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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5568R-3 
 Symbol CG5568  Full Name CG5568 
 CG No CG5568  Old CG No CG5568 
 Synonyms CG5568 
 Accession No (Link to NCBI) NM_139735.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGCGTAAACTGGGTCTGGAG 
 Primer Seq. 3' 
aatctagaggtaccATGCCTGATGCCCAATCCAAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGCGTAAAC TGGGTCTGGA GCAAGGTGAC TTTGTGGGAG TCATTGGCAG GTTGACCACT 
0061 CACTTGACTG CTCTGGCCTA TGCCTGTTTC TTTAATGGAA CTCCTTATCA TGCCCTACAC 
0121 ACCGAATACG AACAATCTGC CATCGAGAGA CTGTTCGGAA TAACCAAGCC ACGCTTAATC 
0181 TTCTGTGATG GAGATGAGTT TGAGAAAGTT CAAGCAGCCA CCAAAGGCCT TCAGGTACAA 
0241 ATAGTAACCA TGCGTAATCA CCCGGCAGGA ATATTGAGAA TTCAGGATAT TTTAACCACT 
0301 CCCGTGGAGA TGAACTTTCG ACCAGTGCGA CTGAAAGACG GTACTGATCA ACTACTGGCC 
0361 ATTCTTAGCT CTTCGGGAAC ATCCGGCCTT CCAAAGGCAG TCACCATTAG CAATAGTCAC 
0421 CAGATTATTG GNGAGTTTTC TACCCGTGGA TAGCTCGATT ATTCAGTATA ACCCAAACAC 
0481 TTTGGATTGG GCATCAGGCA T 
 in silico PCR Fragment 
0001 ATGCGTAAAC TGGGTCTGGA GCAAGGTGAC TTTGTGGGAG TCATTGGCAG GTTGACCACT 
0061 CACTTGACTG CTCTGGCCTA TGCCTGTTTC TTTAATGGAA CTCCTTATCA TGCCCTACAC 
0121 ACCGAATACG AACAATCTGC CATCGAGAGA CTGTTCGGAA TAACCAAGCC ACGCTTAATC 
0181 TTCTGTGATG GAGATGAGTT TGAGAAAGTT CAAGCAGCCA CCAAAGGCCT TCAGGTACAA 
0241 ATAGTAACCA TGCGTAATCA CCCGGCAGGA ATATTGAGAA TTCAGGATAT TTTAACCACT 
0301 CCCGTGGAGA TGAACTTTCG ACCAGTGCGA CTGAAAGACG GTACTGATCA ACTACTGGCC 
0361 ATTCTTAGCT CTTCGGGAAC ATCCGGCCTT CCAAAGGCAG TCACCATTAG CAATAGTCAC 
0421 CAGATTATTG G-GAGTTTTC TACCCGTGGA TAGCTCGATT ATTCAGTATA ACCCAAACAC 
0481 TTTGGATTGG GCATCAGGCA T 
 Assemble Data 
<PRE>

5568R-3.IR_full       1   ATGCGTAAACTGGGTCTGGAGCAAGGTGACTTTGTGGGAGTCATTGGCAGGTTGACCACT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     1   ATGCGTAAACTGGGTCTGGAGCAAGGTGACTTTGTGGGAGTCATTGGCAGGTTGACCACT 60


5568R-3.IR_full       61  CACTTGACTGCTCTGGCCTATGCCTGTTTCTTTAATGGAACTCCTTATCATGCCCTACAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     61  CACTTGACTGCTCTGGCCTATGCCTGTTTCTTTAATGGAACTCCTTATCATGCCCTACAC 120


5568R-3.IR_full       121 ACCGAATACGAACAATCTGCCATCGAGAGACTGTTCGGAATAACCAAGCCACGCTTAATC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     121 ACCGAATACGAACAATCTGCCATCGAGAGACTGTTCGGAATAACCAAGCCACGCTTAATC 180


5568R-3.IR_full       181 TTCTGTGATGGAGATGAGTTTGAGAAAGTTCAAGCAGCCACCAAAGGCCTTCAGGTACAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     181 TTCTGTGATGGAGATGAGTTTGAGAAAGTTCAAGCAGCCACCAAAGGCCTTCAGGTACAA 240


5568R-3.IR_full       241 ATAGTAACCATGCGTAATCACCCGGCAGGAATATTGAGAATTCAGGATATTTTAACCACT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     241 ATAGTAACCATGCGTAATCACCCGGCAGGAATATTGAGAATTCAGGATATTTTAACCACT 300


5568R-3.IR_full       301 CCCGTGGAGATGAACTTTCGACCAGTGCGACTGAAAGACGGTACTGATCAACTACTGGCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     301 CCCGTGGAGATGAACTTTCGACCAGTGCGACTGAAAGACGGTACTGATCAACTACTGGCC 360


5568R-3.IR_full       361 ATTCTTAGCTCTTCGGGAACATCCGGCCTTCCAAAGGCAGTCACCATTAGCAATAGTCAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     361 ATTCTTAGCTCTTCGGGAACATCCGGCCTTCCAAAGGCAGTCACCATTAGCAATAGTCAC 420


5568R-3.IR_full       421 CAGATTATTGG<span class="snp"><tt>N</tt></span>GAGTTTTCTACCCGTGGATAGCTCGATTATTCAGTATAACCCAAACAC 480
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
5568R-3.in silico     421 CAGATTATTGG<span class="snp"><tt>-</tt></span>GAGTTTTCTACCCGTGGATAGCTCGATTATTCAGTATAACCCAAACAC 480


5568R-3.IR_full       481 TTTGGATTGGGCATCAGGCAT 501
                          |||||||||||||||||||||
5568R-3.in silico     481 TTTGGATTGGGCATCAGGCAT 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_139735.2  CG5568-RA (CG5568), mRNA 
0.41   2  11  18  25  NM_142572.1  CG11659-RA (CG11659), mRNA 
0.41   2  11  18  25  NM_142571.1  CG6300-RA (CG6300), mRNA 
0   0  3  2  7  NM_168293.1  CG32030-RA, transcript variant A (CG32030), mRNA 
0   0  3  2  7  NM_168294.1  CG32030-RB, transcript variant B (CG32030), mRNA 
0   0  3  2  2  NM_139978.3  CG6781-RA (CG6781), mRNA 
0   0  2  4  6  NM_132529.2  CG2025-RA (CG2025), mRNA 
0   0  2  3  4  NM_137077.2  CG13350-RA (CG13350), mRNA 
0   0  1  35  47  NM_139736.1  CG18586-RA (CG18586), mRNA 
0   0  1  14  29  NM_142574.2  CG11407-RA (CG11407), mRNA 
0   0  1  4  3  NM_165075.1  CG31839-RA (CG31839), mRNA 
0   0  1  3  3  NM_169430.2  CG6939-RB, transcript variant B (Sbf), mRNA 
0   0  1  3  3  NM_058053.3  CG6939-RA, transcript variant A (Sbf), mRNA 
0   0  1  2  4  NM_140007.1  CG5747-RA (mfr), mRNA 
0   0  1  2  2  NM_079230.2  CG32386-RA (corn), mRNA 
0   0  0  4  3  NM_079589.2  CG6649-RA (Ugt35b), mRNA 
0   0  0  4  3  NM_139928.3  CG7999-RA (MED24), mRNA 
0   0  0  3  9  NM_139443.1  CG13809-RA (osm-1), mRNA 
0   0  0  3  8  NM_176325.1  CG8865-RB, transcript variant B (Rgl), mRNA 
0   0  0  3  8  NM_176327.1  CG8865-RD, transcript variant D (Rgl), mRNA 
0   0  0  3  8  NM_079958.2  CG8865-RA, transcript variant A (Rgl), mRNA 
0   0  0  3  8  NM_176326.1  CG8865-RC, transcript variant C (Rgl), mRNA 
0   0  0  3  6  NM_169202.1  CG11094-RA, transcript variant A (dsx), mRNA 
0   0  0  3  6  NM_142828.1  CG13842-RA (CG13842), mRNA 
0   0  0  3  4  NM_058035.2  CG8717-RA (slv), mRNA 
0   0  0  3  3  NM_140772.2  CG7341-RA, transcript variant A (CG7341), mRNA 
0   0  0  3  3  NM_168763.1  CG7341-RB, transcript variant B (CG7341), mRNA 
0   0  0  3  3  NM_166836.1  CG3114-RA, transcript variant A (ewg), mRNA 
0   0  0  3  3  NM_080111.2  CG2684-RA (lds), mRNA 
0   0  0  3  2  NM_135690.2  CG6746-RA (CG6746), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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