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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4930R-4

Symbol CG4930 Full Name CG4930

CG No CG4930 Old CG No CG4930

Synonyms BG:DS07473.2, CG4930

Accession No (Link to NCBI) NM_135937.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCAATCGAACACAGAGAAGG

Primer Seq. 3'

aatctagaggtaccTCTTCTTCACGCTGGCCTGGT

Predicted Fragment Size

IR fragment full Seq

0001 CGCAATCGAA CACAGAGAAG GCCAGCCAAT TGCTGGAGGA ATACCGTGGT GACGCCTGCT 
0061 TCTACGACCC GACGCCGTAC AACGAATGGA TTGTCAAGCT GAGGGATGAG GTTCTAAAGA 
0121 AGGAACTCCT CGACTTTTGG CGCGATGTGC TGGTGAAGAA GCAACTCGGT CCCTGTTGGT 
0181 CACGCGACAG TGATCTCTTT GACAGCGACG ACACCCCGCC ATTGGAGTTC TATGCCCATG 
0241 CCGGTTGTAC CGCTCCATTT GCAGCCAGCT TAAAGGTGCG CGCAGCACTC GAGGAGCAGG 
0301 CTTCGCTGGA TCAGGATGGG CCAGCGACGC CTACAACTCC GGGTGAACTG AGTGCCGATG 
0361 ATGCCGCCGC TCTGTCTGGC GAATTTGAGG CCACCCTGAC CAAGGAAAAT CCTCTCGAAG 
0421 AATACCGAAC GCTAATGAAG CGCTTTGTCC TGACCAAAAT CATCGTTCCG GACAGCGTTC 
0481 ACCAGGCCAG CGTGAAGAAG A

in silico PCR Fragment

0001 CGCAATCGAA CACAGAGAAG GCCAGCCAAT TGCTGGAGGA ATACCGTGGT GACGCCTGCT 
0061 TCTACGACCC GACGCCGTAC AACGAATGGA TTGTCAAGCT GAGGGATGAG GTTCTAAAGA 
0121 AGGAACTCCT CGACTTTTGG CGCGATGTGC TGGTGAAGAA GCAACTCGGT CCCTGTTGGT 
0181 CACGCGACAG TGATCTCTTT GACAGCGACG ACACCCCGCC ATTGGAGTTC TATGCCCATG 
0241 CCGGTTGTAC CGCTCCATTT GCAGCCAGCT TAAAGGTGCG CGCAGCACTC GAGGAGCAGG 
0301 CTTCGCTGGA TCAGGATGGG CCAGCGACGC CTACAACTCC GGGTGAACTG AGTGCCGATG 
0361 ATGCCGCCGC TCTGTCTGGC GAATTTGAGG CCACCCTGAC CAAGGAAAAT CCGCTCGAGG 
0421 AATACCGAAC TCTAATGAAG CGCTTTGTTC TGACCAAAAT CATCGTTCCG GACAGCGTTC 
0481 ACCAGGCCAG CGTGAAGAAG A

Assemble Data


4930R-4.IR_full       1   CGCAATCGAACACAGAGAAGGCCAGCCAATTGCTGGAGGAATACCGTGGTGACGCCTGCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4930R-4.in silico     1   CGCAATCGAACACAGAGAAGGCCAGCCAATTGCTGGAGGAATACCGTGGTGACGCCTGCT 60


4930R-4.IR_full       61  TCTACGACCCGACGCCGTACAACGAATGGATTGTCAAGCTGAGGGATGAGGTTCTAAAGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4930R-4.in silico     61  TCTACGACCCGACGCCGTACAACGAATGGATTGTCAAGCTGAGGGATGAGGTTCTAAAGA 120


4930R-4.IR_full       121 AGGAACTCCTCGACTTTTGGCGCGATGTGCTGGTGAAGAAGCAACTCGGTCCCTGTTGGT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4930R-4.in silico     121 AGGAACTCCTCGACTTTTGGCGCGATGTGCTGGTGAAGAAGCAACTCGGTCCCTGTTGGT 180


4930R-4.IR_full       181 CACGCGACAGTGATCTCTTTGACAGCGACGACACCCCGCCATTGGAGTTCTATGCCCATG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4930R-4.in silico     181 CACGCGACAGTGATCTCTTTGACAGCGACGACACCCCGCCATTGGAGTTCTATGCCCATG 240


4930R-4.IR_full       241 CCGGTTGTACCGCTCCATTTGCAGCCAGCTTAAAGGTGCGCGCAGCACTCGAGGAGCAGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4930R-4.in silico     241 CCGGTTGTACCGCTCCATTTGCAGCCAGCTTAAAGGTGCGCGCAGCACTCGAGGAGCAGG 300


4930R-4.IR_full       301 CTTCGCTGGATCAGGATGGGCCAGCGACGCCTACAACTCCGGGTGAACTGAGTGCCGATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4930R-4.in silico     301 CTTCGCTGGATCAGGATGGGCCAGCGACGCCTACAACTCCGGGTGAACTGAGTGCCGATG 360


4930R-4.IR_full       361 ATGCCGCCGCTCTGTCTGGCGAATTTGAGGCCACCCTGACCAAGGAAAATCCTCTCGAAG 420
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| |
4930R-4.in silico     361 ATGCCGCCGCTCTGTCTGGCGAATTTGAGGCCACCCTGACCAAGGAAAATCCGCTCGAGG 420


4930R-4.IR_full       421 AATACCGAACGCTAATGAAGCGCTTTGTCCTGACCAAAATCATCGTTCCGGACAGCGTTC 480
                          |||||||||| ||||||||||||||||| |||||||||||||||||||||||||||||||
4930R-4.in silico     421 AATACCGAACTCTAATGAAGCGCTTTGTTCTGACCAAAATCATCGTTCCGGACAGCGTTC 480


4930R-4.IR_full       481 ACCAGGCCAGCGTGAAGAAGA 501
                          |||||||||||||||||||||
4930R-4.in silico     481 ACCAGGCCAGCGTGAAGAAGA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	483	7	14	9	NM_135937.2	CG4930-RA (CG4930), mRNA
0	0	2	2	3	NM_141644.2	CG9379-RA (by), mRNA
0	0	2	2	2	NM_137587.1	CG15122-RA (CG15122), mRNA
0	0	1	4	2	NM_136878.2	CG8364-RB, transcript variant B (Rep3), mRNA
0	0	1	3	4	NM_057517.2	CG4824-RA, transcript variant A (BicC), mRNA
0	0	1	3	4	NM_165144.1	CG4824-RB, transcript variant B (BicC), mRNA
0	0	1	3	4	NM_165145.1	CG4824-RD, transcript variant D (BicC), mRNA
0	0	1	2	4	NM_141044.2	CG11309-RA, transcript variant A (CG11309), mRNA
0	0	1	2	4	NM_133024.2	CG6769-RA (CG6769), mRNA
0	0	1	2	4	NM_168896.1	CG11309-RB, transcript variant B (CG11309), mRNA
0	0	1	2	3	NM_140583.1	CG12272-RA (CG12272), mRNA
0	0	1	2	2	NM_140310.1	CG4328-RA (CG4328), mRNA
0	0	1	2	1	NM_132343.2	CG3002-RB (Gga), mRNA
0	0	0	6	9	NM_057391.3	CG1977-RA (alpha-Spec), mRNA
0	0	0	4	6	NM_170480.1	CG2216-RC, transcript variant C (Fer1HCH), mRNA
0	0	0	4	6	NM_170481.1	CG2216-RD, transcript variant D (Fer1HCH), mRNA
0	0	0	4	6	NM_170482.1	CG2216-RE, transcript variant E (Fer1HCH), mRNA
0	0	0	4	6	NM_170479.1	CG2216-RB, transcript variant B (Fer1HCH), mRNA
0	0	0	4	6	NM_080134.2	CG2216-RA, transcript variant A (Fer1HCH), mRNA
0	0	0	4	6	NM_131990.1	CG12732-RA (CG12732), mRNA
0	0	0	4	4	NM_137891.1	CG3695-RA (MED23), mRNA
0	0	0	4	4	NM_080516.1	CG16757-RA (Spn), mRNA
0	0	0	4	4	NM_170620.2	CG12052-RD, transcript variant D (lola), mRNA
0	0	0	4	4	NM_170621.3	CG12052-RE, transcript variant E (lola), mRNA
0	0	0	4	3	NM_141173.1	CG11133-RA (CG11133), mRNA
0	0	0	4	3	NM_142815.2	CG6949-RA (mRpL45), mRNA
0	0	0	4	2	NM_143233.2	CG31080-RA (CG31080), mRNA
0	0	0	3	8	NM_134789.1	CG15356-RA (CG15356), mRNA
0	0	0	3	6	NM_169806.1	CG31235-RA (CG31235), mRNA
0	0	0	3	5	NM_141453.2	CG1234-RA (CG1234), mRNA

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