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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4684R-1 |
| Symbol |
nwk |
Full Name |
nervous wreck |
| CG No |
CG4684 |
Old CG No |
CG4684 |
| Synonyms |
CG4684, nwk |
| Accession No (Link to NCBI) |
NM_140033.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
2 lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCGCCGCGTAAGGGAAACTAT |
| Primer Seq. 3' |
aatctagaggtaccTCCCGAACATCGTGAGCACAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCGCCGCGTA AGGGAAACTA TGTGAAATTC CTCAAGAATT TGCACACGGA GCAGGTGGCC
0061 AAGCTGCAGC TGAAGAATCA GCATGAATGC GACCTCCTGG AGGACATCCG GCAGTTCACC
0121 ATNCAAGCGT TCCGCTGTCG AGAAGTCATA CAGTGAGTCG CTGCTVAAGA TCTCATCGCA
0181 GTATCTCAAC AAGAAGATAC CCAATATACC AGATATTAAG ATGGAGGGCA TGGAGGAACG
0241 CTTGGAACAT GTGGAGCGTT TGGCGCACTG TGCTCGAGGA GAATGAGAAA CTGGCTCGTG
0301 CTCGATTGGC CGCCATAGAG GTGTTTCAAC AGCAAATCGC GGATGAGGCC AAGGTTCTGC
0361 GGGACTATAA GTTGGCCATA GCTNAGCGTT CGCTAGCCGG AATTGTCAAT GTGCAGAAGG
0421 AACTCCATCT GAGCGTGGGC GATGTGGATA AGACCAAGAA ATCATACTTT GACGAAGAGC
0481 ATTGTGCTCA CGATGTTCGG GA |
| in silico PCR Fragment |
0001 CCGCCGCGTA AGGGAAACTA TGTGAAATTC CTCAAGAATT TGCACACGGA GCAGGTGGCC
0061 AAGCTGCAGC TGAAGAATCA GCATGAATGC GACCTCCTGG AGGACATCCG GCAGTTCACC
0121 AT-CAAGCGT TCCGCTGTCG AGAAGTCATA CAGTGAGTCG CTGCTGAAGA TCTCATCGCA
0181 GTATCTCAAC AAGAAGATAC CCAATATACC AGATATTAAG ATGGAGGGCA TGGAGGAACG
0241 C-TGGAACAT GTGGAGCGTT TGGCGCACTG TGCTCGAGGA GAATGAGAAA CTGGCTCGTG
0301 CTCGATTGGC CGCCATAGAG GTGTTTCAAC AGCAAATCGC GGATGAGGCC AAGGTTCTGC
0361 GGGACTATAA GTTGGCCATA GCTAAGCGTT CGCTAGCCGG AATTGTCAAT GTGCAGAAGG
0421 AACTCCATCT GAGCGTGGGC GATGTGGATA AGACCAAGAA ATCATACTTT GACGAAGAGC
0481 ATTGTGCTCA CGATGTTCGG GA |
| Assemble Data |
<PRE>
4684R-1.IR_full 1 CCGCCGCGTAAGGGAAACTATGTGAAATTCCTCAAGAATTTGCACACGGAGCAGGTGGCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4684R-1.in silico 1 CCGCCGCGTAAGGGAAACTATGTGAAATTCCTCAAGAATTTGCACACGGAGCAGGTGGCC 60
4684R-1.IR_full 61 AAGCTGCAGCTGAAGAATCAGCATGAATGCGACCTCCTGGAGGACATCCGGCAGTTCACC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4684R-1.in silico 61 AAGCTGCAGCTGAAGAATCAGCATGAATGCGACCTCCTGGAGGACATCCGGCAGTTCACC 120
4684R-1.IR_full 121 AT<span class="snp"><tt>N</tt></span>CAAGCGTTCCGCTGTCGAGAAGTCATACAGTGAGTCGCTGCT<span class="snp"><tt>V</tt></span>AAGATCTCATCGCA 180
||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||
4684R-1.in silico 121 AT<span class="snp"><tt>-</tt></span>CAAGCGTTCCGCTGTCGAGAAGTCATACAGTGAGTCGCTGCT<span class="snp"><tt>G</tt></span>AAGATCTCATCGCA 180
4684R-1.IR_full 181 GTATCTCAACAAGAAGATACCCAATATACCAGATATTAAGATGGAGGGCATGGAGGAACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4684R-1.in silico 181 GTATCTCAACAAGAAGATACCCAATATACCAGATATTAAGATGGAGGGCATGGAGGAACG 240
4684R-1.IR_full 241 C<span class="snp"><tt>T</tt></span>TGGAACATGTGGAGCGTTTGGCGCACTGTGCTCGAGGAGAATGAGAAACTGGCTCGTG 300
|<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4684R-1.in silico 241 C<span class="snp"><tt>-</tt></span>TGGAACATGTGGAGCGTTTGGCGCACTGTGCTCGAGGAGAATGAGAAACTGGCTCGTG 300
4684R-1.IR_full 301 CTCGATTGGCCGCCATAGAGGTGTTTCAACAGCAAATCGCGGATGAGGCCAAGGTTCTGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4684R-1.in silico 301 CTCGATTGGCCGCCATAGAGGTGTTTCAACAGCAAATCGCGGATGAGGCCAAGGTTCTGC 360
4684R-1.IR_full 361 GGGACTATAAGTTGGCCATAGCT<span class="snp"><tt>N</tt></span>AGCGTTCGCTAGCCGGAATTGTCAATGTGCAGAAGG 420
|||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||
4684R-1.in silico 361 GGGACTATAAGTTGGCCATAGCT<span class="snp"><tt>A</tt></span>AGCGTTCGCTAGCCGGAATTGTCAATGTGCAGAAGG 420
4684R-1.IR_full 421 AACTCCATCTGAGCGTGGGCGATGTGGATAAGACCAAGAAATCATACTTTGACGAAGAGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4684R-1.in silico 421 AACTCCATCTGAGCGTGGGCGATGTGGATAAGACCAAGAAATCATACTTTGACGAAGAGC 480
4684R-1.IR_full 481 ATTGTGCTCACGATGTTCGGGA 502
||||||||||||||||||||||
4684R-1.in silico 481 ATTGTGCTCACGATGTTCGGGA 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140033.1 |
CG4684-RA (nwk), mRNA |
| 0 |
0 |
5 |
4 |
6 |
NM_169700.2 |
CG31045-RA, transcript variant A (Mhcl), mRNA |
| 0 |
0 |
5 |
4 |
6 |
NM_169703.1 |
CG31045-RD, transcript variant D (Mhcl), mRNA |
| 0 |
0 |
5 |
4 |
6 |
NM_001043250.1 |
CG31045-RG, transcript variant G (Mhcl), mRNA |
| 0 |
0 |
5 |
4 |
6 |
NM_001043251.1 |
CG31045-RF, transcript variant F (Mhcl), mRNA |
| 0 |
0 |
5 |
4 |
6 |
NM_170650.3 |
CG31045-RC, transcript variant C (Mhcl), mRNA |
| 0 |
0 |
5 |
4 |
6 |
NM_169701.2 |
CG31045-RB, transcript variant B (Mhcl), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_143437.2 |
CG2310-RA, transcript variant A (CG2310), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_170411.1 |
CG2310-RB, transcript variant B (CG2310), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_135100.2 |
CG7251-RA (CG7251), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_140424.1 |
CG9007-RA (CG9007), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_057864.3 |
CG9127-RA, transcript variant A (ade2), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_164675.1 |
CG9127-RB, transcript variant B (ade2), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_164676.1 |
CG9127-RC, transcript variant C (ade2), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_132133.1 |
CG3044-RA (CG3044), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_135968.1 |
CG13280-RA (CG13280), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_170365.1 |
CG14066-RC, transcript variant C (larp), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_170366.1 |
CG14066-RB, transcript variant B (larp), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_080259.1 |
CG14066-RA, transcript variant A (larp), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_139601.2 |
CG14991-RB, transcript variant B (Fit1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_168060.1 |
CG14991-RA, transcript variant A (Fit1), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_140872.1 |
CG9330-RA (CG9330), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_143464.2 |
CG1972-RA (CG1972), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_166474.1 |
CG9878-RB, transcript variant B (Tim10), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_137762.2 |
CG9878-RA, transcript variant A (Tim10), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141186.3 |
CG14639-RA (CG14639), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_057962.4 |
CG1782-RA (Uba1), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_132339.2 |
CG32701-RA (l(1)G0320), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_135841.4 |
CG8954-RA, transcript variant A (Smg5), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_139511.2 |
CG1869-RA (CG1869), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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