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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

5704R-2

Symbol

CG5704

Full Name

CG5704

CG No

CG5704

Old CG No

CG5704

Synonyms

LD30049, BEST:LD30049, CG5704

Accession No (Link to NCBI)

NM_143811.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Enli L, Moronuki Y, Yamada T, Kose H. Examination of Niddm20 candidate genes of OLETF rats in Drosophila melanogaster using inducible GeneSwitch GAL4 system. J Genet (2022) 101 [ PubMed ID = 35129134 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGGAACTTTGTCACTGAGC

Primer Seq. 3'

aatctagaggtaccGAGGGCGGCTCAATGTAATTT

Predicted Fragment Size

IR fragment full Seq

0001 ATGGGAACTT TGTCACTGAG CGATTTCGAA GCTGTGAAGA TTCCCGTACC CTGGGGTCAC 
0061 ATTTCTGGAC GTTGGTACGG AAATCGGAAC GAGCGACCCA TCTTGGCGAT TCACGGATGG 
0121 CTGGACAACC TGGGAACCTT CGATCGGCTC ATTCCCCTGC TTCCTGACTA TCTAGGAGTA 
0181 CTCTGCATCG ATCTGCCAGG ACATGGAAGA TCCTCGCATT TGCCACCGGG AATGTACTAC 
0241 AGTGTGTACG AGTACGTGTT CACTATTCCA CTGGTCATGA AGGAGTACGG ATGGTCAAAG 
0301 GTCTCTCTCA TTGGACACTC ACTGGGCGGA GTCCTGAGCT TCATTTATGC CTCTCTTGCT 
0361 CCTCACACCG TCGATATGAT CGTCTCCCTG GNACATACTG CTCCCCTTGA GAAACGACAT 
0421 CGACTACATG GNACTCTTAG TATAGAGAAA CAACTAGTGA ATGTTGAGCG ACAGAAGCTG 
0481 GGAAATTACA TTTGANGCCG CCCTc

in silico PCR Fragment

0001 ATGGGAACTT TGTCACTGAG CGATTTCGAA GCTGTGAAGA TTCCCGTACC CTGGGGTCAC 
0061 ATTTCTGGAC GTTGGTACGG AAATCGGAAC GAGCGACCCA TCTTGGCGAT TCACGGATGG 
0121 CTGGACAACC TGGGAACCTT CGATCGGCTC ATTCCCCTGC TTCCTGACTA TCTAGGAGTA 
0181 CTCTGCATCG ATCTGCCAGG ACATGGAAGA TCCTCGCATT TGCCACCGGG AATGTACTAC 
0241 AGTGTGTACG AGTACGTGTT CACTATTCCA CTGGTCATGA AGGAGTACGG ATGGTCAAAG 
0301 GTCTCTCTCA TTGGACACTC ACTGGGCGGA GTCCTGAGCT TCATTTATGC CTCTCTTGCT 
0361 CCTCACACCG TCGATATGAT CGTCTCCCTG G-ACATACTG CTCCCCTTGA GAAACGACAT 
0421 AGACTACATG G-AC-CTTAG TATAGAGAAA CAACTAGTGA ATGTTGAGCG ACAGAAGCTG 
0481 GGAAATTACA -TTGA-GCCG CCCTC

Assemble Data

<PRE>

5704R-2.IR_full       1   ATGGGAACTTTGTCACTGAGCGATTTCGAAGCTGTGAAGATTCCCGTACCCTGGGGTCAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5704R-2.in silico     1   ATGGGAACTTTGTCACTGAGCGATTTCGAAGCTGTGAAGATTCCCGTACCCTGGGGTCAC 60


5704R-2.IR_full       61  ATTTCTGGACGTTGGTACGGAAATCGGAACGAGCGACCCATCTTGGCGATTCACGGATGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5704R-2.in silico     61  ATTTCTGGACGTTGGTACGGAAATCGGAACGAGCGACCCATCTTGGCGATTCACGGATGG 120


5704R-2.IR_full       121 CTGGACAACCTGGGAACCTTCGATCGGCTCATTCCCCTGCTTCCTGACTATCTAGGAGTA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5704R-2.in silico     121 CTGGACAACCTGGGAACCTTCGATCGGCTCATTCCCCTGCTTCCTGACTATCTAGGAGTA 180


5704R-2.IR_full       181 CTCTGCATCGATCTGCCAGGACATGGAAGATCCTCGCATTTGCCACCGGGAATGTACTAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5704R-2.in silico     181 CTCTGCATCGATCTGCCAGGACATGGAAGATCCTCGCATTTGCCACCGGGAATGTACTAC 240


5704R-2.IR_full       241 AGTGTGTACGAGTACGTGTTCACTATTCCACTGGTCATGAAGGAGTACGGATGGTCAAAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5704R-2.in silico     241 AGTGTGTACGAGTACGTGTTCACTATTCCACTGGTCATGAAGGAGTACGGATGGTCAAAG 300


5704R-2.IR_full       301 GTCTCTCTCATTGGACACTCACTGGGCGGAGTCCTGAGCTTCATTTATGCCTCTCTTGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5704R-2.in silico     301 GTCTCTCTCATTGGACACTCACTGGGCGGAGTCCTGAGCTTCATTTATGCCTCTCTTGCT 360


5704R-2.IR_full       361 CCTCACACCGTCGATATGATCGTCTCCCTGG<span class="snp"><tt>N</tt></span>ACATACTGCTCCCCTTGAGAAACGACAT 420
                          |||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||
5704R-2.in silico     361 CCTCACACCGTCGATATGATCGTCTCCCTGG<span class="snp"><tt>-</tt></span>ACATACTGCTCCCCTTGAGAAACGACAT 420


5704R-2.IR_full       421 <span class="snp"><tt>C</tt></span>GACTACATGG<span class="snp"><tt>N</tt></span>AC<span class="snp"><tt>T</tt></span>CTTAGTATAGAGAAACAACTAGTGAATGTTGAGCGACAGAAGCTG 480
                          <span class="snp"><tt>&nbsp;</tt></span>||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||
5704R-2.in silico     421 <span class="snp"><tt>A</tt></span>GACTACATGG<span class="snp"><tt>-</tt></span>AC<span class="snp"><tt>-</tt></span>CTTAGTATAGAGAAACAACTAGTGAATGTTGAGCGACAGAAGCTG 480


5704R-2.IR_full       481 GGAAATTACA<span class="snp"><tt>T</tt></span>TTGA<span class="snp"><tt>N</tt></span>GCCGCCCTC 505
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
5704R-2.in silico     481 GGAAATTACA<span class="snp"><tt>-</tt></span>TTGA<span class="snp"><tt>-</tt></span>GCCGCCCTC 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_143811.2	CG5704-RA (CG5704), mRNA
1.86	9	42	44	47	NM_139437.1	CG15879-RA (CG15879), mRNA
1.86	9	18	32	39	NR_001931.1	CR15821, mRNA
0.82	4	42	15	40	NM_139439.2	CG15820-RA (CG15820), mRNA
0	0	4	6	17	NM_143808.2	CG5707-RA (CG5707), mRNA
0	0	3	2	2	NM_170576.1	CG1945-RC, transcript variant C (faf), mRNA
0	0	3	2	2	NM_079873.3	CG1945-RA, transcript variant A (faf), mRNA
0	0	2	4	6	NM_165836.1	CG7776-RB, transcript variant B (E(Pc)), mRNA
0	0	2	4	6	NM_078974.2	CG7776-RA, transcript variant A (E(Pc)), mRNA
0	0	2	4	2	NM_170581.1	CG31000-RB, transcript variant B (heph), mRNA
0	0	2	4	2	NM_170582.2	CG31000-RA, transcript variant A (heph), mRNA
0	0	2	4	2	NM_176597.1	CG31000-RE, transcript variant E (heph), mRNA
0	0	2	4	2	NM_176601.1	CG31000-RJ, transcript variant J (heph), mRNA
0	0	2	4	2	NM_176602.1	CG31000-RK, transcript variant K (heph), mRNA
0	0	2	4	2	NM_176596.1	CG31000-RD, transcript variant D (heph), mRNA
0	0	2	4	2	NM_176603.1	CG31000-RH, transcript variant H (heph), mRNA
0	0	2	4	2	NM_176599.1	CG31000-RG, transcript variant G (heph), mRNA
0	0	2	4	2	NM_176598.1	CG31000-RF, transcript variant F (heph), mRNA
0	0	2	4	2	NM_079964.2	CG31000-RC, transcript variant C (heph), mRNA
0	0	2	4	2	NM_176600.1	CG31000-RI, transcript variant I (heph), mRNA
0	0	2	3	2	NM_057277.2	CG8694-RA (LvpD), mRNA
0	0	2	2	4	NM_136453.1	CG2105-RA, transcript variant A (Corin), mRNA
0	0	2	2	4	NM_206051.1	CG2105-RB, transcript variant B (Corin), mRNA
0	0	1	4	3	NM_134808.2	CG10874-RA, transcript variant A (CG10874), mRNA
0	0	1	4	3	NM_164458.1	CG10874-RB, transcript variant B (CG10874), mRNA
0	0	1	3	3	NM_138136.2	CG30427-RB, transcript variant B (CG30427), mRNA
0	0	1	3	2	NM_132058.2	CG5966-RA (CG5966), mRNA
0	0	1	3	2	NM_134817.1	CG4267-RA (CG4267), mRNA
0	0	1	2	4	NM_136421.1	CG1850-RA (CG1850), mRNA
0	0	1	2	3	NM_078698.2	CG1676-RA (cactin), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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