NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4933R-1

Symbol

CG4933

Full Name

CG4933

CG No

CG4933

Old CG No

CG4933

Synonyms

CG4933

Accession No (Link to NCBI)

NM_140623.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGCTCCTCCTTTGCTGGTTG

Primer Seq. 3'

aatctagaggtaccGATGGTCTCCTGCAGCGAATA

Predicted Fragment Size

IR fragment full Seq

0001 TGGCTCCTCC TTTGCTGGTT GGCGCCATTG TGGCTCGCAC TTTGTCGCTC TTGTGGAATA 
0061 TCCCGCTGCT GGGCGTAAAT CACTGCATTG GACACATCGA GATGGGTCGC CTGATCACCG 
0121 GTGCCCAGAA TCCTACAGTT CTCTATGTGA GCGGTGGAAA CACACAGGTT ATTGCCTACT 
0181 CCAATAAGCG ATATCGCATC TTTGGCGAAA CTATAGACAT TGCAGTGGGC AATTGCCTGG 
0241 ATCGATTCGC CCGCATCATT AAGCTGTCCA ACGATCCTAG TCCGGGCTAC AACATCGAGC 
0301 AGCTGGCCAA GTCGAGCAAT CGTTATATTA AACTGCCCTA TGTGGTTAAG GGCATGGATG 
0361 TCAGCTTCTC GGGGATTTTA TCCTACATCG AGGACCTGGC AGAGCCGGGC AAGAGGCAAA 
0421 ATAAGAGAAA AAAGCCACAG GAAGAAGAGG TCAATAATTA CAGCCAAGCC GATCTTTGCT 
0481 ATTCGCTGCA GGAGACCATC

in silico PCR Fragment

0001 TGGCTCCTCC TTTGCTGGTT GGCGCCATTG TGGCTCGCAC TTTGTCGCTC TTGTGGAATA 
0061 TCCCGCTGCT GGGCGTAAAT CACTGCATTG GACACATCGA GATGGGTCGC CTGATCACCG 
0121 GTGCCCAGAA TCCTACAGTT CTCTATGTGA GCGGTGGAAA CACACAGGTT ATTGCCTACT 
0181 CCAATAAGCG ATATCGCATC TTTGGCGAAA CTATAGACAT TGCAGTGGGC AATTGCCTGG 
0241 ATCGATTCGC CCGCATCATT AAGCTGTCCA ACGATCCTAG TCCGGGCTAC AACATCGAGC 
0301 AGCTGGCCAA GTCGAGCAAT CGTTATATTA AACTGCCCTA TGTGGTTAAG GGCATGGATG 
0361 TCAGCTTCTC GGGGATTTTA TCCTACATCG AGGACCTGGC AGAGCCGGGC AAGAGGCAAA 
0421 ATAAGAGAAA AAAGCCACAG GAAGAAGAGG TCAATAATTA CAGCCAAGCC GATCTTTGCT 
0481 ATTCGCTGCA GGAGACCATC

Assemble Data

<PRE>

4933R-1.IR_full       1   TGGCTCCTCCTTTGCTGGTTGGCGCCATTGTGGCTCGCACTTTGTCGCTCTTGTGGAATA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     1   TGGCTCCTCCTTTGCTGGTTGGCGCCATTGTGGCTCGCACTTTGTCGCTCTTGTGGAATA 60


4933R-1.IR_full       61  TCCCGCTGCTGGGCGTAAATCACTGCATTGGACACATCGAGATGGGTCGCCTGATCACCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     61  TCCCGCTGCTGGGCGTAAATCACTGCATTGGACACATCGAGATGGGTCGCCTGATCACCG 120


4933R-1.IR_full       121 GTGCCCAGAATCCTACAGTTCTCTATGTGAGCGGTGGAAACACACAGGTTATTGCCTACT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     121 GTGCCCAGAATCCTACAGTTCTCTATGTGAGCGGTGGAAACACACAGGTTATTGCCTACT 180


4933R-1.IR_full       181 CCAATAAGCGATATCGCATCTTTGGCGAAACTATAGACATTGCAGTGGGCAATTGCCTGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     181 CCAATAAGCGATATCGCATCTTTGGCGAAACTATAGACATTGCAGTGGGCAATTGCCTGG 240


4933R-1.IR_full       241 ATCGATTCGCCCGCATCATTAAGCTGTCCAACGATCCTAGTCCGGGCTACAACATCGAGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     241 ATCGATTCGCCCGCATCATTAAGCTGTCCAACGATCCTAGTCCGGGCTACAACATCGAGC 300


4933R-1.IR_full       301 AGCTGGCCAAGTCGAGCAATCGTTATATTAAACTGCCCTATGTGGTTAAGGGCATGGATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     301 AGCTGGCCAAGTCGAGCAATCGTTATATTAAACTGCCCTATGTGGTTAAGGGCATGGATG 360


4933R-1.IR_full       361 TCAGCTTCTCGGGGATTTTATCCTACATCGAGGACCTGGCAGAGCCGGGCAAGAGGCAAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     361 TCAGCTTCTCGGGGATTTTATCCTACATCGAGGACCTGGCAGAGCCGGGCAAGAGGCAAA 420


4933R-1.IR_full       421 ATAAGAGAAAAAAGCCACAGGAAGAAGAGGTCAATAATTACAGCCAAGCCGATCTTTGCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4933R-1.in silico     421 ATAAGAGAAAAAAGCCACAGGAAGAAGAGGTCAATAATTACAGCCAAGCCGATCTTTGCT 480


4933R-1.IR_full       481 ATTCGCTGCAGGAGACCATC 500
                          ||||||||||||||||||||
4933R-1.in silico     481 ATTCGCTGCAGGAGACCATC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140623.2	CG4933-RA (CG4933), mRNA
0.41	2	2	2	3	NM_141852.1	CG12594-RA (CG12594), mRNA
0	0	2	5	3	NM_078771.2	CG11326-RA, transcript variant A (Tsp), mRNA
0	0	2	3	5	NM_165814.2	CG9062-RB (CG9062), mRNA
0	0	2	3	3	NM_137400.2	CG6446-RA, transcript variant A (Sema-1b), mRNA
0	0	2	3	3	NM_206157.1	CG6446-RB, transcript variant B (Sema-1b), mRNA
0	0	2	2	4	NM_139644.1	CG15020-RA (CG15020), mRNA
0	0	2	2	4	NM_139425.1	CG5691-RA (CG5691), mRNA
0	0	1	6	4	NM_078859.2	CG5813-RA, transcript variant A (chif), mRNA
0	0	1	6	4	NM_165156.1	CG5813-RB, transcript variant B (chif), mRNA
0	0	1	4	7	NM_079985.2	CG5206-RA (bon), mRNA
0	0	1	3	3	NM_142049.2	CG9611-RB, transcript variant B (CG9611), mRNA
0	0	1	3	3	NM_169550.1	CG9611-RA, transcript variant A (CG9611), mRNA
0	0	1	3	3	NM_142081.2	CG3172-RA (twf), mRNA
0	0	1	2	6	NM_079453.2	CG6975-RA (gig), mRNA
0	0	1	2	5	NM_140776.2	CG13699-RA (CG13699), mRNA
0	0	1	2	3	NM_130542.2	CG11403-RA (CG11403), mRNA
0	0	1	2	3	NM_141497.1	CG2781-RA (CG2781), mRNA
0	0	1	2	2	NR_002020.1	CR32865, miscRNA
0	0	0	6	2	NM_165602.1	CG30365-RA (CG30365), mRNA
0	0	0	5	6	NM_057576.3	CG6407-RA (Wnt5), mRNA
0	0	0	5	5	NM_079138.1	CG2692-RA (gsb-n), mRNA
0	0	0	5	4	NM_057784.4	CG7833-RA (Orc5), mRNA
0	0	0	4	7	NM_140872.1	CG9330-RA (CG9330), mRNA
0	0	0	4	6	NM_001042909.1	CG31619-RC, transcript variant C (CG31619), mRNA
0	0	0	4	6	NM_165396.1	CG31619-RA, transcript variant A (CG31619), mRNA
0	0	0	4	6	NM_165397.1	CG31619-RB, transcript variant B (CG31619), mRNA
0	0	0	4	3	NM_139604.2	CG14998-RC, transcript variant C (CG14998), mRNA
0	0	0	4	3	NM_168061.2	CG14998-RB, transcript variant B (CG14998), mRNA
0	0	0	4	3	NM_142490.2	CG7714-RA (CG7714), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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