|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6806R-2 |
| Symbol |
Lsp2 |
Full Name |
Larval serum protein 2 |
| CG No |
CG6806 |
Old CG No |
CG6806 |
| Synonyms |
Lsp-2, CG6806, C23, DmeLSP2, LSP-2, LSP 2, Pt-1, LSP2, LHP, Lsp2 |
| Accession No (Link to NCBI) |
NM_080077.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Shibata T, Ariki S, Shinzawa N, Miyaji R, Suyama H, Sako M, Inomata N, Koshiba T, Kanuka H, Kawabata S.<br> Protein crosslinking by transglutaminase controls cuticle morphogenesis in Drosophila.<br> PLoS One (2010) 5(10) e13477
[
PubMed ID = 20976106
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAGTCGTTCACGGTGATTGC |
| Primer Seq. 3' |
aatctagaggtaccATGGGTGGATCTCGTAGTGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NAAGTCGTTC ACGNTGATTG CGNTNGcTNC AGTGGCATTG CTGGCTACTC TCGGCCAGGC
0061 CAAGCACCTG GACTCCAAGG TGGCGGACAA GGACTTTCTG ATGAAACAGA AGTTCATGTA
0121 CCAGATCCTG CAGCACATCT ACCAGGACGA TGTGTTCACC ACNCCCTTCG GGGGCAGCTN
0181 ACGTGGAGTA CAAGCCGTGG GAGCACGTGG CCGACTATGT GCACCCGGAG ATGTTGGAGC
0241 ACTTCTTCGA GCTGTGGCAA CACCAACCCT TCACCGATGA CATGGTGTGG TCGGTGATGT
0301 ACGACAAGCA CGAGGAGTAT GTGGTGGGTC TGGTGCGTCT CTTCTACTTC GCCAAGAACT
0361 GGGAGACCTT CCAGCACGTC GTCTACTGGG CCCGCCAGCA CGTAAACAAG CAGTTGTTCG
0421 TGTACGCCGT GACCATTGCC AGTTTGTTCC GTGATGATAT GCAGGGAGTC NNCCTGCCGG
0481 CCCACTACGA GATCCACCCA T |
| in silico PCR Fragment |
0001 GAAGTCGTTC ACGGTGATTG CGTTAGCTGC AGTGGCATTG CTGGCTACTC TCGGCCAGGC
0061 CAAGCACCTG GACTCCAAGG TGGCGGACAA GGACTTTCTG ATGAAACAGA AGTTCATGTA
0121 CCAGATCCTG CAGCACATCT ACCAGGACGA TGTGTTCACC ACGCCCTTCG GGGGCAGCT-
0181 ACGTGGAGTA CAAGCCGTGG GAGCACGTGG CCGACTATGT GCACCCGGAG ATGTTGGAGC
0241 ACTTCTTCGA GCTGTGGCAA CACCAACCCT TCACCGATGA CATGGTGTGG TCGGTGATGT
0301 ACGACAAGCA CGAGGAGTAT GTGGTGGGTC TGGTGCGTCT CTTCTACTTC GCCAAGAACT
0361 GGGAGACCTT CCAGCACGTC GTCTACTGGG CCCGCCAGCA CGTAAACAAG CAGTTGTTCG
0421 TGTACGCCGT GACCATTGCC AGTTTGTTCC GTGATGATAT GCAGGGAGTC GTCCTGCCGG
0481 CCCACTACGA GATCCACCCA T |
| Assemble Data |
<PRE>
6806R-2.IR_full 1 AAGTCGTTCACG<span class="snp"><tt>N</tt></span>TGATTGCG<span class="snp"><tt>N</tt></span>T<span class="snp"><tt>N</tt></span>GCT<span class="snp"><tt>N</tt></span>CAGTGGCATTGCTGGCTACTCTCGGCCAGGC 59
||||||||||||<span class="snp"><tt> </tt></span>||||||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>|||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||
6806R-2.in silico 1 AAGTCGTTCACG<span class="snp"><tt>G</tt></span>TGATTGCG<span class="snp"><tt>T</tt></span>T<span class="snp"><tt>A</tt></span>GCT<span class="snp"><tt>G</tt></span>CAGTGGCATTGCTGGCTACTCTCGGCCAGGC 59
6806R-2.IR_full 61 CAAGCACCTGGACTCCAAGGTGGCGGACAAGGACTTTCTGATGAAACAGAAGTTCATGTA 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6806R-2.in silico 61 CAAGCACCTGGACTCCAAGGTGGCGGACAAGGACTTTCTGATGAAACAGAAGTTCATGTA 119
6806R-2.IR_full 121 CCAGATCCTGCAGCACATCTACCAGGACGATGTGTTCACCAC<span class="snp"><tt>N</tt></span>CCCTTCGGGGGCAGCT<span class="snp"><tt>N</tt></span> 179
||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||<span class="snp"><tt> </tt></span>
6806R-2.in silico 121 CCAGATCCTGCAGCACATCTACCAGGACGATGTGTTCACCAC<span class="snp"><tt>G</tt></span>CCCTTCGGGGGCAGCT<span class="snp"><tt>-</tt></span> 179
6806R-2.IR_full 181 ACGTGGAGTACAAGCCGTGGGAGCACGTGGCCGACTATGTGCACCCGGAGATGTTGGAGC 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6806R-2.in silico 181 ACGTGGAGTACAAGCCGTGGGAGCACGTGGCCGACTATGTGCACCCGGAGATGTTGGAGC 239
6806R-2.IR_full 241 ACTTCTTCGAGCTGTGGCAACACCAACCCTTCACCGATGACATGGTGTGGTCGGTGATGT 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6806R-2.in silico 241 ACTTCTTCGAGCTGTGGCAACACCAACCCTTCACCGATGACATGGTGTGGTCGGTGATGT 299
6806R-2.IR_full 301 ACGACAAGCACGAGGAGTATGTGGTGGGTCTGGTGCGTCTCTTCTACTTCGCCAAGAACT 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6806R-2.in silico 301 ACGACAAGCACGAGGAGTATGTGGTGGGTCTGGTGCGTCTCTTCTACTTCGCCAAGAACT 359
6806R-2.IR_full 361 GGGAGACCTTCCAGCACGTCGTCTACTGGGCCCGCCAGCACGTAAACAAGCAGTTGTTCG 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6806R-2.in silico 361 GGGAGACCTTCCAGCACGTCGTCTACTGGGCCCGCCAGCACGTAAACAAGCAGTTGTTCG 419
6806R-2.IR_full 421 TGTACGCCGTGACCATTGCCAGTTTGTTCCGTGATGATATGCAGGGAGTC<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>CCTGCCGG 479
||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||
6806R-2.in silico 421 TGTACGCCGTGACCATTGCCAGTTTGTTCCGTGATGATATGCAGGGAGTC<span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span>CCTGCCGG 479
6806R-2.IR_full 481 CCCACTACGAGATCCACCCAT 500
|||||||||||||||||||||
6806R-2.in silico 481 CCCACTACGAGATCCACCCAT 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
10 |
NM_080077.2 |
CG6806-RA (Lsp2), mRNA |
| 97.51 |
470 |
0 |
1 |
10 |
NM_143790.1 |
CG11538-RA (CG11538), mRNA |
| 0 |
0 |
4 |
23 |
24 |
NM_057276.3 |
CG4178-RA (Lsp1beta), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_166585.2 |
CG30189-RA (Gr59a), mRNA |
| 0 |
0 |
3 |
6 |
2 |
NM_133090.1 |
CG6696-RA (CG6696), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_141559.3 |
CG8112-RA (CG8112), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_165618.1 |
CG8579-RA (Jon44E), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_138268.1 |
CG12084-RA (CG12084), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167145.1 |
CG2257-RA, transcript variant A (Ubc-E2H), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167146.1 |
CG2257-RC, transcript variant C (Ubc-E2H), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_132210.2 |
CG2257-RB, transcript variant B (Ubc-E2H), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_168252.1 |
CG7892-RB, transcript variant B (nmo), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_168249.1 |
CG7892-RF, transcript variant F (nmo), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_168251.1 |
CG7892-RA, transcript variant A (nmo), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_168248.1 |
CG7892-RE, transcript variant E (nmo), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_079243.2 |
CG7892-RC, transcript variant C (nmo), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_168250.1 |
CG7892-RG, transcript variant G (nmo), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_132040.1 |
CG15766-RA (CG15766), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_134964.1 |
CG3921-RA (CG3921), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_137739.2 |
CG10795-RA (CG10795), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_144171.1 |
CG13479-RA (CG13479), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141130.3 |
CG11489-RB, transcript variant B (CG11489), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001014681.1 |
CG33553-RD, transcript variant D (Doa), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_133020.2 |
CG6506-RA (CG6506), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141576.1 |
CG11775-RA (CG11775), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001043298.1 |
CG33553-RH, transcript variant H (Doa), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137701.2 |
CG4030-RA (CG4030), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001038936.2 |
CG33989-RE, transcript variant E (pHCl), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001038934.2 |
CG33989-RD, transcript variant D (pHCl), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143222.1 |
CG14237-RA (CG14237), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
