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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6117R-2 |
| Symbol |
Pka-C3 |
Full Name |
cAMP-dependent protein kinase 3 |
| CG No |
CG6117 |
Old CG No |
CG6117 |
| Synonyms |
PKA-C-3, CG6117, PKA, PKA C3, DC2, 5-23, Pk?5, Pk72A, Dpck, Pka-C3 |
| Accession No (Link to NCBI) |
NM_079373.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ibaraki K, Nakatsuka M, Ohsako T, Watanabe M, Miyazaki Y, Shirakami M, Karr TL, Sanuki R, Tomaru M, Takano-Shimizu-Kouno T.<br> A cross-species approach for the identification of Drosophila male sterility genes.<br> G3 (Bethesda) (2021) 11(8)
[
PubMed ID = 34849808
]
[
RRC reference
]
Cassar M, Sunderhaus E, Wentzell JS, Kuntz S, Strauss R, Kretzschmar D.<br> The PKA-C3 catalytic subunit is required in two pairs of interneurons for successful mating of Drosophila.<br> Sci Rep (2018) 8(1) 2458
[
PubMed ID = 29410515
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.<br> Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.<br> Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Wentzell JS, Cassar M, Kretzschmar D.<br> Organophosphate-induced changes in the PKA regulatory function of Swiss Cheese/NTE lead to behavioral deficits and neurodegeneration.<br> PLoS One (2014) 9(2) e87526
[
PubMed ID = 24558370
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGACAACCGGAAACGGAAGCG |
| Primer Seq. 3' |
aatctagaggtaccGTCGTCCAAGTGGTATTTGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGACAACCGG AAACGGAAGC GGCAACACGA TGACGTCGGC GTACAAGAAG AAAATTCCAT
0061 CAAACAACAG CACAACAGCA AATGACAGCA GCAACACAGA GACAACATTC ACATTCAAAT
0121 TGGGCCGCTC GAACGGCCGA AGTAGCAGCA ATGTAGCCTC AAGCGAGTCA TCCGATCCAC
0181 TGGAGTCCGA TTACAGCGAG GAGGATCCGG AGCGAGGAGC AGCAGCGACC CGATcCGGCG
0241 ACCAACAGNC GGAGCAGCAG CACCGCCACC ACCACCACCA CCAGTTCCGC TGACCATGAC
0301 AGTGACGTCG ACGAGGAGGA TGAGGAGGAC GACGAGAACG AGGGGGAGGG CAACGGCCGG
0361 GATGCGGATG ACGCCACTCA CNATTCCAGC GANAGCATCG AGGAGTGACG ATGGCAATGA
0421 AACCGATGAC GAGGAGGACG ACGACGAGTC GGAGGAGAGC AGCAGCGTCC AGACCGCGAA
0481 GGGAGTGCGC AAATACCACT TGGACGAC |
| in silico PCR Fragment |
0001 TGACAACCGG AAACGGAAGC GGCAACACGA TGACGTCGGC GTAC------ AAAATTCCAT
0061 CAAACAACAG CACAACAGCA AATGACAGCA GCAACACAGA GACAACATTC ACATTCAAAT
0121 TGGGCCGCTC GAACGGCCGA AGTAGCAGCA ATGTAGCCTC AAGCGAGTCA TCCGATCCAC
0181 TGGAGTCCGA TTACAGCGAG GAGGATCCGG AGC-AGGAGC AGCAGCGACC CGATCCGGCG
0241 ACCAACAGCC GGAGCAGCAG CACCGCCACC ACCACCACCA CCAGTTCCGC TGACCATGAC
0301 AATGACGTCG ACGAGGAGGA TGAGGAGGAC GACGAGAACG AGGGGGAGGG CAACGGCCGG
0361 GATGCGGATG ACGCCACTCA CGATTCCAGC GAGAGCATCG AGGAG-GACG ATGGCAATGA
0421 AACCGATGAC GAGGAGGACG ACGACGAGTC GGAGGAGAGC AGCAGCGTCC AGACCGCGAA
0481 GGGAGTGCGC AAATACCACT TGGACGAC |
| Assemble Data |
<PRE>
6117R-2.IR_full 1 TGACAACCGGAAACGGAAGCGGCAACACGATGACGTCGGCGTAC<span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span>AAAATTCCAT 60
||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||
6117R-2.in silico 1 TGACAACCGGAAACGGAAGCGGCAACACGATGACGTCGGCGTAC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>AAAATTCCAT 60
6117R-2.IR_full 61 CAAACAACAGCACAACAGCAAATGACAGCAGCAACACAGAGACAACATTCACATTCAAAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6117R-2.in silico 61 CAAACAACAGCACAACAGCAAATGACAGCAGCAACACAGAGACAACATTCACATTCAAAT 120
6117R-2.IR_full 121 TGGGCCGCTCGAACGGCCGAAGTAGCAGCAATGTAGCCTCAAGCGAGTCATCCGATCCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6117R-2.in silico 121 TGGGCCGCTCGAACGGCCGAAGTAGCAGCAATGTAGCCTCAAGCGAGTCATCCGATCCAC 180
6117R-2.IR_full 181 TGGAGTCCGATTACAGCGAGGAGGATCCGGAGC<span class="snp"><tt>G</tt></span>AGGAGCAGCAGCGACCCGATCCGGCG 240
|||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||
6117R-2.in silico 181 TGGAGTCCGATTACAGCGAGGAGGATCCGGAGC<span class="snp"><tt>-</tt></span>AGGAGCAGCAGCGACCCGATCCGGCG 240
6117R-2.IR_full 241 ACCAACAG<span class="snp"><tt>N</tt></span>CGGAGCAGCAGCACCGCCACCACCACCACCACCAGTTCCGCTGACCATGAC 300
||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
6117R-2.in silico 241 ACCAACAG<span class="snp"><tt>C</tt></span>CGGAGCAGCAGCACCGCCACCACCACCACCACCAGTTCCGCTGACCATGAC 300
6117R-2.IR_full 301 A<span class="snp"><tt>G</tt></span>TGACGTCGACGAGGAGGATGAGGAGGACGACGAGAACGAGGGGGAGGGCAACGGCCGG 360
|<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6117R-2.in silico 301 A<span class="snp"><tt>A</tt></span>TGACGTCGACGAGGAGGATGAGGAGGACGACGAGAACGAGGGGGAGGGCAACGGCCGG 360
6117R-2.IR_full 361 GATGCGGATGACGCCACTCAC<span class="snp"><tt>N</tt></span>ATTCCAGCGA<span class="snp"><tt>N</tt></span>AGCATCGAGGAG<span class="snp"><tt>T</tt></span>GACGATGGCAATGA 420
|||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||<span class="snp"><tt> </tt></span>||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||
6117R-2.in silico 361 GATGCGGATGACGCCACTCAC<span class="snp"><tt>G</tt></span>ATTCCAGCGA<span class="snp"><tt>G</tt></span>AGCATCGAGGAG<span class="snp"><tt>-</tt></span>GACGATGGCAATGA 420
6117R-2.IR_full 421 AACCGATGACGAGGAGGACGACGACGAGTCGGAGGAGAGCAGCAGCGTCCAGACCGCGAA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6117R-2.in silico 421 AACCGATGACGAGGAGGACGACGACGAGTCGGAGGAGAGCAGCAGCGTCCAGACCGCGAA 480
6117R-2.IR_full 481 GGGAGTGCGCAAATACCACTTGGACGAC 508
||||||||||||||||||||||||||||
6117R-2.in silico 481 GGGAGTGCGCAAATACCACTTGGACGAC 508
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
10 |
64 |
NM_168638.2 |
CG6117-RB, transcript variant B (Pka-C3), mRNA |
| 100 |
482 |
0 |
10 |
64 |
NM_079373.2 |
CG6117-RA, transcript variant A (Pka-C3), mRNA |
| 0.82 |
4 |
6 |
37 |
91 |
NM_001038940.1 |
CG34002-RA (CG34002), mRNA |
| 0.82 |
4 |
2 |
21 |
37 |
NM_132867.1 |
CG9170-RA, transcript variant A (CG9170), mRNA |
| 0.82 |
4 |
2 |
21 |
37 |
NM_206753.1 |
CG9170-RB, transcript variant B (CG9170), mRNA |
| 0.82 |
4 |
2 |
18 |
23 |
NM_078643.2 |
CG4211-RA, transcript variant A (nonA), mRNA |
| 0.82 |
4 |
2 |
18 |
23 |
NM_001014747.1 |
CG4211-RC, transcript variant C (nonA), mRNA |
| 0.82 |
4 |
2 |
18 |
23 |
NM_167505.1 |
CG4211-RB, transcript variant B (nonA), mRNA |
| 0.62 |
3 |
14 |
36 |
104 |
NM_001038742.1 |
CG6824-RD, transcript variant D (ovo), mRNA |
| 0.62 |
3 |
14 |
36 |
104 |
NM_080338.3 |
CG6824-RA, transcript variant A (ovo), mRNA |
| 0.62 |
3 |
14 |
36 |
94 |
NM_167027.2 |
CG6824-RC, transcript variant C (ovo), mRNA |
| 0.62 |
3 |
14 |
36 |
94 |
NM_167026.2 |
CG6824-RB, transcript variant B (ovo), mRNA |
| 0.62 |
3 |
9 |
54 |
90 |
NM_133104.2 |
CG7282-RA (CG7282), mRNA |
| 0.62 |
3 |
4 |
31 |
149 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 0.62 |
3 |
4 |
31 |
149 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 0.62 |
3 |
4 |
31 |
149 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 0.62 |
3 |
4 |
12 |
39 |
NM_001043262.1 |
CG34157-RE, transcript variant E (Dys), mRNA |
| 0.41 |
2 |
26 |
44 |
96 |
NM_143342.1 |
CG5514-RB, transcript variant B (CG5514), mRNA |
| 0.41 |
2 |
26 |
44 |
96 |
NM_170361.1 |
CG5514-RA, transcript variant A (CG5514), mRNA |
| 0.41 |
2 |
15 |
21 |
56 |
NM_143571.2 |
CG15544-RA (CG15544), mRNA |
| 0.41 |
2 |
12 |
7 |
23 |
NM_164749.1 |
CG5261-RA, transcript variant A (CG5261), mRNA |
| 0.41 |
2 |
12 |
7 |
23 |
NM_135274.2 |
CG5261-RB, transcript variant B (CG5261), mRNA |
| 0.41 |
2 |
10 |
11 |
18 |
NM_136711.2 |
CG11866-RA (CG11866), mRNA |
| 0.41 |
2 |
9 |
12 |
58 |
NM_168254.1 |
CG7915-RB, transcript variant B (Ect4), mRNA |
| 0.41 |
2 |
6 |
16 |
61 |
NM_132392.1 |
CG12641-RA (CG12641), mRNA |
| 0.41 |
2 |
6 |
15 |
28 |
NM_164597.1 |
CG15626-RB, transcript variant B (CG15626), mRNA |
| 0.41 |
2 |
6 |
15 |
28 |
NM_135020.2 |
CG15626-RA, transcript variant A (CG15626), mRNA |
| 0.41 |
2 |
6 |
12 |
30 |
NM_132386.2 |
CG2961-RA (Ipod), mRNA |
| 0.41 |
2 |
5 |
7 |
40 |
NM_165760.2 |
CG18408-RB, transcript variant B (CAP), mRNA |
| 0.41 |
2 |
4 |
37 |
64 |
NM_080319.3 |
CG4125-RA (rst), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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