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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5955R-1 |
| Symbol |
CG5955 |
Full Name |
CG5955 |
| CG No |
CG5955 |
Old CG No |
CG5955 |
| Synonyms |
CG5955 |
| Accession No (Link to NCBI) |
NM_140973.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTAGGCCACCCAAAATTCTGA |
| Primer Seq. 3' |
aatctagaggtaccGCATCTGAAGTCCAGTCCAAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GNAGGCCACC CAAAATTCTG ATCACAGGTG GCTTGGGcCA GTTGGGCATC GAGTGCGCCA
0061 AACTCCTGCG GACGCAGTAT GGCAGCCAAA ACGTGATCCT CTCGGACATT ATAAAGCCCA
0121 GTCAGTCGGT ACTGGAGAAT GGTCCCTACA TTTTTGCCGA CATCCTGGAC TTCAAGGGTC
0181 TCCAGAAGAT CGTTGTGGAC CATCGCATTG ACTGGCTGAT CCACTTCTCG GCCCTGCTGA
0241 GTGCGGTGGG AGAGCAAAAT GTTCCACTGG CTGTGAGGGT CAACATCGAG GGTGTCCACA
0301 ACGTCATCNA gCTgGCGAAG CAGTATAAgN TNCGCATCTT CGTACCAAGC ACAATCGGAG
0361 cCTTCGGTCC CGACAGTCCT CGCAATCCCA CGCCGAATGT TACGATCCAA CGGCCGCGCA
0421 CCATTTACGG CGTTTCCAAG GTGCACGCCG AGCTGATTGG CGAATACTAC TACCACAAGT
0481 TTGGACTGGA CTTCANGANG c |
| in silico PCR Fragment |
0001 GTAGGCCACC CAAAATTCTG ATCACAGGTG GCTTGGGCCA GTTGGGCATC GAGTGCGCCA
0061 AACTCCTGCG GACGCAGTAT GGCAGCCAAA ACGTGATCCT CTCGGACATT ATAAAGCCCA
0121 GTCAGTCGGT ACTGGAGAAT GGTCCCTACA TTTTTGCCGA CATCCTGGAC TTCAAGGGTC
0181 TCCAGAAGAT CGTTGTGGAC CATCGCATTG ACTGGCTGAT CCATTTCTCG GCCCTGCTGA
0241 GTGCGGTGGG AGAGCAAAAT GTTCCACTGG CTGTGAGGGT CAACATCGAG GGTGTCCACA
0301 ACGTCATCGA GCTGGCGAAG CAGTATAAGC TCCGCATCTT CGTACCAAGC ACAATCGGAG
0361 CCTTCGGTCC CGACAGTCCT CGCAATCCCA CGCCGAATGT TACGATCCAA CGGCCGCGCA
0421 CCATTTACGG CGTTTCCAAG GTGCACGCCG AGCTGATTGG CGAATACTAC TACCACAAGT
0481 TTGGACTGGA CTTCA-GATG C |
| Assemble Data |
<PRE>
5955R-1.IR_full 1 G<span class="snp"><tt>N</tt></span>AGGCCACCCAAAATTCTGATCACAGGTGGCTTGGGCCAGTTGGGCATCGAGTGCGCCA 60
|<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5955R-1.in silico 1 G<span class="snp"><tt>T</tt></span>AGGCCACCCAAAATTCTGATCACAGGTGGCTTGGGCCAGTTGGGCATCGAGTGCGCCA 60
5955R-1.IR_full 61 AACTCCTGCGGACGCAGTATGGCAGCCAAAACGTGATCCTCTCGGACATTATAAAGCCCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5955R-1.in silico 61 AACTCCTGCGGACGCAGTATGGCAGCCAAAACGTGATCCTCTCGGACATTATAAAGCCCA 120
5955R-1.IR_full 121 GTCAGTCGGTACTGGAGAATGGTCCCTACATTTTTGCCGACATCCTGGACTTCAAGGGTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5955R-1.in silico 121 GTCAGTCGGTACTGGAGAATGGTCCCTACATTTTTGCCGACATCCTGGACTTCAAGGGTC 180
5955R-1.IR_full 181 TCCAGAAGATCGTTGTGGACCATCGCATTGACTGGCTGATCCA<span class="snp"><tt>C</tt></span>TTCTCGGCCCTGCTGA 240
|||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||
5955R-1.in silico 181 TCCAGAAGATCGTTGTGGACCATCGCATTGACTGGCTGATCCA<span class="snp"><tt>T</tt></span>TTCTCGGCCCTGCTGA 240
5955R-1.IR_full 241 GTGCGGTGGGAGAGCAAAATGTTCCACTGGCTGTGAGGGTCAACATCGAGGGTGTCCACA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5955R-1.in silico 241 GTGCGGTGGGAGAGCAAAATGTTCCACTGGCTGTGAGGGTCAACATCGAGGGTGTCCACA 300
5955R-1.IR_full 301 ACGTCATC<span class="snp"><tt>N</tt></span>AGCTGGCGAAGCAGTATAAG<span class="snp"><tt>N</tt></span>T<span class="snp"><tt>N</tt></span>CGCATCTTCGTACCAAGCACAATCGGAG 360
||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||
5955R-1.in silico 301 ACGTCATC<span class="snp"><tt>G</tt></span>AGCTGGCGAAGCAGTATAAG<span class="snp"><tt>C</tt></span>T<span class="snp"><tt>C</tt></span>CGCATCTTCGTACCAAGCACAATCGGAG 360
5955R-1.IR_full 361 CCTTCGGTCCCGACAGTCCTCGCAATCCCACGCCGAATGTTACGATCCAACGGCCGCGCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5955R-1.in silico 361 CCTTCGGTCCCGACAGTCCTCGCAATCCCACGCCGAATGTTACGATCCAACGGCCGCGCA 420
5955R-1.IR_full 421 CCATTTACGGCGTTTCCAAGGTGCACGCCGAGCTGATTGGCGAATACTACTACCACAAGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5955R-1.in silico 421 CCATTTACGGCGTTTCCAAGGTGCACGCCGAGCTGATTGGCGAATACTACTACCACAAGT 480
5955R-1.IR_full 481 TTGGACTGGACTTCA<span class="snp"><tt>N</tt></span>GA<span class="snp"><tt>N</tt></span>GC 501
|||||||||||||||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>||
5955R-1.in silico 481 TTGGACTGGACTTCA<span class="snp"><tt>-</tt></span>GA<span class="snp"><tt>T</tt></span>GC 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140973.1 |
CG5955-RA (CG5955), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_142070.2 |
CG3050-RA, transcript variant A (Cyp6d5), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169567.1 |
CG3050-RB, transcript variant B (Cyp6d5), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137358.2 |
CG12699-RA (CG12699), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_168275.1 |
CG32354-RA (CG32354), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_206391.1 |
CG33256-RA (CG33256), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_142538.1 |
CG5237-RA (CG5237), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_135019.1 |
CG15627-RA (CG15627), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_078493.2 |
CG4206-RA (Mcm3), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_140464.1 |
CG17173-RA (CG17173), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_164895.1 |
CG4912-RA, transcript variant A (eEF1delta), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_078706.2 |
CG1403-RA, transcript variant A (Sep1), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167747.1 |
CG1403-RB, transcript variant B (Sep1), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_137785.1 |
CG9308-RA (CG9308), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_132125.1 |
CG4536-RA (CG4536), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_134864.3 |
CG2848-RA (Trn-SR), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_138033.1 |
CG13563-RA (CG13563), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_138091.2 |
CG3511-RA (CG3511), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_143497.2 |
CG7896-RA (CG7896), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_168094.1 |
CG32243-RA (CG32243), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_143790.1 |
CG11538-RA (CG11538), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_080077.2 |
CG6806-RA (Lsp2), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168268.1 |
CG7176-RE, transcript variant E (Idh), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168266.1 |
CG7176-RD, transcript variant D (Idh), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_143787.2 |
CG7176-RC, transcript variant C (Idh), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168265.1 |
CG7176-RB, transcript variant B (Idh), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_140769.1 |
CG5290-RA (CG5290), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_176298.1 |
CG7176-RG, transcript variant G (Idh), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168269.1 |
CG7176-RF, transcript variant F (Idh), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_168267.1 |
CG7176-RA, transcript variant A (Idh), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00260
:
Glycine, serine and threonine metabolism
dme01100
:
Metabolic pathways
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