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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6818R-2 
 Symbol MESR6  Full Name Misexpression suppressor of ras 6 
 CG No CG6818  Old CG No CG6818 
 Synonyms CG6818, MESR6 
 Accession No (Link to NCBI) NM_079430.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGAATCGGATACGCTGGAC 
 Primer Seq. 3' 
aatctagaggtaccGTGGGCATGATCCAGTTCTCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGGAATCGG ATACGCTGGA CCAGGAGAGT GCCGCGGCTG CTCTAAAAAA ACTATCGCCA 
0061 AGTGAAAGTC CCGAAAGTTC GAAGAAATGG GAGCCGACTT CGGGTGAGTG GGTTGCGGCG 
0121 GGTGGGGGAG AAGAGGCGCC GCCAACAAAG AGACAAAAGA CTGCAGACGA AAAGGAGGAG 
0181 AATCGGGAGG AAGACGAGGA TAGCGAGGAG AGCGTTGATG AGGAGGAGGA TGATGGCGAG 
0241 GGCATCGTCC AGATTGGCGG CACCATCAGC CCAAGGCATT TCCAGCGCAT CCCCGTCTTT 
0301 ATCGTCGACT ACCACAACGA TGTGCTGGAG TTCATCTACC GCTGCTTTGC CACCCGCCAC 
0361 CTTCCGCTGC AGAACAACAC CCTTGTCCAC TTCGACTCGC ATCCGGATCT GGTCATTCCA 
0421 CGGCACATTG CCGCCAGTTC CACCTACCAA AAGGAAACCA TGTTAAATGA GCTTAGCATC 
0481 GAGAACTGGA TCATGCCCAC  
 in silico PCR Fragment 
0001 ATGGAATCGG ATACGCTGGA CCAGGAGAGT GCCGCGGCTG CTCTAAAAAA ACTATCGCCA 
0061 AGTGAAAGTC CCGAAAGTTC GAAGAAATGG GAGCCGACTT CGGGAGAGTG GGTTGCGGCG 
0121 GGTGGGGGAG AAGAGGCGCC GCCAACAAAG AGACAAAAGA CTGCAGACGA AAAGGAGGAG 
0181 AATCGGGAGG AAGACGAGGA TAGCGAGGAG AGCGTTGATG AGGAGGAGGA TGATGGCGAG 
0241 GGCATCGTCC AGATTGGCGG CACCATCAGC CCAAGGCATT TCCAGCGCAT CCCCGTCTTT 
0301 ATCGTCGACT ACCACAACGA TGTGCTGGAG TTCATCTACC GCTGCTTTGC CACCCGCCAC 
0361 CTTCCGCTGC AGAACAACAC CCTTGTCCAC TTCGACTCGC ATCCGGATCT GGTCATTCCA 
0421 CGGCACATTG CCGCCAGTTC CACCTACCAA AAGGAAACCA TGTTAAATGA GCTTAGCATC 
0481 GAGAACTGGA TCATGCCCAC  
 Assemble Data 
<PRE>

6818R-2.IR_full       1   ATGGAATCGGATACGCTGGACCAGGAGAGTGCCGCGGCTGCTCTAAAAAAACTATCGCCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6818R-2.in silico     1   ATGGAATCGGATACGCTGGACCAGGAGAGTGCCGCGGCTGCTCTAAAAAAACTATCGCCA 60


6818R-2.IR_full       61  AGTGAAAGTCCCGAAAGTTCGAAGAAATGGGAGCCGACTTCGGG<span class="snp"><tt>T</tt></span>GAGTGGGTTGCGGCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||
6818R-2.in silico     61  AGTGAAAGTCCCGAAAGTTCGAAGAAATGGGAGCCGACTTCGGG<span class="snp"><tt>A</tt></span>GAGTGGGTTGCGGCG 120


6818R-2.IR_full       121 GGTGGGGGAGAAGAGGCGCCGCCAACAAAGAGACAAAAGACTGCAGACGAAAAGGAGGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6818R-2.in silico     121 GGTGGGGGAGAAGAGGCGCCGCCAACAAAGAGACAAAAGACTGCAGACGAAAAGGAGGAG 180


6818R-2.IR_full       181 AATCGGGAGGAAGACGAGGATAGCGAGGAGAGCGTTGATGAGGAGGAGGATGATGGCGAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6818R-2.in silico     181 AATCGGGAGGAAGACGAGGATAGCGAGGAGAGCGTTGATGAGGAGGAGGATGATGGCGAG 240


6818R-2.IR_full       241 GGCATCGTCCAGATTGGCGGCACCATCAGCCCAAGGCATTTCCAGCGCATCCCCGTCTTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6818R-2.in silico     241 GGCATCGTCCAGATTGGCGGCACCATCAGCCCAAGGCATTTCCAGCGCATCCCCGTCTTT 300


6818R-2.IR_full       301 ATCGTCGACTACCACAACGATGTGCTGGAGTTCATCTACCGCTGCTTTGCCACCCGCCAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6818R-2.in silico     301 ATCGTCGACTACCACAACGATGTGCTGGAGTTCATCTACCGCTGCTTTGCCACCCGCCAC 360


6818R-2.IR_full       361 CTTCCGCTGCAGAACAACACCCTTGTCCACTTCGACTCGCATCCGGATCTGGTCATTCCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6818R-2.in silico     361 CTTCCGCTGCAGAACAACACCCTTGTCCACTTCGACTCGCATCCGGATCTGGTCATTCCA 420


6818R-2.IR_full       421 CGGCACATTGCCGCCAGTTCCACCTACCAAAAGGAAACCATGTTAAATGAGCTTAGCATC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6818R-2.in silico     421 CGGCACATTGCCGCCAGTTCCACCTACCAAAAGGAAACCATGTTAAATGAGCTTAGCATC 480


6818R-2.IR_full       481 GAGAACTGGATCATGCCCAC 500
                          ||||||||||||||||||||
6818R-2.in silico     481 GAGAACTGGATCATGCCCAC 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_079430.2  CG6818-RA (MESR6), mRNA 
0.41   2  4  5  10  NM_135222.2  CG11188-RA (CG11188), mRNA 
0.2   1  7  3  15  NM_140532.2  CG7372-RA (CG7372), mRNA 
0.2   1  2  7  19  NM_132280.3  CG10648-RA (CG10648), mRNA 
0   0  5  7  14  NM_143344.2  CG5520-RA (Gp93), mRNA 
0   0  5  3  2  NM_170510.1  CG2246-RA, transcript variant A (CG2246), mRNA 
0   0  5  3  2  NM_170511.1  CG2246-RC, transcript variant C (CG2246), mRNA 
0   0  5  3  2  NM_170507.1  CG2246-RB, transcript variant B (CG2246), mRNA 
0   0  5  3  2  NM_170508.1  CG2246-RD, transcript variant D (CG2246), mRNA 
0   0  5  3  2  NM_170509.1  CG2246-RF, transcript variant F (CG2246), mRNA 
0   0  5  3  2  NM_143556.2  CG2246-RE, transcript variant E (CG2246), mRNA 
0   0  4  6  16  NM_130585.2  CG14810-RA (CG14810), mRNA 
0   0  3  8  14  NM_176746.1  CG5424-RC, transcript variant C (f), mRNA 
0   0  3  6  3  NM_057362.2  CG17579-RA, transcript variant A (sca), mRNA 
0   0  3  6  3  NM_165951.1  CG17579-RB, transcript variant B (sca), mRNA 
0   0  3  4  11  NM_139950.1  CG13670-RA (CG13670), mRNA 
0   0  3  4  5  NM_131954.1  CG7024-RA (CG7024), mRNA 
0   0  3  2  3  NM_132313.1  CG15368-RA (CG15368), mRNA 
0   0  2  11  35  NM_079967.4  CG6611-RA, transcript variant A (ect), mRNA 
0   0  2  11  35  NM_168400.1  CG6611-RB, transcript variant B (ect), mRNA 
0   0  2  11  34  NM_168401.1  CG6611-RC, transcript variant C (ect), mRNA 
0   0  2  7  35  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
0   0  2  7  35  NM_176556.1  CG33106-RA, transcript variant A (mask), mRNA 
0   0  2  7  8  NM_079569.3  CG9429-RA (Crc), mRNA 
0   0  2  6  11  NM_131944.1  CG3546-RA (CG3546), mRNA 
0   0  2  5  17  NM_139541.1  CG12017-RA (CG12017), mRNA 
0   0  2  5  7  NM_079537.2  CG1112-RA, transcript variant A (alpha-Est7), mRNA 
0   0  2  3  19  NM_168367.1  CG32046-RA, transcript variant A (CG32046), mRNA 
0   0  2  3  19  NM_168366.2  CG32046-RB, transcript variant B (CG32046), mRNA 
0   0  2  3  14  NM_078582.3  CG32659-RA (Ten-a), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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