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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5345R-2 |
| Symbol |
Eip55E |
Full Name |
Eip55E |
| CG No |
CG5345 |
Old CG No |
CG5345 |
| Synonyms |
EIP40, Eip40, Eip55BD, CG5345, Eip55E |
| Accession No (Link to NCBI) |
NM_137508.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGAGTACTCCCGCAGTGGAAA |
| Primer Seq. 3' |
aatctagaggtaccCACCCATGACCACATCCGTAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGAGTACTCC CGCAGTGGAA ATCCCACTAG GAACGTGCTG GAGACGTGCT TCgNCGCCTT
0061 GGATAATGCC AAATACGGCC TCACGTTCTC ATCGGGATTG GGAGCCACCA CCGCTGTGCT
0121 AACTATGCTG AGCAGCGGCG ANTCACATCA TCATGGGCGA CGATGTTTAC GGAGGCACCA
0181 ACCGTTTGAT CCGACAgGTT GCCACCCGTC TGGGAATCTC AGCCACCTTT GTGGATCCCA
0241 CGAAGTTGGA TCTAATTAAA AGTTCCATCA AGCCGGAGAC CAAGTTGGTG TGNGATCGAG
0301 TCGCCAACTA ATCCATTGGT AAAGGTAGCC GACATCGAGG CTATTGCGCA GCTGGTCCAT
0361 GGAGTACGCG AGGATATCGT CCTGGCCGTC GACAACAACC TTCCTGACCT CCTACTTCCA
0421 GCGACCCTTG GAgCTGGGCG CTGATCTGGT CTGCTACTCC CTGACCAAGT ACATGAACGG
0481 TCATACGGAT GTGGTCATGG GTG |
| in silico PCR Fragment |
0001 TGAGTACTCC CGCAGTGGAA ATCCCACTAG GAACGTGCTG GAGACGTGCT TCGCCGCCTT
0061 GGATAATGCC AAATACGGCC TCACGTTCTC ATCGGGATTG GGAGCCACCA CCGCTGTGCT
0121 AACTATGCTG AGCAGCGGCG A-TCACATCA TCATGGGCGA CGATGTTTAC GGAGGCACCA
0181 ACCGTTTGAT CCGACAGGTT GCCACCCGTC TGGGAATCTC AGCCACCTTT GTGGATCCCA
0241 CGAAGTTGGA TCTAATTAAA AGTTCCATCA AGCCGGAGAC CAAGTTGGTG TG-GATCGAG
0301 TCACCAACTA ATCCATTGGT AAAGGTAGCC GACATCGAGG CTATTGCGCA GCTGGTCCAT
0361 GGAGTACGCG AGGATATCGT CCTGGCCGTC GACAAC-ACC TTCCTGACCT CCTACTTCCA
0421 GCGACCCTTG GAGCTGGGCG CTGATCTGGT CTGCTACTCC CTGACCAAGT ACATGAACGG
0481 TCATACGGAT GTGGTCATGG GTG |
| Assemble Data |
5345R-2.IR_full 1 TGAGTACTCCCGCAGTGGAAATCCCACTAGGAACGTGCTGGAGACGTGCTTCGNCGCCTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
5345R-2.in silico 1 TGAGTACTCCCGCAGTGGAAATCCCACTAGGAACGTGCTGGAGACGTGCTTCGCCGCCTT 60
5345R-2.IR_full 61 GGATAATGCCAAATACGGCCTCACGTTCTCATCGGGATTGGGAGCCACCACCGCTGTGCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5345R-2.in silico 61 GGATAATGCCAAATACGGCCTCACGTTCTCATCGGGATTGGGAGCCACCACCGCTGTGCT 120
5345R-2.IR_full 121 AACTATGCTGAGCAGCGGCGANTCACATCATCATGGGCGACGATGTTTACGGAGGCACCA 180
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
5345R-2.in silico 121 AACTATGCTGAGCAGCGGCGA-TCACATCATCATGGGCGACGATGTTTACGGAGGCACCA 180
5345R-2.IR_full 181 ACCGTTTGATCCGACAGGTTGCCACCCGTCTGGGAATCTCAGCCACCTTTGTGGATCCCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5345R-2.in silico 181 ACCGTTTGATCCGACAGGTTGCCACCCGTCTGGGAATCTCAGCCACCTTTGTGGATCCCA 240
5345R-2.IR_full 241 CGAAGTTGGATCTAATTAAAAGTTCCATCAAGCCGGAGACCAAGTTGGTGTGNGATCGAG 300
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
5345R-2.in silico 241 CGAAGTTGGATCTAATTAAAAGTTCCATCAAGCCGGAGACCAAGTTGGTGTG-GATCGAG 300
5345R-2.IR_full 301 TCGCCAACTAATCCATTGGTAAAGGTAGCCGACATCGAGGCTATTGCGCAGCTGGTCCAT 360
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5345R-2.in silico 301 TCACCAACTAATCCATTGGTAAAGGTAGCCGACATCGAGGCTATTGCGCAGCTGGTCCAT 360
5345R-2.IR_full 361 GGAGTACGCGAGGATATCGTCCTGGCCGTCGACAACAACCTTCCTGACCTCCTACTTCCA 420
|||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
5345R-2.in silico 361 GGAGTACGCGAGGATATCGTCCTGGCCGTCGACAAC-ACCTTCCTGACCTCCTACTTCCA 420
5345R-2.IR_full 421 GCGACCCTTGGAGCTGGGCGCTGATCTGGTCTGCTACTCCCTGACCAAGTACATGAACGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5345R-2.in silico 421 GCGACCCTTGGAGCTGGGCGCTGATCTGGTCTGCTACTCCCTGACCAAGTACATGAACGG 480
5345R-2.IR_full 481 TCATACGGATGTGGTCATGGGTG 503
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5345R-2.in silico 481 TCATACGGATGTGGTCATGGGTG 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137508.2 |
CG5345-RA (Eip55E), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_138044.2 |
CG13569-RA, transcript variant A (CG13569), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_166663.1 |
CG13569-RB, transcript variant B (CG13569), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_135095.1 |
CG14014-RA (CG14014), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_143611.2 |
CG11334-RB, transcript variant B (CG11334), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_170551.1 |
CG11334-RA, transcript variant A (CG11334), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_142208.2 |
CG6125-RB, transcript variant B (CG6125), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169656.1 |
CG6125-RA, transcript variant A (CG6125), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169542.1 |
CG9374-RC, transcript variant C (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169545.1 |
CG9374-RF, transcript variant F (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169546.1 |
CG9374-RH, transcript variant H (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142045.2 |
CG9374-RB, transcript variant B (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169543.1 |
CG9374-RD, transcript variant D (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169544.1 |
CG9374-RE, transcript variant E (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169541.1 |
CG9374-RA, transcript variant A (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169547.1 |
CG9374-RI, transcript variant I (lkb1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080140.2 |
CG3923-RA (Exp6), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_079933.3 |
CG5408-RA (trbl), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_134890.2 |
CG17265-RA (CG17265), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165193.1 |
CG17932-RB, transcript variant B (Ugt36Bc), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_144370.2 |
CG17932-RA, transcript variant A (Ugt36Bc), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134545.2 |
CG15459-RA (CG15459), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_176242.1 |
CG33133-RA (grau), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142975.2 |
CG5669-RA (CG5669), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_136179.1 |
CG10721-RA (CG10721), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_141304.1 |
CG14673-RA (CG14673), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_137337.1 |
CG9640-RA (CG9640), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_079677.2 |
CG6027-RA (cdi), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_132804.1 |
CG9203-RA (CG9203), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_168449.1 |
CG32076-RA (Alg10), mRNA |
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