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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4842R-3 |
| Symbol |
CG4842 |
Full Name |
CG4842 |
| CG No |
CG4842 |
Old CG No |
CG4842 |
| Synonyms |
CG4842 |
| Accession No (Link to NCBI) |
NM_140628.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTACCTTGGCGGATTCGGTGGA |
| Primer Seq. 3' |
aatctagaggtaccCCTACACCAGAGTGGGCGTAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TACCTTGGCG GATTCGGTGG AATAGGCCAA AAATGTGTGC AAGAACTGCT CCAGCGACCA
0061 ATAAAGGCTC TAGCCATTTT CGATCTAAAT GCTAATGAGC AGCTCTTGGC TAAGTGGAAA
0121 AGCCAGCATC CCGATACGGA CATCTTCTAC CACAAGCTGG ACATAACCCA GAAATCGGAT
0181 ATANGACGCG GCCTACAAGG CGACCGCCGA GAGATTCGGA CACTTCGATG TGGTGGTCAA
0241 TGGCAGTGGA CTGATGAACG ATCGCCTGGT CGAACTCACT ATTCAAATTA ATCTGCTGGG
0301 AGTTATCAAC AGCACTTTGA CCGCCTTGGA GTACATGGAC AAGGCCAAAG GCGGAAAGGG
0361 TGGGCTCATA GTGAATATAT CATCGGTGGC TGGACTGCAG CCCACAGCTA TAATGGCCAT
0421 TTACTCGGCG GCCAAGACGG GAGTTACCAC ATTCACCAGA GCCATGGCTA ACCCCTACTT
0481 TTACGCCCAC TCTGGTGTAG G |
| in silico PCR Fragment |
0001 TACCTTGGCG GATTCGGTGG AATAGGCCAA AAATGTGTGC AAGAACTGCT CCAGCGACCA
0061 ATAAAGGCTC TAGCCATTTT CGATCTAAAT GCTAATGAGC AGCTCTTGGC TAAGTGGAAA
0121 AGCCAGCATC CCGATACGGA CGTCTTCTAC CACAAGCTGG ACATAACCCA GAAATCGGAT
0181 ATA-GACGCG GCCTACAAGG CGACCGCCGA GAGATTCGGA CACTTCGATG TGGTGGTCAA
0241 TGGCAGTGGA CTGATGAACG ATCGCCTGGT CGAACTCACT ATTCAAATTA ATCTGCTGGG
0301 AGTTATCAAC AGCACTTTGA CCGCCTTGGA GTACATGGAC AAGGCCAAAG GCGGAAAGGG
0361 TGGGCTCATA GTGAATATAT CATCGGTGGC TGGACTGCAG CCCACAGCTA TAATGGCCAT
0421 TTACTCGGCG GCCAAGACGG GAGTTACCAC ATTCACCAGA GCCATGGCTA ACCCCTACTT
0481 TTACGCCCAC TCTGGTGTAG G |
| Assemble Data |
<PRE>
4842R-3.IR_full 1 TACCTTGGCGGATTCGGTGGAATAGGCCAAAAATGTGTGCAAGAACTGCTCCAGCGACCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 1 TACCTTGGCGGATTCGGTGGAATAGGCCAAAAATGTGTGCAAGAACTGCTCCAGCGACCA 60
4842R-3.IR_full 61 ATAAAGGCTCTAGCCATTTTCGATCTAAATGCTAATGAGCAGCTCTTGGCTAAGTGGAAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 61 ATAAAGGCTCTAGCCATTTTCGATCTAAATGCTAATGAGCAGCTCTTGGCTAAGTGGAAA 120
4842R-3.IR_full 121 AGCCAGCATCCCGATACGGAC<span class="snp"><tt>A</tt></span>TCTTCTACCACAAGCTGGACATAACCCAGAAATCGGAT 180
|||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 121 AGCCAGCATCCCGATACGGAC<span class="snp"><tt>G</tt></span>TCTTCTACCACAAGCTGGACATAACCCAGAAATCGGAT 180
4842R-3.IR_full 181 ATA<span class="snp"><tt>N</tt></span>GACGCGGCCTACAAGGCGACCGCCGAGAGATTCGGACACTTCGATGTGGTGGTCAA 240
|||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 181 ATA<span class="snp"><tt>-</tt></span>GACGCGGCCTACAAGGCGACCGCCGAGAGATTCGGACACTTCGATGTGGTGGTCAA 240
4842R-3.IR_full 241 TGGCAGTGGACTGATGAACGATCGCCTGGTCGAACTCACTATTCAAATTAATCTGCTGGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 241 TGGCAGTGGACTGATGAACGATCGCCTGGTCGAACTCACTATTCAAATTAATCTGCTGGG 300
4842R-3.IR_full 301 AGTTATCAACAGCACTTTGACCGCCTTGGAGTACATGGACAAGGCCAAAGGCGGAAAGGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 301 AGTTATCAACAGCACTTTGACCGCCTTGGAGTACATGGACAAGGCCAAAGGCGGAAAGGG 360
4842R-3.IR_full 361 TGGGCTCATAGTGAATATATCATCGGTGGCTGGACTGCAGCCCACAGCTATAATGGCCAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 361 TGGGCTCATAGTGAATATATCATCGGTGGCTGGACTGCAGCCCACAGCTATAATGGCCAT 420
4842R-3.IR_full 421 TTACTCGGCGGCCAAGACGGGAGTTACCACATTCACCAGAGCCATGGCTAACCCCTACTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4842R-3.in silico 421 TTACTCGGCGGCCAAGACGGGAGTTACCACATTCACCAGAGCCATGGCTAACCCCTACTT 480
4842R-3.IR_full 481 TTACGCCCACTCTGGTGTAGG 501
|||||||||||||||||||||
4842R-3.in silico 481 TTACGCCCACTCTGGTGTAGG 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140628.1 |
CG4842-RA (CG4842), mRNA |
| 2.48 |
12 |
33 |
74 |
100 |
NM_144416.1 |
CG18814-RA (CG18814), mRNA |
| 0.62 |
3 |
3 |
4 |
2 |
NM_137296.2 |
CG8306-RA (CG8306), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_057425.2 |
CG9450-RA (tud), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_206146.1 |
CG9635-RF, transcript variant F (RhoGEF2), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_206147.1 |
CG9635-RE, transcript variant E (RhoGEF2), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_057969.3 |
CG9635-RD, transcript variant D (RhoGEF2), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_206636.1 |
CG3960-RF, transcript variant F (CG3960), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079427.2 |
CG6850-RA (Ugt), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_141639.1 |
CG16779-RA (CG16779), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134279.2 |
CG7734-RB, transcript variant B (shn), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057376.2 |
CG7734-RC, transcript variant C (shn), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_206091.1 |
CG7734-RD, transcript variant D (shn), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134683.1 |
CG4297-RA, transcript variant A (CG4297), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_175945.1 |
CG4297-RB, transcript variant B (CG4297), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057375.3 |
CG7734-RA, transcript variant A (shn), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_136826.1 |
CG7741-RA (CG7741), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141941.2 |
CG12276-RA (Aos1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137076.1 |
CG6701-RA (CG6701), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143462.2 |
CG1969-RA, transcript variant A (CG1969), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170422.1 |
CG1969-RB, transcript variant B (CG1969), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135876.1 |
CG4697-RA (CSN1a), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140943.2 |
CG32227-RA (CG32227), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_135391.2 |
CG13095-RA (CG13095), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_137341.1 |
CG6967-RA, transcript variant A (CG6967), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_166214.1 |
CG6967-RB, transcript variant B (CG6967), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_136000.2 |
CG6453-RA (CG6453), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_134626.1 |
CG17599-RA (CG17599), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_167525.1 |
CG9802-RB, transcript variant B (Cap), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_078650.2 |
CG9802-RA, transcript variant A (Cap), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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