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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

5998R-2

Symbol

Adgf-B

Full Name

Adenosine deaminase-related growth factor B

CG No

CG5998

Old CG No

CG5998

Synonyms

CG5998, ADGF-B, adgfb, Adgf-B

Accession No (Link to NCBI)

NM_140749.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGTCGCCGAGCATTGACCAAA

Primer Seq. 3'

aatctagaggtaccGGCTCCCAGATAGATTCCTTA

Predicted Fragment Size

IR fragment full Seq

0001 TGTCGCCGAG CATTGACCAA ATGTGCAAAA ATTGGCCACA CTACAGTGTC CGTGAGTCGG 
0061 TCCCTCCGGA AACTGTCACC CACGGCGTTC AAGACACTTC GACAAGCAAT CGCCAAGGTG 
0121 GGGCACTTAA ACATCATGGG TCAGAAGATA CGACTGAACG ATCGGGAATT AGCGGCCAAC 
0181 AAAGTGATCA TGGCCGTCAA GAAGGAAGAG ATATCCAAGG GCATACTGGA TCCCAGTCAG 
0241 TTCACGCCGG GCCAGCACAT CTTTAAGACC CTGGCCGAGG TGCAAAAGTC GCCGATCTTT 
0301 AACCTGATTA AGGGTATGCC GAAGGGCGGC GCGCTTCATG CCCACGATAC GGCGCTCTGC 
0361 AGCACAGACT ACCTCATAAG TCTGACCTAC CGGAAGAATC TTTGGATTTG TACCGCTGAG 
0421 GAGGGCTGCA AGGCCATTGC TTTCCGGTTT TCCAAGGAGG AGCCCAAGGA AAAACCTGTT 
0481 AAGGAATCTA TCTGGGAGCC

in silico PCR Fragment

0001 TGTCGCCGAG CATTGACCAA ATGTGCAAAA ATTGGCCACA CTACAGTGTC CGTGAGTCGG 
0061 TCCCTCCGGA AACTGTCACC CACGGCGTTC AAGACACTTC GACAAGCAAT CGCCAAGGTG 
0121 GGGCACTTAA ACATCATGGG TCAGAAGATA CGACTGAACG ATCGGGAATT AGCGGCCAAC 
0181 AAAGTGATCA TGGCCGTCAA GAAGGAAGAG ATATCCAAGG GCATACTGGA TCCCAGTCAG 
0241 TTCACGCCGG GCCAGCACAT CTTTAAGACC CTGGCCGAGG TGCAAAAGTC GCCGATCTTT 
0301 AACCTGATTA AGGGTATGCC GAAGGGCGGC GCGCTTCATG CCCACGATAC GGCGCTCTGC 
0361 AGCACAGACT ACCTCATAAG TCTGACCTAC CGGAAGAATC TTTGGATTTG TACCGCTGAG 
0421 GAGGGCTGCA AGGCCATTGC TTTCCGGTTT TCCAAGGAGG AGCCCAAGGA AAAACCTGTT 
0481 AAGGAATCTA TCTGGGAGCC

Assemble Data

<PRE>

5998R-2.IR_full       1   TGTCGCCGAGCATTGACCAAATGTGCAAAAATTGGCCACACTACAGTGTCCGTGAGTCGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     1   TGTCGCCGAGCATTGACCAAATGTGCAAAAATTGGCCACACTACAGTGTCCGTGAGTCGG 60


5998R-2.IR_full       61  TCCCTCCGGAAACTGTCACCCACGGCGTTCAAGACACTTCGACAAGCAATCGCCAAGGTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     61  TCCCTCCGGAAACTGTCACCCACGGCGTTCAAGACACTTCGACAAGCAATCGCCAAGGTG 120


5998R-2.IR_full       121 GGGCACTTAAACATCATGGGTCAGAAGATACGACTGAACGATCGGGAATTAGCGGCCAAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     121 GGGCACTTAAACATCATGGGTCAGAAGATACGACTGAACGATCGGGAATTAGCGGCCAAC 180


5998R-2.IR_full       181 AAAGTGATCATGGCCGTCAAGAAGGAAGAGATATCCAAGGGCATACTGGATCCCAGTCAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     181 AAAGTGATCATGGCCGTCAAGAAGGAAGAGATATCCAAGGGCATACTGGATCCCAGTCAG 240


5998R-2.IR_full       241 TTCACGCCGGGCCAGCACATCTTTAAGACCCTGGCCGAGGTGCAAAAGTCGCCGATCTTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     241 TTCACGCCGGGCCAGCACATCTTTAAGACCCTGGCCGAGGTGCAAAAGTCGCCGATCTTT 300


5998R-2.IR_full       301 AACCTGATTAAGGGTATGCCGAAGGGCGGCGCGCTTCATGCCCACGATACGGCGCTCTGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     301 AACCTGATTAAGGGTATGCCGAAGGGCGGCGCGCTTCATGCCCACGATACGGCGCTCTGC 360


5998R-2.IR_full       361 AGCACAGACTACCTCATAAGTCTGACCTACCGGAAGAATCTTTGGATTTGTACCGCTGAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     361 AGCACAGACTACCTCATAAGTCTGACCTACCGGAAGAATCTTTGGATTTGTACCGCTGAG 420


5998R-2.IR_full       421 GAGGGCTGCAAGGCCATTGCTTTCCGGTTTTCCAAGGAGGAGCCCAAGGAAAAACCTGTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5998R-2.in silico     421 GAGGGCTGCAAGGCCATTGCTTTCCGGTTTTCCAAGGAGGAGCCCAAGGAAAAACCTGTT 480


5998R-2.IR_full       481 AAGGAATCTATCTGGGAGCC 500
                          ||||||||||||||||||||
5998R-2.in silico     481 AAGGAATCTATCTGGGAGCC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140749.2	CG5998-RA (Adgf-B), mRNA
3.31	16	2	4	5	NM_206397.1	CG5992-RB, transcript variant B (Adgf-A), mRNA
3.31	16	2	4	5	NM_079406.2	CG5992-RA, transcript variant A (Adgf-A), mRNA
0.62	3	4	2	2	NM_136515.2	CG8709-RA (CG8709), mRNA
0	0	4	2	2	NM_079270.1	CG4190-RA (Hsp67Bc), mRNA
0	0	2	3	2	NM_137155.2	CG10228-RA (Pcf11), mRNA
0	0	1	6	8	NM_169562.1	CG3028-RA (Ipp), mRNA
0	0	1	3	16	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	1	3	6	NM_137195.2	CG8180-RA (CG8180), mRNA
0	0	1	3	5	NM_166141.2	CG8421-RD, transcript variant D (Asph), mRNA
0	0	1	3	5	NM_079033.2	CG8421-RB, transcript variant B (Asph), mRNA
0	0	1	3	4	NM_139419.1	CG13925-RA (CG13925), mRNA
0	0	1	3	3	NM_142180.2	CG4285-RA (CG4285), mRNA
0	0	1	3	2	NM_058157.3	CG2835-RB, transcript variant B (G-salpha60A), mRNA
0	0	1	3	2	NM_176185.1	CG8421-RE, transcript variant E (Asph), mRNA
0	0	1	3	2	NM_166140.1	CG8421-RA, transcript variant A (Asph), mRNA
0	0	1	3	2	NM_058158.3	CG2835-RA, transcript variant A (G-salpha60A), mRNA
0	0	1	3	2	NM_137119.2	CG10105-RA (CG10105), mRNA
0	0	1	3	2	NM_166640.1	CG2835-RC, transcript variant C (G-salpha60A), mRNA
0	0	1	2	12	NM_001032053.1	CG33715-RB, transcript variant B (Msp-300), mRNA
0	0	0	6	5	NM_166907.1	CG14801-RC, transcript variant C (CG14801), mRNA
0	0	0	6	4	NM_130589.1	CG14801-RB, transcript variant B (CG14801), mRNA
0	0	0	6	4	NM_166906.1	CG14801-RA, transcript variant A (CG14801), mRNA
0	0	0	6	4	NM_166908.1	CG14801-RD, transcript variant D (CG14801), mRNA
0	0	0	6	3	NM_079705.2	CG5760-RA (rtet), mRNA
0	0	0	5	3	NM_164958.1	CG31867-RA (CG31867), mRNA
0	0	0	4	2	NM_206693.1	CG1522-RF, transcript variant F (cac), mRNA
0	0	0	4	2	NM_001014732.1	CG1522-RJ, transcript variant J (cac), mRNA
0	0	0	4	2	NM_001014735.1	CG1522-RG, transcript variant G (cac), mRNA
0	0	0	4	2	NM_135360.3	CG7830-RA (CG7830), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00230 : Purine metabolism
dme01100 : Metabolic pathways
dme01232 : Nucleotide metabolism

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