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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6168R-8

Symbol

CG6168

Full Name

CG6168

CG No

CG6168

Old CG No

CG6168

Synonyms

CG6168

Accession No (Link to NCBI)

NM_140202.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCACCGGGACATTCGGAAGTGA

Primer Seq. 3'

aatctagaggtaccGAACCAGCCTGATGTATCGAG

Predicted Fragment Size

IR fragment full Seq

0001 CACCgGNACA TTCGGAAGTG AGAAACTACA TAGTGGATTA CCTAAAGAAA CTGAACTGGA 
0061 ACGTAGAGTT AGATATATTT ACGCAGAAAG TGCCTATTAT GAGTAATGTA ACCTTTCACA 
0121 ATATTGTGGC CCGGCAAAAT CCTCAAACCC AAAGATACCT AATGTTTGGC TGCCACTATG 
0181 ACAGCAAGTA TTTCAAGGAT TTCGATTTTA TGGCCGCCAC GGATTCGGCA GTTCCCTGTG 
0241 CACTAATGTT AAATATGGCT ACAATTTTGA AACACCAGTT TCATCGCTCC CAGGTCAGTT 
0301 TAATGCTGGT CTTCTTCGAC GGTGAGGAGG CATTTGGGGA ATGGTCGCAG GAGGATTCGC 
0361 CATATGGTTC CAGACATTTG GCGGAGCTGT GGGAGAAGCA CGGTTTTCTT GATAAGATAG 
0421 ACCTCTTCGT GCTGCCGGAT CTAATTGGAG CCAAGGATGT GGTCTTTAAG AAAAACATTC 
0481 TCGATACATC AGGCTGGTTC

in silico PCR Fragment

0001 CACCGGGACA TTCGGAAGTG AGAAACTACA TAGTGGATTA CCTAAAGAAA CTGAACTGGA 
0061 ACGTAGAGTT AGATATATTT ACGCAGAAAG TGCCTATTAT GAGTAATGTA ACCTTTCACA 
0121 ATATTGTGGC CCGGCAAAAT CCTCAAACCC AAAGATACCT AATGTTTGGC TGCCACTATG 
0181 ACAGCAAGTA TTTCAAGGAT TTCGATTTTA TGGCCGCCAC GGATTCGGCA GTTCCCTGTG 
0241 CACTAATGTT AAATATGGCT ACAATTTTGA AACACCAGTT TCATCGCTCC CAGGTCAGTT 
0301 TAATGCTGGT CTTCTTCGAC GGTGAGGAGG CATTTGGGGA ATGGTCGCAG GAGGATTCGC 
0361 CATATGGTTC CAGACATTTG GCGGAGCTGT GGGAGAAGCA CGGTTTTCTT GATAAGATAG 
0421 ACCTCTTCGT GCTGCCGGAT CTAATTGGAG CCAAGGATGT GGTCTTTAAG AAAAACATTC 
0481 TCGATACATC AGGCTGGTTC

Assemble Data

<PRE>

6168R-8.IR_full       1   CACCGG<span class="snp"><tt>N</tt></span>ACATTCGGAAGTGAGAAACTACATAGTGGATTACCTAAAGAAACTGAACTGGA 60
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     1   CACCGG<span class="snp"><tt>G</tt></span>ACATTCGGAAGTGAGAAACTACATAGTGGATTACCTAAAGAAACTGAACTGGA 60


6168R-8.IR_full       61  ACGTAGAGTTAGATATATTTACGCAGAAAGTGCCTATTATGAGTAATGTAACCTTTCACA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     61  ACGTAGAGTTAGATATATTTACGCAGAAAGTGCCTATTATGAGTAATGTAACCTTTCACA 120


6168R-8.IR_full       121 ATATTGTGGCCCGGCAAAATCCTCAAACCCAAAGATACCTAATGTTTGGCTGCCACTATG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     121 ATATTGTGGCCCGGCAAAATCCTCAAACCCAAAGATACCTAATGTTTGGCTGCCACTATG 180


6168R-8.IR_full       181 ACAGCAAGTATTTCAAGGATTTCGATTTTATGGCCGCCACGGATTCGGCAGTTCCCTGTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     181 ACAGCAAGTATTTCAAGGATTTCGATTTTATGGCCGCCACGGATTCGGCAGTTCCCTGTG 240


6168R-8.IR_full       241 CACTAATGTTAAATATGGCTACAATTTTGAAACACCAGTTTCATCGCTCCCAGGTCAGTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     241 CACTAATGTTAAATATGGCTACAATTTTGAAACACCAGTTTCATCGCTCCCAGGTCAGTT 300


6168R-8.IR_full       301 TAATGCTGGTCTTCTTCGACGGTGAGGAGGCATTTGGGGAATGGTCGCAGGAGGATTCGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     301 TAATGCTGGTCTTCTTCGACGGTGAGGAGGCATTTGGGGAATGGTCGCAGGAGGATTCGC 360


6168R-8.IR_full       361 CATATGGTTCCAGACATTTGGCGGAGCTGTGGGAGAAGCACGGTTTTCTTGATAAGATAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     361 CATATGGTTCCAGACATTTGGCGGAGCTGTGGGAGAAGCACGGTTTTCTTGATAAGATAG 420


6168R-8.IR_full       421 ACCTCTTCGTGCTGCCGGATCTAATTGGAGCCAAGGATGTGGTCTTTAAGAAAAACATTC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-8.in silico     421 ACCTCTTCGTGCTGCCGGATCTAATTGGAGCCAAGGATGTGGTCTTTAAGAAAAACATTC 480


6168R-8.IR_full       481 TCGATACATCAGGCTGGTTC 500
                          ||||||||||||||||||||
6168R-8.in silico     481 TCGATACATCAGGCTGGTTC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140202.1	CG6168-RB (CG6168), mRNA
0	0	5	13	11	NM_168145.1	CG32412-RA (CG32412), mRNA
0	0	3	3	3	NM_142975.2	CG5669-RA (CG5669), mRNA
0	0	2	4	4	NM_001042804.1	CG2174-RB, transcript variant B (Myo10A), mRNA
0	0	2	4	4	NM_132441.1	CG2174-RA, transcript variant A (Myo10A), mRNA
0	0	2	4	3	NM_141057.2	CG5664-RA (CG5664), mRNA
0	0	2	2	4	NM_169801.1	CG31241-RA (CG31241), mRNA
0	0	2	2	2	NM_170213.2	CG31102-RA (CG31102), mRNA
0	0	1	4	4	NM_144348.2	CG12135-RA (c12.1), mRNA
0	0	1	3	5	NM_079411.2	CG7347-RA (mus304), mRNA
0	0	1	3	2	NM_001015393.1	CG41123-PA (CG41123), mRNA
0	0	1	3	2	NM_132621.1	CG12715-RA (CG12715), mRNA
0	0	1	2	6	NM_142869.3	CG4656-RA (CG4656), mRNA
0	0	1	2	5	NM_142459.2	CG7671-RA (CG7671), mRNA
0	0	1	2	4	NM_132300.2	CG12664-RB (ld14), mRNA
0	0	1	2	4	NM_132683.1	CG12177-RA (CG12177), mRNA
0	0	1	2	3	NM_132056.3	CG4790-RA (fs(1)M3), mRNA
0	0	1	2	2	NM_001042799.1	CG10966-RB, transcript variant B (rdgA), mRNA
0	0	1	2	2	NM_142581.1	CG4562-RA (CG4562), mRNA
0	0	1	2	2	NM_079920.2	CG6727-RA (gom), mRNA
0	0	1	2	2	NM_169522.1	CG8863-RE, transcript variant E (CG8863), mRNA
0	0	0	5	5	NM_169715.1	CG17931-RA, transcript variant A (CG17931), mRNA
0	0	0	5	2	NM_166779.2	CG1732-RB, transcript variant B (CG1732), mRNA
0	0	0	5	2	NM_143673.1	CG1732-RA, transcript variant A (CG1732), mRNA
0	0	0	4	25	NM_176373.1	CG5976-RA, transcript variant A (CG5976), mRNA
0	0	0	4	25	NM_176372.1	CG5976-RB, transcript variant B (CG5976), mRNA
0	0	0	4	12	NM_206265.1	CG12008-RC, transcript variant C (kst), mRNA
0	0	0	4	12	NM_206266.1	CG12008-RB, transcript variant B (kst), mRNA
0	0	0	4	12	NM_079176.1	CG12008-RA, transcript variant A (kst), mRNA
0	0	0	4	7	NM_135786.1	CG6108-RA (CG6108), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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