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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6084R-1

Symbol

CG6084

Full Name

CG6084

CG No

CG6084

Old CG No

CG6084

Synonyms

CG6084, 34F4T, ESTS:34F4T

Accession No (Link to NCBI)

NM_140227.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Yang S, Zhao Y, Yu J, Fan Z, Gong ST, Tang H, Pan L.<br> Sugar Alcohols of Polyol Pathway Serve as Alarmins to Mediate Local-Systemic Innate Immune Communication in Drosophila.<br> Cell Host Microbe (2019) 26(2) 240-251.e8 [ PubMed ID = 31350199 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATCATTGGACTGGGAACCTG

Primer Seq. 3'

aatctagaggtaccTGGTTACTGGTGGAATAGTGG

Predicted Fragment Size

IR fragment full Seq

0001 AATCATTGGA CTGGGAACCT GGGGCAGCCC CAAgGGTCAG GTCACCGAGG CTGTCAAAGT 
0061 TGCCATTGAT GCCGGATACA GGCACATTGA CTGTGNCTAT GTATACCAAA ACGAGGATGA 
0121 GGTCGGAGAT GGTGTTGAGG CCAAGATCAA GGAGGGCGTT GTCAAGCGTG AGGATCTGTT 
0181 CATCACCAGC AAACTGTGGA ACACTTTCCA TCGCCCGGAT CTTGTCAAGT CGGCATTGGA 
0241 GAACACATTG AGCTCCCTGA AGCTGAAGTA CCTCGATCTG TACCTTATCC ACTGGNCCAT 
0301 GGGCTACAAG GAGGGATGCG ATCTGTTCCC CACCGACAAG GATGGCAAGA CGCTGTACTC 
0361 GCCGGTTGAT TACGTCGACA CGTGGAAGGC CATGGAGAAG TTGGTGGAAG AGGGTCTGGT 
0421 CAAGTCCATT GGTGTTTCCA ACTTCAACAG AAGGCAGATC GAGCGCGTGC TTGAGGTGGC 
0481 CACTATTCCA CCAGTAACCA

in silico PCR Fragment

0001 AATCATTGGA CTGGGAACCT GGGGCAGCCC CAAGGGTCAG GTCACCGAGG CTGTCAAAGT 
0061 TGCCATTGAT GCCGGATACA GGCACATTGA CTGTGCCTAT GTATACCAAA ACGAGGATGA 
0121 GGTCGGAGAT GGTGTTGAGG CCAAGATCAA GGAGGGCGTT GTCAAGCGTG AGGATCTGTT 
0181 CATCACCAGC AAACTGTGGA ACACTTTCCA TCGCCCGGAT CTTGTCAAGT CGGCATTGGA 
0241 GAACACATTG AGCTCCCTGA AGCTGAAGTA CCTCGATCTG TACCTTATCC ACTGGCCCAT 
0301 GGGCTACAAG GAGGGATGCG ATCTGTTCCC CACCGACAAG GATGGCAAGA CGCTGTACTC 
0361 GCCGGTTGAT TACGTCGACA CGTGGAAGGC CATGGAGAAG TTGGTGGAAG AGGGTCTGGT 
0421 CAAGTCCATT GGTGTTTCCA ACTTCAACAG AAGGCAGATC GAGCGCGTGC TTGAGGTGGC 
0481 CACTATTCCA CCAGTAACCA

Assemble Data

<PRE>

6084R-1.IR_full       1   AATCATTGGACTGGGAACCTGGGGCAGCCCCAAGGGTCAGGTCACCGAGGCTGTCAAAGT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6084R-1.in silico     1   AATCATTGGACTGGGAACCTGGGGCAGCCCCAAGGGTCAGGTCACCGAGGCTGTCAAAGT 60


6084R-1.IR_full       61  TGCCATTGATGCCGGATACAGGCACATTGACTGTG<span class="snp"><tt>N</tt></span>CTATGTATACCAAAACGAGGATGA 120
                          |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
6084R-1.in silico     61  TGCCATTGATGCCGGATACAGGCACATTGACTGTG<span class="snp"><tt>C</tt></span>CTATGTATACCAAAACGAGGATGA 120


6084R-1.IR_full       121 GGTCGGAGATGGTGTTGAGGCCAAGATCAAGGAGGGCGTTGTCAAGCGTGAGGATCTGTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6084R-1.in silico     121 GGTCGGAGATGGTGTTGAGGCCAAGATCAAGGAGGGCGTTGTCAAGCGTGAGGATCTGTT 180


6084R-1.IR_full       181 CATCACCAGCAAACTGTGGAACACTTTCCATCGCCCGGATCTTGTCAAGTCGGCATTGGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6084R-1.in silico     181 CATCACCAGCAAACTGTGGAACACTTTCCATCGCCCGGATCTTGTCAAGTCGGCATTGGA 240


6084R-1.IR_full       241 GAACACATTGAGCTCCCTGAAGCTGAAGTACCTCGATCTGTACCTTATCCACTGG<span class="snp"><tt>N</tt></span>CCAT 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
6084R-1.in silico     241 GAACACATTGAGCTCCCTGAAGCTGAAGTACCTCGATCTGTACCTTATCCACTGG<span class="snp"><tt>C</tt></span>CCAT 300


6084R-1.IR_full       301 GGGCTACAAGGAGGGATGCGATCTGTTCCCCACCGACAAGGATGGCAAGACGCTGTACTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6084R-1.in silico     301 GGGCTACAAGGAGGGATGCGATCTGTTCCCCACCGACAAGGATGGCAAGACGCTGTACTC 360


6084R-1.IR_full       361 GCCGGTTGATTACGTCGACACGTGGAAGGCCATGGAGAAGTTGGTGGAAGAGGGTCTGGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6084R-1.in silico     361 GCCGGTTGATTACGTCGACACGTGGAAGGCCATGGAGAAGTTGGTGGAAGAGGGTCTGGT 420


6084R-1.IR_full       421 CAAGTCCATTGGTGTTTCCAACTTCAACAGAAGGCAGATCGAGCGCGTGCTTGAGGTGGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6084R-1.in silico     421 CAAGTCCATTGGTGTTTCCAACTTCAACAGAAGGCAGATCGAGCGCGTGCTTGAGGTGGC 480


6084R-1.IR_full       481 CACTATTCCACCAGTAACCA 500
                          ||||||||||||||||||||
6084R-1.in silico     481 CACTATTCCACCAGTAACCA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	5	NM_140227.1	CG6084-RA, transcript variant A (CG6084), mRNA
94.81	457	1	1	6	NM_168467.1	CG6084-RB, transcript variant B (CG6084), mRNA
0.2	1	2	2	3	NM_169685.1	CG8884-RC, transcript variant C (Sap47), mRNA
0.2	1	2	2	3	NM_169690.1	CG8884-RB, transcript variant B (Sap47), mRNA
0.2	1	2	2	3	NM_079647.2	CG8884-RF, transcript variant F (Sap47), mRNA
0.2	1	2	2	3	NM_169689.1	CG8884-RG, transcript variant G (Sap47), mRNA
0.2	1	2	2	3	NM_169687.1	CG8884-RE, transcript variant E (Sap47), mRNA
0.2	1	2	2	3	NM_169686.1	CG8884-RD, transcript variant D (Sap47), mRNA
0.2	1	2	2	3	NM_169684.1	CG8884-RA, transcript variant A (Sap47), mRNA
0.2	1	2	2	3	NM_169688.1	CG8884-RH, transcript variant H (Sap47), mRNA
0	0	3	18	33	NM_140228.1	CG6083-RA (CG6083), mRNA
0	0	3	7	3	NM_170215.1	CG31288-RA (CG31288), mRNA
0	0	3	5	4	NM_167266.1	CG12101-RB, transcript variant B (Hsp60), mRNA
0	0	3	5	4	NM_078560.2	CG12101-RA, transcript variant A (Hsp60), mRNA
0	0	2	5	4	NM_143747.2	CG3762-RA, transcript variant A (Vha68-2), mRNA
0	0	2	5	4	NM_165021.1	CG3762-RB, transcript variant B (Vha68-2), mRNA
0	0	2	5	4	NM_165022.1	CG3762-RC, transcript variant C (Vha68-2), mRNA
0	0	2	5	3	NM_138154.2	CG32475-RA (mthl8), mRNA
0	0	2	5	2	NM_165180.1	CG11397-RA (glu), mRNA
0	0	2	3	14	NM_139582.1	CG12766-RA (CG12766), mRNA
0	0	1	7	5	NM_168199.1	CG8606-RB, transcript variant B (RhoGEF4), mRNA
0	0	1	7	5	NM_139844.2	CG8606-RA, transcript variant A (RhoGEF4), mRNA
0	0	1	5	8	NM_001014592.1	CG12306-RB, transcript variant B (polo), mRNA
0	0	1	5	8	NM_079455.3	CG12306-RA, transcript variant A (polo), mRNA
0	0	1	5	4	NM_133108.2	CG32542-RA (CG32542), mRNA
0	0	1	4	3	NM_135741.2	CG5776-RA (CG5776), mRNA
0	0	1	4	3	NM_079739.2	CG10161-RB (eIF-3p66), mRNA
0	0	1	3	3	NM_169434.1	CG6946-RB, transcript variant B (glo), mRNA
0	0	1	3	3	NM_141863.1	CG6946-RA, transcript variant A (glo), mRNA
0	0	1	3	3	NM_169435.1	CG6946-RC, transcript variant C (glo), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00040 : Pentose and glucuronate interconversions
dme00051 : Fructose and mannose metabolism
dme00052 : Galactose metabolism
dme00561 : Glycerolipid metabolism
dme00790 : Folate biosynthesis
dme01100 : Metabolic pathways

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