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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4672R-3 
 Symbol TMS1  Full Name TMS1 
 CG No CG4672  Old CG No CG4672 
 Synonyms TMS1d, CG4672, TMS1 
 Accession No (Link to NCBI) NM_140636.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment balanced with Green Balancer. CyO, P{w[+mC]=ActGFP}JMR1 
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGCGCTTCATGTACGCCTTCA 
 Primer Seq. 3' 
aatctagaggtaccACGACTGTTTTCGGCGCTTTC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGCGCTTCAT GTACGCCTTC ATCCTCCTGG TGGGCACCGT ACTGGGGGCG ATTGCGCTGT 
0061 CCCCTGGACT GCAGNACACC CTGAAGAAGA TGCCCTTCTG CATCAACTCC ACCTCGTCGT 
0121 ATAGCTCGGG AGCACTCAGC GCTGTCTCGG GAGGATCTCT GCAAGTCGAC TGCGAGTATG 
0181 CACTTGGTTA CATGGCAGTA TATAGGGTTT GCTTTGGCAT GGCCTGCTTT TTTCGCTCTG 
0241 ATGTCACTGA TTATGCTGGG TGTGAAGAGC TCCANGGGAT CCGCGTTCGC ACATCCAGAA 
0301 CAACTTTTGG CCACTGAAAT TTCTGATCCT GCTTTGGNGC TGCGATTGGA GCCATCTTTA 
0361 TTCCGGATGG ATCCTTTGGT CCGGCCATGA TGTGGGTGGG TCTAATTGGT gGCCTGGCCT 
0421 TCATACTCGT CCAGCTGGTC ATCATTGTGG ATTTCGCTCA CTCGCTGGCT GAAAACTGGA 
0481 TAGAAAGCGC CGAAAACAGT CGT 
 in silico PCR Fragment 
0001 CGCGCTTCAT GTACGCCTTC ATCCTCCTGG TGGGCACCGT ACTGGGGGCG ATTGCGCTGT 
0061 CCCCTGGACT GCAGGACACC CTGAAGAAGA TGCCCTTCTG CATCAACTCC ACCTCGTCGT 
0121 ATAGCTCGGG AGCACTCAGC GCTGTCTCGG GAGGATCTCT GCAAGTCGAC TGCGAGTATG 
0181 CACTTGGTTA CATGGCAGTA TATAGGGTTT GCTTTGGCAT GGCCTGC-TT TTTCGCTCTG 
0241 ATGTCACTGA TTATGCTGGG TGTGAAGAGC TCCA-GGGAT CCGCGTTCGC ACATCCAGAA 
0301 CAACTTTTGG CCACTGAAAT TTCTGAT-CT GCTTTGGCGC TGCGATTGGA GCCATCTTTA 
0361 TTCCGGATGG ATCCTTTGGT CCGGCCATGA TGTGGGTGGG TCTAATTGGT GGCCTGGCCT 
0421 TCATACTCGT CCAGCTGGTC ATCATTGTGG ATTTCGCTCA CTCGCTGGCT GAAAACTGGA 
0481 TAGAAAGCGC CGAAAACAGT CGT 
 Assemble Data 
<PRE>

4672R-3.IR_full       1   CGCGCTTCATGTACGCCTTCATCCTCCTGGTGGGCACCGTACTGGGGGCGATTGCGCTGT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-3.in silico     1   CGCGCTTCATGTACGCCTTCATCCTCCTGGTGGGCACCGTACTGGGGGCGATTGCGCTGT 60


4672R-3.IR_full       61  CCCCTGGACTGCAG<span class="snp"><tt>N</tt></span>ACACCCTGAAGAAGATGCCCTTCTGCATCAACTCCACCTCGTCGT 120
                          ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||
4672R-3.in silico     61  CCCCTGGACTGCAG<span class="snp"><tt>G</tt></span>ACACCCTGAAGAAGATGCCCTTCTGCATCAACTCCACCTCGTCGT 120


4672R-3.IR_full       121 ATAGCTCGGGAGCACTCAGCGCTGTCTCGGGAGGATCTCTGCAAGTCGACTGCGAGTATG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-3.in silico     121 ATAGCTCGGGAGCACTCAGCGCTGTCTCGGGAGGATCTCTGCAAGTCGACTGCGAGTATG 180


4672R-3.IR_full       181 CACTTGGTTACATGGCAGTATATAGGGTTTGCTTTGGCATGGCCTGC<span class="snp"><tt>T</tt></span>TTTTTCGCTCTG 240
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
4672R-3.in silico     181 CACTTGGTTACATGGCAGTATATAGGGTTTGCTTTGGCATGGCCTGC<span class="snp"><tt>-</tt></span>TTTTTCGCTCTG 240


4672R-3.IR_full       241 ATGTCACTGATTATGCTGGGTGTGAAGAGCTCCA<span class="snp"><tt>N</tt></span>GGGATCCGCGTTCGCACATCCAGAA 300
                          ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||
4672R-3.in silico     241 ATGTCACTGATTATGCTGGGTGTGAAGAGCTCCA<span class="snp"><tt>-</tt></span>GGGATCCGCGTTCGCACATCCAGAA 300


4672R-3.IR_full       301 CAACTTTTGGCCACTGAAATTTCTGAT<span class="snp"><tt>C</tt></span>CTGCTTTGG<span class="snp"><tt>N</tt></span>GCTGCGATTGGAGCCATCTTTA 360
                          |||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
4672R-3.in silico     301 CAACTTTTGGCCACTGAAATTTCTGAT<span class="snp"><tt>-</tt></span>CTGCTTTGG<span class="snp"><tt>C</tt></span>GCTGCGATTGGAGCCATCTTTA 360


4672R-3.IR_full       361 TTCCGGATGGATCCTTTGGTCCGGCCATGATGTGGGTGGGTCTAATTGGTGGCCTGGCCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-3.in silico     361 TTCCGGATGGATCCTTTGGTCCGGCCATGATGTGGGTGGGTCTAATTGGTGGCCTGGCCT 420


4672R-3.IR_full       421 TCATACTCGTCCAGCTGGTCATCATTGTGGATTTCGCTCACTCGCTGGCTGAAAACTGGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-3.in silico     421 TCATACTCGTCCAGCTGGTCATCATTGTGGATTTCGCTCACTCGCTGGCTGAAAACTGGA 480


4672R-3.IR_full       481 TAGAAAGCGCCGAAAACAGTCGT 503
                          |||||||||||||||||||||||
4672R-3.in silico     481 TAGAAAGCGCCGAAAACAGTCGT 503

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140636.2  CG4672-RA (TMS1), mRNA 
0   0  3  2  11  NM_169845.1  CG31043-RA, transcript variant A (gukh), mRNA 
0   0  2  7  15  NM_078867.2  CG5526-RA (Dhc36C), mRNA 
0   0  2  4  6  NM_139531.3  CG32269-RA, transcript variant A (CG32269), mRNA 
0   0  2  4  6  NM_168003.2  CG32270-RA, transcript variant A (CG32270), mRNA 
0   0  2  3  5  NM_206295.1  CG33275-RB, transcript variant B (CG33275), mRNA 
0   0  2  3  5  NM_206294.1  CG33275-RA, transcript variant A (CG33275), mRNA 
0   0  2  2  2  NM_170407.2  CG11958-RA, transcript variant A (Cnx99A), mRNA 
0   0  2  2  2  NM_057809.4  CG11958-RB, transcript variant B (Cnx99A), mRNA 
0   0  2  2  2  NM_001043301.1  CG11958-RC, transcript variant C (Cnx99A), mRNA 
0   0  2  2  2  NM_001043302.1  CG11958-RD, transcript variant D (Cnx99A), mRNA 
0   0  1  4  3  NM_140189.1  CG7616-RA (CG7616), mRNA 
0   0  1  3  7  NM_135923.2  CG18801-RA (Ku80), mRNA 
0   0  1  3  5  NM_165974.2  CG4654-RB, transcript variant B (Dp), mRNA 
0   0  1  3  5  NM_057691.3  CG4654-RA, transcript variant A (Dp), mRNA 
0   0  1  3  3  NM_166297.1  CG5327-RA (CG5327), mRNA 
0   0  1  3  3  NM_057627.3  CG8996-RB, transcript variant B (wal), mRNA 
0   0  1  3  3  NM_165847.1  CG8996-RA, transcript variant A (wal), mRNA 
0   0  1  3  2  NM_168926.1  CG32441-RA, transcript variant A (CG32441), mRNA 
0   0  1  3  2  NM_137504.1  CG5493-RA (CG5493), mRNA 
0   0  1  3  2  NM_168925.1  CG32441-RB, transcript variant B (CG32441), mRNA 
0   0  1  3  2  NM_001038888.1  CG34018-RA (CG34018), mRNA 
0   0  1  2  11  NM_057643.2  CG10844-RA, transcript variant A (Rya-r44F), mRNA 
0   0  1  2  11  NM_057644.2  CG10844-RB, transcript variant B (Rya-r44F), mRNA 
0   0  1  2  10  NM_057645.2  CG10844-RC, transcript variant C (Rya-r44F), mRNA 
0   0  1  2  10  NM_057646.2  CG10844-RD, transcript variant D (Rya-r44F), mRNA 
0   0  1  2  5  NM_001032403.1  CG7649-RB, transcript variant B (Neu3), mRNA 
0   0  1  2  5  NM_001032404.1  CG7649-RA, transcript variant A (Neu3), mRNA 
0   0  1  2  5  NM_057602.3  CG2248-RA (Rac1), mRNA 
0   0  1  2  4  NM_078980.2  CG8972-RA (rho-7), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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