mRNA
0	0	1	4	7	NM_137704.2	CG9394-RA (CG9394), mRNA
0	0	1	4	4	NM_140842.1	CG9629-RA (CG9629), mRNA
0	0	1	4	3	NM_141731.1	CG3996-RA (CG3996), mRNA
0	0	1	4	3	NM_169049.1	CG12000-RB, transcript variant B (CG12000), mRNA
0	0	1	4	3	NM_141272.1	CG12000-RA, transcript variant A (CG12000), mRNA
0	0	1	2	4	NM_176223.2	CG30115-RD, transcript variant D (CG30115), mRNA
0	0	1	2	4	NM_141129.1	CG11523-RA (CG11523), mRNA
0	0	1	2	4	NM_166867.1	CG11642-RB, transcript variant B (TRAM), mRNA
0	0	1	2	4	NM_166866.1	CG11642-RA, transcript variant A (TRAM), mRNA
0	0	1	2	4	NM_166868.1	CG11642-RC, transcript variant C (TRAM), mRNA
0	0	1	2	3	NM_136200.2	CG2478-RA (CG2478), mRNA
0	0	1	2	2	NM_134917.2	CG15406-RA (CG15406), mRNA
0	0	1	2	2	NM_167969.1	CG32488-RA (CG32488), mRNA
0	0	0	6	4	NM_136732.1	CG12904-RA (CG12904), mRNA
0	0	0	5	6	NM_079120.2	CG4356-RB, transcript variant B (mAcR-60C), mRNA
0	0	0	5	6	NM_166668.1	CG4356-RA, transcript variant A (mAcR-60C), mRNA
0	0	0	5	3	NM_134735.1	CG4785-RA (CG4785), mRNA
0	0	0	4	10	NM_080535.2	CG9774-RA (rok), mRNA
0	0	0	4	6	NM_134517.2	CG15618-RA (CG15618), mRNA
0	0	0	4	5	NM_057656.2	CG12345-RA, transcript variant A (Cha), mRNA
0	0	0	4	5	NM_206517.1	CG12345-RB, transcript variant B (Cha), mRNA
0	0	0	4	5	NM_079149.3	CG17046-RA, transcript variant A (klar), mRNA
0	0	0	4	5	NM_001043111.1	CG17046-RB, transcript variant B (klar), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Pathways (updated: 2024/04/26)

KEGG Pathway

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