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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4689R-1 
 Symbol CG4877  Full Name CG4877 
 CG No CG4877  Old CG No CG4689 
 Synonyms CG4689, CG4877 
 Accession No (Link to NCBI) NM_168664.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATGGCCCAGAAAGAGGATGA 
 Primer Seq. 3' 
aatctagaggtaccGGTTACTGTCTTGCGAGTGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NATGNCCCAG AAAGAGGATG AATATAACCG ACTGCTGTAT TTGAGCTGCA TTAAGAAGGA 
0061 GCTGCATGAA CGAATGGAGC GAAAGGAGCG CTTGATACGT ATTAAGGACC TACTGCCCAT 
0121 CCTTATCAAC AAGTGCGGCA CCAAGGAGCT CAGTGAGGTA AAGGCTATGC CTAGAAGAGG 
0181 AAATCGATTC TCCAATGGCC TCCAAACATA GTTTGTCGGC AATTGAAAAA TTACTGAATT 
0241 ACGCCGAGTT AAACCAGAAC ATCATACAGC TTCTGCGGGG TTCCTTGGGC TTAAATAAAC 
0301 GCGCACCGTC CGTGTCTCCG GCCAATTTCG AGGATGACCA AATGCTTTTT CGTAGAGATT 
0361 CCTTGCCCGT AAGTCATCTA TCAATGCGGG ATCAGCATTA CGAATCTCGG AATGCGGTAA 
0421 ATGACCATCT GCTAGATAGC AATCCAGACA GAGATCCGCC AGCCAAGCGA CCGAAGCTAA 
0481 TCCACTCGCA AGACAGTAAC C 
 in silico PCR Fragment 
0001 GATGGCCCAG AAAGAGGATG AATATAACCG ACTGCTGTAT TTGAGCTGCA TTAAGAAGGA 
0061 GCTGCATGAA CGAATGGAGC GAAAGGAGCG CTTGATACGT ATTAAGGACC TACTGCCCAT 
0121 CCTTATCAAC AAGTGCGGCA CCAAGGAGCT CAGTGAGGTA AAGGCTATG- CTAGAAGAGG 
0181 AAATCGATTC TCCAATGGCC TCCAAACATA GTTTGTCGGC AATTGAAAAA TTACTGAATT 
0241 ACGCCGAGTT AAACCAGAAC ATCATACAGC TTCTGCGGGG TTCCTTGGGC TTAAATAAAC 
0301 GCGCACCGTC CGTGTCTCCG GCCAATTTCG AGGATGACCA AATGCTTTTT CGTAGAGATT 
0361 CCTTGCCCGT AAGTCATCTA TCAATGCGGG ATCAGCATTA CGAATCTCGG AATGCGGTAA 
0421 ATGACCATCT GCTAGATAGC AATCCAGACA GAGATCCGCC AGCCAAGCGA CCGAAGCTAA 
0481 TCCACTCGCA AGACAGTAAC C 
 Assemble Data 
<PRE>

4689R-1.IR_full        1   ATG<span class="snp"><tt>N</tt></span>CCCAGAAAGAGGATGAATATAACCGACTGCTGTATTTGAGCTGCATTAAGAAGGA 59
                           |||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
4689R-1.in silico      1   ATG<span class="snp"><tt>G</tt></span>CCCAGAAAGAGGATGAATATAACCGACTGCTGTATTTGAGCTGCATTAAGAAGGA 59


4689R-1.IR_full       61  GCTGCATGAACGAATGGAGCGAAAGGAGCGCTTGATACGTATTAAGGACCTACTGCCCAT 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4689R-1.in silico     61  GCTGCATGAACGAATGGAGCGAAAGGAGCGCTTGATACGTATTAAGGACCTACTGCCCAT 119


4689R-1.IR_full       121 CCTTATCAACAAGTGCGGCACCAAGGAGCTCAGTGAGGTAAAGGCTATG<span class="snp"><tt>C</tt></span>CTAGAAGAGG 179
                          |||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||
4689R-1.in silico     121 CCTTATCAACAAGTGCGGCACCAAGGAGCTCAGTGAGGTAAAGGCTATG<span class="snp"><tt>-</tt></span>CTAGAAGAGG 179


4689R-1.IR_full       181 AAATCGATTCTCCAATGGCCTCCAAACATAGTTTGTCGGCAATTGAAAAATTACTGAATT 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4689R-1.in silico     181 AAATCGATTCTCCAATGGCCTCCAAACATAGTTTGTCGGCAATTGAAAAATTACTGAATT 239


4689R-1.IR_full       241 ACGCCGAGTTAAACCAGAACATCATACAGCTTCTGCGGGGTTCCTTGGGCTTAAATAAAC 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4689R-1.in silico     241 ACGCCGAGTTAAACCAGAACATCATACAGCTTCTGCGGGGTTCCTTGGGCTTAAATAAAC 299


4689R-1.IR_full       301 GCGCACCGTCCGTGTCTCCGGCCAATTTCGAGGATGACCAAATGCTTTTTCGTAGAGATT 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4689R-1.in silico     301 GCGCACCGTCCGTGTCTCCGGCCAATTTCGAGGATGACCAAATGCTTTTTCGTAGAGATT 359


4689R-1.IR_full       361 CCTTGCCCGTAAGTCATCTATCAATGCGGGATCAGCATTACGAATCTCGGAATGCGGTAA 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4689R-1.in silico     361 CCTTGCCCGTAAGTCATCTATCAATGCGGGATCAGCATTACGAATCTCGGAATGCGGTAA 419


4689R-1.IR_full       421 ATGACCATCTGCTAGATAGCAATCCAGACAGAGATCCGCCAGCCAAGCGACCGAAGCTAA 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4689R-1.in silico     421 ATGACCATCTGCTAGATAGCAATCCAGACAGAGATCCGCCAGCCAAGCGACCGAAGCTAA 479


4689R-1.IR_full       481 TCCACTCGCAAGACAGTAACC 500
                          |||||||||||||||||||||
4689R-1.in silico     481 TCCACTCGCAAGACAGTAACC 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140632.2  CG4877-RB, transcript variant B (CG4877), mRNA 
100   482  0  0  0  NM_168664.1  CG4877-RA, transcript variant A (CG4877), mRNA 
0   0  4  2  3  NM_140213.3  CG7533-RC (chrb), mRNA 
0   0  2  3  3  NM_137013.2  CG4714-RA (CG4714), mRNA 
0   0  1  3  6  NM_134735.1  CG4785-RA (CG4785), mRNA 
0   0  1  2  5  NM_132762.3  CG32594-RB, transcript variant B (CG32594), mRNA 
0   0  1  2  5  NM_167424.1  CG32594-RC, transcript variant C (CG32594), mRNA 
0   0  1  2  3  NM_135996.2  CG6639-RA (CG6639), mRNA 
0   0  0  5  5  NM_135122.1  CG9029-RA (CG9029), mRNA 
0   0  0  4  6  NM_135651.2  CG4751-RA (CG4751), mRNA 
0   0  0  4  4  NM_135550.1  CG5362-RA (CG5362), mRNA 
0   0  0  4  4  NM_168690.1  CG13029-RB, transcript variant B (CG13029), mRNA 
0   0  0  4  2  NM_136955.2  CG8815-RA, transcript variant A (Sin3A), mRNA 
0   0  0  4  2  NM_165916.1  CG8815-RC, transcript variant C (Sin3A), mRNA 
0   0  0  4  2  NM_165915.1  CG8815-RB, transcript variant B (Sin3A), mRNA 
0   0  0  4  2  NM_165917.1  CG8815-RD, transcript variant D (Sin3A), mRNA 
0   0  0  3  8  NM_132551.2  CG1490-RB (Usp7), mRNA 
0   0  0  3  7  NM_163886.1  CG32491-RP, transcript variant P (mod(mdg4)), mRNA 
0   0  0  3  5  NM_135301.2  CG7196-RA, transcript variant A (CG7196), mRNA 
0   0  0  3  5  NM_140937.2  CG17149-RA, transcript variant A (CG17149), mRNA 
0   0  0  3  5  NM_168835.1  CG17149-RB, transcript variant B (CG17149), mRNA 
0   0  0  3  5  NM_142550.1  CG3517-RA (CG3517), mRNA 
0   0  0  3  4  NM_206162.1  CG5580-RC, transcript variant C (sbb), mRNA 
0   0  0  3  4  NM_170639.1  CG17122-RA (CG17122), mRNA 
0   0  0  3  4  NM_139418.1  CG1887-RA (CG1887), mRNA 
0   0  0  3  4  NM_079939.2  CG5580-RA, transcript variant A (sbb), mRNA 
0   0  0  3  4  NM_167312.1  CG10353-RB, transcript variant B (CG10353), mRNA 
0   0  0  3  4  NM_139938.2  CG7213-RA (CG7213), mRNA 
0   0  0  3  4  NM_206161.1  CG5580-RB, transcript variant B (sbb), mRNA 
0   0  0  3  3  NM_079055.2  CG4943-RA (lack), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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