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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4974R-3

Symbol

dally

Full Name

division abnormally delayed

CG No

CG4974

Old CG No

CG4974

Synonyms

CG4974, CT15952, gem, Dally, clone 1.63, l(3)06464, anon-EST:Liang-1.63, dally

Accession No (Link to NCBI)

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGACCTGGATGGTATCCACCAC

Primer Seq. 3'

aatctagaggtaccCCCAGTTGGCCATTGCTGTTC

Predicted Fragment Size

IR fragment full Seq

0001 AGACGTCGCC TACAGCGGGA CTCGCGGNCG AAGGACGCGT TGGGGGATCG ACCCACCAGT 
0061 GTGATGCCGT CAAGAGCTAC TTCGAATCGA TCGACATCAA GTCGAGTGGG ACTTACAGCG 
0121 AAAAAGGCGC CATTTGTGGC GGAAACTGCT GCAACAATGC CACGGAGCTG GAGCTGCGGG 
0181 ACAAAGCCGC CGGAATGTTC GAGCAACTGC TTCATCATCA CACCAGCAGC CTGCGCGGAG 
0241 TCCTCGAGAC GAATGCCAAA CAGTTCCAAA GTCACGTCCT GGAATTGGCG CAGATTTCGG 
0301 AGAACATGAC CCACTCGCTC TTTTCGAAGG TTTATACCCG TATGGTGCCT TCATCGCGTA 
0361 TGATGATCCA TCAACTGTAC ACCGAAATCA TGAACCATCT GATTTACACC TCCAACTATA 
0421 CGAACAGCAA TGGCCAACTG GG

in silico PCR Fragment

0001 gacctggatg gtatccacca ccaccagcac cacctgcaca gcgccaccac gcaccacaga 
0061 cgtcgcctac agcgggactc gcgggcgaag gacgccgttg ggggatcgac ccaccagtgt 
0121 gatgccgtca agagctactt cgaatcgatc gacatcaagt cgagtgggac ttacagcgaa 
0181 aaaggcgcca tttgtggcgg aaactgctgc aacaatgcca cggagctgga gctgcgggac 
0241 aaagccgccg gaatgttcga gcaactgctt catcatcaca ccagcagcct gcgcggagtc 
0301 ctcgagacga atgccaaaca gttccaaagt cacgtcctgg aattggcgca gatttcggag 
0361 aacatgaccc actcgctctt ttcgaaggtt tatacccgta tggtgccttc atcgcgtatg 
0421 atgatccatc aactgtacac cgaaatcatg aaccatctga tttacacctc caactatacg 
0481 aacagcaatg gccaactggg

Assemble Data

<PRE>

4974R-3.IR full       1   <span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>AGA 60
                          <span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||
4974R-3.in silico     1   <span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span>AGA 60


4974R-3.IR full       61  CGTCGCCTACAGCGGGACTCGCGG<span class="snp"><tt>N</tt></span>CGAAGGACGC<span class="snp"><tt>-</tt></span>GTTGGGGGATCGACCCACCAGTGT 120
                          ||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
4974R-3.in silico     61  CGTCGCCTACAGCGGGACTCGCGG<span class="snp"><tt>G</tt></span>CGAAGGACGC<span class="snp"><tt>C</tt></span>GTTGGGGGATCGACCCACCAGTGT 120


4974R-3.IR full       121 GATGCCGTCAAGAGCTACTTCGAATCGATCGACATCAAGTCGAGTGGGACTTACAGCGAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-3.in silico     121 GATGCCGTCAAGAGCTACTTCGAATCGATCGACATCAAGTCGAGTGGGACTTACAGCGAA 180


4974R-3.IR full       181 AAAGGCGCCATTTGTGGCGGAAACTGCTGCAACAATGCCACGGAGCTGGAGCTGCGGGAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-3.in silico     181 AAAGGCGCCATTTGTGGCGGAAACTGCTGCAACAATGCCACGGAGCTGGAGCTGCGGGAC 240


4974R-3.IR full       241 AAAGCCGCCGGAATGTTCGAGCAACTGCTTCATCATCACACCAGCAGCCTGCGCGGAGTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-3.in silico     241 AAAGCCGCCGGAATGTTCGAGCAACTGCTTCATCATCACACCAGCAGCCTGCGCGGAGTC 300


4974R-3.IR full       301 CTCGAGACGAATGCCAAACAGTTCCAAAGTCACGTCCTGGAATTGGCGCAGATTTCGGAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-3.in silico     301 CTCGAGACGAATGCCAAACAGTTCCAAAGTCACGTCCTGGAATTGGCGCAGATTTCGGAG 360


4974R-3.IR full       361 AACATGACCCACTCGCTCTTTTCGAAGGTTTATACCCGTATGGTGCCTTCATCGCGTATG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-3.in silico     361 AACATGACCCACTCGCTCTTTTCGAAGGTTTATACCCGTATGGTGCCTTCATCGCGTATG 420


4974R-3.IR full       421 ATGATCCATCAACTGTACACCGAAATCATGAACCATCTGATTTACACCTCCAACTATACG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-3.in silico     421 ATGATCCATCAACTGTACACCGAAATCATGAACCATCTGATTTACACCTCCAACTATACG 480


4974R-3.IR full       481 AACAGCAATGGCCAACTGGG 500
                          ||||||||||||||||||||
4974R-3.in silico     481 AACAGCAATGGCCAACTGGG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079259.2	division abnormally delayed CG4974-RA (dally), mRNA
0.82	4	5	2	6	NM_079321.2	araucan CG10571-RA (ara), mRNA
0.82	4	4	6	19	NM_143061.1	tenectin CG13648-RA (tnc), mRNA
0.62	3	12	29	52	NM_143342.1	CG5514-RB, transcript variant B (CG5514), mRNA
0.62	3	12	29	52	NM_170361.1	CG5514-RA, transcript variant A (CG5514), mRNA
0.41	2	2	17	18	NM_057805.2	toucan CG9660-RA, transcript variant A (toc), mRNA
0.41	2	2	14	15	NM_176229.1	CG33147-RA (CG33147), mRNA
0.41	2	2	5	12	NM_135286.1	CG6739-RA (CG6739), mRNA
0.2	1	5	13	21	NM_080362.2	SAM-motif ubiquitously expressed punctatedly localized protein CG31868-RA (Samuel), mRNA
0.2	1	4	8	20	NM_132370.1	CG2989-RA (CG2989), mRNA
0.2	1	3	6	48	NM_134527.1	CG15322-RA (CG15322), mRNA
0.2	1	2	9	30	NM_132004.2	CG4136-RA (CG4136), mRNA
0.2	1	2	5	13	NM_132498.1	CG1572-RA, transcript variant A (CG1572), mRNA
0.2	1	2	5	13	NM_167289.1	CG1572-RB, transcript variant B (CG1572), mRNA
0.2	1	2	5	10	NM_057980.3	Accessory gland-specific peptide 32CD CG4605-RA (Acp32CD), mRNA
0.2	1	2	5	9	NM_167023.1	CG32770-RA (CG32770), mRNA
0	0	10	12	37	NM_132391.2	CG15307-RA (CG15307), mRNA
0	0	9	29	38	NM_140417.2	CG17364-RA, transcript variant A (CG17364), mRNA
0	0	9	29	38	NM_176328.1	CG17364-RB, transcript variant B (CG17364), mRNA
0	0	9	19	47	NM_137672.1	CG15225-RA (CG15225), mRNA
0	0	9	15	33	NM_132214.1	CG15335-RB (CG15335), mRNA
0	0	9	9	28	NM_143571.2	CG15544-RA (CG15544), mRNA
0	0	8	9	24	NM_078544.2	lozenge CG1689-RA (lz), mRNA
0	0	7	14	47	NM_134552.3	RhoGAP19D CG1412-RA (RhoGAP19D), mRNA
0	0	7	13	34	NM_169047.1	l(3)82Fd CG32464-RD, transcript variant D (l(3)82Fd), mRNA
0	0	7	11	29	NM_132009.2	CG11473-RA (CG11473), mRNA
0	0	7	9	27	NM_206663.1	CG12075-RB, transcript variant B (CG12075), mRNA
0	0	7	9	27	NM_132278.2	CG12075-RA, transcript variant A (CG12075), mRNA
0	0	7	9	21	NM_143633.1	CG2187-RA (CG2187), mRNA
0	0	7	8	33	NM_080120.2	male-specific opa containing gene CG14560-RA (msopa), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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