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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5830R-2 
 Symbol CG5830  Full Name CG5830 
 CG No CG5830  Old CG No CG5830 
 Synonyms CG5830 
 Accession No (Link to NCBI) NM_140568.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Ibaraki K, Nakatsuka M, Ohsako T, Watanabe M, Miyazaki Y, Shirakami M, Karr TL, Sanuki R, Tomaru M, Takano-Shimizu-Kouno T.<br> A cross-species approach for the identification of Drosophila male sterility genes.<br> G3 (Bethesda) (2021) 11(8) [ PubMed ID = 34849808 ] [ RRC reference ]

Swarup S, Pradhan-Sundd T, Verheyen EM.<br> Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.<br> Development (2015) 142(8) 1502-15 [ PubMed ID = 25852200 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTACCTACTACCTCAGGTTAGG 
 Primer Seq. 3' 
aatctagaggtaccTCAAAGAGCGGGATCAGTTCG 
 Predicted Fragment Size 
494 
 IR fragment full Seq 
0001 TACCTACTAC CTCAGGTTAG GCTTACCGAT ATGCACAGAA AGTGCATGGT GATCGATTTG 
0061 GATGAGACCC TAGTGCACAG TAGTTTTAAG CCCATACCGA ATGCTGATTT CATAGTGCCC 
0121 GTGGAGATCG ATGGAACCGT ACATCAGGTG TACGTCCTGA ANGCGACCGC ACGTGGACGA 
0181 GTTTCTGCAG AAGATGGGCG AACTATACGA GTGCGTTCTG TTCACAGCAT CACTAGCCAA 
0241 ATACGCAGAT CCCGTCGCCG ATCTGTTAGA CAAGTGGAAT GTGTTTCGTG CAAGACTGTT 
0301 TAGGGAATCA TGCGTTTATT ACAGGGGTAA TTACATTAAG GATCTGAATC GTCTGGGGCG 
0361 GGACCTTCAG AAGATTGTCA TTGTCGACAA TTCACCGGCC AGTTATATCT TTCATCCAGA 
0421 CAATGCAGTT CCTGTTAAAT CCTGGTTTGA CGATGTCACC GACTGCGAAC TGCGCGAACT 
0481 GATCCCGCTC TTTGA 
 in silico PCR Fragment 
0001 TACCTACTAC CTCAGGTTAG GCTTACCGAT ATGCACAGAA AGTGCATGGT GATCGATTTG 
0061 GATGAGACCC TAGTGCACAG TAGTTTTAAG CCCATACCGA ATGCTGATTT CATAGTGCCC 
0121 GTGGAGATCG ATGGAACCGT ACATCAGGTG TACGTCCTGA A-GCGACCGC ACGTGGACGA 
0181 GTTTCTGCAG AAGATGGGCG AACTATACGA GTGCGTTCTG TTCACAGCAT CACTAGCCAA 
0241 ATACGCAGAT CCCGTCGCCG ATCTGTTAGA CAAGTGGAAT GTGTTTCGTG CAAGACTGTT 
0301 TAGGGAATCA TGCGTTTATT ACAGGGGTAA TTACATTAAG GATCTGAATC GTCTGGGGCG 
0361 GGACCTTCAG AAGATTGTCA TTGTCGACAA TTCACCGGCC AGTTATATCT TTCATCCAGA 
0421 CAATGCAGTT CCTGTTAAAT CCTGGTTTGA CGATGTCACC GACTGCGAAC TGCGCGAACT 
0481 GATCCCGCTC TTTGA 
 Assemble Data 
<PRE>

5830R-2.IR_full       1   TACCTACTACCTCAGGTTAGGCTTACCGATATGCACAGAAAGTGCATGGTGATCGATTTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5830R-2.in silico     1   TACCTACTACCTCAGGTTAGGCTTACCGATATGCACAGAAAGTGCATGGTGATCGATTTG 60


5830R-2.IR_full       61  GATGAGACCCTAGTGCACAGTAGTTTTAAGCCCATACCGAATGCTGATTTCATAGTGCCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5830R-2.in silico     61  GATGAGACCCTAGTGCACAGTAGTTTTAAGCCCATACCGAATGCTGATTTCATAGTGCCC 120


5830R-2.IR_full       121 GTGGAGATCGATGGAACCGTACATCAGGTGTACGTCCTGAA<span class="snp"><tt>N</tt></span>GCGACCGCACGTGGACGA 180
                          |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
5830R-2.in silico     121 GTGGAGATCGATGGAACCGTACATCAGGTGTACGTCCTGAA<span class="snp"><tt>-</tt></span>GCGACCGCACGTGGACGA 180


5830R-2.IR_full       181 GTTTCTGCAGAAGATGGGCGAACTATACGAGTGCGTTCTGTTCACAGCATCACTAGCCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5830R-2.in silico     181 GTTTCTGCAGAAGATGGGCGAACTATACGAGTGCGTTCTGTTCACAGCATCACTAGCCAA 240


5830R-2.IR_full       241 ATACGCAGATCCCGTCGCCGATCTGTTAGACAAGTGGAATGTGTTTCGTGCAAGACTGTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5830R-2.in silico     241 ATACGCAGATCCCGTCGCCGATCTGTTAGACAAGTGGAATGTGTTTCGTGCAAGACTGTT 300


5830R-2.IR_full       301 TAGGGAATCATGCGTTTATTACAGGGGTAATTACATTAAGGATCTGAATCGTCTGGGGCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5830R-2.in silico     301 TAGGGAATCATGCGTTTATTACAGGGGTAATTACATTAAGGATCTGAATCGTCTGGGGCG 360


5830R-2.IR_full       361 GGACCTTCAGAAGATTGTCATTGTCGACAATTCACCGGCCAGTTATATCTTTCATCCAGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5830R-2.in silico     361 GGACCTTCAGAAGATTGTCATTGTCGACAATTCACCGGCCAGTTATATCTTTCATCCAGA 420


5830R-2.IR_full       421 CAATGCAGTTCCTGTTAAATCCTGGTTTGACGATGTCACCGACTGCGAACTGCGCGAACT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5830R-2.in silico     421 CAATGCAGTTCCTGTTAAATCCTGGTTTGACGATGTCACCGACTGCGAACTGCGCGAACT 480


5830R-2.IR_full       481 GATCCCGCTCTTTGA 495
                          |||||||||||||||
5830R-2.in silico     481 GATCCCGCTCTTTGA 495

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   476  0  0  1  NM_140568.1  CG5830-RA (CG5830), mRNA 
0   0  1  3  3  NM_168142.1  CG32415-RA (CG32415), mRNA 
0   0  1  2  6  NM_133033.1  CG12672-RA (CG12672), mRNA 
0   0  1  2  4  NM_135852.2  CG4500-RA (CG4500), mRNA 
0   0  1  2  3  NM_166546.2  CG30092-RD, transcript variant D (jbug), mRNA 
0   0  1  2  2  NM_133123.1  CG7481-RA (RhoGAP18B), mRNA 
0   0  0  7  3  NM_135559.2  CG5337-RA (CG5337), mRNA 
0   0  0  5  2  NM_176065.1  CG1864-RC, transcript variant C (Hr38), mRNA 
0   0  0  5  2  NM_079100.2  CG17632-RA (bw), mRNA 
0   0  0  4  6  NM_176755.1  CG7122-RB, transcript variant B (RhoGAP16F), mRNA 
0   0  0  4  6  NM_133039.3  CG7122-RA, transcript variant A (RhoGAP16F), mRNA 
0   0  0  4  4  NM_168217.2  CG7546-RB, transcript variant B (CG7546), mRNA 
0   0  0  4  4  NM_139875.3  CG7546-RA, transcript variant A (CG7546), mRNA 
0   0  0  4  3  NM_079642.2  CG6177-RA (ldlCp), mRNA 
0   0  0  4  3  NM_134770.1  CG17657-RA (CG17657), mRNA 
0   0  0  4  3  NM_138250.2  CG9149-RA (CG9149), mRNA 
0   0  0  3  7  NM_143769.3  CG9432-RA, transcript variant A (l(2)01289), mRNA 
0   0  0  3  6  NM_164695.1  CG11050-RC, transcript variant C (CG11050), mRNA 
0   0  0  3  6  NM_164694.1  CG11050-RB, transcript variant B (CG11050), mRNA 
0   0  0  3  6  NM_135208.4  CG11050-RA, transcript variant A (CG11050), mRNA 
0   0  0  3  5  NM_134605.2  CG1696-RA (l(1)G0269), mRNA 
0   0  0  3  4  NM_166248.1  CG5033-RB, transcript variant B (CG5033), mRNA 
0   0  0  3  4  NM_137426.2  CG5033-RA, transcript variant A (CG5033), mRNA 
0   0  0  3  4  NM_057899.2  CG3991-RA, transcript variant A (TppII), mRNA 
0   0  0  3  4  NM_165966.1  CG3991-RD, transcript variant D (TppII), mRNA 
0   0  0  3  3  NM_168781.1  CG3961-RA, transcript variant A (CG3961), mRNA 
0   0  0  3  3  NM_080063.2  CG1469-RA, transcript variant A (Fer2LCH), mRNA 
0   0  0  3  3  NM_170483.1  CG1469-RB, transcript variant B (Fer2LCH), mRNA 
0   0  0  3  3  NM_170484.1  CG1469-RC, transcript variant C (Fer2LCH), mRNA 
0   0  0  3  3  NM_140810.2  CG3961-RC, transcript variant C (CG3961), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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