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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5561R-2 
 Symbol CG5561  Full Name CG5561 
 CG No CG5561  Old CG No CG5561 
 Synonyms CG5561 
 Accession No (Link to NCBI) NM_134752.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTACCGGAAGGCTCTCAAGGAA 
 Primer Seq. 3'
aatctagaggtaccATTATGACGGGCAGTCGAGCA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TACCGGAAGG CTCTCAAGGA ACTGGTCCGT TGTTACGATA AGAGGATACC AGACATTTTA 
0061 CATGTCCAAA GTGGACCTAT GACGATATCA GAGATGTCTG AACTTTTTTG CCGGAAGCTC 
0121 GATATTCCGA TGAGTTGGGA ATCGTTTCGG TATGAACTTA ACGAGCGAAC CTCCCATTTG 
0181 ATTGCAAATC CCCCGTTTAT GGATGGGATT GAGCGGCTTG TTCCGCACCT GCGCAATAGC 
0241 TGCATGGAAT TGGGCTTGAT CACCTCCAGC AACGAGGCCA ACTACTGCTC GAAGATCCGC 
0301 GGCCGGGAGG ACTTCTTTGA GAACTTCTCC ACCGTGGTCT GTGCGGATGA TCCGGAGTTG 
0361 CGGGCACCCA AACCCGAGCC CGACGTCTAC CTGATTGCCA TGTCGAGGCT AGGGGATGCT 
0421 GGACCTGATT GCACCCTGGT TTTCGATGGC ACTCCCAAAG GAGTTCAGGC GGCGAGCGAT 
0481 GCTCGACTGC CCGTCATAAT  
 in silico PCR Fragment
0001 TACCGGAAGG CTCTCAAGGA ACTGGTCCGT TGTTACGATA AGAGGATACC AGACATTTTA 
0061 CATGTCCAAA GTGGACCTAT GACGATATCA GAGATGTCTG AACTTTTTTG CCGGAAGCTC 
0121 GATATTCCGA TGAGTTGGGA ATCGTTTCGG TATGAACTTA ACGAGCGAAC CTCCCATTTG 
0181 ATTGCAAATC CCCCGTTTAT GGATGGGATT GAGCGGCTTG TTCCGCACCT GCGCAATAGC 
0241 TGCATGGAAT TGGGCTTGAT CACCTCCAGC AACGAGGCCA ACTACTGCTC GAAGATCCGC 
0301 GGCCGGGAGG ACTTCTTTGA GAACTTCTCC ACCGTGGTCT GTGCGGATGA TCCGGAGTTG 
0361 CGGGCACCCA AACCCGAGCC CGACGTCTAC CTGATTGCCA TGTCGAGGCT AGGGGATGCT 
0421 GGACCTGATT GCACCCTGGT TTTCGATGGC ACTCCCAAAG GAGTTCAGGC GGCGAGCGAT 
0481 GCTCGACTGC CCGTCATAAT  
 Assemble Data

5561R-2.IR_full       1   TACCGGAAGGCTCTCAAGGAACTGGTCCGTTGTTACGATAAGAGGATACCAGACATTTTA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     1   TACCGGAAGGCTCTCAAGGAACTGGTCCGTTGTTACGATAAGAGGATACCAGACATTTTA 60


5561R-2.IR_full       61  CATGTCCAAAGTGGACCTATGACGATATCAGAGATGTCTGAACTTTTTTGCCGGAAGCTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     61  CATGTCCAAAGTGGACCTATGACGATATCAGAGATGTCTGAACTTTTTTGCCGGAAGCTC 120


5561R-2.IR_full       121 GATATTCCGATGAGTTGGGAATCGTTTCGGTATGAACTTAACGAGCGAACCTCCCATTTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     121 GATATTCCGATGAGTTGGGAATCGTTTCGGTATGAACTTAACGAGCGAACCTCCCATTTG 180


5561R-2.IR_full       181 ATTGCAAATCCCCCGTTTATGGATGGGATTGAGCGGCTTGTTCCGCACCTGCGCAATAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     181 ATTGCAAATCCCCCGTTTATGGATGGGATTGAGCGGCTTGTTCCGCACCTGCGCAATAGC 240


5561R-2.IR_full       241 TGCATGGAATTGGGCTTGATCACCTCCAGCAACGAGGCCAACTACTGCTCGAAGATCCGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     241 TGCATGGAATTGGGCTTGATCACCTCCAGCAACGAGGCCAACTACTGCTCGAAGATCCGC 300


5561R-2.IR_full       301 GGCCGGGAGGACTTCTTTGAGAACTTCTCCACCGTGGTCTGTGCGGATGATCCGGAGTTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     301 GGCCGGGAGGACTTCTTTGAGAACTTCTCCACCGTGGTCTGTGCGGATGATCCGGAGTTG 360


5561R-2.IR_full       361 CGGGCACCCAAACCCGAGCCCGACGTCTACCTGATTGCCATGTCGAGGCTAGGGGATGCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     361 CGGGCACCCAAACCCGAGCCCGACGTCTACCTGATTGCCATGTCGAGGCTAGGGGATGCT 420


5561R-2.IR_full       421 GGACCTGATTGCACCCTGGTTTTCGATGGCACTCCCAAAGGAGTTCAGGCGGCGAGCGAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5561R-2.in silico     421 GGACCTGATTGCACCCTGGTTTTCGATGGCACTCCCAAAGGAGTTCAGGCGGCGAGCGAT 480


5561R-2.IR_full       481 GCTCGACTGCCCGTCATAAT 500
                          ||||||||||||||||||||
5561R-2.in silico     481 GCTCGACTGCCCGTCATAAT 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_134752.2  CG5561-RA (CG5561), mRNA 
2.28   11  12  17  NM_134751.1  CG5556-RA (CG5556), mRNA 
0   NM_142026.2  CG8863-RA, transcript variant A (CG8863), mRNA 
0   NM_169522.1  CG8863-RE, transcript variant E (CG8863), mRNA 
0   NM_169519.1  CG8863-RB, transcript variant B (CG8863), mRNA 
0   NM_169520.1  CG8863-RC, transcript variant C (CG8863), mRNA 
0   NM_169521.1  CG8863-RD, transcript variant D (CG8863), mRNA 
0   NM_057353.3  CG9668-RA (Rh4), mRNA 
0   NM_136567.2  CG8740-RA, transcript variant A (CG8740), mRNA 
0   NM_165626.1  CG8740-RB, transcript variant B (CG8740), mRNA 
0   NM_165627.1  CG8740-RC, transcript variant C (CG8740), mRNA 
0   NM_079746.4  CG5320-RA, transcript variant A (Gdh), mRNA 
0   NM_206551.1  CG5320-RF, transcript variant F (Gdh), mRNA 
0   NM_206550.1  CG5320-RE, transcript variant E (Gdh), mRNA 
0   NM_170101.1  CG5320-RB, transcript variant B (Gdh), mRNA 
0   NM_141939.2  CG5641-RA (CG5641), mRNA 
0   NM_168384.1  CG6718-RC, transcript variant C (CG6718), mRNA 
0   NM_140109.1  CG6718-RA, transcript variant A (CG6718), mRNA 
0   NM_168385.1  CG6718-RD, transcript variant D (CG6718), mRNA 
0   NM_168383.1  CG6718-RB, transcript variant B (CG6718), mRNA 
0   NM_137128.2  CG12864-RA, transcript variant A (Su(var)2-HP2), mRNA 
0   NM_078549.3  CG32688-RA, transcript variant A (Hk), mRNA 
0   NM_168706.1  CG3799-RC, transcript variant C (CG3799), mRNA 
0   NM_140685.4  CG3799-RA, transcript variant A (CG3799), mRNA 
0   NM_168705.1  CG3799-RB, transcript variant B (CG3799), mRNA 
0   NM_142552.2  CG6255-RA (CG6255), mRNA 
0   14  NM_175960.3  CG33196-RB (dp), mRNA 
0   13  NM_168725.2  CG13731-RA (CG13731), mRNA 
0   NM_134987.1  CG15431-RA (CG15431), mRNA 
0   NM_141196.1  CG9778-RA (CG9778), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
+- 
DC
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
DC
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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