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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4818R-3 |
| Symbol |
Cpr72Ea |
Full Name |
Cuticular protein 72Ea |
| CG No |
CG4818 |
Old CG No |
CG4818 |
| Synonyms |
Cpr72Ea, CG4818 |
| Accession No (Link to NCBI) |
NM_140625.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.<br> An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.<br> Zoolog Sci (2016) 33(6) 583-591
[
PubMed ID = 27927092
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACTTCTGCTGTTAGGAAGCC |
| Primer Seq. 3' |
aatctagaggtaccGACGGGATGATGTCCATCTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACTTCTGCT GTTAGGAAGC CTCAGCTTGG CCCACAGGAC TGGCCTTGTA CTATCCCTAT
0061 GCATACACTG CCGAGGGATA CCGCCGTGTT CATCGCCCAC CCAGCGCCAG TATATCGCTC
0121 AAGGATGAGT TGGGTCAGTA CTCCTATGGC TATTCGGAAC CCTTGTCCAG CAAACAGGAG
0181 ACCCGCACCC TAGACGGAAT CACCCAAGGA TACTATAGTT ACCGGGATGC GGCGGGAAAG
0241 CTGCAAACGG TTAACTATGT GGCCGACAAC AAAGGTTTCC ATGTGGCAGC CACCAATCTG
0301 CCAAAGGCTA AAGTTCCCCA GGAGTCGCTA GAATTTAGTC CCAGGAGCGC GTCGCATCCA
0361 GTAGACCACC ATGTTGAGCA TCACGCGGAG GTGTCGCATG CTGTAGTTCA GCATCCTGTT
0421 GGCCACCATC CCATTGAAGT TCCACATCAT CACACTGTTG TTGAAAGCGG ACGAAGTGCT
0481 CATCCAGATG GACATCATCC CGTC |
| in silico PCR Fragment |
0001 CACTTCTGCT GTTAGGAAGC CTCAGCTTGG CCCAC-GGAC TGGCCTTGTA CTATCCCTAT
0061 GCGTACACTG CCGAGGGAT- CCGCCGTGTT CA-CGCCCAC CCAGCGCCAG TATATCGC-C
0121 AAGGATGAGT TGGGTCAGTA CTCCTATGGC TATTCGGAAC CATTGTCCAG CAAACAGGAG
0181 ACCCGCACCC TAGACGGAAT CACCCAAGGA TACTATAGTT ACCGGGATGC GGCGGGAAAG
0241 CTGCAAACGG TTAACTATGT GGCCGACAAC AAAGGTTTCC ATGTGGCAGC CACTAATCTG
0301 CCAAAGGCTA AAGTTCCCCA GGAGTCGCTA GAATTTAGTC CCAGGAGCGC GTCGCATCCA
0361 GTAGACCACC ATGTTGAGCA TCACGCGGAG GTGTCGCATG CTGTAGTTCA GCATCCTGTT
0421 GGCCACCATC CCATTGAAGT TCCACATCAT CACACTGTTG TTGAAAGCGG ACGAAGTGCT
0481 CATCCAGATG GACATCATCC CGTC |
| Assemble Data |
<PRE>
4818R-3.IR_full 1 CACTTCTGCTGTTAGGAAGCCTCAGCTTGGCCCAC<span class="snp"><tt>A</tt></span>GGACTGGCCTTGTACTATCCCTAT 60
|||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||
4818R-3.in silico 1 CACTTCTGCTGTTAGGAAGCCTCAGCTTGGCCCAC<span class="snp"><tt>-</tt></span>GGACTGGCCTTGTACTATCCCTAT 60
4818R-3.IR_full 61 GC<span class="snp"><tt>A</tt></span>TACACTGCCGAGGGAT<span class="snp"><tt>A</tt></span>CCGCCGTGTTCA<span class="snp"><tt>T</tt></span>CGCCCACCCAGCGCCAGTATATCGC<span class="snp"><tt>T</tt></span>C 120
||<span class="snp"><tt> </tt></span>||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|
4818R-3.in silico 61 GC<span class="snp"><tt>G</tt></span>TACACTGCCGAGGGAT<span class="snp"><tt>-</tt></span>CCGCCGTGTTCA<span class="snp"><tt>-</tt></span>CGCCCACCCAGCGCCAGTATATCGC<span class="snp"><tt>-</tt></span>C 120
4818R-3.IR_full 121 AAGGATGAGTTGGGTCAGTACTCCTATGGCTATTCGGAACC<span class="snp"><tt>C</tt></span>TTGTCCAGCAAACAGGAG 180
|||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||
4818R-3.in silico 121 AAGGATGAGTTGGGTCAGTACTCCTATGGCTATTCGGAACC<span class="snp"><tt>A</tt></span>TTGTCCAGCAAACAGGAG 180
4818R-3.IR_full 181 ACCCGCACCCTAGACGGAATCACCCAAGGATACTATAGTTACCGGGATGCGGCGGGAAAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-3.in silico 181 ACCCGCACCCTAGACGGAATCACCCAAGGATACTATAGTTACCGGGATGCGGCGGGAAAG 240
4818R-3.IR_full 241 CTGCAAACGGTTAACTATGTGGCCGACAACAAAGGTTTCCATGTGGCAGCCAC<span class="snp"><tt>C</tt></span>AATCTG 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||
4818R-3.in silico 241 CTGCAAACGGTTAACTATGTGGCCGACAACAAAGGTTTCCATGTGGCAGCCAC<span class="snp"><tt>T</tt></span>AATCTG 300
4818R-3.IR_full 301 CCAAAGGCTAAAGTTCCCCAGGAGTCGCTAGAATTTAGTCCCAGGAGCGCGTCGCATCCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-3.in silico 301 CCAAAGGCTAAAGTTCCCCAGGAGTCGCTAGAATTTAGTCCCAGGAGCGCGTCGCATCCA 360
4818R-3.IR_full 361 GTAGACCACCATGTTGAGCATCACGCGGAGGTGTCGCATGCTGTAGTTCAGCATCCTGTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-3.in silico 361 GTAGACCACCATGTTGAGCATCACGCGGAGGTGTCGCATGCTGTAGTTCAGCATCCTGTT 420
4818R-3.IR_full 421 GGCCACCATCCCATTGAAGTTCCACATCATCACACTGTTGTTGAAAGCGGACGAAGTGCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4818R-3.in silico 421 GGCCACCATCCCATTGAAGTTCCACATCATCACACTGTTGTTGAAAGCGGACGAAGTGCT 480
4818R-3.IR_full 481 CATCCAGATGGACATCATCCCGTC 504
||||||||||||||||||||||||
4818R-3.in silico 481 CATCCAGATGGACATCATCCCGTC 504
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 104.77 |
505 |
51 |
51 |
52 |
NM_140625.2 |
CG4818-RA (CG4818), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_130635.2 |
CG3078-RA (CG3078), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_132678.2 |
CG2691-RA (CG2691), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_132488.3 |
CG11727-RB, transcript variant B (CG11727), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_167285.2 |
CG11727-RA, transcript variant A (CG11727), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137496.1 |
CG5226-RA (CG5226), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_168623.1 |
CG7266-RC, transcript variant C (Eip71CD), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079361.2 |
CG7266-RA, transcript variant A (Eip71CD), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_168622.1 |
CG7266-RB, transcript variant B (Eip71CD), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206371.1 |
CG7266-RD, transcript variant D (Eip71CD), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141454.1 |
CG10053-RA (CG10053), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169570.1 |
CG31327-RA (CG31327), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_166647.2 |
CG3907-RA, transcript variant A (CG3907), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_138026.3 |
CG3907-RB, transcript variant B (CG3907), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169436.1 |
CG14724-RB, transcript variant B (CoVa), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NR_002034.1 |
CG14724-RB, transcript variant B (CoVa), mRNA, miscRNA |
| 0 |
0 |
1 |
2 |
3 |
NR_002033.1 |
CG14724-RB, transcript variant B (CoVa), mRNA, miscRNA, miscRNA |
| 0 |
0 |
1 |
2 |
3 |
NR_002032.1 |
CG14724-RB, transcript variant B (CoVa), mRNA, miscRNA, miscRNA, miscRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_135028.2 |
CG15625-RA (CG15625), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_167470.1 |
CG18102-RD, transcript variant D (shi), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_206742.1 |
CG18102-RG, transcript variant G (shi), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_206743.1 |
CG18102-RF, transcript variant F (shi), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_140948.2 |
CG6680-RA, transcript variant A (CG6680), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168844.1 |
CG6680-RB, transcript variant B (CG6680), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079549.2 |
CG7850-RA (puc), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142755.1 |
CG5732-RA (CG5732), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_136397.1 |
CG15236-RA, transcript variant A (CG15236), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_165493.1 |
CG15236-RB, transcript variant B (CG15236), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_057841.3 |
CG5102-RA (da), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
|
| Dcompartment |
|
Penetrance |
| |
|
- |
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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