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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6168R-2 |
| Symbol |
CG6168 |
Full Name |
CG6168 |
| CG No |
CG6168 |
Old CG No |
CG6168 |
| Synonyms |
CG6168 |
| Accession No (Link to NCBI) |
NM_140202.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
2 lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACCGGGACATTCGGAAGTGA |
| Primer Seq. 3' |
aatctagaggtaccGAACCAGCCTGATGTATCGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACCgGNACA TTCGGAAGTG AGAAACTACA TAGTGGATTA CCTAAAGAAA CTGAACTGGA
0061 ACGTAGAGTT AGATATATTT ACGCAGAAAG TGCCTATTAT GAGTAATGTA ACCTTTCACA
0121 ATATTGTGGC CCGGCAAAAT CCTCAAACCC AAAGATACCT AATGTTTGGC TGCCACTATG
0181 ACAGCAAGTA TTTCAAGGAT TTCGATTTTA TGGCCGCCAC GGATTCGGCA GTTCCCTGTG
0241 CACTAATGTT AAATATGGCT ACAATTTTGA AACACCAGTT TCATCGCTCC CAGGTCAGTT
0301 TAATGCTGGT CTTCTTCGAC GGTGAGGAGG CATTTGGGGA ATGGTCGCAG GAGGATTCGC
0361 CATATGGTTC CAGACATTTG GCGGAGCTGT GGGAGAAGCA CGGTTTTCTT GATAAGATAG
0421 ACCTCTTCGT GCTGCCGGAT CTAATTGGAG CCAAGGATGT GGTCTTTAAG AAAAACATTC
0481 TCGATACATC AGGCTGGTTC |
| in silico PCR Fragment |
0001 CACCGGGACA TTCGGAAGTG AGAAACTACA TAGTGGATTA CCTAAAGAAA CTGAACTGGA
0061 ACGTAGAGTT AGATATATTT ACGCAGAAAG TGCCTATTAT GAGTAATGTA ACCTTTCACA
0121 ATATTGTGGC CCGGCAAAAT CCTCAAACCC AAAGATACCT AATGTTTGGC TGCCACTATG
0181 ACAGCAAGTA TTTCAAGGAT TTCGATTTTA TGGCCGCCAC GGATTCGGCA GTTCCCTGTG
0241 CACTAATGTT AAATATGGCT ACAATTTTGA AACACCAGTT TCATCGCTCC CAGGTCAGTT
0301 TAATGCTGGT CTTCTTCGAC GGTGAGGAGG CATTTGGGGA ATGGTCGCAG GAGGATTCGC
0361 CATATGGTTC CAGACATTTG GCGGAGCTGT GGGAGAAGCA CGGTTTTCTT GATAAGATAG
0421 ACCTCTTCGT GCTGCCGGAT CTAATTGGAG CCAAGGATGT GGTCTTTAAG AAAAACATTC
0481 TCGATACATC AGGCTGGTTC |
| Assemble Data |
<PRE>
6168R-2.IR_full 1 CACCGG<span class="snp"><tt>N</tt></span>ACATTCGGAAGTGAGAAACTACATAGTGGATTACCTAAAGAAACTGAACTGGA 60
||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 1 CACCGG<span class="snp"><tt>G</tt></span>ACATTCGGAAGTGAGAAACTACATAGTGGATTACCTAAAGAAACTGAACTGGA 60
6168R-2.IR_full 61 ACGTAGAGTTAGATATATTTACGCAGAAAGTGCCTATTATGAGTAATGTAACCTTTCACA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 61 ACGTAGAGTTAGATATATTTACGCAGAAAGTGCCTATTATGAGTAATGTAACCTTTCACA 120
6168R-2.IR_full 121 ATATTGTGGCCCGGCAAAATCCTCAAACCCAAAGATACCTAATGTTTGGCTGCCACTATG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 121 ATATTGTGGCCCGGCAAAATCCTCAAACCCAAAGATACCTAATGTTTGGCTGCCACTATG 180
6168R-2.IR_full 181 ACAGCAAGTATTTCAAGGATTTCGATTTTATGGCCGCCACGGATTCGGCAGTTCCCTGTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 181 ACAGCAAGTATTTCAAGGATTTCGATTTTATGGCCGCCACGGATTCGGCAGTTCCCTGTG 240
6168R-2.IR_full 241 CACTAATGTTAAATATGGCTACAATTTTGAAACACCAGTTTCATCGCTCCCAGGTCAGTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 241 CACTAATGTTAAATATGGCTACAATTTTGAAACACCAGTTTCATCGCTCCCAGGTCAGTT 300
6168R-2.IR_full 301 TAATGCTGGTCTTCTTCGACGGTGAGGAGGCATTTGGGGAATGGTCGCAGGAGGATTCGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 301 TAATGCTGGTCTTCTTCGACGGTGAGGAGGCATTTGGGGAATGGTCGCAGGAGGATTCGC 360
6168R-2.IR_full 361 CATATGGTTCCAGACATTTGGCGGAGCTGTGGGAGAAGCACGGTTTTCTTGATAAGATAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 361 CATATGGTTCCAGACATTTGGCGGAGCTGTGGGAGAAGCACGGTTTTCTTGATAAGATAG 420
6168R-2.IR_full 421 ACCTCTTCGTGCTGCCGGATCTAATTGGAGCCAAGGATGTGGTCTTTAAGAAAAACATTC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6168R-2.in silico 421 ACCTCTTCGTGCTGCCGGATCTAATTGGAGCCAAGGATGTGGTCTTTAAGAAAAACATTC 480
6168R-2.IR_full 481 TCGATACATCAGGCTGGTTC 500
||||||||||||||||||||
6168R-2.in silico 481 TCGATACATCAGGCTGGTTC 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140202.1 |
CG6168-RB (CG6168), mRNA |
| 0 |
0 |
5 |
13 |
11 |
NM_168145.1 |
CG32412-RA (CG32412), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_142975.2 |
CG5669-RA (CG5669), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_001042804.1 |
CG2174-RB, transcript variant B (Myo10A), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_132441.1 |
CG2174-RA, transcript variant A (Myo10A), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_141057.2 |
CG5664-RA (CG5664), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169801.1 |
CG31241-RA (CG31241), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_170213.2 |
CG31102-RA (CG31102), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_144348.2 |
CG12135-RA (c12.1), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079411.2 |
CG7347-RA (mus304), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001015393.1 |
CG41123-PA (CG41123), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132621.1 |
CG12715-RA (CG12715), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_142869.3 |
CG4656-RA (CG4656), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_142459.2 |
CG7671-RA (CG7671), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132300.2 |
CG12664-RB (ld14), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132683.1 |
CG12177-RA (CG12177), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132056.3 |
CG4790-RA (fs(1)M3), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001042799.1 |
CG10966-RB, transcript variant B (rdgA), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142581.1 |
CG4562-RA (CG4562), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079920.2 |
CG6727-RA (gom), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169522.1 |
CG8863-RE, transcript variant E (CG8863), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_169715.1 |
CG17931-RA, transcript variant A (CG17931), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_166779.2 |
CG1732-RB, transcript variant B (CG1732), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_143673.1 |
CG1732-RA, transcript variant A (CG1732), mRNA |
| 0 |
0 |
0 |
4 |
25 |
NM_176373.1 |
CG5976-RA, transcript variant A (CG5976), mRNA |
| 0 |
0 |
0 |
4 |
25 |
NM_176372.1 |
CG5976-RB, transcript variant B (CG5976), mRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_206265.1 |
CG12008-RC, transcript variant C (kst), mRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_206266.1 |
CG12008-RB, transcript variant B (kst), mRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_079176.1 |
CG12008-RA, transcript variant A (kst), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_135786.1 |
CG6108-RA (CG6108), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| +- |
|
| DC |
 |
| +- |
|
| DM |
 |
| +- |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
