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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6128R-3 
 Symbol Fuca  Full Name alpha-L-fucosidase 
 CG No CG6128  Old CG No CG6128 
 Synonyms CG33047, Fuca, Fuca_Dm, CG6128 
 Accession No (Link to NCBI)  
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTGGACCAGAGACCATTGCCC 
 Primer Seq. 3' 
aatctagaggtaccCGCTCCACAAAGTGCTGTTGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTGGACCAGA GACCATTGCC CCAATGGTAC GACGATGCCA AGGTGGGCAT CTTTCTGCAC 
0061 TACGGCGTTT ACTCGGTGCC CAGTTTTGGT TCCGAGTGGT TCTGGACCAA CTGGAAAAAT 
0121 CTTCGCAATC CAGAGTACGT CCAATTCATG CAGCGTAATT ACAAGCCCGA CTTTACTTAT 
0181 CAGGAATTTG CCAGTCAATT CACAGCGGAG CTATTCAATG CCACCAAGTG GGCGTTACTT 
0241 TTCAAAGATA GCGGAGCCAG ATATGTTGTG CTCACCAGCA AACATCACGA TGGCTTCACG 
0301 CTGTGGCCCT CGAAGAATAG CTACGGATGG AATTCCATGG ATGTGGGACC CAAACGGGAT 
0361 ATAGTCAAGG AANCT--CTG CCGCAATTCG AAAGGAGTCC GATCTCCGCT TCGGACTCTA 
0421 CTATTCTCTA TTCGAATGGT TCAACCCACT GTGGACCGAT GACAAATTGC ATTTGTTGAT 
0481 GCAACAGCAC TTTGTGGA  
 in silico PCR Fragment 
0001 ttggaccaga gaccattgcc ccaatggtac gacgatgcca aggtgggcat ctttctgcac 
0061 tacggcgttt actcggtgcc cagttttggt tccgagtggt tctggaccaa ctggaaaaat 
0121 cttcgcaatc cagagtacgt ccaattcatg cagcgtaatt acaagcccga cttcacttat 
0181 caggaatttg ccagtcaatt cacagcggag ctattcaatg ccaccaagtg ggcgttactt 
0241 ttcaaagata gcggagccag atatgttgtg ctcaccagca aacatcacga tggcttcacg 
0301 ctgtggccct cgaagaatag ctacggatgg aattccatgg atgtgggacc caaacgagat 
0361 atagtcaagg aactggctgc cgcaattcga aaggagtccg atcttcgttt tggactctat 
0421 tattctctat tcgaatggtt caacccactg tggaccgatg acaaattgca tctgttaatg 
0481 caacagcact ttgtggagcg  
 Assemble Data 
<PRE>

6128R-3.IR full       1   TTGGACCAGAGACCATTGCCCCAATGGTACGACGATGCCAAGGTGGGCATCTTTCTGCAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-3.in silico     1   TTGGACCAGAGACCATTGCCCCAATGGTACGACGATGCCAAGGTGGGCATCTTTCTGCAC 60


6128R-3.IR full       61  TACGGCGTTTACTCGGTGCCCAGTTTTGGTTCCGAGTGGTTCTGGACCAACTGGAAAAAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-3.in silico     61  TACGGCGTTTACTCGGTGCCCAGTTTTGGTTCCGAGTGGTTCTGGACCAACTGGAAAAAT 120


6128R-3.IR full       121 CTTCGCAATCCAGAGTACGTCCAATTCATGCAGCGTAATTACAAGCCCGACTT<span class="snp"><tt>T</tt></span>ACTTAT 180
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
6128R-3.in silico     121 CTTCGCAATCCAGAGTACGTCCAATTCATGCAGCGTAATTACAAGCCCGACTT<span class="snp"><tt>C</tt></span>ACTTAT 180


6128R-3.IR full       181 CAGGAATTTGCCAGTCAATTCACAGCGGAGCTATTCAATGCCACCAAGTGGGCGTTACTT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-3.in silico     181 CAGGAATTTGCCAGTCAATTCACAGCGGAGCTATTCAATGCCACCAAGTGGGCGTTACTT 240


6128R-3.IR full       241 TTCAAAGATAGCGGAGCCAGATATGTTGTGCTCACCAGCAAACATCACGATGGCTTCACG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-3.in silico     241 TTCAAAGATAGCGGAGCCAGATATGTTGTGCTCACCAGCAAACATCACGATGGCTTCACG 300


6128R-3.IR full       301 CTGTGGCCCTCGAAGAATAGCTACGGATGGAATTCCATGGATGTGGGACCCAAACG<span class="snp"><tt>G</tt></span>GAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
6128R-3.in silico     301 CTGTGGCCCTCGAAGAATAGCTACGGATGGAATTCCATGGATGTGGGACCCAAACG<span class="snp"><tt>A</tt></span>GAT 360


6128R-3.IR full       361 ATAGTCAAGGAA<span class="snp"><tt>N</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CTGCCGCAATTCGAAAGGAGTCCGATCT<span class="snp"><tt>C</tt></span>CG<span class="snp"><tt>C</tt></span>TT<span class="snp"><tt>C</tt></span>GGACTCTA 420
                          ||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||||
6128R-3.in silico     361 ATAGTCAAGGAA<span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>-</tt></span>CTGCCGCAATTCGAAAGGAGTCCGATCT<span class="snp"><tt>T</tt></span>CG<span class="snp"><tt>T</tt></span>TT<span class="snp"><tt>T</tt></span>GGACTCTA 420


6128R-3.IR full       421 <span class="snp"><tt>C</tt></span>TATTCTCTATTCGAATGGTTCAACCCACTGTGGACCGATGACAAATTGCAT<span class="snp"><tt>T</tt></span>TGTT<span class="snp"><tt>G</tt></span>AT 480
                          <span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>||
6128R-3.in silico     421 <span class="snp"><tt>T</tt></span>TATTCTCTATTCGAATGGTTCAACCCACTGTGGACCGATGACAAATTGCAT<span class="snp"><tt>C</tt></span>TGTT<span class="snp"><tt>A</tt></span>AT 480


6128R-3.IR full       481 GCAACAGCACTTTGTGGA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span> 501
                          ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>
6128R-3.in silico     481 GCAACAGCACTTTGTGGA<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span> 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100  482  0  0  0  NM_001031952.1  CG11714-RA, transcript variant A (CG11714), mRNA 
100  482  0  0  0  NM_001031953.1  CG6128-RA, transcript variant A (CG6128), mRNA 
100  482  0  0  0  NM_140215.1  CG6128-RB, transcript variant B (CG6128), mRNA 
0.2  1  2  3  2  NM_058053.3  SET domain binding factor CG6939-RA, transcript variant A (Sbf), mRNA 
0.2  1  2  3  2  NM_169430.2  SET domain binding factor CG6939-RB, transcript variant B (Sbf), mRNA 
0  0  2  2  3  NM_137499.2  CG30115-RC, transcript variant C (CG30115), mRNA 
0  0  2  2  3  NM_176223.2  CG30115-RD, transcript variant D (CG30115), mRNA 
0  0  1  5  2  NM_169222.2  CG31462-RA (CG31462), mRNA 
0  0  1  4  3  NM_132058.2  CG5966-RA (CG5966), mRNA 
0  0  1  3  5  NM_142220.1  CG6045-RA (CG6045), mRNA 
0  0  1  3  3  NM_143585.2  CG11318-RA (CG11318), mRNA 
0  0  1  2  4  NM_140488.2  CG6945-RA (CG6945), mRNA 
0  0  1  2  2  NM_164439.2  Gustatory receptor 22c CG31929-RA (Gr22c), mRNA 
0  0  1  2  2  NM_137586.1  CG15121-RA (CG15121), mRNA 
0  0  1  2  2  NM_142599.1  CG4836-RC, transcript variant C (CG4836), mRNA 
0  0  1  2  2  NM_169883.1  CG4836-RA, transcript variant A (CG4836), mRNA 
0  0  1  2  2  NM_169882.1  CG4836-RB, transcript variant B (CG4836), mRNA 
0  0  0  7  2  NM_132956.1  CG9086-RA (CG9086), mRNA 
0  0  0  5  6  NM_166846.3  silver CG4122-RA, transcript variant A (svr), mRNA 
0  0  0  5  6  NM_206599.1  silver CG4122-RD, transcript variant D (svr), mRNA 
0  0  0  5  6  NM_080293.3  silver CG4122-RB, transcript variant B (svr), mRNA 
0  0  0  5  6  NM_206596.1  silver CG4122-RG, transcript variant G (svr), mRNA 
0  0  0  5  6  NM_176679.2  silver CG4122-RC, transcript variant C (svr), mRNA 
0  0  0  4  6  NM_136951.2  CG8569-RA (CG8569), mRNA 
0  0  0  4  2  NM_141731.1  CG3996-RA (CG3996), mRNA 
0  0  0  4  2  NM_135224.1  CG11201-RA (CG11201), mRNA 
0  0  0  3  17  NM_001032052.1  Muscle-specific protein 300 CG33715-RE, transcript variant E (Msp-300), mRNA 
0  0  0  3  7  NM_142221.1  CG18516-RA (CG18516), mRNA 
0  0  0  3  7  NM_057737.2  Dynein heavy chain at 62B CG15804-RA, transcript variant A (Dhc62B), mRNA 
0  0  0  3  7  NM_206236.1  Dynein heavy chain at 62B CG15804-RB, transcript variant B (Dhc62B), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
+ 
DB
++ 
DC
++ 
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
++ 
DC
++ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
+     
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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