NIG-Fly - Fly Stocks of National Institute of Genetics -

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4994R-1

Symbol

Mpcp

Full Name

Mitochondrial phosphate carrier protein

CG No

CG4994

Old CG No

CG4994

Synonyms

CG4994, MPCP, PpsM, l(3)00564, Mpcp

Accession No (Link to NCBI)

NM_079345.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATCTGGTGAAGTGCCGTCTG

Primer Seq. 3'

aatctagaggtaccGGCTTGGGCACCACATACTTG

Predicted Fragment Size

IR fragment full Seq

0001 GATCTGGTGA AGTGCCGTCT GCAGGTCGAC CAGGCCAAGT ACAAGAATCT GGTGCACGGA 
0061 TTCAAGGTCA CCGTGGCGGA GGAGGGCGCC CGCGGACTGG CTAAGGGCTG GTTCCCCACT 
0121 CTGCTCGGCT ACTCGGCACA GGGTCTGTGC AAGTTCGGTC TGTACGAGTT GTTCAAGGTG 
0181 AAGTACGCCG AAATCATTGG CGAGGAGAAC GCCTACCTGT ACCGCACCTC CTTGTATCTG 
0241 GCTGCTTCCG CTTCGGCCGA GTTCTTCGCC GATATCGCTC TGGCGCCGTT CGAGGCCGCC 
0301 AAGGTGAAGA TCCAGACTAT TCCTGGATAC GCCAACAACT TCCGCGAgGC AGTGCCCAAG 
0361 ATGCTANAAG GAGGAGGGCG TCAATGCCTT CTACAAGGGT CTGGTTCCCC TGTGGATGCG 
0421 ACAGATCCCA TACACCATGA TGAAGTTCGC TTGCTTCGAG CGCACCGTGG AGCTGCTCTA 
0481 CAAGTATGTG GTGCCCANNN C

in silico PCR Fragment

0001 GATCTGGTGA AGTGCCGTCT GCAGGTCGAC CAGGCCAAGT ACAAGAATCT GGTGCACGGA 
0061 TTCAAGGTCA CCGTGGCGGA GGAGGGCGCC CGCGGACTGG CTAAGGGCTG GTTCCCCACT 
0121 CTGCTCGGCT ACTCGGCACA GGGTCTGTGC AAGTTCGGTC TGTACGAGTT GTTCAAGGTG 
0181 AAGTACGCCG AAATCATTGG CGAGGAGAAC GCCTACCTGT ACCGCACCTC CTTGTATCTG 
0241 GCTGCTTCCG CTTCGGCCGA GTTCTTCGCC GATATCGCTC TGGCGCCGTT CGAGGCCGCC 
0301 AAGGTGAAGA TCCAGACTAT TCCCGGATAC GCCAACAACT TCCGCGAGGC AGTGCCCAAG 
0361 ATGCTA-AAG GAGGAGGGCG TCAATGCCTT CTACAAGGGT CTGGTTCCCC TGTGGATGCG 
0421 ACAGATCCCA TACACCATGA TGAAGTTCGC TTGCTTCGAG CGCACCGTGG AGCTGCTCTA 
0481 CAAGTATGTG GTGCCCAAGC C

Assemble Data

<PRE>

4994R-1.IR_full       1   GATCTGGTGAAGTGCCGTCTGCAGGTCGACCAGGCCAAGTACAAGAATCTGGTGCACGGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4994R-1.in silico     1   GATCTGGTGAAGTGCCGTCTGCAGGTCGACCAGGCCAAGTACAAGAATCTGGTGCACGGA 60


4994R-1.IR_full       61  TTCAAGGTCACCGTGGCGGAGGAGGGCGCCCGCGGACTGGCTAAGGGCTGGTTCCCCACT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4994R-1.in silico     61  TTCAAGGTCACCGTGGCGGAGGAGGGCGCCCGCGGACTGGCTAAGGGCTGGTTCCCCACT 120


4994R-1.IR_full       121 CTGCTCGGCTACTCGGCACAGGGTCTGTGCAAGTTCGGTCTGTACGAGTTGTTCAAGGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4994R-1.in silico     121 CTGCTCGGCTACTCGGCACAGGGTCTGTGCAAGTTCGGTCTGTACGAGTTGTTCAAGGTG 180


4994R-1.IR_full       181 AAGTACGCCGAAATCATTGGCGAGGAGAACGCCTACCTGTACCGCACCTCCTTGTATCTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4994R-1.in silico     181 AAGTACGCCGAAATCATTGGCGAGGAGAACGCCTACCTGTACCGCACCTCCTTGTATCTG 240


4994R-1.IR_full       241 GCTGCTTCCGCTTCGGCCGAGTTCTTCGCCGATATCGCTCTGGCGCCGTTCGAGGCCGCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4994R-1.in silico     241 GCTGCTTCCGCTTCGGCCGAGTTCTTCGCCGATATCGCTCTGGCGCCGTTCGAGGCCGCC 300


4994R-1.IR_full       301 AAGGTGAAGATCCAGACTATTCC<span class="snp"><tt>T</tt></span>GGATACGCCAACAACTTCCGCGAGGCAGTGCCCAAG 360
                          |||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||
4994R-1.in silico     301 AAGGTGAAGATCCAGACTATTCC<span class="snp"><tt>C</tt></span>GGATACGCCAACAACTTCCGCGAGGCAGTGCCCAAG 360


4994R-1.IR_full       361 ATGCTA<span class="snp"><tt>N</tt></span>AAGGAGGAGGGCGTCAATGCCTTCTACAAGGGTCTGGTTCCCCTGTGGATGCG 420
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
4994R-1.in silico     361 ATGCTA<span class="snp"><tt>-</tt></span>AAGGAGGAGGGCGTCAATGCCTTCTACAAGGGTCTGGTTCCCCTGTGGATGCG 420


4994R-1.IR_full       421 ACAGATCCCATACACCATGATGAAGTTCGCTTGCTTCGAGCGCACCGTGGAGCTGCTCTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4994R-1.in silico     421 ACAGATCCCATACACCATGATGAAGTTCGCTTGCTTCGAGCGCACCGTGGAGCTGCTCTA 480


4994R-1.IR_full       481 CAAGTATGTGGTGCCCA<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>C 501
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|
4994R-1.in silico     481 CAAGTATGTGGTGCCCA<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>C 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079345.2	CG4994-RA, transcript variant A (Mpcp), mRNA
100	482	0	0	0	NM_168588.1	CG4994-RB, transcript variant B (Mpcp), mRNA
4.14	20	62	58	102	NM_137624.1	CG9090-RA (CG9090), mRNA
0	0	4	3	3	NM_169549.1	CG9351-RC, transcript variant C (flfl), mRNA
0	0	4	3	3	NM_142047.1	CG9351-RA, transcript variant A (flfl), mRNA
0	0	4	3	3	NM_169548.1	CG9351-RB, transcript variant B (flfl), mRNA
0	0	3	5	4	NM_079258.2	CG5939-RA, transcript variant A (Prm), mRNA
0	0	3	5	4	NM_168291.1	CG5939-RC, transcript variant C (Prm), mRNA
0	0	3	5	4	NM_168290.1	CG5939-RB, transcript variant B (Prm), mRNA
0	0	3	5	4	NM_168292.1	CG5939-RD, transcript variant D (Prm), mRNA
0	0	3	2	2	NM_167776.1	CG14619-RE, transcript variant E (CG14619), mRNA
0	0	3	2	2	NM_167775.1	CG14619-RD, transcript variant D (CG14619), mRNA
0	0	3	2	2	NM_134618.2	CG14619-RA, transcript variant A (CG14619), mRNA
0	0	2	5	4	NM_141032.2	CG10512-RA, transcript variant A (CG10512), mRNA
0	0	2	5	4	NM_206411.1	CG10512-RD, transcript variant D (CG10512), mRNA
0	0	2	5	4	NM_168889.1	CG10512-RB, transcript variant B (CG10512), mRNA
0	0	2	5	4	NM_168890.1	CG10512-RC, transcript variant C (CG10512), mRNA
0	0	2	5	3	NM_080257.2	CG6815-RA (bor), mRNA
0	0	2	4	4	NM_142220.1	CG6045-RA (CG6045), mRNA
0	0	2	2	7	NM_130550.2	CG11417-RA (CG11417), mRNA
0	0	1	5	5	NM_079821.2	CG1954-RA (Pkc98E), mRNA
0	0	1	4	4	NM_079282.1	CG3322-RA (LanB2), mRNA
0	0	1	3	8	NM_170004.1	CG7070-RB, transcript variant B (PyK), mRNA
0	0	1	3	8	NM_079724.2	CG7070-RA, transcript variant A (PyK), mRNA
0	0	1	3	7	NM_133143.1	CG8002-RA (rictor), mRNA
0	0	1	3	2	NM_139859.1	CG8563-RA (CG8563), mRNA
0	0	1	3	2	NM_130657.2	CG13762-RA (CG13762), mRNA
0	0	1	2	8	NM_206421.1	CG33292-RA (CG33292), mRNA
0	0	1	2	3	NM_138180.1	CG13881-RA (CG13881), mRNA
0	0	1	2	3	NM_132544.1	CG18130-RA (CG18130), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

Back