NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4818R-6

Symbol

Cpr72Ea

Full Name

Cuticular protein 72Ea

CG No

CG4818

Old CG No

CG4818

Synonyms

Cpr72Ea, CG4818

Accession No (Link to NCBI)

NM_140625.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.<br> An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.<br> Zoolog Sci (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCACTTCTGCTGTTAGGAAGCC

Primer Seq. 3'

aatctagaggtaccGACGGGATGATGTCCATCTGG

Predicted Fragment Size

IR fragment full Seq

0001 CACTTCTGCT GTTAGGAAGC CTCAGCTTGG CCCACAGGAC TGGCCTTGTA CTATCCCTAT 
0061 GCATACACTG CCGAGGGATA CCGCCGTGTT CATCGCCCAC CCAGCGCCAG TATATCGCTC 
0121 AAGGATGAGT TGGGTCAGTA CTCCTATGGC TATTCGGAAC CCTTGTCCAG CAAACAGGAG 
0181 ACCCGCACCC TAGACGGAAT CACCCAAGGA TACTATAGTT ACCGGGATGC GGCGGGAAAG 
0241 CTGCAAACGG TTAACTATGT GGCCGACAAC AAAGGTTTCC ATGTGGCAGC CACCAATCTG 
0301 CCAAAGGCTA AAGTTCCCCA GGAGTCGCTA GAATTTAGTC CCAGGAGCGC GTCGCATCCA 
0361 GTAGACCACC ATGTTGAGCA TCACGCGGAG GTGTCGCATG CTGTAGTTCA GCATCCTGTT 
0421 GGCCACCATC CCATTGAAGT TCCACATCAT CACACTGTTG TTGAAAGCGG ACGAAGTGCT 
0481 CATCCAGATG GACATCATCC CGTC

in silico PCR Fragment

0001 CACTTCTGCT GTTAGGAAGC CTCAGCTTGG CCCAC-GGAC TGGCCTTGTA CTATCCCTAT 
0061 GCGTACACTG CCGAGGGAT- CCGCCGTGTT CA-CGCCCAC CCAGCGCCAG TATATCGC-C 
0121 AAGGATGAGT TGGGTCAGTA CTCCTATGGC TATTCGGAAC CATTGTCCAG CAAACAGGAG 
0181 ACCCGCACCC TAGACGGAAT CACCCAAGGA TACTATAGTT ACCGGGATGC GGCGGGAAAG 
0241 CTGCAAACGG TTAACTATGT GGCCGACAAC AAAGGTTTCC ATGTGGCAGC CACTAATCTG 
0301 CCAAAGGCTA AAGTTCCCCA GGAGTCGCTA GAATTTAGTC CCAGGAGCGC GTCGCATCCA 
0361 GTAGACCACC ATGTTGAGCA TCACGCGGAG GTGTCGCATG CTGTAGTTCA GCATCCTGTT 
0421 GGCCACCATC CCATTGAAGT TCCACATCAT CACACTGTTG TTGAAAGCGG ACGAAGTGCT 
0481 CATCCAGATG GACATCATCC CGTC

Assemble Data

<PRE>

4818R-6.IR_full       1   CACTTCTGCTGTTAGGAAGCCTCAGCTTGGCCCAC<span class="snp"><tt>A</tt></span>GGACTGGCCTTGTACTATCCCTAT 60
                          |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
4818R-6.in silico     1   CACTTCTGCTGTTAGGAAGCCTCAGCTTGGCCCAC<span class="snp"><tt>-</tt></span>GGACTGGCCTTGTACTATCCCTAT 60


4818R-6.IR_full       61  GC<span class="snp"><tt>A</tt></span>TACACTGCCGAGGGAT<span class="snp"><tt>A</tt></span>CCGCCGTGTTCA<span class="snp"><tt>T</tt></span>CGCCCACCCAGCGCCAGTATATCGC<span class="snp"><tt>T</tt></span>C 120
                          ||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
4818R-6.in silico     61  GC<span class="snp"><tt>G</tt></span>TACACTGCCGAGGGAT<span class="snp"><tt>-</tt></span>CCGCCGTGTTCA<span class="snp"><tt>-</tt></span>CGCCCACCCAGCGCCAGTATATCGC<span class="snp"><tt>-</tt></span>C 120


4818R-6.IR_full       121 AAGGATGAGTTGGGTCAGTACTCCTATGGCTATTCGGAACC<span class="snp"><tt>C</tt></span>TTGTCCAGCAAACAGGAG 180
                          |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
4818R-6.in silico     121 AAGGATGAGTTGGGTCAGTACTCCTATGGCTATTCGGAACC<span class="snp"><tt>A</tt></span>TTGTCCAGCAAACAGGAG 180


4818R-6.IR_full       181 ACCCGCACCCTAGACGGAATCACCCAAGGATACTATAGTTACCGGGATGCGGCGGGAAAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-6.in silico     181 ACCCGCACCCTAGACGGAATCACCCAAGGATACTATAGTTACCGGGATGCGGCGGGAAAG 240


4818R-6.IR_full       241 CTGCAAACGGTTAACTATGTGGCCGACAACAAAGGTTTCCATGTGGCAGCCAC<span class="snp"><tt>C</tt></span>AATCTG 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
4818R-6.in silico     241 CTGCAAACGGTTAACTATGTGGCCGACAACAAAGGTTTCCATGTGGCAGCCAC<span class="snp"><tt>T</tt></span>AATCTG 300


4818R-6.IR_full       301 CCAAAGGCTAAAGTTCCCCAGGAGTCGCTAGAATTTAGTCCCAGGAGCGCGTCGCATCCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-6.in silico     301 CCAAAGGCTAAAGTTCCCCAGGAGTCGCTAGAATTTAGTCCCAGGAGCGCGTCGCATCCA 360


4818R-6.IR_full       361 GTAGACCACCATGTTGAGCATCACGCGGAGGTGTCGCATGCTGTAGTTCAGCATCCTGTT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-6.in silico     361 GTAGACCACCATGTTGAGCATCACGCGGAGGTGTCGCATGCTGTAGTTCAGCATCCTGTT 420


4818R-6.IR_full       421 GGCCACCATCCCATTGAAGTTCCACATCATCACACTGTTGTTGAAAGCGGACGAAGTGCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-6.in silico     421 GGCCACCATCCCATTGAAGTTCCACATCATCACACTGTTGTTGAAAGCGGACGAAGTGCT 480


4818R-6.IR_full       481 CATCCAGATGGACATCATCCCGTC 504
                          ||||||||||||||||||||||||
4818R-6.in silico     481 CATCCAGATGGACATCATCCCGTC 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
104.77	505	51	51	52	NM_140625.2	CG4818-RA (CG4818), mRNA
0	0	3	2	5	NM_130635.2	CG3078-RA (CG3078), mRNA
0	0	2	4	5	NM_132678.2	CG2691-RA (CG2691), mRNA
0	0	2	4	3	NM_132488.3	CG11727-RB, transcript variant B (CG11727), mRNA
0	0	2	4	3	NM_167285.2	CG11727-RA, transcript variant A (CG11727), mRNA
0	0	2	3	3	NM_137496.1	CG5226-RA (CG5226), mRNA
0	0	2	3	2	NM_168623.1	CG7266-RC, transcript variant C (Eip71CD), mRNA
0	0	2	3	2	NM_079361.2	CG7266-RA, transcript variant A (Eip71CD), mRNA
0	0	2	3	2	NM_168622.1	CG7266-RB, transcript variant B (Eip71CD), mRNA
0	0	2	3	2	NM_206371.1	CG7266-RD, transcript variant D (Eip71CD), mRNA
0	0	2	2	3	NM_141454.1	CG10053-RA (CG10053), mRNA
0	0	2	2	3	NM_169570.1	CG31327-RA (CG31327), mRNA
0	0	1	4	3	NM_166647.2	CG3907-RA, transcript variant A (CG3907), mRNA
0	0	1	4	3	NM_138026.3	CG3907-RB, transcript variant B (CG3907), mRNA
0	0	1	2	10	NM_132412.1	CG9817-RA (CG9817), mRNA
0	0	1	2	3	NM_169436.1	CG14724-RB, transcript variant B (CoVa), mRNA
0	0	1	2	3	NR_002034.1	CG14724-RB, transcript variant B (CoVa), mRNA, miscRNA
0	0	1	2	3	NR_002033.1	CG14724-RB, transcript variant B (CoVa), mRNA, miscRNA, miscRNA
0	0	1	2	3	NR_002032.1	CG14724-RB, transcript variant B (CoVa), mRNA, miscRNA, miscRNA, miscRNA
0	0	0	5	2	NM_135028.2	CG15625-RA (CG15625), mRNA
0	0	0	4	6	NM_167470.1	CG18102-RD, transcript variant D (shi), mRNA
0	0	0	4	6	NM_206742.1	CG18102-RG, transcript variant G (shi), mRNA
0	0	0	4	6	NM_206743.1	CG18102-RF, transcript variant F (shi), mRNA
0	0	0	4	6	NM_140948.2	CG6680-RA, transcript variant A (CG6680), mRNA
0	0	0	4	6	NM_168844.1	CG6680-RB, transcript variant B (CG6680), mRNA
0	0	0	4	4	NM_079549.2	CG7850-RA (puc), mRNA
0	0	0	4	3	NM_142755.1	CG5732-RA (CG5732), mRNA
0	0	0	4	3	NM_136397.1	CG15236-RA, transcript variant A (CG15236), mRNA
0	0	0	4	3	NM_165493.1	CG15236-RB, transcript variant B (CG15236), mRNA
0	0	0	4	2	NM_057841.3	CG5102-RA (da), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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