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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6854R-2 
 Symbol CG6854  Full Name CG6854 
 CG No CG6854  Old CG No CG6854 
 Synonyms anon-WO0172774.92, anon-WO0172774.90, CG6854 
 Accession No (Link to NCBI) NM_168606.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTAACTGGTGGCGTCATTAGTG 
 Primer Seq. 3' 
aatctagaggtaccCTCTCTCTTTACGCGGAACTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TAACTGGTGG CGTCATTAGT GGCGTGGGAA AAGGAGTGAT TGCCTCCTCG TTCGGAACGC 
0061 TTTTGAAATC CTGTGGTCTG GATGTAACCT CGATCAAGAT TGACCCCTAT ATCAATATAG 
0121 ATGCTGGAAC CTTTTCGCCT TATGAGCATG GCGAGNGTTT ACGTTTTGGA CGATGGCGCC 
0181 GAGGTGGATC TGGATCTGGG AAACTATGAA CGGTTTTTGG ATGTTACCCT GCATCGGGAC 
0241 AACAACATAA CCACCGGAAA AATTTACAAG TTGGTCATTG AGAAGGAGCG CACTGGCGAG 
0301 TACTTGGGCA AAACGGTTCA AGTTGTCCCA CACATCACTG ATGCCATTCA GGAATGGGTG 
0361 GANGCGCGTG GCCCAGACAC CCGTTCAGGG ATCTTCAAAG CCACAGGTGT GCATCGTGGA 
0421 ATTGGGAGGA ACGATTGGTG ACATCGAAGG CATGCCTTTC GTAGAGGCCT TCCGTCAGTT 
0481 TCAGTTCCGC GTAAAGAGAG AG 
 in silico PCR Fragment 
0001 TAACTGGTGG CGTCATTAGT GGCGTGGGAA AAGGAGTGAT TGCCTCCTCG TTCGGAACGC 
0061 TTTTGAAATC CTGTGGTCTG GATGTAACCT CGATCAAGAT TGACCCCTAT ATCAATATAG 
0121 ATGCTGGAAC CTTTTCGCCT TATGAGCATG GCGAG-GTTT ACGTTTTGGA CGATGGCGCC 
0181 GAGGTGGATC TGGATCTGGG AAACTATGAA CGGTTTTTGG ATGTTACCCT GCATCGGGAC 
0241 AACAACATAA CCACCGGAAA AATTTACAAG TTGGTCATTG AGAAGGAGCG CACTGGCGAG 
0301 TACTTGGGCA AAACGGTTCA AGTTGTCCCA CACATCACTG ATGCCATTCA GGAATGGGTG 
0361 GA-GCGCGTG GCCCAGACAC CCGTTCAGGG ATCTTCAAAG CCACAGGTGT GCATCGTGGA 
0421 ATTGGGAGGA ACGATTGGTG ACATCGAAGG CATGCCTTTC GTAGAGGCCT TCCGTCAGTT 
0481 TCAGTTCCGC GTAAAGAGAG AG 
 Assemble Data 
<PRE>

6854R-2.IR_full       1   TAACTGGTGGCGTCATTAGTGGCGTGGGAAAAGGAGTGATTGCCTCCTCGTTCGGAACGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6854R-2.in silico     1   TAACTGGTGGCGTCATTAGTGGCGTGGGAAAAGGAGTGATTGCCTCCTCGTTCGGAACGC 60


6854R-2.IR_full       61  TTTTGAAATCCTGTGGTCTGGATGTAACCTCGATCAAGATTGACCCCTATATCAATATAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6854R-2.in silico     61  TTTTGAAATCCTGTGGTCTGGATGTAACCTCGATCAAGATTGACCCCTATATCAATATAG 120


6854R-2.IR_full       121 ATGCTGGAACCTTTTCGCCTTATGAGCATGGCGAG<span class="snp"><tt>N</tt></span>GTTTACGTTTTGGACGATGGCGCC 180
                          |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
6854R-2.in silico     121 ATGCTGGAACCTTTTCGCCTTATGAGCATGGCGAG<span class="snp"><tt>-</tt></span>GTTTACGTTTTGGACGATGGCGCC 180


6854R-2.IR_full       181 GAGGTGGATCTGGATCTGGGAAACTATGAACGGTTTTTGGATGTTACCCTGCATCGGGAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6854R-2.in silico     181 GAGGTGGATCTGGATCTGGGAAACTATGAACGGTTTTTGGATGTTACCCTGCATCGGGAC 240


6854R-2.IR_full       241 AACAACATAACCACCGGAAAAATTTACAAGTTGGTCATTGAGAAGGAGCGCACTGGCGAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6854R-2.in silico     241 AACAACATAACCACCGGAAAAATTTACAAGTTGGTCATTGAGAAGGAGCGCACTGGCGAG 300


6854R-2.IR_full       301 TACTTGGGCAAAACGGTTCAAGTTGTCCCACACATCACTGATGCCATTCAGGAATGGGTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6854R-2.in silico     301 TACTTGGGCAAAACGGTTCAAGTTGTCCCACACATCACTGATGCCATTCAGGAATGGGTG 360


6854R-2.IR_full       361 GA<span class="snp"><tt>N</tt></span>GCGCGTGGCCCAGACACCCGTTCAGGGATCTTCAAAGCCACAGGTGTGCATCGTGGA 420
                          ||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6854R-2.in silico     361 GA<span class="snp"><tt>-</tt></span>GCGCGTGGCCCAGACACCCGTTCAGGGATCTTCAAAGCCACAGGTGTGCATCGTGGA 420


6854R-2.IR_full       421 ATTGGGAGGAACGATTGGTGACATCGAAGGCATGCCTTTCGTAGAGGCCTTCCGTCAGTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6854R-2.in silico     421 ATTGGGAGGAACGATTGGTGACATCGAAGGCATGCCTTTCGTAGAGGCCTTCCGTCAGTT 480


6854R-2.IR_full       481 TCAGTTCCGCGTAAAGAGAGAG 502
                          ||||||||||||||||||||||
6854R-2.in silico     481 TCAGTTCCGCGTAAAGAGAGAG 502

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168606.1  CG6854-RC, transcript variant C (CG6854), mRNA 
68.87   332  1  1  3  NM_140490.2  CG6854-RB, transcript variant B (CG6854), mRNA 
0.2   1  2  2  2  NM_143320.2  CG18437-RA (CG18437), mRNA 
0   0  4  4  4  NM_176270.1  CG2469-RA, transcript variant A (CG2469), mRNA 
0   0  4  4  4  NM_176271.1  CG2469-RB, transcript variant B (CG2469), mRNA 
0   0  2  2  3  NM_057594.3  CG5848-RB, transcript variant B (cact), mRNA 
0   0  2  2  3  NM_205999.1  CG5848-RD, transcript variant D (cact), mRNA 
0   0  2  2  3  NM_165152.1  CG5848-RA, transcript variant A (cact), mRNA 
0   0  2  2  2  NM_057595.3  CG5848-RC, transcript variant C (cact), mRNA 
0   0  1  6  3  NM_170430.2  CG31037-RA (ca), mRNA 
0   0  1  5  2  NM_136675.1  CG1625-RA (CG1625), mRNA 
0   0  1  4  3  NM_136557.2  CG8635-RA (CG8635), mRNA 
0   0  1  3  6  NM_141501.1  CG3223-RA (CG3223), mRNA 
0   0  1  3  3  NM_140830.1  CG14080-RB, transcript variant B (Mkp3), mRNA 
0   0  1  2  2  NM_168648.1  CG32156-RB, transcript variant B (Mbs), mRNA 
0   0  1  2  2  NM_168647.1  CG32156-RA, transcript variant A (Mbs), mRNA 
0   0  1  2  2  NM_168646.1  CG32156-RC, transcript variant C (Mbs), mRNA 
0   0  1  2  2  NM_136736.1  CG12900-RA (CG12900), mRNA 
0   0  0  5  4  NM_169014.1  CG31531-RA, transcript variant A (CG31531), mRNA 
0   0  0  5  4  NM_169015.1  CG31531-RB, transcript variant B (CG31531), mRNA 
0   0  0  5  4  NM_176399.1  CG31531-RC, transcript variant C (CG31531), mRNA 
0   0  0  5  2  NM_132380.1  CG15311-RA (CG15311), mRNA 
0   0  0  4  7  NM_001043082.1  CG11798-RC, transcript variant C (chn), mRNA 
0   0  0  4  5  NM_001014669.1  CG5462-RI, transcript variant I (scrib), mRNA 
0   0  0  4  5  NM_142300.1  CG14888-RA (CG14888), mRNA 
0   0  0  4  4  NM_136867.1  CG8981-RB, transcript variant B (MCPH1), mRNA 
0   0  0  4  4  NM_165852.1  CG8981-RA, transcript variant A (MCPH1), mRNA 
0   0  0  3  14  NM_169790.1  CG7913-RA, transcript variant A (PP2A-B'), mRNA 
0   0  0  3  14  NM_169789.1  CG7913-RB, transcript variant B (PP2A-B'), mRNA 
0   0  0  3  6  NM_142637.1  CG10877-RA (CG10877), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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