|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5789R-1 |
| Symbol |
CG5789 |
Full Name |
CG5789 |
| CG No |
CG5789 |
Old CG No |
CG5789 |
| Synonyms |
Dme_CG5789, CG5789 |
| Accession No (Link to NCBI) |
NM_143012.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Lane EA, Petsakou A, Liu Y, Chen W, Qadiri M, Hu Y, Perrimon N.
Cholinergic signaling modulates intestinal pathophysiology in a Drosophila model of cystic fibrosis.
PLoS Genet (2026) 22(2) e1012048
[
PubMed ID = 41701793
]
[
RRC reference
]
Kim K, Lane EA, Saftien A, Wang H, Xu Y, Wirtz-Peitz F, Perrimon N.
Drosophila as a model for studying cystic fibrosis pathophysiology of the gastrointestinal system.
Proc Natl Acad Sci U S A (2020) 117(19) 10357-10367
[
PubMed ID = 32345720
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGAGCCGATTCAGCCTCCAG |
| Primer Seq. 3' |
aatctagaggtaccCTCCGCAATTCTCATCCGCAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGAGCCGAT TCAGCCTCCA GTAGAGGAAG TGGATCACAA TGCAACCGCT GTGCGAAGCA
0061 TCNTTGGATC AGACCGTCGA GCACATGTGG AACGATATGT ACAGTCTGGG CGCCGTCCTG
0121 GTTCTTTCGA CGCTGTTCTG CTGCTTCCTG TTGCACCACG TCGATCTCCG ACAGCGCCTA
0181 ATGGGTGCAC GGATGCGAAT AGCCTGCTGC TCGCTGATCT ACCGGAAAAC TCTACGGCTC
0241 TCGATGAAAA CGGCTGGTCA GACGCCAGCT GGTTACCTAA TCAACCTGCT CTCGAATGAC
0301 GTGAATCGTT TGGACTATGG CTTCATATTC ATGCACTGGA TCTGGATTAT GCCATTGCAG
0361 GCGATGCTCA CATGCTACCT GATTTGGCTG AACATTGGCA TCCCAGCAGT TGTGGGTGTA
0421 GTTGGACTGC TGCTAAAAAC GGTGCCCGTG CAGACGGCCT TGAGCAAGGT AACCTCTGTG
0481 CTGCGGATGA GAATTGCGGA G |
| in silico PCR Fragment |
0001 TGGAGCCGAT TCAGCCTCCA GTAGAGGAAG TGGATCACAA TGCAACCGCT GTGCGAAGCA
0061 TC-TTGGATC AGACCGTCGA GCACATGTGG AACGATATGA ACAGTCTGGG CGCCGTCCTG
0121 GTTCTTTCGA CGCTGTTCTG CTGCTTCCTG TTGCACCACG TCGATCTCCG ACAGCGCCTA
0181 ATGGGTGCAC GGATGCGAAT AGCCTGCTGC TCGCTGATCT ACCGGAAAAC TCTACGGCTC
0241 TCGATGAAAA CGGCTGGTCA GACGCCAGCT GGTTACCTAA TCAACCTTCT TTCGAATGAT
0301 GTGAATCGTT TGGACTATGG CTTCATATTC ATGCACTGGA TCTGGATTAT GCCATTGCAG
0361 GCGATGCTCA CGTGCTACCT GATTTGGCTG AACATTGGCA TCCCAGCAAT TGTGGGTGTA
0421 GTTGGACTGC TGCTAAAAAC GGTGCCCGTG CAGACGGCCT TGAGCAAGGT AACCTCTGTG
0481 CTGCGGATGA GAATTGCGGA G |
| Assemble Data |
5789R-1.IR_full 1 TGGAGCCGATTCAGCCTCCAGTAGAGGAAGTGGATCACAATGCAACCGCTGTGCGAAGCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico 1 TGGAGCCGATTCAGCCTCCAGTAGAGGAAGTGGATCACAATGCAACCGCTGTGCGAAGCA 60
5789R-1.IR_full 61 TCNTTGGATCAGACCGTCGAGCACATGTGGAACGATATGTACAGTCTGGGCGCCGTCCTG 120
|| |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
5789R-1.in silico 61 TC-TTGGATCAGACCGTCGAGCACATGTGGAACGATATGAACAGTCTGGGCGCCGTCCTG 120
5789R-1.IR_full 121 GTTCTTTCGACGCTGTTCTGCTGCTTCCTGTTGCACCACGTCGATCTCCGACAGCGCCTA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico 121 GTTCTTTCGACGCTGTTCTGCTGCTTCCTGTTGCACCACGTCGATCTCCGACAGCGCCTA 180
5789R-1.IR_full 181 ATGGGTGCACGGATGCGAATAGCCTGCTGCTCGCTGATCTACCGGAAAACTCTACGGCTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico 181 ATGGGTGCACGGATGCGAATAGCCTGCTGCTCGCTGATCTACCGGAAAACTCTACGGCTC 240
5789R-1.IR_full 241 TCGATGAAAACGGCTGGTCAGACGCCAGCTGGTTACCTAATCAACCTGCTCTCGAATGAC 300
||||||||||||||||||||||||||||||||||||||||||||||| || ||||||||
5789R-1.in silico 241 TCGATGAAAACGGCTGGTCAGACGCCAGCTGGTTACCTAATCAACCTTCTTTCGAATGAT 300
5789R-1.IR_full 301 GTGAATCGTTTGGACTATGGCTTCATATTCATGCACTGGATCTGGATTATGCCATTGCAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico 301 GTGAATCGTTTGGACTATGGCTTCATATTCATGCACTGGATCTGGATTATGCCATTGCAG 360
5789R-1.IR_full 361 GCGATGCTCACATGCTACCTGATTTGGCTGAACATTGGCATCCCAGCAGTTGTGGGTGTA 420
||||||||||| |||||||||||||||||||||||||||||||||||| |||||||||||
5789R-1.in silico 361 GCGATGCTCACGTGCTACCTGATTTGGCTGAACATTGGCATCCCAGCAATTGTGGGTGTA 420
5789R-1.IR_full 421 GTTGGACTGCTGCTAAAAACGGTGCCCGTGCAGACGGCCTTGAGCAAGGTAACCTCTGTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico 421 GTTGGACTGCTGCTAAAAACGGTGCCCGTGCAGACGGCCTTGAGCAAGGTAACCTCTGTG 480
5789R-1.IR_full 481 CTGCGGATGAGAATTGCGGAG 501
|||||||||||||||||||||
5789R-1.in silico 481 CTGCGGATGAGAATTGCGGAG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_143012.1 |
CG5789-RA (CG5789), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_143318.2 |
CG31057-RA (tau), mRNA |
| 0 |
0 |
1 |
7 |
9 |
NM_166876.1 |
CG14622-RB, transcript variant B (DAAM), mRNA |
| 0 |
0 |
1 |
7 |
9 |
NM_166875.1 |
CG14622-RC, transcript variant C (DAAM), mRNA |
| 0 |
0 |
1 |
7 |
7 |
NM_130544.3 |
CG14622-RA, transcript variant A (DAAM), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_133149.2 |
CG8051-RA (CG8051), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168353.1 |
CG32048-RB, transcript variant B (CG32048), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168352.1 |
CG32048-RA, transcript variant A (CG32048), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_136955.2 |
CG8815-RA, transcript variant A (Sin3A), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165916.1 |
CG8815-RC, transcript variant C (Sin3A), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165915.1 |
CG8815-RB, transcript variant B (Sin3A), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_164653.1 |
CG31646-RA (CG31646), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_139659.1 |
CG11357-RA (CG11357), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136935.1 |
CG8834-RA (CG8834), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_168048.1 |
CG32261-RA (Gr64a), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_134595.2 |
CG1724-RA (CG1724), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_139612.1 |
CG1308-RA (CG1308), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206253.1 |
CG12002-RE, transcript variant E (Pxn), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206254.1 |
CG12002-RD, transcript variant D (Pxn), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206255.1 |
CG12002-RC, transcript variant C (Pxn), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079167.3 |
CG12002-RA, transcript variant A (Pxn), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_141996.2 |
CG12360-RA, transcript variant A (CG12360), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169223.2 |
CG31369-RA (PQBP-1), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_079901.2 |
CG10811-RA (eIF-4G), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_132576.1 |
CG15730-RA (CG15730), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_176445.1 |
CG33208-RC, transcript variant C (MICAL), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_176449.1 |
CG33208-RH, transcript variant H (MICAL), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_176448.1 |
CG33208-RG, transcript variant G (MICAL), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_176446.1 |
CG33208-RD, transcript variant D (MICAL), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_176447.1 |
CG33208-RF, transcript variant F (MICAL), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
