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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4893R-2 |
| Symbol |
CG4893 |
Full Name |
CG4893 |
| CG No |
CG4893 |
Old CG No |
CG4893 |
| Synonyms |
CG4893 |
| Accession No (Link to NCBI) |
NM_140624.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAGCAAATGGAAGCCCCTCCC |
| Primer Seq. 3' |
aatctagaggtaccACGTTCCGTCTGCCCGCGATA |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 TAGCAAATGG AAGCCCCTCC CCAGCTCCAA CTCCTGAGGT GCCCGCTACC GAGTTGGCCC
0061 AATTGGCGCT GGAGGATGAG CCCAAGGTTA GCTTTTCGGA TCAGTTCAAG GCCTTCTCCA
0121 AGTTCGGGGA CTCCAAGTCC GATGGCAAGC TGATAACGCT GTCGCAGAGT GACAAGTGGA
0181 TGAAACAGGC CAAAGTTATC GACAAGAAGA TCACCACCAC GGATACAGGC ATCCACTTCA
0241 AGAAGTTCAA GGCCATGAAG ATCTCGCTCA GCGACTACAA CAAATTCCTG GACGATCTGG
0301 CCAAGACGAA AAAGGTGGAG CTGTCGGAGA TCAAGCAAAA GTTGGCCAGT TGTGGAGCTC
0361 CAGGAGTTGT GTCCGTTTCG GCTGGAAAAG CCGCAGCTGC CGTGGATCGA TTGACAGATA
0421 CGAGCAAGTA CACCGGTTCC CACAAGGAGC GTTTCGATGC CTCCGGAAAG GGCAAGGGTA
0481 TCGCGGGCAG ACGGAACTGT |
| in silico PCR Fragment |
0001 CAGCAAATGG AAGCCCCTCC CCAGCGCCAA CTCCTGAGGT GCCCGCTACC GAGTTGGCCC
0061 AATTGGCGCT GGAGGATGAG CCCAAGGTTA GCTTTTCGGA TCAGTTCAAG GCCTTCTCCA
0121 AGTTCGGGGA CTCCAAGTCC GATGGCAAGC TGATAACGCT GTCGCAGAGT GACAAGTGGA
0181 TGAAACAGGC CAAAGTTATC GACAAGAAGA TCACCACCAC GGATACAGGC ATCCACTTCA
0241 AGAAGTTCAA GGCCATGAAG ATCTCGCTCA GCGACTACAA CAAATTCCTG GACGATCTGG
0301 CCAAGACGAA AAAGGTGGAG CTGTCGGAGA TCAAGCAAAA GTTGGCCAGT TGTGGAGCTC
0361 CAGGAGTTGT GTCCGTTTCG GCTGGAAAAG CCGCAGCTGC CGTGGATCGA TTGACGGATA
0421 CGAGCAAGTA CACCGGTTCC CACAAGGAGC GTTTCGATGC CTCCGGAAAG GGCAAGGGTA
0481 TCGCGGGCAG ACGGAAC-GT |
| Assemble Data |
<PRE>
4893R-2.IR_full 1 <span class="snp"><tt>T</tt></span>AGCAAATGGAAGCCCCTCCCCAGC<span class="snp"><tt>T</tt></span>CCAACTCCTGAGGTGCCCGCTACCGAGTTGGCCC 60
<span class="snp"><tt> </tt></span>||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||
4893R-2.in silico 1 <span class="snp"><tt>C</tt></span>AGCAAATGGAAGCCCCTCCCCAGC<span class="snp"><tt>G</tt></span>CCAACTCCTGAGGTGCCCGCTACCGAGTTGGCCC 60
4893R-2.IR_full 61 AATTGGCGCTGGAGGATGAGCCCAAGGTTAGCTTTTCGGATCAGTTCAAGGCCTTCTCCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-2.in silico 61 AATTGGCGCTGGAGGATGAGCCCAAGGTTAGCTTTTCGGATCAGTTCAAGGCCTTCTCCA 120
4893R-2.IR_full 121 AGTTCGGGGACTCCAAGTCCGATGGCAAGCTGATAACGCTGTCGCAGAGTGACAAGTGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-2.in silico 121 AGTTCGGGGACTCCAAGTCCGATGGCAAGCTGATAACGCTGTCGCAGAGTGACAAGTGGA 180
4893R-2.IR_full 181 TGAAACAGGCCAAAGTTATCGACAAGAAGATCACCACCACGGATACAGGCATCCACTTCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-2.in silico 181 TGAAACAGGCCAAAGTTATCGACAAGAAGATCACCACCACGGATACAGGCATCCACTTCA 240
4893R-2.IR_full 241 AGAAGTTCAAGGCCATGAAGATCTCGCTCAGCGACTACAACAAATTCCTGGACGATCTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-2.in silico 241 AGAAGTTCAAGGCCATGAAGATCTCGCTCAGCGACTACAACAAATTCCTGGACGATCTGG 300
4893R-2.IR_full 301 CCAAGACGAAAAAGGTGGAGCTGTCGGAGATCAAGCAAAAGTTGGCCAGTTGTGGAGCTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-2.in silico 301 CCAAGACGAAAAAGGTGGAGCTGTCGGAGATCAAGCAAAAGTTGGCCAGTTGTGGAGCTC 360
4893R-2.IR_full 361 CAGGAGTTGTGTCCGTTTCGGCTGGAAAAGCCGCAGCTGCCGTGGATCGATTGAC<span class="snp"><tt>A</tt></span>GATA 420
|||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||
4893R-2.in silico 361 CAGGAGTTGTGTCCGTTTCGGCTGGAAAAGCCGCAGCTGCCGTGGATCGATTGAC<span class="snp"><tt>G</tt></span>GATA 420
4893R-2.IR_full 421 CGAGCAAGTACACCGGTTCCCACAAGGAGCGTTTCGATGCCTCCGGAAAGGGCAAGGGTA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4893R-2.in silico 421 CGAGCAAGTACACCGGTTCCCACAAGGAGCGTTTCGATGCCTCCGGAAAGGGCAAGGGTA 480
4893R-2.IR_full 481 TCGCGGGCAGACGGAAC<span class="snp"><tt>T</tt></span>GT 500
|||||||||||||||||<span class="snp"><tt> </tt></span>||
4893R-2.in silico 481 TCGCGGGCAGACGGAAC<span class="snp"><tt>-</tt></span>GT 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
0 |
NM_140624.2 |
CG4893-RA (CG4893), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_136482.1 |
CG1882-RA, transcript variant A (CG1882), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_165562.1 |
CG1882-RD, transcript variant D (CG1882), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_165561.1 |
CG1882-RC, transcript variant C (CG1882), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_165560.1 |
CG1882-RB, transcript variant B (CG1882), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_144347.2 |
CG12149-RA (c12.2), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_143409.1 |
CG11873-RA (CG11873), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_165761.1 |
CG18408-RD, transcript variant D (CAP), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_130651.2 |
CG13758-RA (CG13758), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_078545.2 |
CG12653-RA (btd), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132065.1 |
CG4660-RA (CG4660), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135795.1 |
CG9395-RA (CG9395), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_080147.2 |
CG5965-RA (woc), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_134511.1 |
CG12702-RA (CG12702), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_144452.1 |
CG13713-RA (CG13713), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_142828.1 |
CG13842-RA (CG13842), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137318.2 |
CG5859-RA (CG5859), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167294.1 |
CG2371-RC, transcript variant C (CG2371), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167293.1 |
CG2371-RA, transcript variant A (CG2371), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_132510.2 |
CG2371-RB, transcript variant B (CG2371), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137486.3 |
CG17533-RA (GstE8), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164902.1 |
CG31717-RA (CG31717), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057941.3 |
CG5227-RA, transcript variant A (sdk), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134314.2 |
CG5227-RB, transcript variant B (sdk), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134315.2 |
CG5227-RD, transcript variant D (sdk), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057942.3 |
CG5227-RC, transcript variant C (sdk), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_130498.1 |
CG13362-RA (CG13362), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140325.1 |
CG10682-RA (vih), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164741.1 |
CG4502-RB, transcript variant B (CG4502), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135259.2 |
CG4502-RA, transcript variant A (CG4502), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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