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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5323R-3

Symbol CG5323 Full Name CG5323

CG No CG5323 Old CG No CG5323

Synonyms CG5323

Accession No (Link to NCBI) NM_137502.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGATCCAGCAACAGCGCCAG

Primer Seq. 3'

aatctagaggtaccGACTTGGGGAATGCTCTCGGG

Predicted Fragment Size

IR fragment full Seq

0001 ATGATCCAGC AACAGCGCCA GAAAATCGAG GCCGCGGTAA CGGAGATGAT CGACGACATG 
0061 GACAAGGACG CACCTGCGCA AAATGCAGAA TGAGATGCAC TTGTGTGCGG CCAAGTGCTG 
0121 CCAGGATGGA ACCTCCAGCG TGGACAGTGT CCAGAGATGT GTGGATCGAT GCTCCGCGCC 
0181 CATGACGAGG GCACAGAACT ACGTTCAACA CGAATTGGGC GAGTTCCAGG GCAGACTTCA 
0241 ACGTTGCGTC ATGCAATGCA ACGACGATGT GAAGGTGAAA ATGCCGCCCA GTCCCAATGA 
0301 GGATCAGATA GCCAAGTACA CCGACCAATT CGAGCGCTGT GCCATCCAGT GTGTGGACAA 
0361 GCATGTGGGC CTCATTCCCG GCATGATGAA GACCATGAAG GCTGTGCTCT CCAAGGGACC 
0421 CGAGAGCATT CCCCAAGTC

in silico PCR Fragment

0001 ATGATCCAGC AACAGCGCCA GAAAATCGAG GCCGCGGTAA CGGAGATGAT CGACGACATG 
0061 GACAA-GACG CACCTGCGCA AAATGCAGAA TGAGATGCAC TTGTGTGCGG CCAAGTGCTG 
0121 CCAGGATGGA ACCTCCAGCG TGGACAGTGT CCAGAGATGT GTGGATCGAT GCTCCGCGCC 
0181 CATGACGAGG GCACAGAACT ACGTTCAACA CGAATTGGGC GAGTTCCAGG GCAGACTTCA 
0241 ACGTTGCGTC ATGCAATGCA ACGACGATGT GAAGGTGAAA ATGCCGCCCA GTCCCAATGA 
0301 GGATCAGATA GCCAAGTACA CCGACCAATT CGAGCGCTGT GCCATCCAGT GTGTGGACAA 
0361 GCATGTGGGC CTCATTCCCG GCATGATGAA GACCATGAAG GCTGTGCTCT CCAAGGGACC 
0421 CGAGAGCATT CCCCAAGTC

Assemble Data


5323R-3.IR_full       1   ATGATCCAGCAACAGCGCCAGAAAATCGAGGCCGCGGTAACGGAGATGATCGACGACATG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5323R-3.in silico     1   ATGATCCAGCAACAGCGCCAGAAAATCGAGGCCGCGGTAACGGAGATGATCGACGACATG 60


5323R-3.IR_full       61  GACAAGGACGCACCTGCGCAAAATGCAGAATGAGATGCACTTGTGTGCGGCCAAGTGCTG 120
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
5323R-3.in silico     61  GACAA-GACGCACCTGCGCAAAATGCAGAATGAGATGCACTTGTGTGCGGCCAAGTGCTG 120


5323R-3.IR_full       121 CCAGGATGGAACCTCCAGCGTGGACAGTGTCCAGAGATGTGTGGATCGATGCTCCGCGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5323R-3.in silico     121 CCAGGATGGAACCTCCAGCGTGGACAGTGTCCAGAGATGTGTGGATCGATGCTCCGCGCC 180


5323R-3.IR_full       181 CATGACGAGGGCACAGAACTACGTTCAACACGAATTGGGCGAGTTCCAGGGCAGACTTCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5323R-3.in silico     181 CATGACGAGGGCACAGAACTACGTTCAACACGAATTGGGCGAGTTCCAGGGCAGACTTCA 240


5323R-3.IR_full       241 ACGTTGCGTCATGCAATGCAACGACGATGTGAAGGTGAAAATGCCGCCCAGTCCCAATGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5323R-3.in silico     241 ACGTTGCGTCATGCAATGCAACGACGATGTGAAGGTGAAAATGCCGCCCAGTCCCAATGA 300


5323R-3.IR_full       301 GGATCAGATAGCCAAGTACACCGACCAATTCGAGCGCTGTGCCATCCAGTGTGTGGACAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5323R-3.in silico     301 GGATCAGATAGCCAAGTACACCGACCAATTCGAGCGCTGTGCCATCCAGTGTGTGGACAA 360


5323R-3.IR_full       361 GCATGTGGGCCTCATTCCCGGCATGATGAAGACCATGAAGGCTGTGCTCTCCAAGGGACC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5323R-3.in silico     361 GCATGTGGGCCTCATTCCCGGCATGATGAAGACCATGAAGGCTGTGCTCTCCAAGGGACC 420


5323R-3.IR_full       421 CGAGAGCATTCCCCAAGTC 439
                          |||||||||||||||||||
5323R-3.in silico     421 CGAGAGCATTCCCCAAGTC 439

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	420	0	0	0	NM_137502.3	CG5323-RA (CG5323), mRNA
0.47	2	2	2	4	NM_143283.1	CG5882-RA (CG5882), mRNA
0	0	2	4	5	NM_137701.2	CG4030-RA (CG4030), mRNA
0	0	2	3	5	NM_170360.1	CG4976-RA (Mes-4), mRNA
0	0	2	3	1	NM_206662.1	CG12065-RC, transcript variant C (CG12065), mRNA
0	0	2	3	1	NM_132265.2	CG12065-RA, transcript variant A (CG12065), mRNA
0	0	2	2	2	NM_137865.1	CG13518-RA (Obp58b), mRNA
0	0	1	3	3	NM_141805.1	CG5207-RA (scpr-A), mRNA
0	0	1	2	6	NM_078878.2	CG10123-RA (Top3alpha), mRNA
0	0	1	2	5	NM_136131.2	CG10189-RA (CG10189), mRNA
0	0	1	2	5	NM_142486.2	CG7708-RA, transcript variant A (CG7708), mRNA
0	0	1	2	5	NM_169832.1	CG7708-RB, transcript variant B (CG7708), mRNA
0	0	1	2	4	NM_134820.2	CG10882-RA (CG10882), mRNA
0	0	1	2	2	NM_001043138.1	CG17672-RA (CG17672), mRNA
0	0	0	6	4	NM_134708.2	CG13949-RA (CG13949), mRNA
0	0	0	5	10	NM_176591.1	CG15532-RC, transcript variant C (hdc), mRNA
0	0	0	5	10	NM_079853.2	CG15532-RA, transcript variant A (hdc), mRNA
0	0	0	5	7	NM_170478.1	CG15532-RB, transcript variant B (hdc), mRNA
0	0	0	5	6	NM_169964.1	CG31343-RA (CG31343), mRNA
0	0	0	5	3	NM_135169.2	CG9493-RA (Pez), mRNA
0	0	0	5	3	NM_080531.2	CG5481-RA (lea), mRNA
0	0	0	4	5	NM_169489.1	CG7518-RA, transcript variant A (CG7518), mRNA
0	0	0	4	5	NM_141993.2	CG7518-RB, transcript variant B (CG7518), mRNA
0	0	0	4	4	NM_143138.1	CG5127-RA (CG5127), mRNA
0	0	0	4	3	NM_080765.2	CG13777-RC, transcript variant C (milt), mRNA
0	0	0	4	3	NM_164736.1	CG13777-RA, transcript variant A (milt), mRNA
0	0	0	4	2	NM_164674.1	CG31643-RA (CG31643), mRNA
0	0	0	4	2	NM_141895.2	CG14740-RA (CG14740), mRNA
0	0	0	3	7	NM_137817.2	CG3413-RB, transcript variant B (wdp), mRNA
0	0	0	3	7	NM_166526.1	CG3413-RD, transcript variant D (wdp), mRNA

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