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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5932R-1 
 Symbol CG5932  Full Name CG5932 
 CG No CG5932  Old CG No CG5932 
 Synonyms CG5932 
 Accession No (Link to NCBI) NM_140972.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATACGATGTGTGGCTGGGCA 
 Primer Seq. 3' 
aatctagaggtaccGGAGCTCGCATTGGCATTAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GATACGATGT GTGGCTGGgC AATGCCCGTG GTAACATCTA CTCGCGGAAC AACATCATCA 
0061 TCTCGCTGAA CAGCCACAAG TTCTGGCACT TCGACTGGCA CGAAATCGGC ACCATCGACA 
0121 TCCCCGCCAT GATCGACTAN CATCCTGGCG GATACCGGCT TCGATCAGAT ACACTACGCC 
0181 GGACACTCGC AAGGAACCAC CGTGTACCTG GTGATGCTTT CCGAGCGACC GGAGTACAAT 
0241 GCCCTCATTA AGTCCGGTCA TTTGCTGGCT CCATGTGCCT TCTTCGAGCA CGGAACCTCC 
0301 TTCATCTTCA ATGCCCTGGG TCCCCTGGTG GGTACACCCG GCGGCATCTG GAACCAACTG 
0361 CTGGTGGACA CCGAACTGAT TCCGCACAAC AACCTGGTTA ACCGATTGGT CGACAACAGT 
0421 TGCCATCTAT CCAACACGAT TTGCAACAAC GCCTTCATAA TGTTTGCCAA TGGTGGATAC 
0481 GTTAATGCCA NTGNGAGCTC C 
 in silico PCR Fragment 
0001 GATACGATGT GTGGCTGGGC AATGCCCGTG GTAACATCTA CTCGCGGAAC AACATCATCA 
0061 TCTCGCTGAA CAGCCACAAG TTCTGGCACT TCGACTGGCA CGAAATCGGC ACCATCGACA 
0121 TCCCCGCCAT GATCGACTA- CATCCTGGCG GATACCGGCT TCGATCAGAT ACACTACGCC 
0181 GGACACTCGC AAGGAACCAC CGTGTACCTG GTGATGCTTT CCGAGCGACC GGAGTACAAT 
0241 GCCCTCATTA AGTCCGGTCA TTTGCTGGCT CCATGTGCCT TCTTCGAGCA CGGAACCTCC 
0301 TTCATCTTCA ATGCCCTGGG TCCCCTGGTG GGTACACCCG GCGGCATCTG GAACCAACTG 
0361 CTGGTGGACA CCGAACTGAT TCCGAACAAC AACCTGGTTA ACCGATTGGT CGACAACAGT 
0421 TGCCATCTAT CCAACACGAT TTGCAACAAC GCCTTCATAA TGTTTGCCAA TGGTGGATAC 
0481 GTTAATGCCA ATGCGAGCTC C 
 Assemble Data 
<PRE>

5932R-1.IR_full       1   GATACGATGTGTGGCTGGGCAATGCCCGTGGTAACATCTACTCGCGGAACAACATCATCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5932R-1.in silico     1   GATACGATGTGTGGCTGGGCAATGCCCGTGGTAACATCTACTCGCGGAACAACATCATCA 60


5932R-1.IR_full       61  TCTCGCTGAACAGCCACAAGTTCTGGCACTTCGACTGGCACGAAATCGGCACCATCGACA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5932R-1.in silico     61  TCTCGCTGAACAGCCACAAGTTCTGGCACTTCGACTGGCACGAAATCGGCACCATCGACA 120


5932R-1.IR_full       121 TCCCCGCCATGATCGACTA<span class="snp"><tt>N</tt></span>CATCCTGGCGGATACCGGCTTCGATCAGATACACTACGCC 180
                          |||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||
5932R-1.in silico     121 TCCCCGCCATGATCGACTA<span class="snp"><tt>-</tt></span>CATCCTGGCGGATACCGGCTTCGATCAGATACACTACGCC 180


5932R-1.IR_full       181 GGACACTCGCAAGGAACCACCGTGTACCTGGTGATGCTTTCCGAGCGACCGGAGTACAAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5932R-1.in silico     181 GGACACTCGCAAGGAACCACCGTGTACCTGGTGATGCTTTCCGAGCGACCGGAGTACAAT 240


5932R-1.IR_full       241 GCCCTCATTAAGTCCGGTCATTTGCTGGCTCCATGTGCCTTCTTCGAGCACGGAACCTCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5932R-1.in silico     241 GCCCTCATTAAGTCCGGTCATTTGCTGGCTCCATGTGCCTTCTTCGAGCACGGAACCTCC 300


5932R-1.IR_full       301 TTCATCTTCAATGCCCTGGGTCCCCTGGTGGGTACACCCGGCGGCATCTGGAACCAACTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5932R-1.in silico     301 TTCATCTTCAATGCCCTGGGTCCCCTGGTGGGTACACCCGGCGGCATCTGGAACCAACTG 360


5932R-1.IR_full       361 CTGGTGGACACCGAACTGATTCCG<span class="snp"><tt>C</tt></span>ACAACAACCTGGTTAACCGATTGGTCGACAACAGT 420
                          ||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
5932R-1.in silico     361 CTGGTGGACACCGAACTGATTCCG<span class="snp"><tt>A</tt></span>ACAACAACCTGGTTAACCGATTGGTCGACAACAGT 420


5932R-1.IR_full       421 TGCCATCTATCCAACACGATTTGCAACAACGCCTTCATAATGTTTGCCAATGGTGGATAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5932R-1.in silico     421 TGCCATCTATCCAACACGATTTGCAACAACGCCTTCATAATGTTTGCCAATGGTGGATAC 480


5932R-1.IR_full       481 GTTAATGCCA<span class="snp"><tt>N</tt></span>TG<span class="snp"><tt>N</tt></span>GAGCTCC 501
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>|||||||
5932R-1.in silico     481 GTTAATGCCA<span class="snp"><tt>A</tt></span>TG<span class="snp"><tt>C</tt></span>GAGCTCC 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140972.2  CG5932-RA (CG5932), mRNA 
1.24   6  4  7  5  NM_141960.1  CG11600-RA (CG11600), mRNA 
0.2   1  3  1  17  NM_141962.1  CG6753-RA (CG6753), mRNA 
0   0  9  11  5  NM_170248.1  CG31091-RA (CG31091), mRNA 
0   0  8  9  22  NM_057983.2  CG8823-RA (Lip3), mRNA 
0   0  7  11  5  NM_136294.3  CG3635-RA (CG3635), mRNA 
0   0  3  3  2  NM_058041.2  CG12467-RA (CG12467), mRNA 
0   0  3  2  2  NM_142989.3  CG6413-RA (Dis3), mRNA 
0   0  2  8  9  NM_078816.2  CG7279-RA (Lip1), mRNA 
0   0  2  3  3  NM_176350.1  CG14085-RB, transcript variant B (CG14085), mRNA 
0   0  2  3  3  NM_140844.1  CG14085-RA, transcript variant A (CG14085), mRNA 
0   0  2  2  2  NM_142531.1  CG14282-RA (CG14282), mRNA 
0   0  1  7  15  NM_001014548.1  CG11406-RB, transcript variant B (CG11406), mRNA 
0   0  1  7  15  NM_138053.2  CG11406-RA, transcript variant A (CG11406), mRNA 
0   0  1  5  2  NM_168485.1  CG5684-RC, transcript variant C (Pop2), mRNA 
0   0  1  5  2  NM_168484.1  CG5684-RB, transcript variant B (Pop2), mRNA 
0   0  1  5  2  NM_140281.2  CG5684-RA, transcript variant A (Pop2), mRNA 
0   0  1  3  6  NM_166896.1  CG11491-RB, transcript variant B (br), mRNA 
0   0  1  3  6  NM_166897.1  CG11491-RC, transcript variant C (br), mRNA 
0   0  1  3  3  NM_141413.1  CG15184-RA (CG15184), mRNA 
0   0  1  3  2  NM_079950.2  CG9540-RA (Ag5r2), mRNA 
0   0  1  2  7  NM_167164.2  CG12737-RB, transcript variant B (Crag), mRNA 
0   0  1  2  7  NM_080341.2  CG12737-RA, transcript variant A (Crag), mRNA 
0   0  1  2  3  NM_057880.3  CG15441-RA, transcript variant A (Gs1l), mRNA 
0   0  1  2  2  NM_138163.1  CG7036-RA, transcript variant A (rno), mRNA 
0   0  1  2  2  NM_206222.1  CG7036-RB, transcript variant B (rno), mRNA 
0   0  0  28  40  NM_134932.2  CG2772-RA (CG2772), mRNA 
0   0  0  9  11  NM_137176.1  CG8093-RA (CG8093), mRNA 
0   0  0  8  4  NM_142670.2  CG5621-RA, transcript variant A (CG5621), mRNA 
0   0  0  6  10  NM_176104.1  CG33087-RC (CG33087), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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