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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6735R-2

Symbol

cp309

Full Name

cp309

CG No

CG33957

Old CG No

CG6735

Synonyms

D-PLP, CG6735, CG18648, CG13459, d-plp, CP309, AKAP450, l(3)s2172, CG33957, cp309, PACT, dplp

Accession No (Link to NCBI)

NM_001032402.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCTTCAACATGAGCGGGATA

Primer Seq. 3'

aatctagaggtaccCTGTTGAGATGCCTGCAGTTC

Predicted Fragment Size

IR fragment full Seq

0001 AGCTTCAACA TGAGCGGGAT ACgCTGCAGG GTCGTATGGA GGAACAGACT CAGCGTATTT 
0061 CTACCCTCCA AAATCGGCTG GAGGAGCAGC GACAGCGGGC GGAGCAACTC CATCGGACTG 
0121 GCACATCAGA CTTGAATACC CGAGTACATG AACTCCAGGG TGAGGTCCAG AACCTATACG 
0181 AGCAATTGGC GGCGCGGGAC AAGCAGATGG CCAACATGCG ACAGCAANTG CAGCGCAGCA 
0241 AGGAGGAGAT AACTCGACTG GAGACGGAAG TCGAAGTACG AACACAACCA GATCGCAGTC 
0301 TGGTGAACAA ACTGCAGGCT GAAGTGCAGC AAAAGGGAGC AGAGATCGTG AAGTTAAAGG 
0361 ATAAGATACG CACAGAGATG ATCAACCGTC TGGCTATCCC GGATCTCATG GAGACTATGC 
0421 TGGCGGACAA GAACGATGAG ATAGATCACC TGCGCGATCA ACTGGAGGCT AAGGAGAAGG 
0481 AACTGCAGGC ATCTCAACAG

in silico PCR Fragment

0001 AGCTTCAACA TGAGCGGGAT ACGCTGCAGG GTCGTATGGA GGAACAGACT CAGCGTATTT 
0061 CTACCCTCCA AAATCGGCTG GAGGAGCAGC GACAGCGGGC GGAGCAACTC CATCGGACTG 
0121 GCACATCAGA CTTGAATACC CGAGTACATG AACTCCAGGG TGAGGTCCAG AACCTATACG 
0181 AGCAATTGGC GGCGCGGGAC AAGCAGATGG CCAACATGCG ACAGCAACTG CAGCGCAGCA 
0241 AGGAGGAGAT AACTCGACTG GAGACGGAAG TCGAAGTACG AACACAACCA GATCGCAGTC 
0301 TGGTGAACAA ACTGCAGGCT GAAGTGCAGC AAAAGGGAGC AGAGATCGTG AAGTTAAAGG 
0361 ATAAGATACG CACAGAGATG ATCAACCGTC TGGCTATCCC GGATCTCATG GAGACTATGC 
0421 TGGCGGACAA GAACGATGAG ATAGATCACC TGCGCGATCA ACTGGAGGCT AAGGAGAAGG 
0481 AACTGCAGGC ATCTCAACAG

Assemble Data

<PRE>

6735R-2.IR_full       1   AGCTTCAACATGAGCGGGATACGCTGCAGGGTCGTATGGAGGAACAGACTCAGCGTATTT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6735R-2.in silico     1   AGCTTCAACATGAGCGGGATACGCTGCAGGGTCGTATGGAGGAACAGACTCAGCGTATTT 60


6735R-2.IR_full       61  CTACCCTCCAAAATCGGCTGGAGGAGCAGCGACAGCGGGCGGAGCAACTCCATCGGACTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6735R-2.in silico     61  CTACCCTCCAAAATCGGCTGGAGGAGCAGCGACAGCGGGCGGAGCAACTCCATCGGACTG 120


6735R-2.IR_full       121 GCACATCAGACTTGAATACCCGAGTACATGAACTCCAGGGTGAGGTCCAGAACCTATACG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6735R-2.in silico     121 GCACATCAGACTTGAATACCCGAGTACATGAACTCCAGGGTGAGGTCCAGAACCTATACG 180


6735R-2.IR_full       181 AGCAATTGGCGGCGCGGGACAAGCAGATGGCCAACATGCGACAGCAA<span class="snp"><tt>N</tt></span>TGCAGCGCAGCA 240
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
6735R-2.in silico     181 AGCAATTGGCGGCGCGGGACAAGCAGATGGCCAACATGCGACAGCAA<span class="snp"><tt>C</tt></span>TGCAGCGCAGCA 240


6735R-2.IR_full       241 AGGAGGAGATAACTCGACTGGAGACGGAAGTCGAAGTACGAACACAACCAGATCGCAGTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6735R-2.in silico     241 AGGAGGAGATAACTCGACTGGAGACGGAAGTCGAAGTACGAACACAACCAGATCGCAGTC 300


6735R-2.IR_full       301 TGGTGAACAAACTGCAGGCTGAAGTGCAGCAAAAGGGAGCAGAGATCGTGAAGTTAAAGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6735R-2.in silico     301 TGGTGAACAAACTGCAGGCTGAAGTGCAGCAAAAGGGAGCAGAGATCGTGAAGTTAAAGG 360


6735R-2.IR_full       361 ATAAGATACGCACAGAGATGATCAACCGTCTGGCTATCCCGGATCTCATGGAGACTATGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6735R-2.in silico     361 ATAAGATACGCACAGAGATGATCAACCGTCTGGCTATCCCGGATCTCATGGAGACTATGC 420


6735R-2.IR_full       421 TGGCGGACAAGAACGATGAGATAGATCACCTGCGCGATCAACTGGAGGCTAAGGAGAAGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6735R-2.in silico     421 TGGCGGACAAGAACGATGAGATAGATCACCTGCGCGATCAACTGGAGGCTAAGGAGAAGG 480


6735R-2.IR_full       481 AACTGCAGGCATCTCAACAG 500
                          ||||||||||||||||||||
6735R-2.in silico     481 AACTGCAGGCATCTCAACAG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_001032402.1	CG33957-RB, transcript variant B (cp309), mRNA
100	482	0	0	0	NM_001032401.1	CG33957-RC, transcript variant C (cp309), mRNA
0	0	5	6	3	NM_142439.1	CG7998-RA (CG7998), mRNA
0	0	4	3	12	NM_057326.4	CG12212-RA, transcript variant A (peb), mRNA
0	0	4	3	12	NM_001014722.1	CG12212-RB, transcript variant B (peb), mRNA
0	0	3	5	2	NM_136879.2	CG8364-RA, transcript variant A (Rep3), mRNA
0	0	3	5	2	NM_136878.2	CG8364-RB, transcript variant B (Rep3), mRNA
0	0	3	3	5	NM_169609.1	CG31304-RA (CG31304), mRNA
0	0	3	2	4	NM_134517.2	CG15618-RA (CG15618), mRNA
0	0	2	7	8	NM_140284.1	CG6793-RA (CG6793), mRNA
0	0	2	5	4	NM_166958.1	CG3653-RA, transcript variant A (kirre), mRNA
0	0	2	5	3	NM_080320.2	CG3653-RB, transcript variant B (kirre), mRNA
0	0	2	4	6	NM_132622.1	CG18646-RA (CG18646), mRNA
0	0	2	4	2	NM_137544.2	CG15104-RA (Topors), mRNA
0	0	2	2	4	NM_137992.1	CG5549-RA (CG5549), mRNA
0	0	2	2	3	NM_135034.1	CG15624-RA, transcript variant A (hoe2), mRNA
0	0	2	2	2	NM_078529.3	CG18009-RD, transcript variant D (Trf2), mRNA
0	0	2	2	2	NM_206654.1	CG18009-RA, transcript variant A (Trf2), mRNA
0	0	2	2	2	NM_141134.1	CG11440-RA (CG11440), mRNA
0	0	1	6	11	NM_137212.2	CG30089-RA (CG30089), mRNA
0	0	1	4	12	NM_134516.2	CG32529-RA, transcript variant A (CG32529), mRNA
0	0	1	4	11	NM_133124.1	CG7502-RA (CG7502), mRNA
0	0	1	4	9	NM_001032399.1	CG33955-RB (eys), mRNA
0	0	1	4	6	NM_167387.1	CG32611-RB (CG32611), mRNA
0	0	1	4	3	NM_136522.1	CG14760-RA (CG14760), mRNA
0	0	1	4	2	NM_142727.1	CG6800-RA (CG6800), mRNA
0	0	1	3	17	NM_078645.2	CG4252-RA (mei-41), mRNA
0	0	1	3	4	NM_137130.2	CG10131-RA (CG10131), mRNA
0	0	1	3	2	NM_141332.2	CG1218-RA (CG1218), mRNA
0	0	1	2	13	NM_164966.2	CG6464-RA (salm), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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