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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4935R-1

Symbol CG4935 Full Name CG4935

CG No CG4935 Old CG No CG4935

Synonyms MET30, BG:DS02740.2, CG4935

Accession No (Link to NCBI) NM_135938.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAATCCGGCATCGGGATTACT

Primer Seq. 3'

aatctagaggtaccCCAGCAGACGAACCACACTAT

Predicted Fragment Size

IR fragment full Seq

0001 GAATCCGGCA TCGGGATTAC TGCTTAAGAC TTATGGTGGT CATGCGGATG AAGTCACCGA 
0061 TGCGGCGGGA AGCTGTGATA GTAGCTATAT CGTTTCCGCC AGCTTGGATA AAAGCATTAT 
0121 CTACTGGGAC GTGTCAACGG GAGCACCAGT ACGGCGCCTG CGTAGCCATG CCGGAGGTGT 
0181 TAGATGCGTC TGCTTCAACG AGGACTCCTC GATAGCCATT TCCGGGGGTC GAGATAATGC 
0241 CGTAATGTGC TGGGACATTC GAACGCGTCG CCTGGATCCC GTTCAGGTGA TGAAGGAGGC 
0301 CCGGGACTGC ATCACCACGG TGGCAACCAA CGAGAATCGC ATCTACGCCG CCTCCTTGGA 
0361 CGGATGTGTG AGGACCTACG ATATTCGGGT GGGCGAACTT ACCTGCGACA AAATTGGTGA 
0421 ACCCATCACA TATNCTGGCG CAGACGCGCG ACGAGCAATG TCTGGTGGCC GGCTGTCAGG 
0481 ATAGTGTGGT TCGTCTGCTG G

in silico PCR Fragment

0001 GAATCCGGCA TCGGGATTAC TGCTTAAGAC TTATGGTGGT CATGCGGATG AAGTCACCGA 
0061 TGCGGCGGGA AGCTGTGATA GTAGCTATAT CGTTTCCGCC AGCTTGGATA AAAGCATTAT 
0121 CTACTGGGAC GTGTCAACGG GAGCACCAGT ACGGCGCCTG CGTAGCCATG CCGGAGGTGT 
0181 TAGATGCGTC TGCTTCAACG AGGACTCCTC GATAGCCATT TCCGGGGGTC GAGATAATGC 
0241 CGTAATGTGC TGGGACATTC GAACGCGTCG CCTGGATCCC GTTCAGGTGA TGAAGGAGGC 
0301 CCGGGACTGC ATCACCACGG TGGCAACCAA CGAGAATCGC ATCTACGCCG CCTCCTTGGA 
0361 CGGATGTGTG AGGACCTACG ATATTCGGGT GGGCGAACTT ACCTGCGACA AAATTGGTGA 
0421 ACCCATCACA TAT-CTGGCG CAGACGCGCG ACGAGCAATG TCTGGTGGCC GGCTGTCAGG 
0481 ATAGTGTGGT TCGTCTGCTG G

Assemble Data


4935R-1.IR_full       1   GAATCCGGCATCGGGATTACTGCTTAAGACTTATGGTGGTCATGCGGATGAAGTCACCGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     1   GAATCCGGCATCGGGATTACTGCTTAAGACTTATGGTGGTCATGCGGATGAAGTCACCGA 60


4935R-1.IR_full       61  TGCGGCGGGAAGCTGTGATAGTAGCTATATCGTTTCCGCCAGCTTGGATAAAAGCATTAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     61  TGCGGCGGGAAGCTGTGATAGTAGCTATATCGTTTCCGCCAGCTTGGATAAAAGCATTAT 120


4935R-1.IR_full       121 CTACTGGGACGTGTCAACGGGAGCACCAGTACGGCGCCTGCGTAGCCATGCCGGAGGTGT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     121 CTACTGGGACGTGTCAACGGGAGCACCAGTACGGCGCCTGCGTAGCCATGCCGGAGGTGT 180


4935R-1.IR_full       181 TAGATGCGTCTGCTTCAACGAGGACTCCTCGATAGCCATTTCCGGGGGTCGAGATAATGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     181 TAGATGCGTCTGCTTCAACGAGGACTCCTCGATAGCCATTTCCGGGGGTCGAGATAATGC 240


4935R-1.IR_full       241 CGTAATGTGCTGGGACATTCGAACGCGTCGCCTGGATCCCGTTCAGGTGATGAAGGAGGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     241 CGTAATGTGCTGGGACATTCGAACGCGTCGCCTGGATCCCGTTCAGGTGATGAAGGAGGC 300


4935R-1.IR_full       301 CCGGGACTGCATCACCACGGTGGCAACCAACGAGAATCGCATCTACGCCGCCTCCTTGGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     301 CCGGGACTGCATCACCACGGTGGCAACCAACGAGAATCGCATCTACGCCGCCTCCTTGGA 360


4935R-1.IR_full       361 CGGATGTGTGAGGACCTACGATATTCGGGTGGGCGAACTTACCTGCGACAAAATTGGTGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     361 CGGATGTGTGAGGACCTACGATATTCGGGTGGGCGAACTTACCTGCGACAAAATTGGTGA 420


4935R-1.IR_full       421 ACCCATCACATATNCTGGCGCAGACGCGCGACGAGCAATGTCTGGTGGCCGGCTGTCAGG 480
                          ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
4935R-1.in silico     421 ACCCATCACATAT-CTGGCGCAGACGCGCGACGAGCAATGTCTGGTGGCCGGCTGTCAGG 480


4935R-1.IR_full       481 ATAGTGTGGTTCGTCTGCTGG 501
                          |||||||||||||||||||||
4935R-1.in silico     481 ATAGTGTGGTTCGTCTGCTGG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135938.2	CG4935-RA (CG4935), mRNA
0	0	3	2	2	NM_130652.2	CG8310-RA (CG8310), mRNA
0	0	2	3	2	NM_132716.1	CG13403-RA (CG13403), mRNA
0	0	1	4	3	NM_169672.1	CG4699-RB, transcript variant B (CG4699), mRNA
0	0	1	4	3	NM_142235.1	CG4699-RA, transcript variant A (CG4699), mRNA
0	0	1	4	3	NM_169673.1	CG4699-RC, transcript variant C (CG4699), mRNA
0	0	1	4	2	NM_137032.2	CG5970-RA (CG5970), mRNA
0	0	1	3	2	NM_130639.2	CG2918-RA (CG2918), mRNA
0	0	1	2	2	NM_132431.2	CG1655-RA (sofe), mRNA
0	0	0	5	8	NM_132779.1	CG15028-RB, transcript variant B (CG15028), mRNA
0	0	0	5	8	NM_167431.1	CG15028-RA, transcript variant A (CG15028), mRNA
0	0	0	5	4	NM_139680.1	CG17150-RA, transcript variant A (CG17150), mRNA
0	0	0	5	3	NM_176581.2	CG33204-RA (CG33204), mRNA
0	0	0	3	9	NM_078981.2	CG8967-RA (otk), mRNA
0	0	0	3	8	NM_168153.1	CG32406-RA (CG32406), mRNA
0	0	0	3	7	NM_167170.1	CG10701-RC, transcript variant C (Moe), mRNA
0	0	0	3	7	NM_167169.1	CG10701-RA, transcript variant A (Moe), mRNA
0	0	0	3	7	NM_167168.1	CG10701-RB, transcript variant B (Moe), mRNA
0	0	0	3	7	NM_080343.2	CG10701-RD, transcript variant D (Moe), mRNA
0	0	0	3	7	NM_206664.1	CG10701-RJ, transcript variant J (Moe), mRNA
0	0	0	3	7	NM_206665.1	CG10701-RI, transcript variant I (Moe), mRNA
0	0	0	3	7	NM_206669.1	CG10701-RE, transcript variant E (Moe), mRNA
0	0	0	3	7	NM_206666.1	CG10701-RH, transcript variant H (Moe), mRNA
0	0	0	3	7	NM_206668.1	CG10701-RF, transcript variant F (Moe), mRNA
0	0	0	3	7	NM_206667.1	CG10701-RG, transcript variant G (Moe), mRNA
0	0	0	3	6	NM_132981.1	CG8675-RA (CG8675), mRNA
0	0	0	3	6	NM_135657.1	CG14925-RA (Osi21), mRNA
0	0	0	3	5	NM_143647.1	CG2053-RA (CG2053), mRNA
0	0	0	3	3	NM_132703.2	CG32626-RC, transcript variant C (CG32626), mRNA
0	0	0	3	3	NM_134961.1	CG10019-RA (CG10019), mRNA

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