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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4659R-4

Symbol

Srp54k

Full Name

Signal recognition particle protein 54k

CG No

CG4659

Old CG No

CG4659

Synonyms

CG4659, Srp54, l(3)01418, BcDNA.GM09489, BcDNA:GM09489, Srp54k

Accession No (Link to NCBI)

NM_079207.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGTTCTCGCGGATTTGGGT

Primer Seq. 3'

aatctagaggtaccTCGATTTCCGTGTAGCTGCCG

Predicted Fragment Size

IR fragment full Seq

0001 ATGGTTCTCG CGGATTTGGG TCGCAAGATA ACAACGGCGC TGCACTCGCT CAGCAAGGCG 
0061 ACCGTAATCA ATGAGGAGGC ACTGAATTCC ATGCTGAAGG AGATCTGCGC AGCgCTCNCT 
0121 GGAGGCTGAT GTGAATATCC GACTGGTTAA GCAGCTGCGC GANGAATGTG CGTGCCGTTA 
0181 TTGATTTCGA TGAGATGGCC GGTGGCCTGA ACAAGAGGCG AATGATCCAA TCGGCTGTGT 
0241 TCAAGGAGCT CGTCAAACTG GTCGATCCCG GCGTGAAACC CTACCAACCC ATCAAGGGCA 
0301 AGGCCAATGT GGTCATGTTT GTGGGTCTGC AGGGCTCCGG TAAGACCACC ACCTGTACCA 
0361 AGCTGGCCTA TCACTACCAG AAGCGCAACT GGAAGTCGTG TCTCGTGTGC GCGGATACCT 
0421 TCCGTGCTGG TGCTTACGAT CAGGTCAAGC AGAACGCCAC CAAGGCGCGC ATTCCGTTCT 
0481 ACGGCAGCTA CACGGAAATC GA

in silico PCR Fragment

0001 ATGGTTCTCG CGGATTTGGG TCGCAAGATA ACAACGGCGC TGCACTCGCT CAGCAAGGCG 
0061 ACCGTAATCA ATGAGGAGGC ACTGAATTCC ATGCTGAAGG AGATCTGCGC AGCGCTC-CT 
0121 GGAGGCTGAT GTGAATATCC GACTGGTTAA GCAGCTGCGC GA-GAATGTG CGTGCCGTTA 
0181 TTGATTTCGA TGAGATGGCC GGTGGCCTGA ACAAGAGGCG AATGATCCAA TCGGCTGTGT 
0241 TCAAGGAGCT CGTCAAACTG GTCGATCCCG GCGTGAAACC CTACCAACCC ATCAAGGGCA 
0301 AGGCCAATGT GGTCATGTTT GTGGGTCTGC AGGGCTCCGG TAAGACCACC ACCTGTACCA 
0361 AGCTGGCCTA TCACTACCAG AAGCGCAACT GGAAGTCGTG TCTCGTGTGC GCGGATACCT 
0421 TCCGTGCTGG TGCCTACGAT CAGGTCAAGC AGAACGCCAC CAAGGCGCGC ATTCCGTTCT 
0481 ACGGCAGCTA CACGGAAATC GA

Assemble Data

<PRE>

4659R-4.IR_full       1   ATGGTTCTCGCGGATTTGGGTCGCAAGATAACAACGGCGCTGCACTCGCTCAGCAAGGCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4659R-4.in silico     1   ATGGTTCTCGCGGATTTGGGTCGCAAGATAACAACGGCGCTGCACTCGCTCAGCAAGGCG 60


4659R-4.IR_full       61  ACCGTAATCAATGAGGAGGCACTGAATTCCATGCTGAAGGAGATCTGCGCAGCGCTC<span class="snp"><tt>N</tt></span>CT 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
4659R-4.in silico     61  ACCGTAATCAATGAGGAGGCACTGAATTCCATGCTGAAGGAGATCTGCGCAGCGCTC<span class="snp"><tt>-</tt></span>CT 120


4659R-4.IR_full       121 GGAGGCTGATGTGAATATCCGACTGGTTAAGCAGCTGCGCGA<span class="snp"><tt>N</tt></span>GAATGTGCGTGCCGTTA 180
                          ||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
4659R-4.in silico     121 GGAGGCTGATGTGAATATCCGACTGGTTAAGCAGCTGCGCGA<span class="snp"><tt>-</tt></span>GAATGTGCGTGCCGTTA 180


4659R-4.IR_full       181 TTGATTTCGATGAGATGGCCGGTGGCCTGAACAAGAGGCGAATGATCCAATCGGCTGTGT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4659R-4.in silico     181 TTGATTTCGATGAGATGGCCGGTGGCCTGAACAAGAGGCGAATGATCCAATCGGCTGTGT 240


4659R-4.IR_full       241 TCAAGGAGCTCGTCAAACTGGTCGATCCCGGCGTGAAACCCTACCAACCCATCAAGGGCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4659R-4.in silico     241 TCAAGGAGCTCGTCAAACTGGTCGATCCCGGCGTGAAACCCTACCAACCCATCAAGGGCA 300


4659R-4.IR_full       301 AGGCCAATGTGGTCATGTTTGTGGGTCTGCAGGGCTCCGGTAAGACCACCACCTGTACCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4659R-4.in silico     301 AGGCCAATGTGGTCATGTTTGTGGGTCTGCAGGGCTCCGGTAAGACCACCACCTGTACCA 360


4659R-4.IR_full       361 AGCTGGCCTATCACTACCAGAAGCGCAACTGGAAGTCGTGTCTCGTGTGCGCGGATACCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4659R-4.in silico     361 AGCTGGCCTATCACTACCAGAAGCGCAACTGGAAGTCGTGTCTCGTGTGCGCGGATACCT 420


4659R-4.IR_full       421 TCCGTGCTGGTGC<span class="snp"><tt>T</tt></span>TACGATCAGGTCAAGCAGAACGCCACCAAGGCGCGCATTCCGTTCT 480
                          |||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||
4659R-4.in silico     421 TCCGTGCTGGTGC<span class="snp"><tt>C</tt></span>TACGATCAGGTCAAGCAGAACGCCACCAAGGCGCGCATTCCGTTCT 480


4659R-4.IR_full       481 ACGGCAGCTACACGGAAATCGA 502
                          ||||||||||||||||||||||
4659R-4.in silico     481 ACGGCAGCTACACGGAAATCGA 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079207.2	CG4659-RA (Srp54k), mRNA
0	0	4	3	2	NM_139651.1	CG11349-RA (CG11349), mRNA
0	0	4	3	2	NM_164830.3	CG18405-RA, transcript variant A (Sema-1a), mRNA
0	0	4	3	2	NM_135399.2	CG18405-RB, transcript variant B (Sema-1a), mRNA
0	0	3	3	3	NM_136661.1	CG12928-RA (CG12928), mRNA
0	0	3	2	2	NM_140772.2	CG7341-RA, transcript variant A (CG7341), mRNA
0	0	3	2	2	NM_168763.1	CG7341-RB, transcript variant B (CG7341), mRNA
0	0	2	5	3	NM_001042852.1	CG12500-RB, transcript variant B (stnA), mRNA
0	0	2	5	3	NM_001042851.1	CG12500-RA, transcript variant A (stnA), mRNA
0	0	2	5	3	NM_001042854.1	CG12473-RB, transcript variant B (stnB), mRNA
0	0	2	5	3	NM_001042853.1	CG12473-RA, transcript variant A (stnB), mRNA
0	0	2	2	2	NM_080380.1	CG4326-RA (mRpS17), mRNA
0	0	1	6	4	NM_140796.1	CG6893-RA (CG6893), mRNA
0	0	1	5	2	NM_136374.2	CG3183-RA (geminin), mRNA
0	0	1	4	9	NM_140650.2	CG18217-RA (CG18217), mRNA
0	0	1	4	9	NM_145191.1	CG4098-RA (CG4098), mRNA
0	0	1	4	8	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	1	4	4	NM_141999.2	CG7966-RA (CG7966), mRNA
0	0	1	4	2	NM_142210.1	CG6118-RA (CG6118), mRNA
0	0	1	4	2	NM_142812.1	CG13843-RA (CG13843), mRNA
0	0	1	3	6	NM_143360.2	CG1345-RA (Gfat2), mRNA
0	0	1	3	2	NM_135946.2	CG4278-RA (CG4278), mRNA
0	0	1	3	2	NM_135505.2	CG4839-RA, transcript variant A (CG4839), mRNA
0	0	1	3	2	NM_164890.1	CG4839-RB, transcript variant B (CG4839), mRNA
0	0	1	2	8	NM_001038739.1	CG9900-RC, transcript variant C (mit(1)15), mRNA
0	0	1	2	8	NM_080162.3	CG9900-RB, transcript variant B (mit(1)15), mRNA
0	0	1	2	4	NM_136715.2	CG18011-RA (CG18011), mRNA
0	0	1	2	4	NM_141297.1	CG2926-RA (CG2926), mRNA
0	0	0	8	32	NM_206687.1	CG33235-RA (CG33235), mRNA
0	0	0	8	5	NM_079609.2	CG6019-RA (mus308), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme03060 : Protein export

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