NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6255R-1

Symbol

CG6255

Full Name

CG6255

CG No

CG6255

Old CG No

CG6255

Synonyms

AAF55672, anon-WO0140519.197, CG6255

Accession No (Link to NCBI)

NM_142552.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATCGAGGAAGCAAGATGCTC

Primer Seq. 3'

aatctagaggtaccCCGGCATTATTCCGATTTTGC

Predicted Fragment Size

IR fragment full Seq

0001 AATCGAGGAA GCAAGATGCT CCTGCGGGCG ATGACGCCCC AAATTTTGAG GCGTGGCAAG 
0061 GCGGACTATG GCAAGACGGT GTGCAACCTG AAGATCAACA AGGCAACAAA GGTTCTAGTC 
0121 CAGGGATTCA CCGGCAAGCA GGCCACTTTC CACTCGGAGG AGTCCATAAA GTACGGAACC 
0181 AATATAGTTG GCGGTGTGAA TNCCCAAAAA GGGAGGTACG GAGCATCTGG GCAAGCCCGT 
0241 ATTCAAATCG GTGGCAGAAG CTGTGGAGAA GGCGAAGCCA GATGCCACGG TGATCTTTAT 
0301 ACCACCTCCA TNCCGCCGCC GAGGGTATTT GCGCGGCTAT CGAGAGTGAA ATTGGCTTGA 
0361 TTGTGGCCAT CACCGAGGGT ATTCCCCAGG CGGATATGGT GCGGATATCG CAAATGCTGA 
0421 ATTGCCAGGA GAAGTCCCGC CTGCTGGGAC CCAACTGTCC GGGAATCATC TCGCCGGATC 
0481 AGTGCAAAAT CGGAATAATN CCGG

in silico PCR Fragment

0001 AATCGAGGAA GCAAGATGCT CCTGCGGGCG TTAACGCCCC AAATTTTGAG GCGTGGCAAG 
0061 GCGGACTATG GCAAGACGGT GTGCAACCTG AAGATCAACA AGGCAACAAA GGTTCTAGTC 
0121 CAGGGATTCA CCGGCAAGCA GGCCACTTTC CACTCGGAGG AGTCCATAAA GTACGGAACC 
0181 AATATAGTTG GCGGTGTGAA T-CCCAAAAA GGGAGGTACG GAGCATCTGG GCAAGCCCGT 
0241 ATTCAAATCG GTGGCAGAAG CTGTGGAGAA GGCGAAGCCA GATGCCACGG TGATCTTTAT 
0301 ACCACCTCCA T-CCGCCGCC GAGGGTATTT GCGCGGCTAT CGAGAGTGAA ATTGGCTTGA 
0361 TTGTGGCCAT CACCGAGGGT ATTCCCCAGG CGGATATGGT GCGGATATCG CAAATGCTGA 
0421 ATTGCCAGGA GAAGTCCCGC CTGCTGGGAC CCAACTGTCC GGGAATCATC TCGCCGGATC 
0481 AGTGCAAAAT CGGAATAATG CCGG

Assemble Data

<PRE>

6255R-1.IR_full       1   AATCGAGGAAGCAAGATGCTCCTGCGGGCG<span class="snp"><tt>A</tt></span>T<span class="snp"><tt>G</tt></span>ACGCCCCAAATTTTGAGGCGTGGCAAG 60
                          ||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||
6255R-1.in silico     1   AATCGAGGAAGCAAGATGCTCCTGCGGGCG<span class="snp"><tt>T</tt></span>T<span class="snp"><tt>A</tt></span>ACGCCCCAAATTTTGAGGCGTGGCAAG 60


6255R-1.IR_full       61  GCGGACTATGGCAAGACGGTGTGCAACCTGAAGATCAACAAGGCAACAAAGGTTCTAGTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6255R-1.in silico     61  GCGGACTATGGCAAGACGGTGTGCAACCTGAAGATCAACAAGGCAACAAAGGTTCTAGTC 120


6255R-1.IR_full       121 CAGGGATTCACCGGCAAGCAGGCCACTTTCCACTCGGAGGAGTCCATAAAGTACGGAACC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6255R-1.in silico     121 CAGGGATTCACCGGCAAGCAGGCCACTTTCCACTCGGAGGAGTCCATAAAGTACGGAACC 180


6255R-1.IR_full       181 AATATAGTTGGCGGTGTGAAT<span class="snp"><tt>N</tt></span>CCCAAAAAGGGAGGTACGGAGCATCTGGGCAAGCCCGT 240
                          |||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||
6255R-1.in silico     181 AATATAGTTGGCGGTGTGAAT<span class="snp"><tt>-</tt></span>CCCAAAAAGGGAGGTACGGAGCATCTGGGCAAGCCCGT 240


6255R-1.IR_full       241 ATTCAAATCGGTGGCAGAAGCTGTGGAGAAGGCGAAGCCAGATGCCACGGTGATCTTTAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6255R-1.in silico     241 ATTCAAATCGGTGGCAGAAGCTGTGGAGAAGGCGAAGCCAGATGCCACGGTGATCTTTAT 300


6255R-1.IR_full       301 ACCACCTCCAT<span class="snp"><tt>N</tt></span>CCGCCGCCGAGGGTATTTGCGCGGCTATCGAGAGTGAAATTGGCTTGA 360
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
6255R-1.in silico     301 ACCACCTCCAT<span class="snp"><tt>-</tt></span>CCGCCGCCGAGGGTATTTGCGCGGCTATCGAGAGTGAAATTGGCTTGA 360


6255R-1.IR_full       361 TTGTGGCCATCACCGAGGGTATTCCCCAGGCGGATATGGTGCGGATATCGCAAATGCTGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6255R-1.in silico     361 TTGTGGCCATCACCGAGGGTATTCCCCAGGCGGATATGGTGCGGATATCGCAAATGCTGA 420


6255R-1.IR_full       421 ATTGCCAGGAGAAGTCCCGCCTGCTGGGACCCAACTGTCCGGGAATCATCTCGCCGGATC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6255R-1.in silico     421 ATTGCCAGGAGAAGTCCCGCCTGCTGGGACCCAACTGTCCGGGAATCATCTCGCCGGATC 480


6255R-1.IR_full       481 AGTGCAAAATCGGAATAAT<span class="snp"><tt>N</tt></span>CCGG 504
                          |||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
6255R-1.in silico     481 AGTGCAAAATCGGAATAAT<span class="snp"><tt>G</tt></span>CCGG 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	484	0	0	3	NM_142552.2	CG6255-RA (CG6255), mRNA
0	0	9	12	35	NM_079181.2	CG1065-RA (Scsalpha), mRNA
0	0	4	3	4	NM_137070.1	CG16935-RA (CG16935), mRNA
0	0	3	5	3	NM_167642.1	CG7893-RB, transcript variant B (vav), mRNA
0	0	3	5	3	NM_133144.2	CG7893-RA, transcript variant A (vav), mRNA
0	0	3	3	2	NM_142147.2	CG7262-RA (CG7262), mRNA
0	0	2	3	2	NM_164684.1	CG31641-RA, transcript variant A (stai), mRNA
0	0	2	3	2	NM_164682.1	CG31641-RC, transcript variant C (stai), mRNA
0	0	2	3	2	NM_164683.1	CG31641-RB, transcript variant B (stai), mRNA
0	0	2	2	3	NM_142149.1	CG7026-RA (CG7026), mRNA
0	0	1	4	6	NM_141847.2	CG6834-RA (CG6834), mRNA
0	0	1	4	2	NM_137793.1	CG13502-RA, transcript variant A (CG13502), mRNA
0	0	1	4	2	NM_166506.1	CG13502-RB, transcript variant B (CG13502), mRNA
0	0	1	3	6	NM_079229.1	CG8624-RA, transcript variant A (melt), mRNA
0	0	1	3	6	NM_001014572.1	CG8624-RB, transcript variant B (melt), mRNA
0	0	1	3	2	NM_137187.2	CG8160-RA (CG8160), mRNA
0	0	1	2	7	NM_057266.4	CG4162-RA (lace), mRNA
0	0	1	2	3	NM_136250.2	CG9247-RA (CG9247), mRNA
0	0	1	2	2	NM_164887.1	CG31714-RA (CG31714), mRNA
0	0	0	5	4	NM_134605.2	CG1696-RA (l(1)G0269), mRNA
0	0	0	5	4	NM_001043266.1	CG34119-RA (CG34119), mRNA
0	0	0	4	5	NM_142783.2	CG13849-RA (Nop56), mRNA
0	0	0	4	4	NM_169857.1	CG31244-RA (CG31244), mRNA
0	0	0	4	2	NM_166332.1	CG10737-RB, transcript variant B (CG10737), mRNA
0	0	0	4	2	NM_166334.1	CG10737-RA, transcript variant A (CG10737), mRNA
0	0	0	4	2	NM_166333.1	CG10737-RD, transcript variant D (CG10737), mRNA
0	0	0	4	2	NM_001014538.1	CG10737-RE, transcript variant E (CG10737), mRNA
0	0	0	4	2	NM_137554.2	CG10737-RC, transcript variant C (CG10737), mRNA
0	0	0	3	7	NM_057507.3	CG8442-RA (Glu-RI), mRNA
0	0	0	3	6	NM_139612.1	CG1308-RA (CG1308), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00020 : Citrate cycle (TCA cycle)
dme00640 : Propanoate metabolism
dme01100 : Metabolic pathways
dme01200 : Carbon metabolism

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