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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4672R-1

Symbol

TMS1

Full Name

TMS1

CG No

CG4672

Old CG No

CG4672

Synonyms

TMS1d, CG4672, TMS1

Accession No (Link to NCBI)

NM_140636.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCGCTTCATGTACGCCTTCA

Primer Seq. 3'

aatctagaggtaccACGACTGTTTTCGGCGCTTTC

Predicted Fragment Size

IR fragment full Seq

0001 CGCGCTTCAT GTACGCCTTC ATCCTCCTGG TGGGCACCGT ACTGGGGGCG ATTGCGCTGT 
0061 CCCCTGGACT GCAGNACACC CTGAAGAAGA TGCCCTTCTG CATCAACTCC ACCTCGTCGT 
0121 ATAGCTCGGG AGCACTCAGC GCTGTCTCGG GAGGATCTCT GCAAGTCGAC TGCGAGTATG 
0181 CACTTGGTTA CATGGCAGTA TATAGGGTTT GCTTTGGCAT GGCCTGCTTT TTTCGCTCTG 
0241 ATGTCACTGA TTATGCTGGG TGTGAAGAGC TCCANGGGAT CCGCGTTCGC ACATCCAGAA 
0301 CAACTTTTGG CCACTGAAAT TTCTGATCCT GCTTTGGNGC TGCGATTGGA GCCATCTTTA 
0361 TTCCGGATGG ATCCTTTGGT CCGGCCATGA TGTGGGTGGG TCTAATTGGT gGCCTGGCCT 
0421 TCATACTCGT CCAGCTGGTC ATCATTGTGG ATTTCGCTCA CTCGCTGGCT GAAAACTGGA 
0481 TAGAAAGCGC CGAAAACAGT CGT

in silico PCR Fragment

0001 CGCGCTTCAT GTACGCCTTC ATCCTCCTGG TGGGCACCGT ACTGGGGGCG ATTGCGCTGT 
0061 CCCCTGGACT GCAGGACACC CTGAAGAAGA TGCCCTTCTG CATCAACTCC ACCTCGTCGT 
0121 ATAGCTCGGG AGCACTCAGC GCTGTCTCGG GAGGATCTCT GCAAGTCGAC TGCGAGTATG 
0181 CACTTGGTTA CATGGCAGTA TATAGGGTTT GCTTTGGCAT GGCCTGC-TT TTTCGCTCTG 
0241 ATGTCACTGA TTATGCTGGG TGTGAAGAGC TCCA-GGGAT CCGCGTTCGC ACATCCAGAA 
0301 CAACTTTTGG CCACTGAAAT TTCTGAT-CT GCTTTGGCGC TGCGATTGGA GCCATCTTTA 
0361 TTCCGGATGG ATCCTTTGGT CCGGCCATGA TGTGGGTGGG TCTAATTGGT GGCCTGGCCT 
0421 TCATACTCGT CCAGCTGGTC ATCATTGTGG ATTTCGCTCA CTCGCTGGCT GAAAACTGGA 
0481 TAGAAAGCGC CGAAAACAGT CGT

Assemble Data

<PRE>

4672R-1.IR_full       1   CGCGCTTCATGTACGCCTTCATCCTCCTGGTGGGCACCGTACTGGGGGCGATTGCGCTGT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-1.in silico     1   CGCGCTTCATGTACGCCTTCATCCTCCTGGTGGGCACCGTACTGGGGGCGATTGCGCTGT 60


4672R-1.IR_full       61  CCCCTGGACTGCAG<span class="snp"><tt>N</tt></span>ACACCCTGAAGAAGATGCCCTTCTGCATCAACTCCACCTCGTCGT 120
                          ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||
4672R-1.in silico     61  CCCCTGGACTGCAG<span class="snp"><tt>G</tt></span>ACACCCTGAAGAAGATGCCCTTCTGCATCAACTCCACCTCGTCGT 120


4672R-1.IR_full       121 ATAGCTCGGGAGCACTCAGCGCTGTCTCGGGAGGATCTCTGCAAGTCGACTGCGAGTATG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-1.in silico     121 ATAGCTCGGGAGCACTCAGCGCTGTCTCGGGAGGATCTCTGCAAGTCGACTGCGAGTATG 180


4672R-1.IR_full       181 CACTTGGTTACATGGCAGTATATAGGGTTTGCTTTGGCATGGCCTGC<span class="snp"><tt>T</tt></span>TTTTTCGCTCTG 240
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
4672R-1.in silico     181 CACTTGGTTACATGGCAGTATATAGGGTTTGCTTTGGCATGGCCTGC<span class="snp"><tt>-</tt></span>TTTTTCGCTCTG 240


4672R-1.IR_full       241 ATGTCACTGATTATGCTGGGTGTGAAGAGCTCCA<span class="snp"><tt>N</tt></span>GGGATCCGCGTTCGCACATCCAGAA 300
                          ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||
4672R-1.in silico     241 ATGTCACTGATTATGCTGGGTGTGAAGAGCTCCA<span class="snp"><tt>-</tt></span>GGGATCCGCGTTCGCACATCCAGAA 300


4672R-1.IR_full       301 CAACTTTTGGCCACTGAAATTTCTGAT<span class="snp"><tt>C</tt></span>CTGCTTTGG<span class="snp"><tt>N</tt></span>GCTGCGATTGGAGCCATCTTTA 360
                          |||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
4672R-1.in silico     301 CAACTTTTGGCCACTGAAATTTCTGAT<span class="snp"><tt>-</tt></span>CTGCTTTGG<span class="snp"><tt>C</tt></span>GCTGCGATTGGAGCCATCTTTA 360


4672R-1.IR_full       361 TTCCGGATGGATCCTTTGGTCCGGCCATGATGTGGGTGGGTCTAATTGGTGGCCTGGCCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-1.in silico     361 TTCCGGATGGATCCTTTGGTCCGGCCATGATGTGGGTGGGTCTAATTGGTGGCCTGGCCT 420


4672R-1.IR_full       421 TCATACTCGTCCAGCTGGTCATCATTGTGGATTTCGCTCACTCGCTGGCTGAAAACTGGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4672R-1.in silico     421 TCATACTCGTCCAGCTGGTCATCATTGTGGATTTCGCTCACTCGCTGGCTGAAAACTGGA 480


4672R-1.IR_full       481 TAGAAAGCGCCGAAAACAGTCGT 503
                          |||||||||||||||||||||||
4672R-1.in silico     481 TAGAAAGCGCCGAAAACAGTCGT 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140636.2	CG4672-RA (TMS1), mRNA
0	0	3	2	11	NM_169845.1	CG31043-RA, transcript variant A (gukh), mRNA
0	0	2	7	15	NM_078867.2	CG5526-RA (Dhc36C), mRNA
0	0	2	4	6	NM_139531.3	CG32269-RA, transcript variant A (CG32269), mRNA
0	0	2	4	6	NM_168003.2	CG32270-RA, transcript variant A (CG32270), mRNA
0	0	2	3	5	NM_206295.1	CG33275-RB, transcript variant B (CG33275), mRNA
0	0	2	3	5	NM_206294.1	CG33275-RA, transcript variant A (CG33275), mRNA
0	0	2	2	2	NM_170407.2	CG11958-RA, transcript variant A (Cnx99A), mRNA
0	0	2	2	2	NM_057809.4	CG11958-RB, transcript variant B (Cnx99A), mRNA
0	0	2	2	2	NM_001043301.1	CG11958-RC, transcript variant C (Cnx99A), mRNA
0	0	2	2	2	NM_001043302.1	CG11958-RD, transcript variant D (Cnx99A), mRNA
0	0	1	4	3	NM_140189.1	CG7616-RA (CG7616), mRNA
0	0	1	3	7	NM_135923.2	CG18801-RA (Ku80), mRNA
0	0	1	3	5	NM_165974.2	CG4654-RB, transcript variant B (Dp), mRNA
0	0	1	3	5	NM_057691.3	CG4654-RA, transcript variant A (Dp), mRNA
0	0	1	3	3	NM_166297.1	CG5327-RA (CG5327), mRNA
0	0	1	3	3	NM_057627.3	CG8996-RB, transcript variant B (wal), mRNA
0	0	1	3	3	NM_165847.1	CG8996-RA, transcript variant A (wal), mRNA
0	0	1	3	2	NM_168926.1	CG32441-RA, transcript variant A (CG32441), mRNA
0	0	1	3	2	NM_137504.1	CG5493-RA (CG5493), mRNA
0	0	1	3	2	NM_168925.1	CG32441-RB, transcript variant B (CG32441), mRNA
0	0	1	3	2	NM_001038888.1	CG34018-RA (CG34018), mRNA
0	0	1	2	11	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA
0	0	1	2	11	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	1	2	10	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	1	2	10	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	1	2	5	NM_001032403.1	CG7649-RB, transcript variant B (Neu3), mRNA
0	0	1	2	5	NM_001032404.1	CG7649-RA, transcript variant A (Neu3), mRNA
0	0	1	2	5	NM_057602.3	CG2248-RA (Rac1), mRNA
0	0	1	2	4	NM_078980.2	CG8972-RA (rho-7), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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