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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5156R-2 
 Symbol CG5156  Full Name CG5156 
 CG No CG5156  Old CG No CG5156 
 Synonyms CT16503, CG5156 
 Accession No (Link to NCBI) NM_134746.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTTCTACTGGCGCCCACCATTA 
 Primer Seq. 3'
aatctagaggtaccCAGCAGAAGGATCAGCAGGAC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TTCTACTGGC GCCCACCATT AATGCTTACG ATATCGTGGC CTgGGNTCAG ATGCCCTTCT 
0061 CCATGATCCT CCAGAGCGGC ACTACGTCTG TGGACACCTT CTTCCTGCTC AGTGGACTGC 
0121 TTCTGGTTCT GTCCGCCCTG CGTGAGATGG ATCGCTCCAA GGGTCGTCTG CATGTGCCAC 
0181 TGATGTATCT GCACCGTCTG GTTCGCCTGA CTCCTGTTTT GGCCCTGGCC GTGCTCATCT 
0241 TTATGACCCT TTTCCCCCGA CTGGACAGTG GTCCTCTGTG GAATCAGTTC ACCAGCTCAT 
0301 CGGANNTCTG NAGCGACACC TGGTGGGCAA CTCTGCTCTA CGTGNAAAAT TATNCTGCTC 
0361 CTGNTAGGAT GTgTCTGGGT CATTCGTGGT ATCTGGCTGT GGATATGCAA CTGTATATTA 
0421 TATCCCCACT CCTTCTGATC GCCCTTTACA AATGGGGCAA AAAGNCTNTC GCAGGAATTG 
0481 TCCTGCTGAT CCTTCTGCTG  
 in silico PCR Fragment
0001 TTCTACTGGC GCCCACCATT AATGCTTACG ATATCGTGGC CTGGGCTCAG ATGCCCTTCT 
0061 CCATGATCCT CCAGAGCGGC ACTACGTCTG TGGACACCTT CTTCCTGCTC AGTGGACTGC 
0121 TTCTGGTTCT GTCCGCCCTG CGTGAGATGG ATCGCTCCAA GGGTCGTCTG CATGTGCCAC 
0181 TGATGTATCT GCACCGTCTG GTTCGCCTGA CTCCTGTTTT GGCCCTGGCC GTGCTCATCT 
0241 TTATGACCCT TTTCCCCCGA CTGGACAGTG GTCCTCTGTG GAATCAGTTC ACCAGCTCAT 
0301 CGGAGCTCTG CAGCGACACC TGGTGGGCAA CTCTGCTCTA CGTGCAAAAT TATGCTGCTC 
0361 CTGGTAGGAT GTGTCTGGGT CATTCGTGGT ATCTGGCTGT GGATATGCAA CTGTATATTA 
0421 TATCCCCACT CCTTCTGATC GCCCTTTACA AATGGGGCAA AAAGGCTATC GCAGGAATTG 
0481 TCCTGCTGAT CCTTCTGCTG  
 Assemble Data

5156R-2.IR_full       1   TTCTACTGGCGCCCACCATTAATGCTTACGATATCGTGGCCTGGGNTCAGATGCCCTTCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
5156R-2.in silico     1   TTCTACTGGCGCCCACCATTAATGCTTACGATATCGTGGCCTGGGCTCAGATGCCCTTCT 60


5156R-2.IR_full       61  CCATGATCCTCCAGAGCGGCACTACGTCTGTGGACACCTTCTTCCTGCTCAGTGGACTGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5156R-2.in silico     61  CCATGATCCTCCAGAGCGGCACTACGTCTGTGGACACCTTCTTCCTGCTCAGTGGACTGC 120


5156R-2.IR_full       121 TTCTGGTTCTGTCCGCCCTGCGTGAGATGGATCGCTCCAAGGGTCGTCTGCATGTGCCAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5156R-2.in silico     121 TTCTGGTTCTGTCCGCCCTGCGTGAGATGGATCGCTCCAAGGGTCGTCTGCATGTGCCAC 180


5156R-2.IR_full       181 TGATGTATCTGCACCGTCTGGTTCGCCTGACTCCTGTTTTGGCCCTGGCCGTGCTCATCT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5156R-2.in silico     181 TGATGTATCTGCACCGTCTGGTTCGCCTGACTCCTGTTTTGGCCCTGGCCGTGCTCATCT 240


5156R-2.IR_full       241 TTATGACCCTTTTCCCCCGACTGGACAGTGGTCCTCTGTGGAATCAGTTCACCAGCTCAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5156R-2.in silico     241 TTATGACCCTTTTCCCCCGACTGGACAGTGGTCCTCTGTGGAATCAGTTCACCAGCTCAT 300


5156R-2.IR_full       301 CGGANNTCTGNAGCGACACCTGGTGGGCAACTCTGCTCTACGTGNAAAATTATNCTGCTC 360
                          ||||  |||| ||||||||||||||||||||||||||||||||| |||||||| ||||||
5156R-2.in silico     301 CGGAGCTCTGCAGCGACACCTGGTGGGCAACTCTGCTCTACGTGCAAAATTATGCTGCTC 360


5156R-2.IR_full       361 CTGNTAGGATGTGTCTGGGTCATTCGTGGTATCTGGCTGTGGATATGCAACTGTATATTA 420
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5156R-2.in silico     361 CTGGTAGGATGTGTCTGGGTCATTCGTGGTATCTGGCTGTGGATATGCAACTGTATATTA 420


5156R-2.IR_full       421 TATCCCCACTCCTTCTGATCGCCCTTTACAAATGGGGCAAAAAGNCTNTCGCAGGAATTG 480
                          |||||||||||||||||||||||||||||||||||||||||||| || ||||||||||||
5156R-2.in silico     421 TATCCCCACTCCTTCTGATCGCCCTTTACAAATGGGGCAAAAAGGCTATCGCAGGAATTG 480


5156R-2.IR_full       481 TCCTGCTGATCCTTCTGCTG 500
                          ||||||||||||||||||||
5156R-2.in silico     481 TCCTGCTGATCCTTCTGCTG 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_134746.1  CG5156-RA (CG5156), mRNA 
0   29  26  NM_134477.1  CG14204-RA (CG14204), mRNA 
0   14  NM_142903.2  CG10183-RA (CG10183), mRNA 
0   11  22  NM_132349.2  CG3106-RA (CG3106), mRNA 
0   NM_142839.1  CG4725-RA (CG4725), mRNA 
0   NM_142942.3  CG31140-RA, transcript variant A (CG31140), mRNA 
0   NM_206553.1  CG31140-RB, transcript variant B (CG31140), mRNA 
0   NM_206552.1  CG31140-RC, transcript variant C (CG31140), mRNA 
0   NM_135278.1  CG6055-RA (CG6055), mRNA 
0   NM_168667.1  CG32159-RB (CG32159), mRNA 
0   NM_169682.1  CG31291-RB, transcript variant B (CG31291), mRNA 
0   NM_169683.1  CG31291-RA, transcript variant A (CG31291), mRNA 
0   12  11  NM_136992.2  CG13325-RA (CG13325), mRNA 
0   NM_136741.2  CG11763-RC, transcript variant C (micr), mRNA 
0   NM_165770.1  CG11763-RA, transcript variant A (micr), mRNA 
0   NM_001038849.1  CG11763-RD, transcript variant D (micr), mRNA 
0   NM_206013.2  CG33321-RA (CheB38b), mRNA 
0   NM_164401.3  CG31795-RB, transcript variant B (ia2), mRNA 
0   NM_134718.4  CG31795-RA, transcript variant A (ia2), mRNA 
0   NM_130670.1  CG2709-RA (CG2709), mRNA 
0   NM_132457.1  CG11122-RA (CG11122), mRNA 
0   NM_135394.2  CG13089-RA (CG13089), mRNA 
0   NM_001038882.1  CG4622-RB, transcript variant B (CG4622), mRNA 
0   NM_138086.2  CG11413-RA (CG11413), mRNA 
0   NM_132267.4  CG12109-RB (Caf1-180), mRNA 
0   NM_143176.2  CG14354-RA (CG14354), mRNA 
0   NM_079111.2  CG2827-RA (Tal), mRNA 
0   NM_167312.1  CG10353-RB, transcript variant B (CG10353), mRNA 
0   NM_165693.2  CG30004-RA (CG30004), mRNA 
0   11  NM_206546.1  CG33337-RA (CG33337), mRNA 

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