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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6322R-2 
 Symbol CG6322  Full Name CG6322 
 CG No CG6322  Old CG No CG6322 
 Synonyms CG6322 
 Accession No (Link to NCBI) NM_140733.2 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTACCACGAGGGACCCGATAGT 
 Primer Seq. 3' 
aatctagaggtaccGGATGGAAGGCTACCTTAGAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TACCACGAGG GACCCGATAG TTTGCGTATC GCTCGACTCT GGCTGGCGGA TTATTNCCCT 
0061 GCCGCGCGCC AAGGATCGCT TGGTACGAGC ACGTGAGGCC CTTGAAGTGC CCANGCGCAN 
0121 GCACGTGCCG GCCGAATGGT GGAGATGCAG AAGAAGCTCC AGTCGCTGGC TCCACTGTGC 
0181 TCCCAGGTGG GATGACACAC GGCCCGTGAG TAGTGCTGCC TTCAGTGAAG ACTCCTCACT 
0241 CCTTCTGACC TCCTCATGGT CGGGTCTCTG CAAGCTGTGG AGCGTGCCCG ATTGCGAGCT 
0301 GAAGCAGACG CTGCGTGGAC ATGCCAGCTA TGTGGGCGGA NGTGGCTCTG CGGCCGGGAG 
0361 TTAAGGCAGA CGAGGAGAAC GTGGTGGCCA TGGCTTNCCG GTGGCCACGA TGGCGCCGTC 
0421 AAGCTGTGGG GCTTCAACAA CGAAGAGTCG ATTGCCGACA TCACCGGCCA CATGCCGCAT 
0481 CGCGTCNTCT AAGGTAGCCT TCCATCC 
 in silico PCR Fragment 
0001 TACCACGAGG GACCCGATAG TTTGCGTATC GCTCGACTCT GGCTGGCGGA TTATT-CCCT 
0061 GCCGCGCGCC AAGGATCGCT TGGTACGAGC ACGTGAGGCC CTTGAAGTGC CCA-GCGCA- 
0121 GCACGTGCCG GCCGAATGGT GGAGATGCAG AAGAAGCTCC AGTCGCTGGC TCCACTGTGC 
0181 TCCCAGGTGG G-TGACACAC GGCCCGTGAG TAGTGCTGCC TTCAGTGAAG ACTCCTCACT 
0241 CCTTCTGACC TCCTCATGGT CGGGTCTCTG CAAGCTGTGG AGCGTGCCCG ATTGCGAGCT 
0301 GAAGCAGACG CTGCGTGGAC ATGCCAGCTA TGTGGGCGGA -GTGGCTCTG CGGCCGGGAG 
0361 TTAAGGCAGA CGAGGAGAAC GTGGTGGCCA TGGCTT-CCG GTGGCCACGA TGGCGCCGTC 
0421 AAGCTGTGGG GCTTCAACAA CGAAGAGTCG ATTGCCGACA TCACCGGCCA CATGCCGCAT 
0481 CGCGTC-TCT AAGGTAGCCT TCCATCC 
 Assemble Data 
<PRE>

6322R-2.IR_full       1   TACCACGAGGGACCCGATAGTTTGCGTATCGCTCGACTCTGGCTGGCGGATTATT<span class="snp"><tt>N</tt></span>CCCT 60
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
6322R-2.in silico     1   TACCACGAGGGACCCGATAGTTTGCGTATCGCTCGACTCTGGCTGGCGGATTATT<span class="snp"><tt>-</tt></span>CCCT 60


6322R-2.IR_full       61  GCCGCGCGCCAAGGATCGCTTGGTACGAGCACGTGAGGCCCTTGAAGTGCCCA<span class="snp"><tt>N</tt></span>GCGCA<span class="snp"><tt>N</tt></span> 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||<span class="snp"><tt>&nbsp;</tt></span>
6322R-2.in silico     61  GCCGCGCGCCAAGGATCGCTTGGTACGAGCACGTGAGGCCCTTGAAGTGCCCA<span class="snp"><tt>-</tt></span>GCGCA<span class="snp"><tt>-</tt></span> 120


6322R-2.IR_full       121 GCACGTGCCGGCCGAATGGTGGAGATGCAGAAGAAGCTCCAGTCGCTGGCTCCACTGTGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6322R-2.in silico     121 GCACGTGCCGGCCGAATGGTGGAGATGCAGAAGAAGCTCCAGTCGCTGGCTCCACTGTGC 180


6322R-2.IR_full       181 TCCCAGGTGGG<span class="snp"><tt>A</tt></span>TGACACACGGCCCGTGAGTAGTGCTGCCTTCAGTGAAGACTCCTCACT 240
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
6322R-2.in silico     181 TCCCAGGTGGG<span class="snp"><tt>-</tt></span>TGACACACGGCCCGTGAGTAGTGCTGCCTTCAGTGAAGACTCCTCACT 240


6322R-2.IR_full       241 CCTTCTGACCTCCTCATGGTCGGGTCTCTGCAAGCTGTGGAGCGTGCCCGATTGCGAGCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6322R-2.in silico     241 CCTTCTGACCTCCTCATGGTCGGGTCTCTGCAAGCTGTGGAGCGTGCCCGATTGCGAGCT 300


6322R-2.IR_full       301 GAAGCAGACGCTGCGTGGACATGCCAGCTATGTGGGCGGA<span class="snp"><tt>N</tt></span>GTGGCTCTGCGGCCGGGAG 360
                          ||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
6322R-2.in silico     301 GAAGCAGACGCTGCGTGGACATGCCAGCTATGTGGGCGGA<span class="snp"><tt>-</tt></span>GTGGCTCTGCGGCCGGGAG 360


6322R-2.IR_full       361 TTAAGGCAGACGAGGAGAACGTGGTGGCCATGGCTT<span class="snp"><tt>N</tt></span>CCGGTGGCCACGATGGCGCCGTC 420
                          ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
6322R-2.in silico     361 TTAAGGCAGACGAGGAGAACGTGGTGGCCATGGCTT<span class="snp"><tt>-</tt></span>CCGGTGGCCACGATGGCGCCGTC 420


6322R-2.IR_full       421 AAGCTGTGGGGCTTCAACAACGAAGAGTCGATTGCCGACATCACCGGCCACATGCCGCAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6322R-2.in silico     421 AAGCTGTGGGGCTTCAACAACGAAGAGTCGATTGCCGACATCACCGGCCACATGCCGCAT 480


6322R-2.IR_full       481 CGCGTC<span class="snp"><tt>N</tt></span>TCTAAGGTAGCCTTCCATCC 507
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||
6322R-2.in silico     481 CGCGTC<span class="snp"><tt>-</tt></span>TCTAAGGTAGCCTTCCATCC 507

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_140733.2  CG6322-RA (CG6322), mRNA 
0   0  4  4  3  NM_141156.2  CG11241-RA (CG11241), mRNA 
0   0  3  3  7  NM_001014543.1  CG6741-RC, transcript variant C (a), mRNA 
0   0  3  3  7  NM_166514.1  CG6741-RA, transcript variant A (a), mRNA 
0   0  3  3  7  NM_079902.2  CG6741-RB, transcript variant B (a), mRNA 
0   0  3  2  5  NM_001038864.1  CG4927-RC (CG4927), mRNA 
0   0  2  5  3  NM_169423.2  CG17342-RB, transcript variant B (Lk6), mRNA 
0   0  2  5  3  NM_143729.2  CG17342-RA, transcript variant A (Lk6), mRNA 
0   0  2  5  2  NM_142445.1  CG7146-RA (CG7146), mRNA 
0   0  2  4  2  NM_136616.3  CG8057-RA, transcript variant A (CG8057), mRNA 
0   0  2  4  2  NM_206061.1  CG8057-RB, transcript variant B (CG8057), mRNA 
0   0  2  3  5  NM_137946.2  CG10315-RA, transcript variant A (eIF2B-delta), mRNA 
0   0  2  3  5  NM_166602.1  CG10315-RC, transcript variant C (eIF2B-delta), mRNA 
0   0  2  3  5  NM_166601.1  CG10315-RB, transcript variant B (eIF2B-delta), mRNA 
0   0  1  6  3  NM_134987.1  CG15431-RA (CG15431), mRNA 
0   0  1  5  3  NM_079263.2  CG5263-RA, transcript variant A (smg), mRNA 
0   0  1  4  2  NM_079212.2  CG10578-RA, transcript variant A (DnaJ-1), mRNA 
0   0  1  4  2  NM_168128.1  CG10578-RB, transcript variant B (DnaJ-1), mRNA 
0   0  1  3  5  NM_176421.1  CG33191-RA (CG33191), mRNA 
0   0  1  3  3  NM_130494.2  CG13369-RA (CG13369), mRNA 
0   0  1  3  2  NM_140000.1  CG13310-RA (CG13310), mRNA 
0   0  1  3  2  NM_079017.2  CG8542-RA (Hsc70-5), mRNA 
0   0  1  3  2  NM_080323.2  CG10798-RA (dm), mRNA 
0   0  1  2  4  NM_136369.1  CG8330-RA (tomboy40), mRNA 
0   0  1  2  3  NM_136073.2  CG18397-RA (CG18397), mRNA 
0   0  1  2  3  NM_167080.1  CG32742-RA (l(1)G0148), mRNA 
0   0  1  2  2  NM_168413.1  CG32062-RB, transcript variant B (CG32062), mRNA 
0   0  1  2  2  NM_167972.1  CG32485-RA (CG32485), mRNA 
0   0  1  2  2  NM_168412.1  CG32062-RD, transcript variant D (CG32062), mRNA 
0   0  0  7  4  NM_140665.1  CG9674-RA, transcript variant A (CG9674), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme03040 : Spliceosome

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