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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6049R-8 
 Symbol CG6049  Full Name CG6049 
 CG No CG6049  Old CG No CG6049 
 Synonyms Tat-SF1, cg6049, CG6049 
 Accession No (Link to NCBI) NM_141056.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAAGCGGAGGAAAAAAAGGGGG 
 Primer Seq. 3' 
aatctagaggtaccATCCTTGTCGGTGTAAGTGCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AAGCGGAGGA AAAAAAGGGG GACGCAGAAG GACAGGAAGC GAAAGCTCCA ATTCTCAACC 
0061 CCATTTCAGT CCCAGAAGTT GATGACAAGC CGACGGAAAA TAAACCCCAA TCGGATAATC 
0121 ACGCGGACAA AACGGACGAA ACTCCATCTC AGGACTTTGC CGCCTACGAG GAGCACATGA 
0181 CCTATGGAGC GGACGGCGGG GCTTATATAC ACGGATCCCA GCACGAAGCA GAAATACAAG 
0241 TGGTGCGCCA CCGGAAACAA TTGGCAACCC TTAGGCGTTG ATGAAGATGT TGATGGACAG 
0301 GCTGAAGATC CCTACGAAAA CGAGCACTAC AAGTGGTGTC CCAAATCCCA ACAGTGGTTG 
0361 CCAAAAAAAC AGGAAACTGA AACGGAGCAC TACAAGTGGG ATGACGAGCA AAAGAANGTG 
0421 GGTGCCCAAG CACCCGANAC CCTGGCCAGG AAGGGGTTTG CGGTGTGGAT GAGCATGGCG 
0481 ANGCGCACTT ACACCGACAA GGAT 
 in silico PCR Fragment 
0001 AAGCGGAGGA AAAAAAGGGG GACGCAGAAG GACAGGAAGC GAAAGCTCCA ATTCTCAACC 
0061 CCATTTCAGT CCCAGAAGTT GATGACAAGC CGACGGAAAA TAAACCCCAA TCGGATAATC 
0121 ACGCGGACAA AACGGACGAA ACTCCATCTC AGGACTTTGC CGCCTACGAG GAGCACATGA 
0181 CCTATGGAGC GGACGGCGGG GC-TATATAC ACGGATCCCA GCACGAAGCA GAAATACAAG 
0241 TGGTGCGCCA CCGGAAACAA TTGGCAACCC TTAGGCGTTG ATGAAGATGT TGATGGACAG 
0301 GCTGAAGATC CCTACGAAAA CGAGCACTAC AAGTGGTGTC CCAAATCCCA ACAGTGGTTG 
0361 CCAAAAAAAC AGGAAACTGA AACGGAGCAC TACAAGTGGG ATGACGAGCA AAAGAA-GTG 
0421 GGTGCCCAAG CACCCGA-AC CCTGGCCAGG AAGGGGTTTG CGGTGTGGAT GAGCATGGCG 
0481 A-GCGCACTT ACACCGACAA GGAT 
 Assemble Data 
<PRE>

6049R-8.IR_full       1   AAGCGGAGGAAAAAAAGGGGGACGCAGAAGGACAGGAAGCGAAAGCTCCAATTCTCAACC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6049R-8.in silico     1   AAGCGGAGGAAAAAAAGGGGGACGCAGAAGGACAGGAAGCGAAAGCTCCAATTCTCAACC 60


6049R-8.IR_full       61  CCATTTCAGTCCCAGAAGTTGATGACAAGCCGACGGAAAATAAACCCCAATCGGATAATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6049R-8.in silico     61  CCATTTCAGTCCCAGAAGTTGATGACAAGCCGACGGAAAATAAACCCCAATCGGATAATC 120


6049R-8.IR_full       121 ACGCGGACAAAACGGACGAAACTCCATCTCAGGACTTTGCCGCCTACGAGGAGCACATGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6049R-8.in silico     121 ACGCGGACAAAACGGACGAAACTCCATCTCAGGACTTTGCCGCCTACGAGGAGCACATGA 180


6049R-8.IR_full       181 CCTATGGAGCGGACGGCGGGGC<span class="snp"><tt>T</tt></span>TATATACACGGATCCCAGCACGAAGCAGAAATACAAG 240
                          ||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
6049R-8.in silico     181 CCTATGGAGCGGACGGCGGGGC<span class="snp"><tt>-</tt></span>TATATACACGGATCCCAGCACGAAGCAGAAATACAAG 240


6049R-8.IR_full       241 TGGTGCGCCACCGGAAACAATTGGCAACCCTTAGGCGTTGATGAAGATGTTGATGGACAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6049R-8.in silico     241 TGGTGCGCCACCGGAAACAATTGGCAACCCTTAGGCGTTGATGAAGATGTTGATGGACAG 300


6049R-8.IR_full       301 GCTGAAGATCCCTACGAAAACGAGCACTACAAGTGGTGTCCCAAATCCCAACAGTGGTTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6049R-8.in silico     301 GCTGAAGATCCCTACGAAAACGAGCACTACAAGTGGTGTCCCAAATCCCAACAGTGGTTG 360


6049R-8.IR_full       361 CCAAAAAAACAGGAAACTGAAACGGAGCACTACAAGTGGGATGACGAGCAAAAGAA<span class="snp"><tt>N</tt></span>GTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
6049R-8.in silico     361 CCAAAAAAACAGGAAACTGAAACGGAGCACTACAAGTGGGATGACGAGCAAAAGAA<span class="snp"><tt>-</tt></span>GTG 420


6049R-8.IR_full       421 GGTGCCCAAGCACCCGA<span class="snp"><tt>N</tt></span>ACCCTGGCCAGGAAGGGGTTTGCGGTGTGGATGAGCATGGCG 480
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
6049R-8.in silico     421 GGTGCCCAAGCACCCGA<span class="snp"><tt>-</tt></span>ACCCTGGCCAGGAAGGGGTTTGCGGTGTGGATGAGCATGGCG 480


6049R-8.IR_full       481 A<span class="snp"><tt>N</tt></span>GCGCACTTACACCGACAAGGAT 504
                          |<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
6049R-8.in silico     481 A<span class="snp"><tt>-</tt></span>GCGCACTTACACCGACAAGGAT 504

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  6  8  NM_168901.2  CG6049-RB, transcript variant B (CG6049), mRNA 
100   482  0  6  8  NM_141056.3  CG6049-RA, transcript variant A (CG6049), mRNA 
100   482  0  6  8  NM_168902.2  CG6049-RC, transcript variant C (CG6049), mRNA 
0   0  3  4  2  NM_137810.1  CG3290-RA (CG3290), mRNA 
0   0  3  3  4  NM_167448.1  CG6340-RA, transcript variant A (CG6340), mRNA 
0   0  2  6  3  NM_135386.3  CG13401-RA, transcript variant A (U26), mRNA 
0   0  2  6  3  NM_205938.1  CG13401-RB, transcript variant B (U26), mRNA 
0   0  2  3  2  NM_079217.2  CG10539-RA (S6k), mRNA 
0   0  2  2  6  NM_057608.4  CG17603-RA, transcript variant A (Taf1), mRNA 
0   0  2  2  6  NM_206437.1  CG17603-RC, transcript variant C (Taf1), mRNA 
0   0  2  2  6  NM_206438.1  CG17603-RB, transcript variant B (Taf1), mRNA 
0   0  1  3  14  NM_205901.1  CG3304-RC, transcript variant C (CG3304), mRNA 
0   0  1  3  11  NM_134926.2  CG3304-RA, transcript variant A (CG3304), mRNA 
0   0  1  3  9  NM_205902.1  CG3304-RB, transcript variant B (CG3304), mRNA 
0   0  1  3  3  NM_164525.1  CG9660-RC, transcript variant C (toc), mRNA 
0   0  1  3  3  NM_001042864.1  CG9660-RE, transcript variant E (toc), mRNA 
0   0  1  3  3  NM_164526.1  CG9660-RB, transcript variant B (toc), mRNA 
0   0  1  3  3  NM_164524.1  CG9660-RD, transcript variant D (toc), mRNA 
0   0  1  3  3  NM_057805.2  CG9660-RA, transcript variant A (toc), mRNA 
0   0  1  3  3  NM_135942.2  CG17328-RA (CG17328), mRNA 
0   0  1  3  2  NM_139741.1  CG10486-RA (CG10486), mRNA 
0   0  1  3  2  NM_139762.1  CG6600-RA (CG6600), mRNA 
0   0  1  2  2  NM_170054.2  CG17894-RB, transcript variant B (cnc), mRNA 
0   0  1  2  2  NM_170053.2  CG17894-RC, transcript variant C (cnc), mRNA 
0   0  0  7  5  NM_141950.2  CG12267-RA (CG12267), mRNA 
0   0  0  6  4  NM_168801.1  CG9279-RB, transcript variant B (CG9279), mRNA 
0   0  0  6  4  NM_140867.1  CG9279-RA, transcript variant A (CG9279), mRNA 
0   0  0  6  2  NM_057956.2  CG10269-RA (D19A), mRNA 
0   0  0  5  2  NM_143309.1  CG13972-RA (CG13972), mRNA 
0   0  0  4  10  NM_132097.1  CG3950-RA (CG3950), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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