National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4893R-3 
 Symbol CG4893  Full Name CG4893 
 CG No CG4893  Old CG No CG4893 
 Synonyms CG4893 
 Accession No (Link to NCBI) NM_140624.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAGCAAATGGAAGCCCCTCCC 
 Primer Seq. 3' 
aatctagaggtaccACGTTCCGTCTGCCCGCGATA 
 Predicted Fragment Size 
499 
 IR fragment full Seq 
0001 TAGCAAATGG AAGCCCCTCC CCAGCTCCAA CTCCTGAGGT GCCCGCTACC GAGTTGGCCC 
0061 AATTGGCGCT GGAGGATGAG CCCAAGGTTA GCTTTTCGGA TCAGTTCAAG GCCTTCTCCA 
0121 AGTTCGGGGA CTCCAAGTCC GATGGCAAGC TGATAACGCT GTCGCAGAGT GACAAGTGGA 
0181 TGAAACAGGC CAAAGTTATC GACAAGAAGA TCACCACCAC GGATACAGGC ATCCACTTCA 
0241 AGAAGTTCAA GGCCATGAAG ATCTCGCTCA GCGACTACAA CAAATTCCTG GACGATCTGG 
0301 CCAAGACGAA AAAGGTGGAG CTGTCGGAGA TCAAGCAAAA GTTGGCCAGT TGTGGAGCTC 
0361 CAGGAGTTGT GTCCGTTTCG GCTGGAAAAG CCGCAGCTGC CGTGGATCGA TTGACAGATA 
0421 CGAGCAAGTA CACCGGTTCC CACAAGGAGC GTTTCGATGC CTCCGGAAAG GGCAAGGGTA 
0481 TCGCGGGCAG ACGGAACTGT  
 in silico PCR Fragment 
0001 CAGCAAATGG AAGCCCCTCC CCAGCGCCAA CTCCTGAGGT GCCCGCTACC GAGTTGGCCC 
0061 AATTGGCGCT GGAGGATGAG CCCAAGGTTA GCTTTTCGGA TCAGTTCAAG GCCTTCTCCA 
0121 AGTTCGGGGA CTCCAAGTCC GATGGCAAGC TGATAACGCT GTCGCAGAGT GACAAGTGGA 
0181 TGAAACAGGC CAAAGTTATC GACAAGAAGA TCACCACCAC GGATACAGGC ATCCACTTCA 
0241 AGAAGTTCAA GGCCATGAAG ATCTCGCTCA GCGACTACAA CAAATTCCTG GACGATCTGG 
0301 CCAAGACGAA AAAGGTGGAG CTGTCGGAGA TCAAGCAAAA GTTGGCCAGT TGTGGAGCTC 
0361 CAGGAGTTGT GTCCGTTTCG GCTGGAAAAG CCGCAGCTGC CGTGGATCGA TTGACGGATA 
0421 CGAGCAAGTA CACCGGTTCC CACAAGGAGC GTTTCGATGC CTCCGGAAAG GGCAAGGGTA 
0481 TCGCGGGCAG ACGGAAC-GT  
 Assemble Data 
<PRE>

4893R-3.IR_full       1   <span class="snp"><tt>T</tt></span>AGCAAATGGAAGCCCCTCCCCAGC<span class="snp"><tt>T</tt></span>CCAACTCCTGAGGTGCCCGCTACCGAGTTGGCCC 60
                          <span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
4893R-3.in silico     1   <span class="snp"><tt>C</tt></span>AGCAAATGGAAGCCCCTCCCCAGC<span class="snp"><tt>G</tt></span>CCAACTCCTGAGGTGCCCGCTACCGAGTTGGCCC 60


4893R-3.IR_full       61  AATTGGCGCTGGAGGATGAGCCCAAGGTTAGCTTTTCGGATCAGTTCAAGGCCTTCTCCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-3.in silico     61  AATTGGCGCTGGAGGATGAGCCCAAGGTTAGCTTTTCGGATCAGTTCAAGGCCTTCTCCA 120


4893R-3.IR_full       121 AGTTCGGGGACTCCAAGTCCGATGGCAAGCTGATAACGCTGTCGCAGAGTGACAAGTGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-3.in silico     121 AGTTCGGGGACTCCAAGTCCGATGGCAAGCTGATAACGCTGTCGCAGAGTGACAAGTGGA 180


4893R-3.IR_full       181 TGAAACAGGCCAAAGTTATCGACAAGAAGATCACCACCACGGATACAGGCATCCACTTCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-3.in silico     181 TGAAACAGGCCAAAGTTATCGACAAGAAGATCACCACCACGGATACAGGCATCCACTTCA 240


4893R-3.IR_full       241 AGAAGTTCAAGGCCATGAAGATCTCGCTCAGCGACTACAACAAATTCCTGGACGATCTGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-3.in silico     241 AGAAGTTCAAGGCCATGAAGATCTCGCTCAGCGACTACAACAAATTCCTGGACGATCTGG 300


4893R-3.IR_full       301 CCAAGACGAAAAAGGTGGAGCTGTCGGAGATCAAGCAAAAGTTGGCCAGTTGTGGAGCTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-3.in silico     301 CCAAGACGAAAAAGGTGGAGCTGTCGGAGATCAAGCAAAAGTTGGCCAGTTGTGGAGCTC 360


4893R-3.IR_full       361 CAGGAGTTGTGTCCGTTTCGGCTGGAAAAGCCGCAGCTGCCGTGGATCGATTGAC<span class="snp"><tt>A</tt></span>GATA 420
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
4893R-3.in silico     361 CAGGAGTTGTGTCCGTTTCGGCTGGAAAAGCCGCAGCTGCCGTGGATCGATTGAC<span class="snp"><tt>G</tt></span>GATA 420


4893R-3.IR_full       421 CGAGCAAGTACACCGGTTCCCACAAGGAGCGTTTCGATGCCTCCGGAAAGGGCAAGGGTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-3.in silico     421 CGAGCAAGTACACCGGTTCCCACAAGGAGCGTTTCGATGCCTCCGGAAAGGGCAAGGGTA 480


4893R-3.IR_full       481 TCGCGGGCAGACGGAAC<span class="snp"><tt>T</tt></span>GT 500
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
4893R-3.in silico     481 TCGCGGGCAGACGGAAC<span class="snp"><tt>-</tt></span>GT 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   481  0  0  0  NM_140624.2  CG4893-RA (CG4893), mRNA 
0   0  3  4  2  NM_136482.1  CG1882-RA, transcript variant A (CG1882), mRNA 
0   0  3  4  2  NM_165562.1  CG1882-RD, transcript variant D (CG1882), mRNA 
0   0  3  4  2  NM_165561.1  CG1882-RC, transcript variant C (CG1882), mRNA 
0   0  3  4  2  NM_165560.1  CG1882-RB, transcript variant B (CG1882), mRNA 
0   0  2  4  2  NM_144347.2  CG12149-RA (c12.2), mRNA 
0   0  2  3  5  NM_143409.1  CG11873-RA (CG11873), mRNA 
0   0  2  3  3  NM_165761.1  CG18408-RD, transcript variant D (CAP), mRNA 
0   0  2  3  3  NM_130651.2  CG13758-RA (CG13758), mRNA 
0   0  2  3  2  NM_078545.2  CG12653-RA (btd), mRNA 
0   0  2  2  4  NM_132065.1  CG4660-RA (CG4660), mRNA 
0   0  2  2  4  NM_135795.1  CG9395-RA (CG9395), mRNA 
0   0  2  2  2  NM_080147.2  CG5965-RA (woc), mRNA 
0   0  1  6  7  NM_134511.1  CG12702-RA (CG12702), mRNA 
0   0  1  6  2  NM_144452.1  CG13713-RA (CG13713), mRNA 
0   0  1  4  4  NM_142828.1  CG13842-RA (CG13842), mRNA 
0   0  1  3  4  NM_137318.2  CG5859-RA (CG5859), mRNA 
0   0  1  3  3  NM_167294.1  CG2371-RC, transcript variant C (CG2371), mRNA 
0   0  1  3  3  NM_167293.1  CG2371-RA, transcript variant A (CG2371), mRNA 
0   0  1  3  3  NM_132510.2  CG2371-RB, transcript variant B (CG2371), mRNA 
0   0  1  3  3  NM_137486.3  CG17533-RA (GstE8), mRNA 
0   0  1  3  3  NM_164902.1  CG31717-RA (CG31717), mRNA 
0   0  1  2  6  NM_057941.3  CG5227-RA, transcript variant A (sdk), mRNA 
0   0  1  2  6  NM_134314.2  CG5227-RB, transcript variant B (sdk), mRNA 
0   0  1  2  6  NM_134315.2  CG5227-RD, transcript variant D (sdk), mRNA 
0   0  1  2  6  NM_057942.3  CG5227-RC, transcript variant C (sdk), mRNA 
0   0  1  2  5  NM_130498.1  CG13362-RA (CG13362), mRNA 
0   0  1  2  4  NM_140325.1  CG10682-RA (vih), mRNA 
0   0  1  2  2  NM_164741.1  CG4502-RB, transcript variant B (CG4502), mRNA 
0   0  1  2  2  NM_135259.2  CG4502-RA, transcript variant A (CG4502), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.