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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6128R-1 |
| Symbol |
Fuca |
Full Name |
alpha-L-fucosidase |
| CG No |
CG6128 |
Old CG No |
CG6128 |
| Synonyms |
CG33047, Fuca, Fuca_Dm, CG6128 |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGGACCAGAGACCATTGCCC |
| Primer Seq. 3' |
aatctagaggtaccCGCTCCACAAAGTGCTGTTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
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| in silico PCR Fragment |
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| Assemble Data |
<PRE>
6128R-1.IR full 1 TTGGACCAGAGACCATTGCCCCAATGGTACGACGATGCCAAGGTGGGCATCTTTCTGCAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-1.in silico 1 TTGGACCAGAGACCATTGCCCCAATGGTACGACGATGCCAAGGTGGGCATCTTTCTGCAC 60
6128R-1.IR full 61 TACGGCGTTTACTCGGTGCCCAGTTTTGGTTCCGAGTGGTTCTGGACCAACTGGAAAAAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-1.in silico 61 TACGGCGTTTACTCGGTGCCCAGTTTTGGTTCCGAGTGGTTCTGGACCAACTGGAAAAAT 120
6128R-1.IR full 121 CTTCGCAATCCAGAGTACGTCCAATTCATGCAGCGTAATTACAAGCCCGACTT<span class="snp"><tt>T</tt></span>ACTTAT 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||
6128R-1.in silico 121 CTTCGCAATCCAGAGTACGTCCAATTCATGCAGCGTAATTACAAGCCCGACTT<span class="snp"><tt>C</tt></span>ACTTAT 180
6128R-1.IR full 181 CAGGAATTTGCCAGTCAATTCACAGCGGAGCTATTCAATGCCACCAAGTGGGCGTTACTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-1.in silico 181 CAGGAATTTGCCAGTCAATTCACAGCGGAGCTATTCAATGCCACCAAGTGGGCGTTACTT 240
6128R-1.IR full 241 TTCAAAGATAGCGGAGCCAGATATGTTGTGCTCACCAGCAAACATCACGATGGCTTCACG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6128R-1.in silico 241 TTCAAAGATAGCGGAGCCAGATATGTTGTGCTCACCAGCAAACATCACGATGGCTTCACG 300
6128R-1.IR full 301 CTGTGGCCCTCGAAGAATAGCTACGGATGGAATTCCATGGATGTGGGACCCAAACG<span class="snp"><tt>G</tt></span>GAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||
6128R-1.in silico 301 CTGTGGCCCTCGAAGAATAGCTACGGATGGAATTCCATGGATGTGGGACCCAAACG<span class="snp"><tt>A</tt></span>GAT 360
6128R-1.IR full 361 ATAGTCAAGGAA<span class="snp"><tt>N</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CTGCCGCAATTCGAAAGGAGTCCGATCT<span class="snp"><tt>C</tt></span>CG<span class="snp"><tt>C</tt></span>TT<span class="snp"><tt>C</tt></span>GGACTCTA 420
||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>||||||||
6128R-1.in silico 361 ATAGTCAAGGAA<span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>-</tt></span>CTGCCGCAATTCGAAAGGAGTCCGATCT<span class="snp"><tt>T</tt></span>CG<span class="snp"><tt>T</tt></span>TT<span class="snp"><tt>T</tt></span>GGACTCTA 420
6128R-1.IR full 421 <span class="snp"><tt>C</tt></span>TATTCTCTATTCGAATGGTTCAACCCACTGTGGACCGATGACAAATTGCAT<span class="snp"><tt>T</tt></span>TGTT<span class="snp"><tt>G</tt></span>AT 480
<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>||
6128R-1.in silico 421 <span class="snp"><tt>T</tt></span>TATTCTCTATTCGAATGGTTCAACCCACTGTGGACCGATGACAAATTGCAT<span class="snp"><tt>C</tt></span>TGTT<span class="snp"><tt>A</tt></span>AT 480
6128R-1.IR full 481 GCAACAGCACTTTGTGGA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span> 501
||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>
6128R-1.in silico 481 GCAACAGCACTTTGTGGA<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span> 501
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_001031952.1 |
CG11714-RA, transcript variant A (CG11714), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_001031953.1 |
CG6128-RA, transcript variant A (CG6128), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_140215.1 |
CG6128-RB, transcript variant B (CG6128), mRNA |
| 0.2 |
1 |
2 |
3 |
2 |
NM_058053.3 |
SET domain binding factor CG6939-RA, transcript variant A (Sbf), mRNA |
| 0.2 |
1 |
2 |
3 |
2 |
NM_169430.2 |
SET domain binding factor CG6939-RB, transcript variant B (Sbf), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137499.2 |
CG30115-RC, transcript variant C (CG30115), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_176223.2 |
CG30115-RD, transcript variant D (CG30115), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_169222.2 |
CG31462-RA (CG31462), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132058.2 |
CG5966-RA (CG5966), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_142220.1 |
CG6045-RA (CG6045), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_143585.2 |
CG11318-RA (CG11318), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140488.2 |
CG6945-RA (CG6945), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164439.2 |
Gustatory receptor 22c CG31929-RA (Gr22c), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137586.1 |
CG15121-RA (CG15121), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142599.1 |
CG4836-RC, transcript variant C (CG4836), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169883.1 |
CG4836-RA, transcript variant A (CG4836), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169882.1 |
CG4836-RB, transcript variant B (CG4836), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_132956.1 |
CG9086-RA (CG9086), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_166846.3 |
silver CG4122-RA, transcript variant A (svr), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_206599.1 |
silver CG4122-RD, transcript variant D (svr), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_080293.3 |
silver CG4122-RB, transcript variant B (svr), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_206596.1 |
silver CG4122-RG, transcript variant G (svr), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_176679.2 |
silver CG4122-RC, transcript variant C (svr), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_136951.2 |
CG8569-RA (CG8569), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141731.1 |
CG3996-RA (CG3996), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135224.1 |
CG11201-RA (CG11201), mRNA |
| 0 |
0 |
0 |
3 |
17 |
NM_001032052.1 |
Muscle-specific protein 300 CG33715-RE, transcript variant E (Msp-300), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_142221.1 |
CG18516-RA (CG18516), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_057737.2 |
Dynein heavy chain at 62B CG15804-RA, transcript variant A (Dhc62B), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_206236.1 |
Dynein heavy chain at 62B CG15804-RB, transcript variant B (Dhc62B), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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