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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4680R-3

Symbol

CG4680

Full Name

CG4680

CG No

CG4680

Old CG No

CG4680

Synonyms

CG4680

Accession No (Link to NCBI)

NM_140634.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTACAATCAGGTCCTTAGCCGT

Primer Seq. 3'

aatctagaggtaccAGATGATCTCGGAACACCTGC

Predicted Fragment Size

IR fragment full Seq

0001 TACAATCAGG TCCTTAGCCG TCTGAAGCTA GTGGAGAACT TCGATGGCAG TGATCCTGGC 
0061 AAGCTGCCGC AGTTCCTGCA CAAAATCGAG GCCCTTATGC CGGCCATAAA TAGTTTCGAC 
0121 GACTACTACA AGTTTCAATT GATAGGACAT ATCAAAAATA AGTGCACAGA TCGCGCCAGG 
0181 GAGGcGGTCT NTCACGCGAC AAATGAGCGC TCCCGTGGGC AATGGCAATG GCAATGGCGG 
0241 CGGGAATGGT CATCTCCTGA AGGCCGAGTC CTGGCTAAAA CTCAAGGATC AACTGCTGTA 
0301 CAACTATGCG GAACGGGAGT CCAGCTACTC GCTGATACAC AAGATACGCA GCGCTGTCTT 
0361 GGACTCGAAC ATAGAGTTGT ACTACCAGAA GATGGCCAAA CTCAAGCACA GACTGATCAA 
0421 TAGAAAGCTA ACGCACAATG ACACCCTGTA CAGTCTCGAG GACATAGAGC GCATCACGCT 
0481 GCAGGTGTTC CGAGTNATCA TCT

in silico PCR Fragment

0001 TACAATCAGG TCCTTAGCCG TCTGAAGCTA GTGGAGAACT TCGATGGCAG TGATCCTGGC 
0061 AAGCTGCCGC AGTTCCTGCA CAAAATCGAG GCCCTTATGC CGGCCATAAA TAGTTTCGAC 
0121 GACTACTACA AGTTTCAATT GATAGGACAT ATCAAAAATA AGTGCACAGA TCGCGCCAGG 
0181 GAGGCGGTCT -TCACGCGAC AAATGAGCGC TCCCGTGGGC AATGGCAATG GCAATGGCGG 
0241 CGGGAATGGT CATCTCCTGA AGGCCGAGTC CTGGCTAAAA CTCAAGGATC AACTGCTGTA 
0301 CAACTATGCG GAGCGGGAGT CTAGCTACTC GCTGATACAC AAGATACGCA GCGCTGTCTT 
0361 GGACTCGAAC ATAGAGTTGT ACTACCAGAA GATGGCCAAA CTCAAGCACA GACTGATCAA 
0421 TAGAAAGCTC ACGCACAATG ACACCCTGTA CAGTCTCGAG GACATAGAGC GCATCACGCT 
0481 GCAGGTGTTC CGAG--ATCA TCT

Assemble Data

<PRE>

4680R-3.IR_full       1   TACAATCAGGTCCTTAGCCGTCTGAAGCTAGTGGAGAACTTCGATGGCAGTGATCCTGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     1   TACAATCAGGTCCTTAGCCGTCTGAAGCTAGTGGAGAACTTCGATGGCAGTGATCCTGGC 60


4680R-3.IR_full       61  AAGCTGCCGCAGTTCCTGCACAAAATCGAGGCCCTTATGCCGGCCATAAATAGTTTCGAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     61  AAGCTGCCGCAGTTCCTGCACAAAATCGAGGCCCTTATGCCGGCCATAAATAGTTTCGAC 120


4680R-3.IR_full       121 GACTACTACAAGTTTCAATTGATAGGACATATCAAAAATAAGTGCACAGATCGCGCCAGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     121 GACTACTACAAGTTTCAATTGATAGGACATATCAAAAATAAGTGCACAGATCGCGCCAGG 180


4680R-3.IR_full       181 GAGGCGGTCT<span class="snp"><tt>N</tt></span>TCACGCGACAAATGAGCGCTCCCGTGGGCAATGGCAATGGCAATGGCGG 240
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     181 GAGGCGGTCT<span class="snp"><tt>-</tt></span>TCACGCGACAAATGAGCGCTCCCGTGGGCAATGGCAATGGCAATGGCGG 240


4680R-3.IR_full       241 CGGGAATGGTCATCTCCTGAAGGCCGAGTCCTGGCTAAAACTCAAGGATCAACTGCTGTA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     241 CGGGAATGGTCATCTCCTGAAGGCCGAGTCCTGGCTAAAACTCAAGGATCAACTGCTGTA 300


4680R-3.IR_full       301 CAACTATGCGGA<span class="snp"><tt>A</tt></span>CGGGAGTC<span class="snp"><tt>C</tt></span>AGCTACTCGCTGATACACAAGATACGCAGCGCTGTCTT 360
                          ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     301 CAACTATGCGGA<span class="snp"><tt>G</tt></span>CGGGAGTC<span class="snp"><tt>T</tt></span>AGCTACTCGCTGATACACAAGATACGCAGCGCTGTCTT 360


4680R-3.IR_full       361 GGACTCGAACATAGAGTTGTACTACCAGAAGATGGCCAAACTCAAGCACAGACTGATCAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     361 GGACTCGAACATAGAGTTGTACTACCAGAAGATGGCCAAACTCAAGCACAGACTGATCAA 420


4680R-3.IR_full       421 TAGAAAGCT<span class="snp"><tt>A</tt></span>ACGCACAATGACACCCTGTACAGTCTCGAGGACATAGAGCGCATCACGCT 480
                          |||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
4680R-3.in silico     421 TAGAAAGCT<span class="snp"><tt>C</tt></span>ACGCACAATGACACCCTGTACAGTCTCGAGGACATAGAGCGCATCACGCT 480


4680R-3.IR_full       481 GCAGGTGTTCCGAG<span class="snp"><tt>T</tt></span><span class="snp"><tt>N</tt></span>ATCATCT 503
                          ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||
4680R-3.in silico     481 GCAGGTGTTCCGAG<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>ATCATCT 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	6	NM_140634.1	CG4680-RA (CG4680), mRNA
0.62	3	2	11	13	NM_137194.1	CG8179-RA (CG8179), mRNA
0.41	2	4	2	5	NM_166555.1	CG3510-RA, transcript variant A (CycB), mRNA
0.41	2	4	2	5	NM_166556.1	CG3510-RD, transcript variant D (CycB), mRNA
0.41	2	4	2	5	NM_166557.1	CG3510-RB, transcript variant B (CycB), mRNA
0.41	2	4	2	5	NM_166558.1	CG3510-RC, transcript variant C (CycB), mRNA
0.41	2	3	5	15	NM_142105.1	CG14842-RA (CG14842), mRNA
0.2	1	3	6	8	NM_206678.1	CG33175-RA, transcript variant A (spri), mRNA
0.2	1	3	6	8	NM_176723.2	CG33175-RG, transcript variant G (spri), mRNA
0.2	1	3	6	8	NM_176724.1	CG33175-RH, transcript variant H (spri), mRNA
0.2	1	2	2	8	NM_078689.2	CG14228-RA (Mer), mRNA
0	0	6	6	15	NM_141280.2	CG14669-RA (CG14669), mRNA
0	0	5	12	18	NM_136857.2	CG12443-RA (ths), mRNA
0	0	5	8	8	NM_166897.1	CG11491-RC, transcript variant C (br), mRNA
0	0	5	8	8	NM_166894.1	CG11491-RA, transcript variant A (br), mRNA
0	0	5	8	8	NM_166895.1	CG11491-RD, transcript variant D (br), mRNA
0	0	5	8	8	NM_166896.1	CG11491-RB, transcript variant B (br), mRNA
0	0	5	8	8	NM_080020.3	CG11491-RE, transcript variant E (br), mRNA
0	0	5	8	8	NM_166898.1	CG11491-RG, transcript variant G (br), mRNA
0	0	5	8	8	NM_166893.1	CG11491-RF, transcript variant F (br), mRNA
0	0	5	5	6	NM_001038946.1	CG33969-RB, transcript variant B (CG33969), mRNA
0	0	5	5	6	NM_168852.2	CG5725-RD, transcript variant D (fbl), mRNA
0	0	5	5	6	NM_168851.2	CG5725-RC, transcript variant C (fbl), mRNA
0	0	5	5	6	NM_133434.3	CG5725-RB, transcript variant B (fbl), mRNA
0	0	5	5	6	NM_080131.4	CG5725-RE, transcript variant E (fbl), mRNA
0	0	5	5	6	NM_138997.2	CG5725-RA, transcript variant A (fbl), mRNA
0	0	5	3	6	NM_140050.2	CG4022-RA (CG4022), mRNA
0	0	4	12	14	NM_136397.1	CG15236-RA, transcript variant A (CG15236), mRNA
0	0	4	12	14	NM_165493.1	CG15236-RB, transcript variant B (CG15236), mRNA
0	0	4	7	9	NM_176106.1	CG33141-RA, transcript variant A (sns), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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