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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5688R-1 |
| Symbol |
Grip163 |
Full Name |
Grip163 |
| CG No |
CG5688 |
Old CG No |
CG5688 |
| Synonyms |
Dgrip163, Dgrips163, grip163, CG5688, Grip163 |
| Accession No (Link to NCBI) |
NM_140280.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.<br> The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in <i>Drosophila</i>.<br> Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCCGATGAGATGAAGCCAAAA |
| Primer Seq. 3' |
aatctagaggtaccGACTCTGATAATCCCATGCGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCCGNTGAGA TGAAGCCAAA AAAACAAACA GCTGtGNTGG TGGCCAAAAA GCGCTCCAAA
0061 ATCTATGAAT CGCTGCTTCA CAGTGAAACG ATGTCGTCCC AGGACAAGCT GGAGATGCTC
0121 CCACCAGGTG ACCAGCGAGG TGGCAAAGAA CCCAGACCGG ATGGACATGC TGCTCAGGTT
0181 CCAAAATCTG CTAGAAGGAC AGGATAGGGA AGAATCGGAG ATTACACAGG ACCAGGATGA
0241 GGAAAACCAG TCCGAAGTGG TGATCAAACG TATCAAGGCA GGTCCACTGC AGCTGATTGA
0301 AACCATAGAG ATTGTGGAGG AGGCACTTCA TCCAAAGGCC TTTCCCGACA TCAATCAAAT
0361 GAATCGCGCC CTGAAACCCC TGCAACTACA GCCATACTCC GATATTCTTA CAACGAGTAA
0421 CCTGAATATA AATTTTCCGC CAGAAAAGCT AATTCCGAAG ATCATACCAT CTACTGTAAT
0481 TCGCATGGGA TTATCAGAGT C |
| in silico PCR Fragment |
0001 GCCGATGAGA TGAAGCCAAA AAAACAAACA GCTGTGCTGG TGGCCAAAAA GCGCTCCAAA
0061 ATCTATGAAT CGCTGCTTCA CAGTGAAACG ATGTCGTCCC AGGACAAGCT GGAGATGCT-
0121 CCACCAGGTG ACCAGCGAGG TGGCAAAGAA CCCAGACCGG ATGGACATGC TGCTCAGGTT
0181 CCAAAATCTG CTAGAAGGAC AGGATAAGGA AGAATCGGAG ATTACACAGG ACCAGGATGA
0241 GGAAAACCAG TCCGAAGTGG TGATCAAACG TATCAAGGCA GGTCCACTGC AGCTGATTGA
0301 AACCATAGAG ATTGTGGAGG AGGCACTTCA TCCAAAGGCC TTTCCCGACA TCAATCAAAT
0361 GAATCGCGCC CTGAAACCCC TGCAACTACA GCCATACTCC GATATTCTTA CAACGAGTAA
0421 CCTGAATATA AATTTTCCGC CAGAAAAGCT AATTCCGAAG ATCATACCAT CTACTGTAAT
0481 TCGCATGGGA TTATCAGAGT C |
| Assemble Data |
<PRE>
5688R-1.IR_full 1 GCCG<span class="snp"><tt>N</tt></span>TGAGATGAAGCCAAAAAAACAAACAGCTGTG<span class="snp"><tt>N</tt></span>TGGTGGCCAAAAAGCGCTCCAAA 60
||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
5688R-1.in silico 1 GCCG<span class="snp"><tt>A</tt></span>TGAGATGAAGCCAAAAAAACAAACAGCTGTG<span class="snp"><tt>C</tt></span>TGGTGGCCAAAAAGCGCTCCAAA 60
5688R-1.IR_full 61 ATCTATGAATCGCTGCTTCACAGTGAAACGATGTCGTCCCAGGACAAGCTGGAGATGCT<span class="snp"><tt>C</tt></span> 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
5688R-1.in silico 61 ATCTATGAATCGCTGCTTCACAGTGAAACGATGTCGTCCCAGGACAAGCTGGAGATGCT<span class="snp"><tt>-</tt></span> 120
5688R-1.IR_full 121 CCACCAGGTGACCAGCGAGGTGGCAAAGAACCCAGACCGGATGGACATGCTGCTCAGGTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5688R-1.in silico 121 CCACCAGGTGACCAGCGAGGTGGCAAAGAACCCAGACCGGATGGACATGCTGCTCAGGTT 180
5688R-1.IR_full 181 CCAAAATCTGCTAGAAGGACAGGATA<span class="snp"><tt>G</tt></span>GGAAGAATCGGAGATTACACAGGACCAGGATGA 240
||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||
5688R-1.in silico 181 CCAAAATCTGCTAGAAGGACAGGATA<span class="snp"><tt>A</tt></span>GGAAGAATCGGAGATTACACAGGACCAGGATGA 240
5688R-1.IR_full 241 GGAAAACCAGTCCGAAGTGGTGATCAAACGTATCAAGGCAGGTCCACTGCAGCTGATTGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5688R-1.in silico 241 GGAAAACCAGTCCGAAGTGGTGATCAAACGTATCAAGGCAGGTCCACTGCAGCTGATTGA 300
5688R-1.IR_full 301 AACCATAGAGATTGTGGAGGAGGCACTTCATCCAAAGGCCTTTCCCGACATCAATCAAAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5688R-1.in silico 301 AACCATAGAGATTGTGGAGGAGGCACTTCATCCAAAGGCCTTTCCCGACATCAATCAAAT 360
5688R-1.IR_full 361 GAATCGCGCCCTGAAACCCCTGCAACTACAGCCATACTCCGATATTCTTACAACGAGTAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5688R-1.in silico 361 GAATCGCGCCCTGAAACCCCTGCAACTACAGCCATACTCCGATATTCTTACAACGAGTAA 420
5688R-1.IR_full 421 CCTGAATATAAATTTTCCGCCAGAAAAGCTAATTCCGAAGATCATACCATCTACTGTAAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5688R-1.in silico 421 CCTGAATATAAATTTTCCGCCAGAAAAGCTAATTCCGAAGATCATACCATCTACTGTAAT 480
5688R-1.IR_full 481 TCGCATGGGATTATCAGAGTC 501
|||||||||||||||||||||
5688R-1.in silico 481 TCGCATGGGATTATCAGAGTC 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_140280.2 |
CG5688-RA (Grip163), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_206059.1 |
CG8083-RC, transcript variant C (CG8083), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_206060.1 |
CG8083-RB, transcript variant B (CG8083), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_136602.3 |
CG8083-RA, transcript variant A (CG8083), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_079705.2 |
CG5760-RA (rtet), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_079609.2 |
CG6019-RA (mus308), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_139356.1 |
CG3524-RA (v(2)k05816), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_143367.2 |
CG10011-RA (CG10011), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_057245.2 |
CG6189-RA (l(1)1Bi), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137912.1 |
CG9876-RA (CG9876), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_078662.2 |
CG5488-RA (B-H2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_138984.2 |
CG5712-RA (ACXD), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_205923.1 |
CG7231-RC, transcript variant C (CG7231), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_133083.1 |
CG15040-RA (CG15040), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_143623.1 |
CG1804-RA (kek6), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_142986.2 |
CG5720-RA (CG5720), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001014579.1 |
CG4665-RB, transcript variant B (Dhpr), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001014580.1 |
CG4665-RC, transcript variant C (Dhpr), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_079266.3 |
CG4665-RA, transcript variant A (Dhpr), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_135225.1 |
CG11323-RA (CG11323), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_170258.2 |
CG32474-RA (dys), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079887.2 |
CG1901-RA, transcript variant A (mav), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001014690.1 |
CG1901-RB, transcript variant B (mav), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_170016.1 |
CG5374-RB, transcript variant B (T-cp1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079726.3 |
CG5374-RA, transcript variant A (T-cp1), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_142448.2 |
CG8064-RA (CG8064), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176210.1 |
CG33197-RD, transcript variant D (mbl), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169140.1 |
CG10277-RC, transcript variant C (CG10277), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_170232.1 |
CG5071-RA, transcript variant A (CG5071), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_143149.1 |
CG5071-RB, transcript variant B (CG5071), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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