NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6778R-1

Symbol

Aats-gly

Full Name

Glycyl-tRNA synthetase

CG No

CG6778

Old CG No

CG6778

Synonyms

CG6778, GlyRS, GRS, Aats-gly, gars

Accession No (Link to NCBI)

NM_140489.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTGTTAAAAGCGCTACCCCAC

Primer Seq. 3'

aatctagaggtaccGCGAAACTCTGGTCGTAGAAG

Predicted Fragment Size

IR fragment full Seq

0001 TTGTTAAAAG CGCTACCCCA CCTGCGCAGC GcCACCACAG CCGTGAGAAC TCAGATTGCA 
0061 AGAACAACGT GGGAGCGAGC ACATTGCCAC GAAAGtGTTT TTTAGCACCA CTACCACAAA 
0121 ACCTACTCCC TCCGCACCAC CACCACCTCC ACCAACGCAA CCGCAGCAGC CAGTTCCACC 
0181 TGCAACCACA AGTTGGGGCA CCAAAAAGCA AAATAGAAAG GTAAAACTAA GATCCGCCGC 
0241 TGCCGAGTTC ATCATGTCGA ATCCCGAGAT TGAGGCTCAA CTTGCCCCAT TGAGGGAACG 
0301 GAGTTCAGGA GCAGGGTAAC CTGGTGAGGG ATCTCAAGGC GAAGGGTGCC CCCGAGATCG 
0361 ATGTGAAAAA GGCAGTAGCC GAGCTGAAGG CACGCAAGAA GCTGCTGGAG GACAAAGAGC 
0421 TCGCCCTGAC CCCCAGTGTC GTTAGTTTCG ATCGCGCGAA GATGGAGGAT CTACTCAAGc 
0481 GTCGCTTCTT CTACGACCAG AGTTTCGC

in silico PCR Fragment

0001 TTGTTAAAAG CGCTACCCCA CCTGCGCAGC GCCACCACAG CCGTGAGAAC TCAGATTGCA 
0061 AGAACAACGT -GGAGCGAGC ACATTGCCAC GAAAGTGTTT TTTAGCACCA CTACCACAAA 
0121 ACCTACTCCC TCCGCACCAC CACCACCTCC ACCAACGCAA CCGCAGCAGC CAG------C 
0181 TGCAACCACA AGTTGGGGCA CCAAGAAGCA AAATAGAAAG GTAAAACTAA GATCCGCCGC 
0241 TGCCGAGTTC ATCATGTCGA ATCCCGAGAT TGAGGCTCAA CTTGCCCCAT TGAGGGAAC- 
0301 GAGTTCAGGA GCAGGGTAAC CTGGTGAGGG ATCTCAAGGC GAAGGGTGCC CCCGAGATCG 
0361 ATGTGAAAAA GGCAGTAGCC GAGCTGAAGG CACGCAAGAA GCTGCTGGAG GACAAAGAGC 
0421 TCGCCCTGAC CCCCAGTGTC GTTAGTTTCG ATCGCGCGAA GATGGAGGAT CTACTCAAGC 
0481 GTCGCTTCTT CTACGACCAG AGTTTCGC

Assemble Data

<PRE>

6778R-1.IR_full       1   TTGTTAAAAGCGCTACCCCACCTGCGCAGCGCCACCACAGCCGTGAGAACTCAGATTGCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6778R-1.in silico     1   TTGTTAAAAGCGCTACCCCACCTGCGCAGCGCCACCACAGCCGTGAGAACTCAGATTGCA 60


6778R-1.IR_full       61  AGAACAACGT<span class="snp"><tt>G</tt></span>GGAGCGAGCACATTGCCACGAAAGTGTTTTTTAGCACCACTACCACAAA 120
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
6778R-1.in silico     61  AGAACAACGT<span class="snp"><tt>-</tt></span>GGAGCGAGCACATTGCCACGAAAGTGTTTTTTAGCACCACTACCACAAA 120


6778R-1.IR_full       121 ACCTACTCCCTCCGCACCACCACCACCTCCACCAACGCAACCGCAGCAGCCAG<span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span>C 180
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|
6778R-1.in silico     121 ACCTACTCCCTCCGCACCACCACCACCTCCACCAACGCAACCGCAGCAGCCAG<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>C 180


6778R-1.IR_full       181 TGCAACCACAAGTTGGGGCACCAA<span class="snp"><tt>A</tt></span>AAGCAAAATAGAAAGGTAAAACTAAGATCCGCCGC 240
                          ||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
6778R-1.in silico     181 TGCAACCACAAGTTGGGGCACCAA<span class="snp"><tt>G</tt></span>AAGCAAAATAGAAAGGTAAAACTAAGATCCGCCGC 240


6778R-1.IR_full       241 TGCCGAGTTCATCATGTCGAATCCCGAGATTGAGGCTCAACTTGCCCCATTGAGGGAAC<span class="snp"><tt>G</tt></span> 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
6778R-1.in silico     241 TGCCGAGTTCATCATGTCGAATCCCGAGATTGAGGCTCAACTTGCCCCATTGAGGGAAC<span class="snp"><tt>-</tt></span> 300


6778R-1.IR_full       301 GAGTTCAGGAGCAGGGTAACCTGGTGAGGGATCTCAAGGCGAAGGGTGCCCCCGAGATCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6778R-1.in silico     301 GAGTTCAGGAGCAGGGTAACCTGGTGAGGGATCTCAAGGCGAAGGGTGCCCCCGAGATCG 360


6778R-1.IR_full       361 ATGTGAAAAAGGCAGTAGCCGAGCTGAAGGCACGCAAGAAGCTGCTGGAGGACAAAGAGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6778R-1.in silico     361 ATGTGAAAAAGGCAGTAGCCGAGCTGAAGGCACGCAAGAAGCTGCTGGAGGACAAAGAGC 420


6778R-1.IR_full       421 TCGCCCTGACCCCCAGTGTCGTTAGTTTCGATCGCGCGAAGATGGAGGATCTACTCAAGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6778R-1.in silico     421 TCGCCCTGACCCCCAGTGTCGTTAGTTTCGATCGCGCGAAGATGGAGGATCTACTCAAGC 480


6778R-1.IR_full       481 GTCGCTTCTTCTACGACCAGAGTTTCGC 508
                          ||||||||||||||||||||||||||||
6778R-1.in silico     481 GTCGCTTCTTCTACGACCAGAGTTTCGC 508

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_140489.2	CG6778-RA, transcript variant A (Aats-gly), mRNA
100	482	0	0	2	NM_168605.1	CG6778-RB, transcript variant B (Aats-gly), mRNA
0.41	2	6	25	44	NM_132661.1	CG12726-RA (CG12726), mRNA
0	0	10	18	27	NM_132391.2	CG15307-RA (CG15307), mRNA
0	0	10	16	28	NM_169047.1	CG32464-RD, transcript variant D (l(3)82Fd), mRNA
0	0	9	21	34	NM_132214.1	CG15335-RB (CG15335), mRNA
0	0	7	21	39	NM_176328.1	CG17364-RB, transcript variant B (CG17364), mRNA
0	0	7	21	39	NM_140417.2	CG17364-RA, transcript variant A (CG17364), mRNA
0	0	7	18	37	NM_134552.3	CG1412-RA (RhoGAP19D), mRNA
0	0	7	18	35	NM_001038911.1	CG34050-RA (CG34050), mRNA
0	0	6	16	34	NM_169142.1	CG10272-RB, transcript variant B (gpp), mRNA
0	0	6	16	34	NM_141398.1	CG10272-RA, transcript variant A (gpp), mRNA
0	0	6	16	34	NM_169143.1	CG10272-RD, transcript variant D (gpp), mRNA
0	0	6	16	34	NM_169144.1	CG10272-RC, transcript variant C (gpp), mRNA
0	0	6	14	24	NM_206663.1	CG12075-RB, transcript variant B (CG12075), mRNA
0	0	6	14	24	NM_132278.2	CG12075-RA, transcript variant A (CG12075), mRNA
0	0	6	13	29	NM_138223.1	CG12502-RA (CG12502), mRNA
0	0	6	11	20	NM_143633.1	CG2187-RA (CG2187), mRNA
0	0	6	7	19	NM_131997.1	CG16752-RA (CG16752), mRNA
0	0	6	7	3	NM_130715.2	CG13316-RB, transcript variant B (Mnt), mRNA
0	0	6	7	2	NM_166983.1	CG13316-RA, transcript variant A (Mnt), mRNA
0	0	6	7	2	NM_166984.1	CG13316-RC, transcript variant C (Mnt), mRNA
0	0	5	27	54	NM_142330.1	CG5225-RA (CG5225), mRNA
0	0	5	16	32	NM_135952.1	CG5953-RA, transcript variant A (CG5953), mRNA
0	0	5	16	32	NM_165165.1	CG5953-RB, transcript variant B (CG5953), mRNA
0	0	5	16	26	NM_132209.1	CG2256-RB (CG2256), mRNA
0	0	5	14	26	NM_132009.2	CG11473-RA (CG11473), mRNA
0	0	5	14	23	NM_130497.2	CG13363-RA (Suv4-20), mRNA
0	0	5	12	23	NM_001043110.1	CG6883-RB, transcript variant B (trh), mRNA
0	0	5	12	23	NM_079148.2	CG6883-RA, transcript variant A (trh), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00970 : Aminoacyl-tRNA biosynthesis

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