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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

5876R-2

Symbol

heix

Full Name

heixuedian

CG No

CG5876

Old CG No

CG5876

Synonyms

l(2)35Fc, ipa-6d, BG:DS02740.7, 124/1, l(2)k12401, CG5876, heix

Accession No (Link to NCBI)

NM_078857.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTCCAGGAATGGCAAGACCAA

Primer Seq. 3'

aatctagaggtaccTCAGTGCCAGGTGCTCCATTT

Predicted Fragment Size

IR fragment full Seq

0001 GTCCAGGAAT GGCAAGACCA AGACAGAGGA CGGTGAGGAG GTGGAAGCCG TTGTTGGAGC 
0061 TCGTGCAGCA GGTGCAGATG CCGGAGTAGC ACTAACGGGA CGACTAACAG GACACCCATC 
0121 CACATCCGGA ACGTTTATGA AACTGAAAAC CTATCTCCTA GCTCTGCGTC CCTGGTCGCT 
0181 GTCCGCCAGT CTGGTTCCAA CGCTGCTCGG CTCAGCCCTG GCCTACCGCT CCCAGTGGGC 
0241 GGAGGAGTTT TCGCTGGCCA CCTTCTTTCT CACCGCCTTC ACCGTGGTCA CCGTCCACTG 
0301 CGCCGGCAAC GTAGTTAACA CCTATTTCGA CTTCATCAAG GGCATTGACA AGCAGAAAGC 
0361 CGACGACCGC ACGCTGGTGG ATCACATACT CACCAAGGAT GAGGTGGTAT CGCTGGGTGC 
0421 CATTCTGTAC ATGGCTGGCT GTGGAGGATT TGTTTTGCTG GCTGTGCTCA GTCCGGCGAA 
0481 AATGGAGCAC CTGGCACTGA

in silico PCR Fragment

0001 GTCCAGGAAT GGCAAGACCA AGACAGAGGA CGGTGAGGAG GTGGAAGCCG TTGTTGGAGC 
0061 TCGTGCAGCA GGTGCAGATG CCGGAGTAGC ACTAACGGGA CGACTAACAG GACACCCATC 
0121 CACATCCGGA ACGTTTATGA AACTGAAAAC CTATCTCCTA GCTCTGCGTC CCTGGTCGCT 
0181 GTCCGCCAGT CTGGTTCCAA CGCTGCTCGG CTCAGCCCTG GCCTACCGCT CCCAGTGGGC 
0241 GGAGGAGTTT TCGCTGGCCA CCTTCTTTCT CACCGCCTTC ACCGTGGTCA CCGTCCACTG 
0301 CGCCGGCAAC GTAGTTAACA CCTATTTCGA CTTCATCAAG GGCATTGACA AGCAGAAAGC 
0361 CGACGACCGC ACGCTGGTGG ATCACATACT CACCAAGGAT GAGGTGGTAT CGCTGGGTGC 
0421 CATTCTGTAC ATGGCTGGCT GTGGAGGATT TGTTTTGCTG GCTGTGCTCA GTCCGGCGAA 
0481 AATGGAGCAC CTGGCACTGA

Assemble Data


5876R-2.IR_full       1   GTCCAGGAATGGCAAGACCAAGACAGAGGACGGTGAGGAGGTGGAAGCCGTTGTTGGAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     1   GTCCAGGAATGGCAAGACCAAGACAGAGGACGGTGAGGAGGTGGAAGCCGTTGTTGGAGC 60


5876R-2.IR_full       61  TCGTGCAGCAGGTGCAGATGCCGGAGTAGCACTAACGGGACGACTAACAGGACACCCATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     61  TCGTGCAGCAGGTGCAGATGCCGGAGTAGCACTAACGGGACGACTAACAGGACACCCATC 120


5876R-2.IR_full       121 CACATCCGGAACGTTTATGAAACTGAAAACCTATCTCCTAGCTCTGCGTCCCTGGTCGCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     121 CACATCCGGAACGTTTATGAAACTGAAAACCTATCTCCTAGCTCTGCGTCCCTGGTCGCT 180


5876R-2.IR_full       181 GTCCGCCAGTCTGGTTCCAACGCTGCTCGGCTCAGCCCTGGCCTACCGCTCCCAGTGGGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     181 GTCCGCCAGTCTGGTTCCAACGCTGCTCGGCTCAGCCCTGGCCTACCGCTCCCAGTGGGC 240


5876R-2.IR_full       241 GGAGGAGTTTTCGCTGGCCACCTTCTTTCTCACCGCCTTCACCGTGGTCACCGTCCACTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     241 GGAGGAGTTTTCGCTGGCCACCTTCTTTCTCACCGCCTTCACCGTGGTCACCGTCCACTG 300


5876R-2.IR_full       301 CGCCGGCAACGTAGTTAACACCTATTTCGACTTCATCAAGGGCATTGACAAGCAGAAAGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     301 CGCCGGCAACGTAGTTAACACCTATTTCGACTTCATCAAGGGCATTGACAAGCAGAAAGC 360


5876R-2.IR_full       361 CGACGACCGCACGCTGGTGGATCACATACTCACCAAGGATGAGGTGGTATCGCTGGGTGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     361 CGACGACCGCACGCTGGTGGATCACATACTCACCAAGGATGAGGTGGTATCGCTGGGTGC 420


5876R-2.IR_full       421 CATTCTGTACATGGCTGGCTGTGGAGGATTTGTTTTGCTGGCTGTGCTCAGTCCGGCGAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5876R-2.in silico     421 CATTCTGTACATGGCTGGCTGTGGAGGATTTGTTTTGCTGGCTGTGCTCAGTCCGGCGAA 480


5876R-2.IR_full       481 AATGGAGCACCTGGCACTGA 500
                          ||||||||||||||||||||
5876R-2.in silico     481 AATGGAGCACCTGGCACTGA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_078857.2	CG5876-RA (heix), mRNA
0.41	2	2	2	3	NM_169758.1	CG5407-RA, transcript variant A (Sur-8), mRNA
0.41	2	2	2	3	NM_142363.4	CG5407-RB, transcript variant B (Sur-8), mRNA
0	0	3	3	3	NM_205957.1	CG33304-RA (rho-5), mRNA
0	0	3	2	2	NM_168235.1	CG17888-RB, transcript variant B (Pdp1), mRNA
0	0	3	2	2	NM_168236.1	CG17888-RG, transcript variant G (Pdp1), mRNA
0	0	2	7	9	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	2	4	7	NM_142395.1	CG7431-RA (CG7431), mRNA
0	0	2	2	3	NM_135833.2	CG16865-RA (CG16865), mRNA
0	0	1	6	6	NM_136687.2	CG1472-RA (sec24), mRNA
0	0	1	4	7	NM_133103.2	CG7280-RA (CG7280), mRNA
0	0	1	4	6	NM_142246.2	CG4225-RA (CG4225), mRNA
0	0	1	4	3	NM_057894.3	CG5799-RA, transcript variant A (dve), mRNA
0	0	1	3	3	NM_133156.1	CG12202-RA (Nat1), mRNA
0	0	1	2	6	NM_141376.2	CG15594-RA, transcript variant A (CG15594), mRNA
0	0	1	2	2	NM_206259.1	CG1271-RC, transcript variant C (CG1271), mRNA
0	0	1	2	2	NM_167973.1	CG1271-RD, transcript variant D (CG1271), mRNA
0	0	1	2	2	NM_139506.2	CG1271-RA, transcript variant A (CG1271), mRNA
0	0	1	2	2	NM_167974.1	CG1271-RB, transcript variant B (CG1271), mRNA
0	0	1	2	1	NM_168282.1	CG32351-RA (CG32351), mRNA
0	0	0	6	6	NM_139401.1	CG13933-RA (CG13933), mRNA
0	0	0	6	5	NM_169921.1	CG31203-RE, transcript variant E (CG31203), mRNA
0	0	0	6	5	NM_169920.1	CG31203-RD, transcript variant D (CG31203), mRNA
0	0	0	6	5	NM_169918.1	CG31203-RA, transcript variant A (CG31203), mRNA
0	0	0	6	5	NM_169919.1	CG31203-RB, transcript variant B (CG31203), mRNA
0	0	0	6	5	NM_169922.1	CG31203-RC, transcript variant C (CG31203), mRNA
0	0	0	5	14	NM_206407.1	CG32434-RA, transcript variant A (siz), mRNA
0	0	0	5	11	NM_168881.1	CG32434-RB, transcript variant B (siz), mRNA
0	0	0	5	11	NM_001043153.1	CG32434-RC, transcript variant C (siz), mRNA
0	0	0	5	10	NM_133045.2	CG15064-RA (Him), mRNA

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