NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4998R-2

Symbol

CG4998

Full Name

CG4998

CG No

CG4998

Old CG No

CG4998

Synonyms

SPH66, CG4998

Accession No (Link to NCBI)

NM_140621.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.<br> Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.<br> Curr Biol (2006) 16(8) 808-13 [ PubMed ID = 16631589 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCAGTCGCCGATCTGTCGATA

Primer Seq. 3'

aatctagaggtaccTGGCGGTCCAAAGGAGTTCAC

Predicted Fragment Size

IR fragment full Seq

0001 CCAGTCGCCG ATCTGTCGAT ACCAAGCCCG CCGAACAAGC TGACATTCAA GGACGTTACC 
0061 TCGTCCACGA GTCCATCCAG AGCAACAGCA CCACCCAGAT TGACGAGGTC ATCGAGCAGC 
0121 TGATCAACTC CCGCCGTGAG GGTCGTAATC TGGGGGAATA CGATGCCGTC TATGCTGATG 
0181 GCAATATCGA TCAGGCTCTG CAGCAGGGAA ACGATATGCA GGCCCGCAAT CTTATCCGGG 
0241 ATAAGCTGTG TGGATTGGGA CTGATGAGCT GCGATGTGGA GGAGAAGCGT CCCTTCTACT 
0301 CGACCATCTA TGCCCAGGGT CCCCCACCAC CATCCGGTTT CAGCGGTGGT CCCTATGGTC 
0361 CGGCTAAGCC CATGCCCCCA CCAAGCTACT TCAGTGGNTC GTCCACCAAT GGGTGGACCT 
0421 CCTGGATCTT ACGGACCTCC GCCAAGCTCC CTGAACTCCT TTGGACCTCC GCCCAGCTCC 
0481 GTGAACTCCT TTGGACCGCC G

in silico PCR Fragment

0001 CCAGTCGCCG ATCTGTCGAT ACCAAGCCCG CCGAACAAGC TGACATTCAG GGACGTTACC 
0061 TCGTCCACGA GTCCATCCAG AGCAACAGCA CCACCCAGAT TGACGAGGTC ATCGAGCAGC 
0121 TGATCAACTC CCGCCGTGAG GGTCGTAATC TGGGGGAATA CGATGCCGTC TATGCTGATG 
0181 GCAATATCGA TCAGGCTCTG CAGCAGGGAA ACGATATGCA GGCCCGCAAT CTTATCCGGG 
0241 ATAAGCTGTG TGGATTGGGA CTGATGAGCT GCGATGTGGA GGAGAAGCGT CCCTTCTACT 
0301 CGACCATCTA TGCCCAGGGT CCCCCACCAC CATCTGGTTT CAGCGGTGGT CCCTATGGTC 
0361 CGGCTAAGCC CATGCCCCCA CCAAGCTACT TCAGTGG-TC GTCCACCAAT GGGTGGACCT 
0421 CCTGGATCTT ACGGACCTCC GCCAAGCTCC CTGAACTCCT TTGGACCTCC GCCCAGCTCC 
0481 GTGAACTCCT TTGGACCGCC A

Assemble Data

<PRE>

4998R-2.IR_full       1   CCAGTCGCCGATCTGTCGATACCAAGCCCGCCGAACAAGCTGACATTCA<span class="snp"><tt>A</tt></span>GGACGTTACC 60
                          |||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||
4998R-2.in silico     1   CCAGTCGCCGATCTGTCGATACCAAGCCCGCCGAACAAGCTGACATTCA<span class="snp"><tt>G</tt></span>GGACGTTACC 60


4998R-2.IR_full       61  TCGTCCACGAGTCCATCCAGAGCAACAGCACCACCCAGATTGACGAGGTCATCGAGCAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4998R-2.in silico     61  TCGTCCACGAGTCCATCCAGAGCAACAGCACCACCCAGATTGACGAGGTCATCGAGCAGC 120


4998R-2.IR_full       121 TGATCAACTCCCGCCGTGAGGGTCGTAATCTGGGGGAATACGATGCCGTCTATGCTGATG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4998R-2.in silico     121 TGATCAACTCCCGCCGTGAGGGTCGTAATCTGGGGGAATACGATGCCGTCTATGCTGATG 180


4998R-2.IR_full       181 GCAATATCGATCAGGCTCTGCAGCAGGGAAACGATATGCAGGCCCGCAATCTTATCCGGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4998R-2.in silico     181 GCAATATCGATCAGGCTCTGCAGCAGGGAAACGATATGCAGGCCCGCAATCTTATCCGGG 240


4998R-2.IR_full       241 ATAAGCTGTGTGGATTGGGACTGATGAGCTGCGATGTGGAGGAGAAGCGTCCCTTCTACT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4998R-2.in silico     241 ATAAGCTGTGTGGATTGGGACTGATGAGCTGCGATGTGGAGGAGAAGCGTCCCTTCTACT 300


4998R-2.IR_full       301 CGACCATCTATGCCCAGGGTCCCCCACCACCATC<span class="snp"><tt>C</tt></span>GGTTTCAGCGGTGGTCCCTATGGTC 360
                          ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||
4998R-2.in silico     301 CGACCATCTATGCCCAGGGTCCCCCACCACCATC<span class="snp"><tt>T</tt></span>GGTTTCAGCGGTGGTCCCTATGGTC 360


4998R-2.IR_full       361 CGGCTAAGCCCATGCCCCCACCAAGCTACTTCAGTGG<span class="snp"><tt>N</tt></span>TCGTCCACCAATGGGTGGACCT 420
                          |||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
4998R-2.in silico     361 CGGCTAAGCCCATGCCCCCACCAAGCTACTTCAGTGG<span class="snp"><tt>-</tt></span>TCGTCCACCAATGGGTGGACCT 420


4998R-2.IR_full       421 CCTGGATCTTACGGACCTCCGCCAAGCTCCCTGAACTCCTTTGGACCTCCGCCCAGCTCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4998R-2.in silico     421 CCTGGATCTTACGGACCTCCGCCAAGCTCCCTGAACTCCTTTGGACCTCCGCCCAGCTCC 480


4998R-2.IR_full       481 GTGAACTCCTTTGGACCGCC<span class="snp"><tt>G</tt></span> 501
                          ||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
4998R-2.in silico     481 GTGAACTCCTTTGGACCGCC<span class="snp"><tt>A</tt></span> 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	12	32	9	NM_001043144.1	CG4998-RB, transcript variant B (CG4998), mRNA
100	482	12	32	9	NM_140621.1	CG4998-RA, transcript variant A (CG4998), mRNA
0	0	3	2	3	NM_001031886.1	CG33692-RA, transcript variant A (CG33692), mRNA
0	0	3	2	3	NM_001031889.1	CG33691-RA, transcript variant A (CG33691), mRNA
0	0	3	2	3	NM_001031887.1	CG33691-RC, transcript variant C (CG33691), mRNA
0	0	2	5	2	NM_130587.1	CG14799-RA (CG14799), mRNA
0	0	2	4	5	NM_168124.1	CG10596-RC, transcript variant C (Msr-110), mRNA
0	0	2	4	5	NM_168123.1	CG10596-RA, transcript variant A (Msr-110), mRNA
0	0	2	4	5	NM_139716.2	CG10596-RB, transcript variant B (Msr-110), mRNA
0	0	2	3	2	NM_130646.2	CG14045-RA (CG14045), mRNA
0	0	2	2	4	NM_137785.1	CG9308-RA (CG9308), mRNA
0	0	1	3	5	NM_137287.1	CG15712-RA (CG15712), mRNA
0	0	1	3	4	NM_167431.1	CG15028-RA, transcript variant A (CG15028), mRNA
0	0	1	3	4	NM_132779.1	CG15028-RB, transcript variant B (CG15028), mRNA
0	0	1	3	4	NM_140438.2	CG32139-RA (Sox21b), mRNA
0	0	1	3	2	NM_078981.2	CG8967-RA (otk), mRNA
0	0	1	2	5	NM_136675.1	CG1625-RA (CG1625), mRNA
0	0	1	2	5	NM_167643.1	CG32537-RA (CG32537), mRNA
0	0	1	2	5	NM_136727.2	CG18408-RA, transcript variant A (CAP), mRNA
0	0	1	2	4	NM_136073.2	CG18397-RA (CG18397), mRNA
0	0	1	2	3	NM_080333.2	CG3346-RA (pon), mRNA
0	0	0	9	4	NM_057949.3	CG10270-RA (D19B), mRNA
0	0	0	6	6	NM_079817.2	CG1842-RA (Dhc98D), mRNA
0	0	0	6	3	NM_079470.2	CG10564-RA (Ac78C), mRNA
0	0	0	5	6	NM_168008.1	CG17746-RB, transcript variant B (CG17746), mRNA
0	0	0	5	6	NM_139537.2	CG17746-RA, transcript variant A (CG17746), mRNA
0	0	0	5	3	NM_166823.1	CG11059-RB, transcript variant B (cals), mRNA
0	0	0	5	3	NM_079893.2	CG11059-RA, transcript variant A (cals), mRNA
0	0	0	5	3	NM_137831.2	CG4752-RA (CG4752), mRNA
0	0	0	4	8	NM_139464.2	CG32306-RB, transcript variant B (CG32306), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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